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Literature summary for 3.4.21.100 extracted from

  • Suzuki, N.; Nishibori, K.; Oodaira, Y.; Kitamura, S.; Michigami, K.; Nagata, K.; Tatara, Y.; Lee, B.R.; Ichishima, E.
    Grifolisin, a member of the sedolisin family produced by the fungus Grifola frondosa (2005), Phytochemistry, 66, 983-990.
    View publication on PubMed
Search for more literature for 3.4.21.100

Inhibitors

Inhibitors Comment Organism Structure
additional information not inhibitory: pepstatin, 1,10-phenanthroline, EDTA, N-ethylmaleimide Grifola frondosa
TPCK 0.1 mM, 14% inhibition Grifola frondosa
Tyrostatin 0.5 mM, 15% inhibition Grifola frondosa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
 gel filtration Grifola frondosa
43000
-
 1 * 43000, SDS-PAGE Grifola frondosa

Organism

Organism UniProt Comment Textmining
Grifola frondosa
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein potential asparagine-linked glycosylation at posititons 231 and 487, preproprotein numbering Grifola frondosa
proteolytic modification sequence contains a prepropeptide of 235 amino acids, mature forms consists of 365 amino acids Grifola frondosa

Source Tissue

Source Tissue Comment Organism Textmining
fruit body
-
 Grifola frondosa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4920
-
 pH 2.8, 40°C Grifola frondosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Hemoglobin + H2O
-
 Grifola frondosa ?
-
 ?
Milk casein + H2O
-
 Grifola frondosa ?
-
 ?
Oxidized insulin B-chain + H2O hydrolysis of four bonds, F1-V2, A14-L15, G20-E21, F24-F25 Grifola frondosa ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 43000, SDS-PAGE Grifola frondosa

Synonyms

Synonyms Comment Organism
grifolisin
-
 Grifola frondosa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Grifola frondosa

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
60
-
 loss of activity Grifola frondosa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
2.8
-
 substrate milk casein Grifola frondosa
3
-
 substrate hemoglobin Grifola frondosa

pI Value

Organism Comment pI Value Maximum pI Value
Grifola frondosa isoelectric focusing
-
 4.6