3.4.21.100: sedolisin

This is an abbreviated version!
For detailed information about sedolisin, go to the full flat file.

Word Map on EC 3.4.21.100

3.4.21.100

peptidase

proteinases

subtilisins

merops

ceroid

pcp

lipofuscinosis

tripeptidyl-peptidase

diazoacetyl-dl-norleucine

subtilisin-like

prosegment

tripeptidyl

b-chain

1,2-epoxy-3-p-nitrophenoxypropane

coagulans

medicine

Reaction

Hydrolysis of the B chain of insulin at -Glu13-/-Ala-, -Leu15-/-Tyr- and -Phe25-/-Tyr-, and angiotensin I at -Tyr4-/-Ile-. A good synthetic substrate is Lys-Pro-Ile-Glu-Phe-/-Phe(NO2)-Arg-Leu =

Synonyms

EC 3.4.23.37, grifolisin, PCP, Pepstatin-insensitive carboxyl proteinase, PSCP, pseudomonalisin, pseudomonapepsin, Pseudomonas serine-carboxyl proteinase, Pseudomonas sp. pepstatin-insensitive carboxyl proteinase, scytalidolisin, scytalidopepsin A, SedA, SedB, SedC, SedD, sedolisin

ECTree

3 Hydrolases

3.4 Acting on peptide bonds (peptidases)

3.4.21 Serine endopeptidases

3.4.21.100 sedolisin

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Results	in table
244	AA Sequence
2	Application
1	CAS Registry Number
2	Cloned(Commentary)
4	Crystallization (Commentary)
1	General Information
26	Inhibitors
7	Ki Value [mM]
57	KM Value [mM]
2	Metals/Ions
10	Molecular Weight [Da]
30	Organism
6	PDB ID
4	pH Optimum
1	pH Range
1	pH Stability
1	pI Value
5	Posttranslational Modification
10	Protein Variants
4	Purification (Commentary)
1	Reaction
1	Reaction Type
21	Reference
5	Source Tissue
2	Specific Activity [micromol/min/mg]
76	Substrates and Products (Substrate)
5	Subunits
27	Synonyms
2	Temperature Optimum [°C]
1	Temperature Range [°C]
2	Temperature Stability [°C]
77	Turnover Number [1/s]

Purification

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Purification on EC 3.4.21.100 - sedolisin

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2 entries

recombinant mutant enzymes D124A, D225A, D254A, E217A, D84A, D170A, D328A and E222A

Pseudomonas sp.

P42790

651950

SedB purified by ion-exchange chromatography and gel filtration

Aspergillus fumigatus

663718