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1.42
Ala-Ala-p-nitroanilide
-
-
0.12 - 2.72
Ala-Pro-p-nitroanilide
0.027 - 1.673
Arg-Pro-p-nitroanilide
2.05 - 2.668
Asp-Pro-p-nitroanilide
29
Gly-L-Asn
-
tumor enzyme
0.48 - 4.599
Gly-Pro-p-nitroanilide
0.023 - 0.39
Leu-Pro-p-nitroanilide
0.016 - 1.358
Lys-Pro-p-nitroanilide
0.029 - 0.988
Met-Pro-p-nitroanilide
0.47
Pro-Ala
-
at pH 8.0 and 9.2
0.238 - 7.462
Ser-Pro-p-nitroanilide
0.28
Ala-Leu
-
tumor enzyme
0.44
Ala-Leu
-
Tris buffer
1
Ala-Leu
-
phosphate buffer
0.12
Ala-Pro-p-nitroanilide
-
-
0.127
Ala-Pro-p-nitroanilide
-
wild type enzyme
1.495
Ala-Pro-p-nitroanilide
-
mutant enzyme F822A
2.219
Ala-Pro-p-nitroanilide
-
mutant enzyme V833A
2.397
Ala-Pro-p-nitroanilide
-
mutant enzyme Y844A
2.72
Ala-Pro-p-nitroanilide
-
mutant enzyme H859A
0.027
Arg-Pro-p-nitroanilide
-
wild type enzyme
0.27
Arg-Pro-p-nitroanilide
-
mutant enzyme H859A
0.31
Arg-Pro-p-nitroanilide
-
-
1.673
Arg-Pro-p-nitroanilide
-
mutant enzyme F822A
2.05
Asp-Pro-p-nitroanilide
-
-
2.668
Asp-Pro-p-nitroanilide
-
wild type enzyme
4.4
Gly-Ile
-
Tris buffer
10
Gly-Ile
-
phosphate buffer
3.16
Gly-L-Leu
-
-
0.79
Gly-Leu
-
at pH 8.0 and 9.2
2.74
Gly-Leu
-
Tris buffer
10
Gly-Leu
-
phosphate buffer
2.4
Gly-Phe
-
Tris buffer
10
Gly-Phe
-
phosphate buffer
0.48
Gly-Pro-p-nitroanilide
-
-
0.528
Gly-Pro-p-nitroanilide
-
wild type enzyme
2.731
Gly-Pro-p-nitroanilide
-
mutant enzyme H859A
4.599
Gly-Pro-p-nitroanilide
-
mutant enzyme F822A
3.6
Gly-Val
-
Tris buffer
8
Gly-Val
-
phosphate buffer
0.33
Leu-Gly
-
tumor enzyme
0.25
Leu-Leu
-
tumor enzyme
0.25
Leu-Leu
-
tumor enzyme
0.023
Leu-Pro-p-nitroanilide
-
wild type enzyme
0.348
Leu-Pro-p-nitroanilide
-
mutant enzyme H859A
0.39
Leu-Pro-p-nitroanilide
-
mutant enzyme F822A
0.016
Lys-Pro-p-nitroanilide
-
wild type enzyme
0.507
Lys-Pro-p-nitroanilide
-
mutant enzyme H859A
1.358
Lys-Pro-p-nitroanilide
-
mutant enzyme F822A
0.029
Met-Pro-p-nitroanilide
-
wild type enzyme
0.325
Met-Pro-p-nitroanilide
-
mutant enzyme H859A
0.988
Met-Pro-p-nitroanilide
-
mutant enzyme F822A
6.8
Pro-Gly
-
pH 7.8, 37°, erythrocyte lysate from healthy patients, activated by 0.1 mM MnCl2 and 20 mM Gly
8.6
Pro-Gly
-
pH 7.8, 37°, erythrocyte lysate from patients with prolidase (EC 3.4.13.9) deficiency, activated by 0.1 mM MnCl2 and 20 mM Gly
9.7
Pro-Gly
-
pH 7.8, 37°, erythrocyte lysate from patients with prolidase (EC 3.4.13.9) deficiency, activated by 0.1 mM MnCl2
13.2
Pro-Gly
-
in 50 mM Tris-HCl buffer (pH 7.8) with 2 mM or 20 mM MnCl2, at 37°C
13.2
Pro-Gly
-
in 50 mM Tris-HCl buffer (pH 7.8) with 2 mM or 20 mM MnCl2, at 37°C, in the presence of 10 mM L-methionine
13.8
Pro-Gly
-
pH 7.8, 37°, erythrocyte lysate from healthy patients, activated by 0.1 mM MnCl2
15.1
Pro-Gly
-
pH 7.8, 37°, erythrocyte lysate from healthy patients, activated by 20 mM Gly
15.85
Pro-Gly
-
in 50 mM Tris-HCl buffer (pH 7.8) with 2 mM or 20 mM MnCl2, at 37°C, in the presence of 50 mM L-methionine
16.7
Pro-Gly
-
pH 7.8, 37°, erythrocyte lysate from patients with prolidase (EC 3.4.13.9) deficiency, activated by 20 mM Gly
18.2
Pro-Gly
-
pH 7.8, 37°, erythrocyte lysate from patients with prolidase (EC 3.4.13.9) deficiency, without activating compound
23.57
Pro-Gly
-
in 50 mM Tris-HCl buffer (pH 7.8) with 2 mM or 20 mM MnCl2, at 37°C, in the presence of 10 mM D,L-homocysteine
26.9
Pro-Gly
-
pH 7.8, 37°, erythrocyte lysate from healthy patients, without activating compound
56.33
Pro-Gly
-
in 50 mM Tris-HCl buffer (pH 7.8) with 2 mM or 20 mM MnCl2, at 37°C, in the presence of 50 mM D,L-homocysteine
4.2
Pro-Met
-
pH 7.8, 37°C, erythrocyte lysate from patients with prolidase (EC 3.4.13.9) deficiency, activated by 20 mM Gly
5
Pro-Met
-
pH 7.8, 37°C, erythrocyte lysate from patients with prolidase (EC 3.4.13.9) deficiency, activated by 0.1 mM MnCl2 and 20 mM Gly
8.1
Pro-Met
-
pH 7.8, 37°C, erythrocyte lysate from patients with prolidase (EC 3.4.13.9) deficiency, without activating compound
8.35
Pro-Met
-
pH 7.8, 37°C, erythrocyte lysate from healthy patients, activated by 20 mM Gly
9.7
Pro-Met
-
pH 7.8, 37°C, erythrocyte lysate from patients with prolidase (EC 3.4.13.9) deficiency, activated by 0.1 mM MnCl2
9.9
Pro-Met
-
pH 7.8, 37°C, erythrocyte lysate from healthy patients, activated by 0.1 mM MnCl2 and 20 mM Gly
11.81
Pro-Met
-
in 50 mM Tris-HCl buffer (pH 7.8) with 2 mM or 20 mM MnCl2, at 37°C
13.24
Pro-Met
-
in 50 mM Tris-HCl buffer (pH 7.8) with 2 mM or 20 mM MnCl2, at 37°C, in the presence of 10 mM L-methionine
15.58
Pro-Met
-
in 50 mM Tris-HCl buffer (pH 7.8) with 2 mM or 20 mM MnCl2, at 37°C, in the presence of 50 mM L-methionine
17.6
Pro-Met
-
pH 7.8, 37°C, erythrocyte lysate from healthy patients, without activating compound
19.1
Pro-Met
-
pH 7.8, 37°C, erythrocyte lysate from healthy patients, activated by 0.1 mM MnCl2
22.3
Pro-Met
-
in 50 mM Tris-HCl buffer (pH 7.8) with 2 mM or 20 mM MnCl2, at 37°C, in the presence of 10 mM D,L-homocysteine
46.78
Pro-Met
-
in 50 mM Tris-HCl buffer (pH 7.8) with 2 mM or 20 mM MnCl2, at 37°C, in the presence of 50 mM D,L-homocysteine
3.2
Pro-Val
-
pH 7.8, 37°C, erythrocyte lysate from patients with prolidase (EC 3.4.13.9) deficiency, activated by 20 mM Gly
5.5
Pro-Val
-
pH 7.8, 37°C, erythrocyte lysate from patients with prolidase (EC 3.4.13.9) deficiency, activated by 0.1 mM MnCl2 and 20 mM Gly
7.9
Pro-Val
-
pH 7.8, 37°C, erythrocyte lysate from patients with prolidase (EC 3.4.13.9) deficiency, without activating compound
10.1
Pro-Val
-
pH 7.8, 37°C, erythrocyte lysate from patients with prolidase (EC 3.4.13.9) deficiency, activated by 0.1 mM MnCl2
14.7
Pro-Val
-
pH 7.8, 37°C, erythrocxyte lysate from healthy patients, activated by 20 mM Gly
17.3
Pro-Val
-
pH 7.8, 37°C, erythrocyte lysate from healthy patients, activated by 0.1 mM MnCl2 and 20 mM Gly
19.1
Pro-Val
-
in 50 mM Tris-HCl buffer (pH 7.8) with 2 mM or 20 mM MnCl2, at 37°C
19.54
Pro-Val
-
in 50 mM Tris-HCl buffer (pH 7.8) with 2 mM or 20 mM MnCl2, at 37°C, in the presence of 10 mM L-methionine
24.69
Pro-Val
-
in 50 mM Tris-HCl buffer (pH 7.8) with 2 mM or 20 mM MnCl2, at 37°C, in the presence of 50 mM L-methionine
26
Pro-Val
-
pH 7.8, 37°C, erythrocyte lysate from healthy patients, without activating compound
28.6
Pro-Val
-
pH 7.8, 37°C, erythrocxyte lysate from healthy patients, activated by 0.1 mM MnCl2
45.6
Pro-Val
-
in 50 mM Tris-HCl buffer (pH 7.8) with 2 mM or 20 mM MnCl2, at 37°C, in the presence of 10 mM D,L-homocysteine
75.2
Pro-Val
-
in 50 mM Tris-HCl buffer (pH 7.8) with 2 mM or 20 mM MnCl2, at 37°C, in the presence of 50 mM D,L-homocysteine
0.238
Ser-Pro-p-nitroanilide
-
wild type enzyme
2.249
Ser-Pro-p-nitroanilide
-
mutant enzyme F822A
7.462
Ser-Pro-p-nitroanilide
-
mutant enzyme H859A