3.4.13.18: cytosol nonspecific dipeptidase

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For detailed information about cytosol nonspecific dipeptidase, go to the full flat file.

Reaction

hydrolysis of dipeptides, preferentially hydrophobic dipeptides including prolyl amino acids =

Synonyms

carboxypeptidase of glutamate like-B, CG17337, CNDP2, CPGL, CPGL-B, diglycinase, dipeptidase, glycylglycine, DPP8, DPP8-v3, EC 3.4.13.1, EC 3.4.13.11, EC 3.4.13.13, EC 3.4.13.15, EC 3.4.13.2, EC 3.4.13.3, EC 3.4.13.8, EC 3.4.3.1, EC 3.4.3.2, EC 3.4.3.3, EC 3.4.3.6, Gly-Leu hydrolase, glycyl-glycine dipeptidase, glycyl-L-leucine dipeptidase, glycyl-L-leucine hydrolase, glycyl-L-leucine peptidase, glycyl-leucine dipeptidase, glycylleucine dipeptidase, glycylleucine dipeptide hydrolase, glycylleucine hydrolase, glycylleucine peptidase, human cytosolic non-specific dipeptidase, iminodipeptidase, L-amino-acyl-L-amino-acid hydrolase, L-prolylglycine dipeptidase, N2-beta-alanylarginine dipeptidase, non-specific dipeptidase, PepD, Peptidase A, Pro-X dipeptidase, Pro-Xaa dipeptidase, prolinase, prolyl dipeptidase

ECTree

     3 Hydrolases

         3.4 Acting on peptide bonds (peptidases)

             3.4.13 Dipeptidases

                3.4.13.18 cytosol nonspecific dipeptidase

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Results	in table
10	Activating Compound
1878	AA Sequence
2	CAS Registry Number
6	Cloned(Commentary)
132	Disease/ Diagnostics
1	General Information
14	General Stability
14	IC50 Value
89	Inhibitors
99	KM Value [mM]
13	Localization
24	Metals/Ions
25	Molecular Weight [Da]
1	Natural Substrates/ Products (Substrates)
228	Organism
7	Pathway
6	PDB ID
17	pH Optimum
1	pH Range
9	pH Stability
1	pI Value
4	Protein Variants
22	Purification (Commentary)
1	Reaction
1	Reaction Type
52	Reference
74	Source Tissue
14	Specific Activity [micromol/min/mg]
6	Storage Stability
211	Substrates and Products (Substrate)
11	Subunits
55	Synonyms
4	Temperature Optimum [°C]
8	Temperature Stability [°C]
41	Turnover Number [1/s]

General Stability

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General Stability on EC 3.4.13.18 - cytosol nonspecific dipeptidase

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GENERAL STABILITY

ORGANISM

UNIPROT

LITERATURE

addition of 2 mg/ml bovine serum albumin stabilizes the enzyme for assay for 5 min at 40°C

Mus musculus

-

137174

dialysis against distilled water causes high degree of inactivation

Saccharomyces cerevisiae

-

137182

dilute solutions, 0.1 mg/ml in Tris buffer, pH 8.1, of the pure enzyme lose 20% of their activity on standing for one week at 4°C or 2 h at 40°C

Saccharomyces cerevisiae

-

137182

DTT stabilizes

2 entries

extremely unstable, even in presence of DTT

Platyrrhini

-

137189

freezing and thawing without addition of glycerol causes 50% loss of activity

Saccharomyces cerevisiae

-

137182

Mn2+ stabilizes

Sus scrofa

-

137176

stabilized to only a limited extent in the presence of 50%-saturated (NH4)2SO4 or 12.5% v/v glycerol

Platyrrhini

-

137189

stable to 18 h dialysis, at 10�C against 0.02 M phosphate buffer, pH 7.3-8.3

2 entries

sucrose is necessary for stability

Mus musculus

-

137174

very unstable in the absence of Mn2+ or Mg2+

Mycolicibacterium phlei

-

137181

DTT stabilizes

Homo sapiens

-

137175

DTT stabilizes

Sus scrofa

-

137176

stable to 18 h dialysis, at 10�C against 0.02 M phosphate buffer, pH 7.3-8.3

Mus musculus

-

137178

stable to 18 h dialysis, at 10�C against 0.02 M phosphate buffer, pH 7.3-8.3

Escherichia coli

-

137173, 137178