3.2.2.23: DNA-formamidopyrimidine glycosylase
This is an abbreviated version!
For detailed information about DNA-formamidopyrimidine glycosylase, go to the full flat file.
Word Map on EC 3.2.2.23
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3.2.2.23
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strand
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comet
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glycosylases
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genotoxic
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purine
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single-stranded
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abasic
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duplex
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fpg-sensitive
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thymine
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excises
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7,8-dihydro-8-oxoguanine
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ape1
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ring-opened
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8-oxo-7,8-dihydroguanine
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8-oxodg
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endonucleases
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8-oxo-7,8-dihydro-2'-deoxyguanosine
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5-hydroxyuracil
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micronucleus
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transversions
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neils
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alkali-labile
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biomonitoring
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3-methyladenine-dna
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mispairs
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interstrand
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lesion-specific
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spiroiminodihydantoin
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bromate
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medicine
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2'-deoxyguanosine
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klenow
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ap-endonuclease
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uvrabc
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miscoding
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base-excision
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apurinic
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8-oxo-2'-deoxyguanosine
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monoadducts
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dihydrouracil
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enzyme-modified
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unhook
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mutyh
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endoiii
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analysis
- 3.2.2.23
- strand
- comet
- glycosylases
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genotoxic
- purine
-
single-stranded
-
abasic
- duplex
-
fpg-sensitive
- thymine
-
excises
- 7,8-dihydro-8-oxoguanine
- ape1
-
ring-opened
- 8-oxo-7,8-dihydroguanine
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8-oxodg
- endonucleases
-
8-oxo-7,8-dihydro-2'-deoxyguanosine
- 5-hydroxyuracil
-
micronucleus
-
transversions
-
neils
-
alkali-labile
-
biomonitoring
-
3-methyladenine-dna
- mispairs
-
interstrand
-
lesion-specific
- spiroiminodihydantoin
- bromate
- medicine
- 2'-deoxyguanosine
-
klenow
- ap-endonuclease
-
uvrabc
-
miscoding
-
base-excision
-
apurinic
- 8-oxo-2'-deoxyguanosine
-
monoadducts
- dihydrouracil
-
enzyme-modified
-
unhook
-
mutyh
-
endoiii
- analysis
Reaction
Synonyms
2,6-diamino-4-hydroxy-5(N-methyl)formamidopyrimidine-DNA glycosylase, 2,6-diamino-4-hydroxy-5N-formamidopyrimidine-DNA glycosylase, 2,6-diamino-4-hydroxy-5N-methyl-formamidopyrimidine-DNA glycosylase, 8-hydroxyguanine endonuclease, 8-oxoguanine DNA glycosylase, 8-oxoguanine-DNA glycosylase, deoxyribonucleate glycosidase, DNA glycohydrolase (releasing 2,6-diamino-4-hydroxy-5-(N-methyl)-formamidopyrimidine), DNA glycosylase, endonuclease VIII-like DNA glycosylase, Fapy DNA glycosylase, Fapy-DNA glycosylase, formamidopyrimidineDNA glycosylase, formamidopyrimidine DNA glycosylase, formamidopyrimidine glycosylase, formamidopyrimidine-DNA glycosyl hydrolase, formamidopyrimidine-DNA glycosylase, formamidopyrimidine-N-glycosylase, FPG, Fpg protein, FPG-1, FPG-2, Fpg-L, glycosidase, deoxyribonucleate formamidopyrimidine, More, Mtb-Fpg1, MutM, MutM1, NEH1, NEH2, Nei, NEIL1, NEIL3, OGG1
ECTree
3.2.2.23 DNA-formamidopyrimidine glycosylaseAdvanced search results
results (
results (KM Value
KM Value on EC 3.2.2.23 - DNA-formamidopyrimidine glycosylase
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KM VALUE [mM] 
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
IMAGE
0.0000039
13mer oligonucleotide duplex containing 8-oxoguanine
mutant R244E, pH 7.5, 37°C
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0.0000047
13mer oligonucleotide duplex containing 8-oxoguanine
mutant S208A, pH 7.5, 37°C
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0.000006
13mer oligonucleotide duplex containing 8-oxoguanine
mutant Q234R, pH 7.5, 37°C
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0.0000081
13mer oligonucleotide duplex containing 8-oxoguanine
wild-type, pH 7.5, 37°C
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0.0000088
13mer oligonucleotide duplex containing 8-oxoguanine
mutant Q234R/R244E, pH 7.5, 37°C
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0.0000063
23mer oligonucleotide duplex containing 8-oxoguanine
mutant R258Q, pH 7.5, 30°C
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0.0000081
23mer oligonucleotide duplex containing 8-oxoguanine
wild-type, pH 7.5, 30°C
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0.0000081
23mer oligonucleotide duplex containing 8-oxoguanine
mutant N168Q, pH 7.5, 30°C
-
0.000018
23mer oligonucleotide duplex containing 8-oxoguanine
mutant R108K, pH 7.5, 30°C
-
0.0000034
8-oxo-7,8-dihydroguanine:Cyt oligodeoxynucleotide
pH 7.4-7.5, mutant S326C
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0.0000034
8-oxo-7,8-dihydroguanine:Cyt oligodeoxynucleotide
pH 7.4-7.5, wild-type enzyme
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0.0000057
8-oxo-7,8-dihydroguanine:Cyt oligodeoxynucleotide
pH 7.4-7.5, mutant S231E
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0.0000061
8-oxo-7,8-dihydroguanine:Cyt oligodeoxynucleotide
pH 7.4-7.5, mutant D322N
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0.0000074
8-oxo-7,8-dihydroguanine:Cyt oligodeoxynucleotide
pH 7.4-7.5, mutant S280E
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0.0000075
8-oxo-7,8-dihydroguanine:Cyt oligodeoxynucleotide
pH 7.4-7.5, mutant S326E
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0.0000086
8-oxo-7,8-dihydroguanine:Cyt oligodeoxynucleotide
pH 7.4-7.5, mutant A288V
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0.0000092
8-oxo-7,8-dihydroguanine:Cyt oligodeoxynucleotide
pH 7.4-7.5, mutant S232E
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0.00001
8-oxo-7,8-dihydroguanine:Cyt oligodeoxynucleotide
pH 7.4-7.5, mutant S231E/S232E
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0.0000009
DNA
pH 7.5, 20°C, 24-oligomer DNA containing apurinic/apyrimidinic sites, lyase activity, K155A mutant Fpg
0.000019
DNA
pH 7.5, 20°C, 24-oligomer DNA containing AP sites, lyase activity, wild-type Fpg
0.00178
DNA containing 2,6-diamino-4-hydroxy-5-formamidopyrimidine residues
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pH 7.4, 37°C, K57R mutant Fpg, from DNA gamma-irradiated under NO2
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0.00187
DNA containing 2,6-diamino-4-hydroxy-5-formamidopyrimidine residues
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pH 7.4, 37°C, K57G mutant Fpg, from DNA gamma-irradiated under NO2
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0.00343 - 0.00376
DNA containing 2,6-diamino-4-hydroxy-5-formamidopyrimidine residues
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pH 7.4, 37°C, wild-type Fpg
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0.00422
DNA containing 2,6-diamino-4-hydroxy-5-formamidopyrimidine residues
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pH 7.4, 37°C, K57G mutant Fpg, from DNA treated with H2O2/Fe(III)-EDTA/asc
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0.00487
DNA containing 2,6-diamino-4-hydroxy-5-formamidopyrimidine residues
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pH 7.4, 37°C, K57R mutant Fpg, from DNA treated with H2O2/Fe(III)-EDTA/asc
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0.00078 - 0.00084
DNA containing 4,6-diamino-5-formamidopyrimidine residues
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pH 7.4, 37°C, K57G mutant Fpg
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0.00079 - 0.0009
DNA containing 4,6-diamino-5-formamidopyrimidine residues
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pH 7.4, 37°C, K57R mutant Fpg
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0.00107 - 0.00129
DNA containing 4,6-diamino-5-formamidopyrimidine residues
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pH 7.4, 37°C, wild-type Fpg
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0.0000069
DNA containing 7-hydro-8-oxoguanine residues
pH 7.5, 37°C, 24-oligomer, wild-type Fpg
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0.0000075
DNA containing 7-hydro-8-oxoguanine residues
pH 7.5, 37°C, 24-oligomer, K155A mutant Fpg
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0.000004
DNA containing 8-hydroxyguanine residues
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pH 7.8, 37°C, 34-oligomer, wild-type and K57R mutant Fpg
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0.000008
DNA containing 8-hydroxyguanine residues
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pH 7.8, 37°C, 34-oligomer, K57G mutant Fpg
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0.000008
DNA containing 8-hydroxyguanine residues
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pH 7.5, 20°C, dublex DNA containing a single 8-oxoguanine residue positioned opposite dC
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0.00093 - 0.00155
DNA containing 8-hydroxyguanine residues
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pH 7.4, 37°C, K57R mutant Fpg
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0.00109
DNA containing 8-hydroxyguanine residues
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pH 7.4, 37°C, wild-type Fpg, from DNA treated with H2O2/Fe(III)-EDTA/asc
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0.00126
DNA containing 8-hydroxyguanine residues
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pH 7.4, 37°C, K57G mutant Fpg
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0.00311
DNA containing 8-hydroxyguanine residues
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pH 7.4, 37°C, wild-type Fpg, from DNA gamma-irradiated under NO2
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0.022
DNA containing 8-oxo-guanine residues mispaired to adenine
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wild-type
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0.023
DNA containing 8-oxo-guanine residues mispaired to adenine
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mutant R108A
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0.0001
DNA containing 8-oxo-guanine residues mispaired to guanine
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wild-type
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0.0051
DNA containing 8-oxo-guanine residues mispaired to guanine
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mutant R108A
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0.00019
DNA containing 8-oxo-guanine residues mispaired to thymine
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wild-type
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0.0042
DNA containing 8-oxo-guanine residues mispaired to thymine
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mutant R108A
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0.000009 - 0.000011
DNA containing ring-opened N7-methylguanine residues
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pH 7.8, 37°C, wild-type, K57G and K57R mutant Fpg
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0.000023
DNA containing ring-opened N7-methylguanine residues
pH 7.5, 37°C, 23-oligomer, wild-type Fpg
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0.000029
DNA containing ring-opened N7-methylguanine residues
pH 7.5, 37°C, 23-oligomer, K155A mutant Fpg
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0.000038
DNA containing ring-opened N7-methylguanine residues
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pH 7.5, 37°C
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0.000041
DNA containing ring-opened N7-methylguanine residues
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pH 7.5, 20°C, dublex DNA containing a single Me-Fapy residue positioned opposite dC
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additional information
additional information
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Km values for excision of purine lesions from DNA treated with various free radical-generating systems
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additional information
additional information
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thermodynamic characterization of Fpg binding to lesion-containing 13mer DNA dublexes, binding affinity
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additional information
additional information
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Km for DNA substrate is affected by the base opposite the lesion
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additional information
additional information
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kinetic parameters of duplex oligonucleotides and mismatched duplexes
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additional information
additional information
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steady-state kinetics, overview
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additional information
additional information
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Fpg single-turnover kinetics, overview
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additional information
additional information
Fpg single-turnover kinetics, overview
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additional information
additional information
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Fpg single-turnover kinetics, overview
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additional information
additional information
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kinetics, detailed overview
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