3.4.21.100: sedolisin

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For detailed information about sedolisin, go to the full flat file.

Reaction

Hydrolysis of the B chain of insulin at -Glu13-/-Ala-, -Leu15-/-Tyr- and -Phe25-/-Tyr-, and angiotensin I at -Tyr4-/-Ile-. A good synthetic substrate is Lys-Pro-Ile-Glu-Phe-/-Phe(NO2)-Arg-Leu =

Synonyms

EC 3.4.23.37, grifolisin, PCP, Pepstatin-insensitive carboxyl proteinase, PSCP, pseudomonalisin, pseudomonapepsin, Pseudomonas serine-carboxyl proteinase, Pseudomonas sp. pepstatin-insensitive carboxyl proteinase, scytalidolisin, scytalidopepsin A, SedA, SedB, SedC, SedD, sedolisin

ECTree

     3 Hydrolases

         3.4 Acting on peptide bonds (peptidases)

             3.4.21 Serine endopeptidases

                3.4.21.100 sedolisin

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Results	in table
244	AA Sequence
2	Application
1	CAS Registry Number
2	Cloned(Commentary)
4	Crystallization (Commentary)
1	General Information
26	Inhibitors
7	Ki Value [mM]
57	KM Value [mM]
2	Metals/Ions
10	Molecular Weight [Da]
30	Organism
6	PDB ID
4	pH Optimum
1	pH Range
1	pH Stability
1	pI Value
5	Posttranslational Modification
10	Protein Variants
4	Purification (Commentary)
1	Reaction
1	Reaction Type
21	Reference
5	Source Tissue
2	Specific Activity [micromol/min/mg]
76	Substrates and Products (Substrate)
5	Subunits
27	Synonyms
2	Temperature Optimum [°C]
1	Temperature Range [°C]
2	Temperature Stability [°C]
77	Turnover Number [1/s]

Specific Activity

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Specific Activity on EC 3.4.21.100 - sedolisin

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SPECIFIC ACTIVITY [µmol/min/mg]

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

4920

Grifola frondosa

-

pH 2.8, 40°C

670517

additional information

Pseudomonas sp.

-

-

137232