General Stability | Organism |
---|---|
dialysis against distilled water causes high degree of inactivation | Saccharomyces cerevisiae |
dilute solutions, 0.1 mg/ml in Tris buffer, pH 8.1, of the pure enzyme lose 20% of their activity on standing for one week at 4°C or 2 h at 40°C | Saccharomyces cerevisiae |
freezing and thawing without addition of glycerol causes 50% loss of activity | Saccharomyces cerevisiae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | complex pattern of inhibition or activation with different dipeptide substrates | Saccharomyces cerevisiae | |
Mn2+ | complex pattern of inhibition or activation with different dipeptide substrates | Saccharomyces cerevisiae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
4 * 35000, SDS-PAGE | Saccharomyces cerevisiae |
130000 | - |
gel filtration | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Saccharomyces cerevisiae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Saccharomyces cerevisiae |
Storage Stability | Organism |
---|---|
frozen, in presence of 25% v/v glycerol or 0.2 M sucrose, stable for months | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Ala-Ile + H2O | - |
Saccharomyces cerevisiae | Ala + Ile | - |
? | |
additional information | substrates must contain uncharged side chains and free alpha-amino and carboxyl groups | Saccharomyces cerevisiae | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 35000, SDS-PAGE | Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
pI: 6.3 | Saccharomyces cerevisiae |
7.8 | 8.3 | - |
Saccharomyces cerevisiae |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 8.3 | about 35% of activity maximum at pH 6,5, activity maximum at pH 7.8-8.3 | Saccharomyces cerevisiae |