3.4.21.2: chymotrypsin C
This is an abbreviated version!
For detailed information about chymotrypsin C, go to the full flat file.
Reaction
Preferential cleavage: Leu-/-, Tyr-/-, Phe-/-, Met-/-, Trp-/-, Gln-/-, Asn-/- =
Synonyms
caldecrin, chymotrypsin C, chymotrypsinogen C, CTRC, elastase-associated acidic endopeptidase, elastase-like chymotrypsin, enzyme Y, Granzyme M, GrzM, Serum calcium-decreasing factor
ECTree
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Cloned
Cloned on EC 3.4.21.2 - chymotrypsin C
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cDNAs for the wild-type CTRC and the p.A73T mutant carrying a Glu-Glu epitope tag excised from pcDNA3.1(-)_CTRC expression plasmids with XhoI and EcoRI and subcloned into the VQ Ad5CMV shuttle vector under the control of a CMV promoter. Dexamethasone-differentiated AR42J rat acinar cells and freshly isolated mouse acini transfected with recombinant adenovirus carrying wild-type CTRC or the p.A73T pancreatitis-associated mutant
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