3.4.21.100: sedolisin

This is an abbreviated version!
For detailed information about sedolisin, go to the full flat file.

Word Map on EC 3.4.21.100

3.4.21.100

peptidase

proteinases

subtilisins

merops

ceroid

pcp

lipofuscinosis

tripeptidyl-peptidase

diazoacetyl-dl-norleucine

subtilisin-like

prosegment

tripeptidyl

b-chain

1,2-epoxy-3-p-nitrophenoxypropane

coagulans

medicine

Reaction

Hydrolysis of the B chain of insulin at -Glu13-/-Ala-, -Leu15-/-Tyr- and -Phe25-/-Tyr-, and angiotensin I at -Tyr4-/-Ile-. A good synthetic substrate is Lys-Pro-Ile-Glu-Phe-/-Phe(NO2)-Arg-Leu =

Synonyms

EC 3.4.23.37, grifolisin, PCP, Pepstatin-insensitive carboxyl proteinase, PSCP, pseudomonalisin, pseudomonapepsin, Pseudomonas serine-carboxyl proteinase, Pseudomonas sp. pepstatin-insensitive carboxyl proteinase, scytalidolisin, scytalidopepsin A, SedA, SedB, SedC, SedD, sedolisin

ECTree

3 Hydrolases

3.4 Acting on peptide bonds (peptidases)

3.4.21 Serine endopeptidases

3.4.21.100 sedolisin

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Results	in table
244	AA Sequence
2	Application
1	CAS Registry Number
2	Cloned(Commentary)
4	Crystallization (Commentary)
1	General Information
26	Inhibitors
7	Ki Value [mM]
57	KM Value [mM]
2	Metals/Ions
10	Molecular Weight [Da]
30	Organism
6	PDB ID
4	pH Optimum
1	pH Range
1	pH Stability
1	pI Value
5	Posttranslational Modification
10	Protein Variants
4	Purification (Commentary)
1	Reaction
1	Reaction Type
21	Reference
5	Source Tissue
2	Specific Activity [micromol/min/mg]
76	Substrates and Products (Substrate)
5	Subunits
27	Synonyms
2	Temperature Optimum [°C]
1	Temperature Range [°C]
2	Temperature Stability [°C]
77	Turnover Number [1/s]

Molecular Weight

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Molecular Weight on EC 3.4.21.100 - sedolisin

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40000

Grifola frondosa

gel filtration

670517

406000

Aspergillus fumigatus

x * 479000, mature sedA, sequence analysis. x * 430000, mature sedB, sequence analysis. x * 406000, mature sedD, sequence analysis. x * 55000, native sedA, SDS-PAGE. x * 60000-90000, native sedB, SDS-PAGE. x * 65000, native sedC, SDS-PAGE. x * 70000-100000, native sedD, SDS-PAGE. x * 46000, sedA and sedB after deglycosylation, SDS-PAGE. x * 48000, sedC and sedD after deglycosylation, SDS-PAGE

663718

42000

Pseudomonas sp.

Pseudomonas sp., gel filtration

137232

43000

Grifola frondosa

1 * 43000, SDS-PAGE

670517

430000

Aspergillus fumigatus

663718

46000

Aspergillus fumigatus

663718

479000

Aspergillus fumigatus

663718

48000

Aspergillus fumigatus

663718

55000

Aspergillus fumigatus

663718

65000

Aspergillus fumigatus

663718