Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

3.4.21.63: Oryzin

This is an abbreviated version!
For detailed information about Oryzin, go to the full flat file.

Word Map on EC 3.4.21.63

Reaction

Hydrolysis of proteins with broad specificity, and of Bz-Arg-OEt > Ac-Tyr-OEt. Does not hydrolyse peptide amides =

Synonyms

Alkaline protease, Alkaline proteinase, alkaline serine protease, ALP, ALP1, AOP, AP15, AP30, API 21, Aspergillopepsin B, Aspergillopepsin F, Aspergillopeptidase B, Aspergillus alkaline proteinase, Aspergillus candidus alkaline proteinase, Aspergillus flavus alkaline proteinase, Aspergillus melleus semi-alkaline proteinase, Aspergillus oryzae alkaline proteinase, Aspergillus oryzae protease, Aspergillus parasiticus alkaline proteinase, Aspergillus serine proteinase, Aspergillus terreus protease, collagenolytic serine peptidase, EC 3.4.21.14, EC 3.4.21.15, EC 3.4.4.16, elastase, Elastinolytic serine proteinase, Kyorinase, neutral protease I, NpI, Onoprose, Onoprose SA, P 5380, Promelase, protease Alp1, Protease P, Proteinase, Aspergillus alkaline, Proteinase, Aspergillus flavus alkaline, Proteinase, Aspergillus melleus alkaline, Proteinase, Aspergillus oryzae alkaline, Proteinase, Aspergillus parasiticus alkaline, Proteinase, Aspergillus soya alkaline, Proteinase, Aspergillus sulphureus alkaline, Proteinase, Aspergillus sydowi alkaline, Prozyme, Prozyme 10, PrtA, PrtA protease, SapHM, Seaprose S, Semi-alkaline protease, Sumizyme MP

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.21 Serine endopeptidases
                3.4.21.63 Oryzin

Cloned

Cloned on EC 3.4.21.63 - Oryzin

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
gene prtA, DNA and amino acid sequence determination and analysis, expression in Pichia pastoris strain X-33 as inactive enzyme, the presence of this enzyme was toxic for the host and resulted in cell lysis, modification of the culture medium constituents improve the expression by 6.4fold
-
gene sapHM, DNA and amino acid sequence determination and analysis, phylogenetic tree
high-level expression of plasmids pPIC9K-n/Alp and pPIC9K-alpha/Alp in Pichia pastoris GS115 with native signal peptide or alpha-factor secretion signal peptide
overexpression in Escherichia coli strain BL21(DE3)
-
recombinant expression of a truncated neutral protease I in Pichia pastoris with a high enzyme yield
-
recombinant expression of His6-tagged enzyme without signal peptide in Pichia pastoris strain KM71
sequence comparisons between Aspergillus species and different proteases, clustering of alkaline proteases, phylogenetic tree, overview
strain U1521 contains multiple copies of alkaline protease gene
-