Information on EC 3.4.21.100 - sedolisin

Word Map on EC 3.4.21.100
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)


The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY hide
3.4.21.100
-
RECOMMENDED NAME
GeneOntology No.
sedolisin
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
Hydrolysis of the B chain of insulin at -Glu13-/-Ala-, -Leu15-/-Tyr- and -Phe25-/-Tyr-, and angiotensin I at -Tyr4-/-Ile-. A good synthetic substrate is Lys-Pro-Ile-Glu-Phe-/-Phe(NO2)-Arg-Leu
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of peptide bond
-
-
serine endopeptidase
-
CAS REGISTRY NUMBER
COMMENTARY hide
848318-58-1
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strain NCPF 7367
-
-
Manually annotated by BRENDA team
strain NCPF 7367
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
No. T-22
-
-
Manually annotated by BRENDA team
No. T-22
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(7-amido-4-methylcoumaryl)-Lys-Pro-Pro-Leu-Glu-Tyr-Arg-Leu-Gly-Lys(dinitrophenyl)-Gly + H2O
(7-amido-4-methylcoumaryl)-Lys-Pro-Pro-Leu-Glu + Tyr-Arg-Leu-Gly-Lys(dinitrophenyl)-Gly
show the reaction diagram
-
-
-
?
Acid-denatured hemoglobin + H2O
?
show the reaction diagram
Ala-Ala-Phe-p-nitroanilide + H2O
Ala-Ala-Phe + p-nitroaniline
show the reaction diagram
Ala-Pro-Gly-Asp-Arg-Ile-Tyr-Val-His-Pro-Phe + H2O
Arg-Pro-Gly + Asp-Arg-Ile + Tyr-Val-His-Pro-Phe
show the reaction diagram
casein + H2O
?
show the reaction diagram
Hemoglobin + H2O
?
show the reaction diagram
-
-
-
-
?
Leu-Leu-Glu-Phe-Leu + H2O
Leu-Leu-Glu + Phe-Leu
show the reaction diagram
Lys-Pro-Ala-Glu-Phe-p-nitrophenylalanine-Arg-Leu + H2O
Lys-Pro-Ala-Glu-Phe + p-nitrophenylalanine-Arg-Leu
show the reaction diagram
Lys-Pro-Ala-Leu-Phe-p-nitrophenylalanine-Arg-Leu + H2O
?
show the reaction diagram
-
-
?
Lys-Pro-Arg-Glu-Phe-p-nitrophenylalanine-Arg-Leu + H2O
Lys-Pro-Arg-Glu-Phe + p-nitrophenylalanine-Arg-Leu
show the reaction diagram
Lys-Pro-Asn-Glu-Phe-p-nitrophenylalanine-Arg-Leu + H2O
Lys-Pro-Asn-Glu-Phe + p-nitrophenylalanine-Arg-Leu
show the reaction diagram
-
-
-
?
Lys-Pro-Asp-Glu-Phe-p-nitrophenylalanine-Arg-Leu + H2O
Lys-Pro-Asp-Glu-Phe + p-nitrophenylalanine-Arg-Leu
show the reaction diagram
-
-
-
?
Lys-Pro-Gln-Glu-Phe-p-nitrophenylalanine-Arg-Leu + H2O
Lys-Pro-Gln-Glu-Phe + p-nitrophenylalanine-Arg-Leu
show the reaction diagram
-
-
-
?
Lys-Pro-Glu-Glu-Phe-p-nitrophenylalanine-Arg-Leu + H2O
Lys-Pro-Glu-Glu-Phe + p-nitrophenylalanine-Arg-Leu
show the reaction diagram
-
-
-
?
Lys-Pro-Ile-Ala-Phe-p-nitrophenylalanine-Arg-Leu + H2O
Lys-Pro-Ile-Ala-Phe + p-nitrophenylalanine-Arg-Leu
show the reaction diagram
-
-
-
?
Lys-Pro-Ile-Arg-Phe-p-nitrophenylalanine-Arg-Leu + H2O
Lys-Pro-Ile-Arg-Phe + p-nitrophenylalanine-Arg-Leu
show the reaction diagram
-
-
-
?
Lys-Pro-Ile-Asn-Phe-p-nitrophenylalanine-Arg-Leu + H2O
Lys-Pro-Ile-Asn-Phe + p-nitrophenylalanine-Arg-Leu
show the reaction diagram
-
-
-
?
Lys-Pro-Ile-Asp-Phe-p-nitrophenylalanine-Arg-Leu + H2O
Lys-Pro-Ile-Asp-Phe + p-nitrophenylalanine-Arg-Leu
show the reaction diagram
-
-
-
?
Lys-Pro-Ile-Gln-Phe-p-nitrophenylalanine-Arg-Leu + H2O
Lys-Pro-Ile-Gln-Phe + p-nitrophenylalanine-Arg-Leu
show the reaction diagram
-
-
-
?
Lys-Pro-Ile-Glu-Phe-(4-nitro)Phe-Arg-Leu + H2O
Lys-Pro-Ile-Glu-Phe + (4-nitro)Phe-Arg-Leu
show the reaction diagram
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Ala-Leu + H2O
Lys-Pro-Ile-Glu-Phe + p-nitrophenylalanine-Ala-Leu
show the reaction diagram
-
-
-
?
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Ala + H2O
Lys-Pro-Ile-Glu-Phe + p-nitrophenylalanine-Arg-Ala
show the reaction diagram
-
-
-
?
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Arg + H2O
Lys-Pro-Ile-Glu-Phe + p-nitrophenylalanine-Arg-Arg
show the reaction diagram
-
-
-
?
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Asp + H2O
Lys-Pro-Ile-Glu-Phe + p-nitrophenylalanine-Arg-Asp
show the reaction diagram
-
-
-
?
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Ile + H2O
Lys-Pro-Ile-Glu-Phe + p-nitrophenylalanine-Arg-Ile
show the reaction diagram
-
-
-
?
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Leu + H2O
Lys-Pro-Ile-Glu-Phe + p-nitrophenylalanine-Arg-Leu
show the reaction diagram
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Phe + H2O
Lys-Pro-Ile-Glu-Phe + p-nitrophenylalanine-Arg-Phe
show the reaction diagram
-
-
-
?
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Ser + H2O
Lys-Pro-Ile-Glu-Phe + p-nitrophenylalanine-Arg-Ser
show the reaction diagram
-
-
-
?
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Tyr + H2O
Lys-Pro-Ile-Glu-Phe + p-nitrophenylalanine-Arg-Tyr
show the reaction diagram
-
-
-
?
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Val + H2O
Lys-Pro-Ile-Glu-Phe + p-nitrophenylalanine-Arg-Val
show the reaction diagram
-
-
-
?
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Asn-Leu + H2O
Lys-Pro-Ile-Glu-Phe + p-nitrophenylalanine-Asn-Leu
show the reaction diagram
-
-
-
?
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Asp-Leu + H2O
Lys-Pro-Ile-Glu-Phe + p-nitrophenylalanine-Asp-Leu
show the reaction diagram
-
-
-
?
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Glu-Leu + H2O
Lys-Pro-Ile-Glu-Phe + p-nitrophenylalanine-Glu-Leu
show the reaction diagram
-
-
-
?
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Ile-Leu + H2O
Lys-Pro-Ile-Glu-Phe + p-nitrophenylalanine-Ile-Leu
show the reaction diagram
-
-
-
?
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Leu-Leu + H2O
Lys-Pro-Ile-Glu-Phe + p-nitrophenylalanine-Leu-Leu
show the reaction diagram
-
-
-
?
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Lys-Leu + H2O
Lys-Pro-Ile-Glu-Phe + p-nitrophenylalanine-Lys-Leu
show the reaction diagram
-
-
-
?
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Ser-Leu + H2O
Lys-Pro-Ile-Glu-Phe + p-nitrophenylalanine-Ser-Leu
show the reaction diagram
-
-
-
?
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Val-Leu + H2O
Lys-Pro-Ile-Glu-Phe + p-nitrophenylalanine-Val-Leu
show the reaction diagram
-
-
-
?
Lys-Pro-Ile-His-Phe-p-nitrophenylalanine-Arg-Leu + H2O
Lys-Pro-Ile-His-Phe + p-nitrophenylalanine-Arg-Leu
show the reaction diagram
-
-
-
?
Lys-Pro-Ile-Ile-Phe-p-nitrophenylalanine-Arg-Leu + H2O
Lys-Pro-Ile-Ile-Phe + p-nitrophenylalanine-Arg-Leu
show the reaction diagram
-
-
-
?
Lys-Pro-Ile-Leu-Phe-p-nitrophenylalanine-Arg-Leu + H2O
Lys-Pro-Ile-Leu-Phe + p-nitrophenylalanine-Arg-Leu
show the reaction diagram
-
-
-
?
Lys-Pro-Ile-Lys-Phe-p-nitrophenylalanine-Arg-Leu + H2O
Lys-Pro-Ile-Lys-Phe + p-nitrophenylalanine-Arg-Leu
show the reaction diagram
-
-
-
?
Lys-Pro-Ile-Nle-Phe-p-nitrophenylalanine-Arg-Leu + H2O
Lys-Pro-Ile-Nle-Phe + p-nitrophenylalanine-Arg-Leu
show the reaction diagram
-
-
-
?
Lys-Pro-Ile-Pro-Phe-p-nitrophenylalanine-Arg-Leu + H2O
Lys-Pro-Ile-Pro-Phe + p-nitrophenylalanine-Arg-Leu
show the reaction diagram
-
-
-
?
Lys-Pro-Ile-Ser-Phe-p-nitrophenylalanine-Arg-Leu + H2O
Lys-Pro-Ile-Ser-Phe + p-nitrophenylalanine-Arg-Leu
show the reaction diagram
-
-
-
?
Lys-Pro-Ile-Thr-Phe-p-nitrophenylalanine-Arg-Leu + H2O
Lys-Pro-Ile-Thr-Phe + p-nitrophenylalanine-Arg-Leu
show the reaction diagram
-
-
-
?
Lys-Pro-Ile-Val-Phe-p-nitrophenylalanine-Arg-Leu + H2O
Lys-Pro-Ile-Val-Phe + p-nitrophenylalanine-Arg-Leu
show the reaction diagram
-
-
-
?
Lys-Pro-Leu-Glu-Phe-p-nitrophenylalanine-Arg-Leu + H2O
Lys-Pro-Leu-Glu-Phe + p-nitrophenylalanine-Arg-Leu
show the reaction diagram
-
-
-
?
Lys-Pro-Lys-Glu-Phe-p-nitrophenylalanine-Arg-Leu + H2O
Lys-Pro-Lys-Glu-Phe + p-nitrophenylalanine-Arg-Leu
show the reaction diagram
-
-
-
?
Lys-Pro-Ser-Glu-Phe-p-nitrophenylalanine-Arg-Leu + H2O
Lys-Pro-Ser-Glu-Phe + p-nitrophenylalanine-Arg-Leu
show the reaction diagram
Lys-Pro-Thr-Glu-Phe-p-nitrophenylalanine-Arg-Leu + H2O
Lys-Pro-Thr-Glu-Phe + p-nitrophenylalanine-Arg-Leu
show the reaction diagram
-
-
-
?
Lys-Pro-Val-Glu-Phe-p-nitrophenylalanine-Arg-Leu + H2O
Lys-Pro-Val-Glu-Phe + p-nitrophenylalanine-Arg-Leu
show the reaction diagram
-
-
-
?
Milk casein + H2O
?
show the reaction diagram
-
-
-
-
?
Oxidized insulin B-chain + H2O
?
show the reaction diagram
-
hydrolysis of four bonds, F1-V2, A14-L15, G20-E21, F24-F25
-
-
?
Phe-Pro-Ala-p-nitroanilide + H2O
Phe-Pro-Ala + p-nitroaniline
show the reaction diagram
Ser-Pro-Ala-Lys-Phe-(4-nitro)Phe-Arg-Leu + H2O
Ser-Pro-Ala-Lys-Phe + (4-nitro)Phe-Arg-Leu
show the reaction diagram
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,2-epoxy-3-(4-nitrophenoxy)propane
-
modifies the enzyme with concomitant loss of activity
acetyl-Ile-Ala-Phe
-
-
acetyl-Ile-Pro-Phe
-
-
chymostatin
-
-
HgCl2
-
slight
iodotyrosine
-
-
iodotyrostatin
-
-
N,N'-dicyclohexylcarbodiimide
-
inhibitor binds to Asp140 and Glu222 of the enzyme, inactivation with pseudo-first-order kinetics, inactivation is prevented by a competitive inhibitor, tyrostatin
PCMB
-
slight
pseudo-iodotyrostatin
pseudo-tyrostatin
-
-
pseudoiodotyrostatin
-
-
TPCK
-
0.1 mM, 14% inhibition
Tyrostatin
additional information
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00012
(7-amido-4-methylcoumaryl)-Lys-Pro-Pro-Leu-Glu-Tyr-Arg-Leu-Gly-Lys(dinitrophenyl)-Gly
-
-
0.013
Lys-Pro-Ala-Asp-Phe-(4-nitro)Phe-Arg-Leu
-
-
0.0174
Lys-Pro-Ala-Glu-Phe-p-nitrophenylalanine-Arg-Leu
-
pH 3.5, 37C
0.0067 - 0.149
Lys-Pro-Ala-Leu-Phe-p-nitrophenylalanine-Arg-Leu
0.06
Lys-Pro-Ala-Lys-Leu-(4-nitro)Phe-Arg-Leu
-
-
0.095
Lys-Pro-Ala-Lys-Phe-(4-nitro)Phe-Arg-Leu
-
-
0.0224
Lys-Pro-Arg-Glu-Phe-p-nitrophenylalanine-Arg-Leu
-
pH 3.5, 37C
0.0247
Lys-Pro-Asn-Glu-Phe-p-nitrophenylalanine-Arg-Leu
-
pH 3.5, 37C
0.0242
Lys-Pro-Asp-Glu-Phe-p-nitrophenylalanine-Arg-Leu
-
pH 3.5, 37C
0.0162
Lys-Pro-Gln-Glu-Phe-p-nitrophenylalanine-Arg-Leu
-
pH 3.5, 37C
0.00612
Lys-Pro-Glu-Glu-Phe-p-nitrophenylalanine-Arg-Leu
-
pH 3.5, 37C
0.0131
Lys-Pro-Ile-Ala-Phe-p-nitrophenylalanine-Arg-Leu
-
pH 3.5, 37C
0.01
Lys-Pro-Ile-Arg-Phe-p-nitrophenylalanine-Arg-Leu
-
pH 3.5, 37C
0.0189
Lys-Pro-Ile-Asn-Phe-p-nitrophenylalanine-Arg-Leu
-
pH 3.5, 37C
0.0106
Lys-Pro-Ile-Asp-Phe-p-nitrophenylalanine-Arg-Leu
-
pH 3.5, 37C
0.0207
Lys-Pro-Ile-Gln-Phe-p-nitrophenylalanine-Arg-Leu
-
pH 3.5, 37C
0.003 - 0.052
Lys-Pro-Ile-Glu-Phe-(4-nitro)Phe-Arg-Leu
0.00827
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Ala-Leu
-
pH 3.5, 37C
0.00697
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Ala
-
pH 3.5, 37C
0.00542
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Arg
-
pH 3.5, 37C
0.00962
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Asp
-
pH 3.5, 37C
0.0037
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Ile
-
pH 3.5, 37C
0.0111
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Leu
-
pH 3.5, 37C
0.0074
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Phe
-
pH 3.5, 37C
0.0101
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Ser
-
pH 3.5, 37C
0.00627
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Tyr
-
pH 3.5, 37C
0.00547
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Val
-
pH 3.5, 37C
0.0309
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Asn-Leu
-
pH 3.5, 37C
0.00946
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Asp-Leu
-
pH 3.5, 37C
0.00901
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Glu-Leu
-
pH 3.5, 37C
0.0153
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Ile-Leu
-
pH 3.5, 37C
0.00567
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Leu-Leu
-
pH 3.5, 37C
0.0189
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Lys-Leu
-
pH 3.5, 37C
0.014
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Ser-Leu
-
pH 3.5, 37C
0.028
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Val-Leu
-
pH 3.5, 37C
0.0229
Lys-Pro-Ile-His-Phe-p-nitrophenylalanine-Arg-Leu
-
pH 3.5, 37C
0.017
Lys-Pro-Ile-Ile-Phe-p-nitrophenylalanine-Arg-Leu
-
pH 3.5, 37C
0.00422
Lys-Pro-Ile-Leu-Phe-p-nitrophenylalanine-Arg-Leu
-
pH 3.5, 37C
0.0564
Lys-Pro-Ile-Lys-Phe-p-nitrophenylalanine-Arg-Leu
-
pH 3.5, 37C
0.00713
Lys-Pro-Ile-Nle-Phe-p-nitrophenylalanine-Arg-Leu
-
pH 3.5, 37C
0.0275
Lys-Pro-Ile-Pro-Phe-p-nitrophenylalanine-Arg-Leu
-
pH 3.5, 37C
0.00999
Lys-Pro-Ile-Ser-Phe-p-nitrophenylalanine-Arg-Leu
-
pH 3.5, 37C
0.0106
Lys-Pro-Ile-Thr-Phe-p-nitrophenylalanine-Arg-Leu
-
pH 3.5, 37C
0.00782
Lys-Pro-Ile-Val-Phe-p-nitrophenylalanine-Arg-Leu
-
pH 3.5, 37C
0.00884
Lys-Pro-Leu-Glu-Phe-p-nitrophenylalanine-Arg-Leu
-
pH 3.5, 37C
0.0275
Lys-Pro-Lys-Glu-Phe-p-nitrophenylalanine-Arg-Leu
-
pH 3.5, 37C
0.00875
Lys-Pro-Ser-Glu-Phe-p-nitrophenylalanine-Arg-Leu
-
pH 3.5, 37C
0.0132
Lys-Pro-Thr-Glu-Phe-p-nitrophenylalanine-Arg-Leu
-
pH 3.5, 37C
0.0115
Lys-Pro-Val-Glu-Phe-p-nitrophenylalanine-Arg-Leu
-
pH 3.5, 37C
0.019
Lys-Pro-Val-Ser-Tyr-(4-nitro)Phe-Arg-Leu
-
-
additional information
additional information
-
overview: Km of synthetic substrates
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
73
(7-amido-4-methylcoumaryl)-Lys-Pro-Pro-Leu-Glu-Tyr-Arg-Leu-Gly-Lys(dinitrophenyl)-Gly
Pseudomonas sp.
-
-
6.8
Lys-Pro-Ala-Asp-Phe-(4-nitro)Phe-Arg-Leu
Pseudomonas sp.
-
-
6 - 57.6
Lys-Pro-Ala-Glu-Phe-p-nitrophenylalanine-Arg-Leu
0.055 - 65.2
Lys-Pro-Ala-Leu-Phe-p-nitrophenylalanine-Arg-Leu
14.7
Lys-Pro-Ala-Lys-Phe-(4-nitro)Phe-Arg-Leu
Pseudomonas sp.
-
-
53.1
Lys-Pro-Arg-Glu-Phe-p-nitrophenylalanine-Arg-Leu
Pseudomonas sp.
-
pH 3.5, 37C
5.5
Lys-Pro-Asn-Gln-Phe-(4-nitro)Phe-Arg-Leu
Pseudomonas sp.
-
-
47.1
Lys-Pro-Asn-Glu-Phe-p-nitrophenylalanine-Arg-Leu
Pseudomonas sp.
-
pH 3.5, 37C
0.052 - 9.68
Lys-Pro-Asp-Glu-Phe-p-nitrophenylalanine-Arg-Leu
24.5
Lys-Pro-Gln-Glu-Phe-p-nitrophenylalanine-Arg-Leu
Pseudomonas sp.
-
pH 3.5, 37C
3.13 - 3.82
Lys-Pro-Glu-Glu-Phe-p-nitrophenylalanine-Arg-Leu
27.7
Lys-Pro-Ile-Ala-Phe-p-nitrophenylalanine-Arg-Leu
Pseudomonas sp.
-
pH 3.5, 37C
12.5
Lys-Pro-Ile-Arg-Phe-p-nitrophenylalanine-Arg-Leu
Pseudomonas sp.
-
pH 3.5, 37C
0.04 - 8.79
Lys-Pro-Ile-Asn-Phe-p-nitrophenylalanine-Arg-Leu
20.9
Lys-Pro-Ile-Asp-Phe-p-nitrophenylalanine-Arg-Leu
Pseudomonas sp.
-
pH 3.5, 37C
10.4
Lys-Pro-Ile-Gln-Phe-p-nitrophenylalanine-Arg-Leu
Pseudomonas sp.
-
pH 3.5, 37C
6.9
Lys-Pro-Ile-Glu-Phe-(4-nitro)Phe-Arg-Leu
Pseudomonas sp.
-
-
19
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Ala-Leu
Pseudomonas sp.
-
pH 3.5, 37C
15.5
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Ala
Pseudomonas sp.
-
pH 3.5, 37C
3.13 - 3.64
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Arg
40.7
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Asp
Pseudomonas sp.
-
pH 3.5, 37C
10.9
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Ile
Pseudomonas sp.
-
pH 3.5, 37C
3.7 - 32.4
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Leu
10.7
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Phe
Pseudomonas sp.
-
pH 3.5, 37C
44.4
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Ser
Pseudomonas sp.
-
pH 3.5, 37C
0.052 - 9.87
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Tyr
11.6
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Val
Pseudomonas sp.
-
pH 3.5, 37C
0.5 - 12.9
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Asn-Leu
33
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Asp-Leu
Pseudomonas sp.
-
pH 3.5, 37C
0.017 - 33.3
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Glu-Leu
3.3 - 5.24
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Ile-Leu
0.031 - 8.38
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Leu-Leu
0.64 - 54.1
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Lys-Leu
11.8
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Ser-Leu
Pseudomonas sp.
-
pH 3.5, 37C
3.3 - 5.38
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Val-Leu
2.93 - 6
Lys-Pro-Ile-His-Phe-p-nitrophenylalanine-Arg-Leu
18.3
Lys-Pro-Ile-Ile-Phe-p-nitrophenylalanine-Arg-Leu
Pseudomonas sp.
-
pH 3.5, 37C
27.2
Lys-Pro-Ile-Leu-Phe-p-nitrophenylalanine-Arg-Leu
Pseudomonas sp.
-
pH 3.5, 37C
9.75
Lys-Pro-Ile-Lys-Phe-p-nitrophenylalanine-Arg-Leu
Pseudomonas sp.
-
pH 3.5, 37C
0.55 - 14.8
Lys-Pro-Ile-Nle-Phe-p-nitrophenylalanine-Arg-Leu
29.3
Lys-Pro-Ile-Pro-Phe-p-nitrophenylalanine-Arg-Leu
Pseudomonas sp.
-
pH 3.5, 37C
0.55 - 14.8
Lys-Pro-Ile-Ser-Phe-p-nitrophenylalanine-Arg-Leu
22.9
Lys-Pro-Ile-Thr-Phe-p-nitrophenylalanine-Arg-Leu
Pseudomonas sp.
-
pH 3.5, 37C
15.7
Lys-Pro-Ile-Val-Phe-p-nitrophenylalanine-Arg-Leu
Pseudomonas sp.
-
pH 3.5, 37C
3.1
Lys-Pro-Leu-Glu-Phe-p-nitrophenylalanine-Arg-Leu
Pseudomonas sp.
-
pH 3.5, 37C
1.5 - 56.3
Lys-Pro-Lys-Glu-Phe-p-nitrophenylalanine-Arg-Leu
20.4
Lys-Pro-Ser-Glu-Phe-p-nitrophenylalanine-Arg-Leu
Pseudomonas sp.
-
pH 3.5, 37C
0.031 - 7.92
Lys-Pro-Thr-Glu-Phe-p-nitrophenylalanine-Arg-Leu
0.55 - 14.8
Lys-Pro-Val-Glu-Phe-p-nitrophenylalanine-Arg-Leu
0.0867
Lys-Pro-Val-Ser-Tyr-(4-nitro)Phe-Arg-Leu
Pseudomonas sp.
-
-
35
Phe-Pro-Ala-p-nitroanilide
Aspergillus fumigatus
-
-
additional information
additional information
Pseudomonas sp.
-
overview: turnover number of synthetic substrates
-
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0006
acetyl-Ile-Ala-Phe
-
-
0.000415
acetyl-Ile-Pro-Phe
-
-
0.000045
chymostatin
-
-
0.000032
iodotyrostatin
-
-
0.000014
pseudo-iodotyrostatin
-
-
0.000056
pseudo-tyrostatin
-
-
0.000006
Tyrostatin
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4920
-
pH 2.8, 40C
additional information
-
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
2.8
-
substrate milk casein
additional information
-
SedB and SedC have an optimum at pH 6.0, while SedD has an optimum peak at pH 5.0
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
1.5 - 7
-
SedB and SedC are active between pH 3.0 and 7.0, while SedD is active between pH 1.5 and 6.0
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
60
-
loss of activity
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.6
-
isoelectric focusing
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
additional information
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
40000
-
gel filtration
42000
-
Pseudomonas sp., gel filtration
43000
-
1 * 43000, SDS-PAGE
46000
-
x * 479000, mature sedA, sequence analysis. x * 430000, mature sedB, sequence analysis. x * 406000, mature sedD, sequence analysis. x * 55000, native sedA, SDS-PAGE. x * 60000-90000, native sedB, SDS-PAGE. x * 65000, native sedC, SDS-PAGE. x * 70000-100000, native sedD, SDS-PAGE. x * 46000, sedA and sedB after deglycosylation, SDS-PAGE. x * 48000, sedC and sedD after deglycosylation, SDS-PAGE
48000
-
x * 479000, mature sedA, sequence analysis. x * 430000, mature sedB, sequence analysis. x * 406000, mature sedD, sequence analysis. x * 55000, native sedA, SDS-PAGE. x * 60000-90000, native sedB, SDS-PAGE. x * 65000, native sedC, SDS-PAGE. x * 70000-100000, native sedD, SDS-PAGE. x * 46000, sedA and sedB after deglycosylation, SDS-PAGE. x * 48000, sedC and sedD after deglycosylation, SDS-PAGE
55000
-
x * 479000, mature sedA, sequence analysis. x * 430000, mature sedB, sequence analysis. x * 406000, mature sedD, sequence analysis. x * 55000, native sedA, SDS-PAGE. x * 60000-90000, native sedB, SDS-PAGE. x * 65000, native sedC, SDS-PAGE. x * 70000-100000, native sedD, SDS-PAGE. x * 46000, sedA and sedB after deglycosylation, SDS-PAGE. x * 48000, sedC and sedD after deglycosylation, SDS-PAGE
65000
-
x * 479000, mature sedA, sequence analysis. x * 430000, mature sedB, sequence analysis. x * 406000, mature sedD, sequence analysis. x * 55000, native sedA, SDS-PAGE. x * 60000-90000, native sedB, SDS-PAGE. x * 65000, native sedC, SDS-PAGE. x * 70000-100000, native sedD, SDS-PAGE. x * 46000, sedA and sedB after deglycosylation, SDS-PAGE. x * 48000, sedC and sedD after deglycosylation, SDS-PAGE
406000
-
x * 479000, mature sedA, sequence analysis. x * 430000, mature sedB, sequence analysis. x * 406000, mature sedD, sequence analysis. x * 55000, native sedA, SDS-PAGE. x * 60000-90000, native sedB, SDS-PAGE. x * 65000, native sedC, SDS-PAGE. x * 70000-100000, native sedD, SDS-PAGE. x * 46000, sedA and sedB after deglycosylation, SDS-PAGE. x * 48000, sedC and sedD after deglycosylation, SDS-PAGE
430000
-
x * 479000, mature sedA, sequence analysis. x * 430000, mature sedB, sequence analysis. x * 406000, mature sedD, sequence analysis. x * 55000, native sedA, SDS-PAGE. x * 60000-90000, native sedB, SDS-PAGE. x * 65000, native sedC, SDS-PAGE. x * 70000-100000, native sedD, SDS-PAGE. x * 46000, sedA and sedB after deglycosylation, SDS-PAGE. x * 48000, sedC and sedD after deglycosylation, SDS-PAGE
479000
-
x * 479000, mature sedA, sequence analysis. x * 430000, mature sedB, sequence analysis. x * 406000, mature sedD, sequence analysis. x * 55000, native sedA, SDS-PAGE. x * 60000-90000, native sedB, SDS-PAGE. x * 65000, native sedC, SDS-PAGE. x * 70000-100000, native sedD, SDS-PAGE. x * 46000, sedA and sedB after deglycosylation, SDS-PAGE. x * 48000, sedC and sedD after deglycosylation, SDS-PAGE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glycoprotein
-
potential asparagine-linked glycosylation at posititons 231 and 487, preproprotein numbering
proteolytic modification
additional information
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
crystal structure complexed with a number of inhibitors (tyrostatin, pseudo-tyrostatin, acetyl-Ile-Pro-Phe, acetyl-Ile-Ala-Phe, iodotyrostatin, pseudotyrostatin and chymostatin), have been solved and refined at high-level to atomic-level resolution
-
crystal structure solved by single-wavelength anomalous diffraction using the absorption peak of bromide anions. Structures of the uninhibited enzyme and of complexes with iodotyrosine are refined at 1.0-1.4 A resolution
-
high-resolution crystrallographic analysis of a complex of the enzyme with pseudo-iodotyrostatin
-
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4 - 5.5
-
15 h, 37C, stable
137232
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
45
-
15 min, stable below
65
-
complete loss of activity after 15 min
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
recombinant mutant enzymes D124A, D225A, D254A, E217A, D84A, D170A, D328A and E222A
-
SedB purified by ion-exchange chromatography and gel filtration
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
-
SedA and SedC cloned into vector pUC18. Heterologously expressed in Pichia pastoris. SedA, SedB, SedC and SedD cloned into vector pET-11aH6 and expressed in Escherichia coli BL21
-
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D124A
-
slightly lower proteinase activity than that of wild-type enzyme
D170A
-
mutant enzyme is not processed autocatalytically in Escherichia coli
D225A
-
mutant enzyme shows almost the same proteinase activity as wild-type enzyme
D254A
-
mutant enzyme shows almost the same proteinase activity as wild-type enzyme
D328A
-
mutant enzyme is not processed autocatalytically in Escherichia coli
D84A
-
proteinase activity is 0.2% of that of wild-type enzyme, mutant enzyme is very unstable
E217A
-
mutant enzyme shows almost the same proteinase activity as wild-type enzyme
E222A
-
mutant enzyme is not processed autocatalytically in Escherichia coli
additional information
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine
Show AA Sequence (130 entries)
Please use the Sequence Search for a specific query.