3.4.21.2: chymotrypsin C

This is an abbreviated version!
For detailed information about chymotrypsin C, go to the full flat file.

Reaction

Preferential cleavage: Leu-/-, Tyr-/-, Phe-/-, Met-/-, Trp-/-, Gln-/-, Asn-/- =

Synonyms

caldecrin, chymotrypsin C, chymotrypsinogen C, CTRC, elastase-associated acidic endopeptidase, elastase-like chymotrypsin, enzyme Y, Granzyme M, GrzM, Serum calcium-decreasing factor

ECTree

     3 Hydrolases

         3.4 Acting on peptide bonds (peptidases)

             3.4.21 Serine endopeptidases

                3.4.21.2 chymotrypsin C

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Results	in table
7	AA Sequence
1	Application
1	CAS Registry Number
5	Cloned(Commentary)
1	Crystallization (Commentary)
182	Disease/ Diagnostics
1	Expression
5	General Information
1	General Stability
4	Inhibitors
34	kcat/KM [mM/s]
48	KM Value [mM]
3	Molecular Weight [Da]
2	Natural Substrates/ Products (Substrates)
20	Organism
2	PDB ID
4	Posttranslational Modification
34	Protein Variants
3	Purification (Commentary)
1	Reaction
3	Reaction Type
20	Reference
1	Renatured (Commentary)
8	Source Tissue
48	Substrates and Products (Substrate)
2	Subunits
18	Synonyms
47	Turnover Number [1/s]

Substrates Products

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Substrates Products on EC 3.4.21.2 - chymotrypsin C

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SUBSTRATE

PRODUCT                       

REACTION DIAGRAM

ORGANISM

UNIPROT

COMMENTARY
(Substrate)

LITERATURE
(Substrate)

COMMENTARY
(Product)

LITERATURE
(Product)

Reversibility
r=reversible
ir=irreversible
?=not specified

A chain of oxidized insulin + H2O

?

Sus scrofa

-

-

95320

-

-

?

acetyl-L-Leu methyl ester + H2O

acetyl-L-Leu + methanol

Sus scrofa

-

-

95319

-

-

?

acetyl-L-Trp ethyl ester + H2O

acetyl-L-Trp + ethanol

Sus scrofa

-

-

95319, 95320

-

-

?

anionic trypsinogen + H2O

?

Homo sapiens

Q99895

-

754134

-

-

?

B chain of oxidized insulin + H2O

?

Sus scrofa

-

-

95320

-

-

?

benzoyl-L-Leu methyl ester + H2O

benzoyl-L-Leu + methanol

Sus scrofa

-

-

95319

-

-

?

benzoyl-L-Met ethyl ester + H2O

benzoyl-L-Met + ethanol

Sus scrofa

-

-

95320

-

-

?

benzoyl-L-Tyr ethyl ester + H2O

benzoyl-L-Tyr + ethanol

Sus scrofa

-

-

95319, 95320

-

-

?

benzoyl-Leu-ethyl ester + H2O

benzoyl-Leu + ethanol

Sus scrofa

-

-

95320

-

-

?

benzoyl-Phe ethyl ester + H2O

benzoyl-Phe + ethanol

Sus scrofa

-

-

95319, 95320

-

-

?

benzyloxycarbonyl-L-Leu-Gly-amide + H2O

benzyloxycarbonyl-L-Leu-Gly + NH3

Sus scrofa

-

-

95320

-

-

?

beta-casein + H2O

?

Homo sapiens

Q99895

-

754977

-

-

?

carboxymethylated reduced ribonuclease + H2O

?

Sus scrofa

-

-

95320

-

-

?

cationic trypsin + H2O

?

Homo sapiens

Q99895

-

682546

selectively cleaves the Leu81-Glu82 peptide bond within the Ca2-binding loop

-

?

cationic trypsinogen + H2O

?

Homo sapiens

-

chymotrypsin activates trypsinogen by cleaving the N-terminal tripeptide of the protein

669441

-

-

?

cationic trypsinogen A16V + H2O

?

Homo sapiens

-

the A16V mutation of trypsinogen is processed 4fold more rapidly compared to wild-type trypsiongen

669441

-

-

?

Glucagon + H2O

?

Sus scrofa

-

-

95320

-

-

?

human cationic trypsinogen + H2O

?

2 entries

N-acetyl-Tyr ethyl ester + H2O

N-acetyl-Tyr + ethanol

Sus scrofa

-

-

95318, 95319

-

-

?

N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide + H2O

N-succinyl-Ala-Ala-Pro-Phe + 4-nitroaniline

Homo sapiens

Q99895

-

754977

-

-

?

N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide + H2O

N-succinyl-Ala-Ala-Pro-Phe + p-nitroaniline

2 entries

N-succinyl-L-Ala-Ala-Pro-Leu-p-nitroanilide + H2O

N-succinyl-L-Ala-Ala-Pro-Leu + p-nitroaniline

Conogethes punctiferalis

-

-

680126

-

-

?

oxidized oxytocin + H2O

?

Sus scrofa

-

-

95320

-

-

?

p-nitrophenyl acetate + H2O

p-nitrophenol + acetate

Sus scrofa

-

-

95320

-

-

?

Ser-His-Leu-Val-Glu + H2O

?

Sus scrofa

-

-

95320

-

-

?

succinyl-Ala-Ala-Pro-Ala 4-nitroanilide + H2O

succinyl-Ala-Ala-Pro-Ala + 4-nitroaniline

Homo sapiens

Q99895

-

731789

-

-

?

succinyl-Ala-Ala-Pro-Asn 4-nitroanilide + H2O

succinyl-Ala-Ala-Pro-Asn + 4-nitroaniline

Homo sapiens

Q99895

-

731789

-

-

?

succinyl-Ala-Ala-Pro-Gln 4-nitroanilide + H2O

succinyl-Ala-Ala-Pro-Gln + 4-nitroaniline

Homo sapiens

Q99895

-

731789

-

-

?

succinyl-Ala-Ala-Pro-Ile 4-nitroanilide + H2O

succinyl-Ala-Ala-Pro-Ile + 4-nitroaniline

Homo sapiens

Q99895

-

731789

-

-

?

succinyl-Ala-Ala-Pro-Leu 4-nitroanilide + H2O

succinyl-Ala-Ala-Pro-Leu + 4-nitroaniline

Homo sapiens

Q99895

-

731789

-

-

?

succinyl-Ala-Ala-Pro-Met 4-nitroanilide + H2O

succinyl-Ala-Ala-Pro-Met + 4-nitroaniline

Homo sapiens

Q99895

-

731789

-

-

?

succinyl-Ala-Ala-Pro-Phe 4-nitroanilide + H2O

succinyl-Ala-Ala-Pro-Phe + 4-nitroaniline

2 entries

succinyl-Ala-Ala-Pro-Phe-4-nitroanilide + H2O

succinyl-Ala-Ala-Pro-Phe + 4-nitroaniline

Homo sapiens

Q99895

-

754111

-

-

?

succinyl-Ala-Ala-Pro-Trp 4-nitroanilide + H2O

succinyl-Ala-Ala-Pro-Trp + 4-nitroaniline

Homo sapiens

Q99895

-

731789

-

-

?

succinyl-Ala-Ala-Pro-Tyr 4-nitroanilide + H2O

succinyl-Ala-Ala-Pro-Tyr + 4-nitroaniline

Homo sapiens

Q99895

-

731789

-

-

?

succinyl-Ala-Ala-Pro-Val 4-nitroanilide + H2O

succinyl-Ala-Ala-Pro-Val + 4-nitroaniline

Homo sapiens

Q99895

-

731789

-

-

?

Trypsinogen + H2O

?

2 entries

additional information

?

-

4 entries

human cationic trypsinogen + H2O

?

Homo sapiens

Q99895

-

754977

-

-

?

human cationic trypsinogen + H2O

?

Homo sapiens

Q99895

-

731789

enzyme cleaves the Leu81-Glu82 peptide bond in the calcium-binding loop. Chymotrypsin C-mediated cleavage is stimulated threefold by mutation E82A and unaffected by mutations E79A and N84A in human cationic trypsinogen. Specific cleavage of the Leu81-Glu82 peptide bond is primarily determined by the enzymes' distinctively high activity on leucyl peptide bonds, with the P1' Glu82, P3' Asn84 and P4' Glu85 residues serving as additional specificity determinants

-

?

N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide + H2O

N-succinyl-Ala-Ala-Pro-Phe + p-nitroaniline

Homo sapiens

-

-

669441

-

-

?

N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide + H2O

N-succinyl-Ala-Ala-Pro-Phe + p-nitroaniline

Homo sapiens

Q99895

-

682546

-

-

?

succinyl-Ala-Ala-Pro-Phe 4-nitroanilide + H2O

succinyl-Ala-Ala-Pro-Phe + 4-nitroaniline

Homo sapiens

Q99895

-

731789, 731886

-

-

?

succinyl-Ala-Ala-Pro-Phe 4-nitroanilide + H2O

succinyl-Ala-Ala-Pro-Phe + 4-nitroaniline

Homo sapiens

-

-

731886

-

-

?

Trypsinogen + H2O

?

Homo sapiens

Q99895

-

682546

-

-

?

Trypsinogen + H2O

?

Homo sapiens

Q99895

chymotrypsin C protects against pancreatitis by degrading trypsinogen and thereby curtailing harmful intrapancreatic trypsinogen activation

754111

-

-

?

additional information

?

-

Conogethes punctiferalis

-

no activity with N-benzoyl-L-Tyr-p-nitroanilide, L-Leu-p-nitroanilide, and N-benzoyl-L-Arg-p-nitroanilide

680126

-

-

?

additional information

?

-

Homo sapiens

Q99895

CTRC cleaves after a P1 Leu with at least tenfold higher catalytic efficiency than other enzymes tested

731789

-

-

?

additional information

?

-

Homo sapiens

-

CTRC cleaves after a P1 Leu with at least tenfold higher catalytic efficiency than other enzymes tested

731789

-

-

?

additional information

?

-

Sus scrofa

-

hydrolysis of Tyr, Phe, Met, Trp, Leu, Glu, and Asp

95321

-

-

?