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Literature summary for 3.4.21.2 extracted from
- Determinants of chymotrypsin C cleavage specificity in the calcium-binding loop of human cationic trypsinogen (2012), FEBS J., 279, 4283-4292.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in HEK-293T cell | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0118 | - |
succinyl-Ala-Ala-Pro-Leu 4-nitroanilide | pH 8.0, temperature not specified in the publication | Homo sapiens |  |
| 0.0196 | - |
succinyl-Ala-Ala-Pro-Tyr 4-nitroanilide | pH 8.0, temperature not specified in the publication | Homo sapiens | |
| 0.022 | - |
succinyl-Ala-Ala-Pro-Phe 4-nitroanilide | pH 8.0, temperature not specified in the publication | Homo sapiens | |
| 0.0277 | - |
succinyl-Ala-Ala-Pro-Trp 4-nitroanilide | pH 8.0, temperature not specified in the publication | Homo sapiens | |
| 0.0304 | - |
succinyl-Ala-Ala-Pro-Met 4-nitroanilide | pH 8.0, temperature not specified in the publication | Homo sapiens | |
| 0.0605 | - |
succinyl-Ala-Ala-Pro-Ile 4-nitroanilide | pH 8.0, temperature not specified in the publication | Homo sapiens | |
| 0.1504 | - |
succinyl-Ala-Ala-Pro-Val 4-nitroanilide | pH 8.0, temperature not specified in the publication | Homo sapiens | |
| 0.392 | - |
succinyl-Ala-Ala-Pro-Ala 4-nitroanilide | pH 8.0, temperature not specified in the publication | Homo sapiens | |
| 0.738 | - |
succinyl-Ala-Ala-Pro-Gln 4-nitroanilide | pH 8.0, temperature not specified in the publication | Homo sapiens | |
| 3.452 | - |
succinyl-Ala-Ala-Pro-Asn 4-nitroanilide | pH 8.0, temperature not specified in the publication | Homo sapiens | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | Q99895 | - |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| human cationic trypsinogen + H2O | - |
Homo sapiens | ? | enzyme cleaves the Leu81-Glu82 peptide bond in the calcium-binding loop. Chymotrypsin C-mediated cleavage is stimulated threefold by mutation E82A and unaffected by mutations E79A and N84A in human cationic trypsinogen. Specific cleavage of the Leu81-Glu82 peptide bond is primarily determined by the enzymes' distinctively high activity on leucyl peptide bonds, with the P1' Glu82, P3' Asn84 and P4' Glu85 residues serving as additional specificity determinants | ? | |
| additional information | CTRC cleaves after a P1 Leu with at least tenfold higher catalytic efficiency than other enzymes tested | Homo sapiens | ? | - |
? | |
| succinyl-Ala-Ala-Pro-Ala 4-nitroanilide + H2O | - |
Homo sapiens | succinyl-Ala-Ala-Pro-Ala + 4-nitroaniline | - |
? | |
| succinyl-Ala-Ala-Pro-Asn 4-nitroanilide + H2O | - |
Homo sapiens | succinyl-Ala-Ala-Pro-Asn + 4-nitroaniline | - |
? | |
| succinyl-Ala-Ala-Pro-Gln 4-nitroanilide + H2O | - |
Homo sapiens | succinyl-Ala-Ala-Pro-Gln + 4-nitroaniline | - |
? | |
| succinyl-Ala-Ala-Pro-Ile 4-nitroanilide + H2O | - |
Homo sapiens | succinyl-Ala-Ala-Pro-Ile + 4-nitroaniline | - |
? | |
| succinyl-Ala-Ala-Pro-Leu 4-nitroanilide + H2O | - |
Homo sapiens | succinyl-Ala-Ala-Pro-Leu + 4-nitroaniline | - |
? | |
| succinyl-Ala-Ala-Pro-Met 4-nitroanilide + H2O | - |
Homo sapiens | succinyl-Ala-Ala-Pro-Met + 4-nitroaniline | - |
? | |
| succinyl-Ala-Ala-Pro-Phe 4-nitroanilide + H2O | - |
Homo sapiens | succinyl-Ala-Ala-Pro-Phe + 4-nitroaniline | - |
? | |
| succinyl-Ala-Ala-Pro-Trp 4-nitroanilide + H2O | - |
Homo sapiens | succinyl-Ala-Ala-Pro-Trp + 4-nitroaniline | - |
? | |
| succinyl-Ala-Ala-Pro-Tyr 4-nitroanilide + H2O | - |
Homo sapiens | succinyl-Ala-Ala-Pro-Tyr + 4-nitroaniline | - |
? | |
| succinyl-Ala-Ala-Pro-Val 4-nitroanilide + H2O | - |
Homo sapiens | succinyl-Ala-Ala-Pro-Val + 4-nitroaniline | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CTRC | - |
Homo sapiens |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.03 | - |
succinyl-Ala-Ala-Pro-Ile 4-nitroanilide | pH 8.0, temperature not specified in the publication | Homo sapiens | |
| 0.05 | - |
succinyl-Ala-Ala-Pro-Val 4-nitroanilide | pH 8.0, temperature not specified in the publication | Homo sapiens | |
| 0.1 | - |
succinyl-Ala-Ala-Pro-Trp 4-nitroanilide | pH 8.0, temperature not specified in the publication | Homo sapiens | |
| 0.3 | - |
succinyl-Ala-Ala-Pro-Ala 4-nitroanilide | pH 8.0, temperature not specified in the publication | Homo sapiens | |
| 1.1 | - |
succinyl-Ala-Ala-Pro-Gln 4-nitroanilide | pH 8.0, temperature not specified in the publication | Homo sapiens | |
| 3.6 | - |
succinyl-Ala-Ala-Pro-Asn 4-nitroanilide | pH 8.0, temperature not specified in the publication | Homo sapiens | |
| 9.2 | - |
succinyl-Ala-Ala-Pro-Leu 4-nitroanilide | pH 8.0, temperature not specified in the publication | Homo sapiens | |
| 13.2 | - |
succinyl-Ala-Ala-Pro-Phe 4-nitroanilide | pH 8.0, temperature not specified in the publication | Homo sapiens | |
| 14.9 | - |
succinyl-Ala-Ala-Pro-Met 4-nitroanilide | pH 8.0, temperature not specified in the publication | Homo sapiens | |
| 16.6 | - |
succinyl-Ala-Ala-Pro-Tyr 4-nitroanilide | pH 8.0, temperature not specified in the publication | Homo sapiens | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.33 | - |
succinyl-Ala-Ala-Pro-Val 4-nitroanilide | pH 8.0, temperature not specified in the publication | Homo sapiens | |
| 0.5 | - |
succinyl-Ala-Ala-Pro-Ile 4-nitroanilide | pH 8.0, temperature not specified in the publication | Homo sapiens | |
| 0.77 | - |
succinyl-Ala-Ala-Pro-Ala 4-nitroanilide | pH 8.0, temperature not specified in the publication | Homo sapiens | |
| 1 | - |
succinyl-Ala-Ala-Pro-Asn 4-nitroanilide | pH 8.0, temperature not specified in the publication | Homo sapiens | |
| 1.5 | - |
succinyl-Ala-Ala-Pro-Gln 4-nitroanilide | pH 8.0, temperature not specified in the publication | Homo sapiens | |
| 3.6 | - |
succinyl-Ala-Ala-Pro-Trp 4-nitroanilide | pH 8.0, temperature not specified in the publication | Homo sapiens | |
| 490 | - |
succinyl-Ala-Ala-Pro-Met 4-nitroanilide | pH 8.0, temperature not specified in the publication | Homo sapiens | |
| 600 | - |
succinyl-Ala-Ala-Pro-Phe 4-nitroanilide | pH 8.0, temperature not specified in the publication | Homo sapiens | |
| 780 | - |
succinyl-Ala-Ala-Pro-Leu 4-nitroanilide | pH 8.0, temperature not specified in the publication | Homo sapiens | |
| 850 | - |
succinyl-Ala-Ala-Pro-Tyr 4-nitroanilide | pH 8.0, temperature not specified in the publication | Homo sapiens | |
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