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13mer oligonucleotide duplex containing 8-oxoguanine + H2O
8-oxoguanine + oligonucleotide
2,6-diamino-4-hydroxy-5-formamidopyrimidine-DNA + H2O
2,6-diamino-4-hydroxy-5-formamidopyrimidine + DNA
2,6-diamino-4-hydroxy-5-formamidopyrimidine:Cyt oligodeoxynucleotide + H2O
2,6-diamino-4-hydroxy-5-formamidopyrimidine + Cyt oligodeoxynucleotide
2,6-diamino-4-hydroxy-5-formamidopyrimidine:Cyt-DNA + H2O
2,6-diamino-4-hydroxy-5-formamidopyrimidine + Cyt-DNA
-
-
-
?
23mer oligonucleotide duplex containing 8-oxoguanine + H2O
8-oxoguanine + oligonucleotide
-
-
-
?
5,6-dihydrouracil-DNA + H2O
5,6-dihydrouracil + DNA
53mer containing 8-oxoguanine + H2O
53mer with an abasic site + 8-oxoguanine
-
-
-
?
60mer containing 8-oxoguanine + H2O
60mer with an abasic site + 8-oxoguanine
8-oxo-7,8-dihydroguanine:Cyt oligodeoxynucleotide + H2O
8-oxo-7,8-dihydroguanine + Cyt oligodeoxynucleotide
8-oxo-7,8-dihydroguanine:Cyt-DNA + H2O
8-oxo-7,8-dihydroguanine + Cyt-DNA
-
-
-
?
depurinated supercoiled plasmid DNA + H2O
?
-
the degree of supercoiling of assay plasmid DNA does not affect enzyme activity
-
-
?
DNA containing 2'-deoxy-8-oxonebularine residues + H2O
?
23mer oligonucleotide containing a single site, very poor substrate
-
-
?
DNA containing 2,6-diamino-4-hydroxy-5-formamidopyrimidine residues + H2O
2,6-diamino-4-hydroxy-5-formamidopyrimidine + DNA
DNA containing 2,6-diamino-4-hydroxy-5-formamidopyrimidine residues + H2O
DNA + 2,6-diamino-4-hydroxy-5-formamidopyrimidine
DNA containing 2,6-diamino-4-hydroxyformamidopyrimidine residues + H2O
2,6-diamino-4-hydroxyformamidopyrimidine + DNA
-
repair of the major DNA lesions 7,8-dihydro-8-oxoguanine and 2,6-diamino-4-hydroxyformamidopyrimidine formed by reactive oxidative species
-
-
?
DNA containing 4,6-diamino-5-formamidopyrimidine residues + H2O
4,6-diamino-5-formamidopyrimidine + DNA
DNA containing 4,6-diamino-5-formamidopyrimidine residues + H2O
DNA + 4,6-diamino-5-formamidopyrimidine
DNA containing 5,6-dihydrothymine residues + H2O
?
-
dublex 33mer oligonucleotide, poor substrate
-
-
?
DNA containing 5,6-dihydrouracil + H2O
DNA + 5,6-dihydrouracil
-
-
-
-
?
DNA containing 5-hydroxy-2'-deoxyuridine + H2O
?
DNA containing 5-hydroxy-5-methylhydantoin residues opposite cytosine + H2O
5-hydroxy-5-methylhydantoin + DNA
-
excellent substrate when the lesion is opposite a cytosine, poor substrate when the lesion is opposite a adenine
-
-
?
DNA containing 5-hydroxycytosine residues + H2O
5-hydroxycytosine + DNA
DNA containing 5-hydroxycytosine residues + H2O
?
DNA containing 5-hydroxyuracil residues + H2O
5-hydroxyuracil + DNA
DNA containing 7,8-dihydro-8-oxoguanine residues + H2O
DNA + 7,8-dihydro-8-oxoguanine
-
-
-
-
?
DNA containing 7-deaza-2'-deoxyguanosine + H2O
7-deaza-2'-deoxyguanosine + DNA
DNA containing 7-hydro-8-oxoguanine + H2O
7-hydro-8-oxoguanine + DNA
DNA containing 7-hydro-8-oxoguanine residues + H2O
DNA + 7-hydro-8-oxoguanine
DNA containing 7-methyl-7-deazaguanine residues + H2O
7-methyl-7-deazaguanine + DNA
-
-
-
-
?
DNA containing 7-methyl-8-oxo-2'-deoxyguanosine + H2O
7-methyl-8-oxo-2'-deoxyguanosine + DNA
DNA containing 8-hydroxyadenine + H2O
DNA + 8-hydroxyguanine
-
-
-
-
?
DNA containing 8-hydroxyadenine residues + H2O
8-hydroxyadenine + DNA
-
poor substrate
-
-
?
DNA containing 8-hydroxyguanine residues + H2O
8-hydroxyguanine + DNA
DNA containing 8-hyroxyguanine + H2O
?
-
-
-
-
?
DNA containing 8-oxo-2'-deoxyguanosine + H2O
8-oxo-2'-deoxyguanosine + DNA
DNA containing 8-oxo-2'-deoxyguanosine residues + H2O
DNA + 8-oxo-2'-deoxyguanosine
-
-
-
-
?
DNA containing 8-oxo-2'-deoxyinosine + H2O
?
-
dublex
-
-
?
DNA containing 8-oxo-7,8-dihydroguanine residues + H2O
DNA + 8-hydroxyguanine
-
-
-
-
?
DNA containing 8-oxo-7,8-dihydroguanine residues + H2O
DNA + 8-oxo-7,8-dihydroguanine
-
-
-
?
DNA containing 8-oxo-7,8-dihydropurine + H2O
8-oxo-7,8-dihydropurine + DNA
-
poor substrate when the lesion is opposite a cytosine
-
-
?
DNA containing 8-oxo-guanine residues + H2O
DNA + 8-oxoguanine
-
-
-
-
?
DNA containing 8-oxo-guanine residues mispaired to adenine + H2O
DNA + 8-oxoguanine
-
-
-
-
?
DNA containing 8-oxo-guanine residues mispaired to guanine + H2O
DNA + 8-oxoguanine
-
-
-
-
?
DNA containing 8-oxo-guanine residues mispaired to thymine + H2O
DNA + 8-oxoguanine
-
-
-
-
?
DNA containing 8-oxoguanine opposite A + H2O
8-oxoguanine + DNA
poor substrate, analysis of rate constants of conformational transitions
-
-
?
DNA containing 8-oxoguanine opposite C + H2O
8-oxoguanine + DNA
good substrate, analysis of rate constants of conformational transitions
-
-
?
DNA containing 8-oxoguanine opposite G + H2O
8-oxoguanine + DNA
analysis of rate constants of conformational transitions
-
-
?
DNA containing 8-oxoguanine opposite T + H2O
8-oxoguanine + DNA
analysis of rate constants of conformational transitions
-
-
?
DNA containing 8-oxoguanine residues + H2O
8-oxoguanine + DNA
DNA containing 8-oxoguanine residues + H2O
DNA + 8-oxoguanine
DNA containing 8-thio-2'-deoxyguanosine + H2O
8-thio-2'-deoxyguanosine + DNA
DNA containing dihydrothymine residues + H2O
dihydrothymine + DNA
-
-
-
-
?
DNA containing dihydrouracil + H2O
8-oxoguanine + DNA
-
-
-
?
DNA containing dihydrouracil residues + H2O
?
active as a DNA glycosylase/AP lyase with dihydrouracil 31mer dublex oligo substrate, similar activity with A, C or G opposite the lesion, reduced with T opposite
-
-
?
DNA containing formamidopyrimidine-guanine residues + H2O
4,6-diamino-5-formamidopyrimidine + DNA
DNA containing guanidinohydantoin + H2O
guanidinohydantoin + DNA
-
for DNA duplex length of 30 bp, the excision efficiency in pairs with C, G, or T is similar to 8-oxoguanine. Opposite A, the base removal activity is more efficient than removal of 8-oxoguanine
-
-
?
DNA containing methylated formamidopyrimidine-guanine residues + H2O
2,6-diamino-4-hydroxy-5-(N-methyl)formamidopyrimidine + DNA
DNA containing oxoG opposite C or A + H2O
2,6-diamino-4-hydroxy-5-formamidopyrimidine + DNA
-
-
-
?
DNA containing ring-opened N7-methylguanine + H2O
2,6-diamino-4-hydroxy-5-(N-methyl)formamidopyrimidine + DNA
-
repairs oxidative DNA damage by efficiently removing formamidopyrimidine lesions and 8-oxoguanine residues from DNA
-
-
?
DNA containing ring-opened N7-methylguanine residues + H2O
2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine + DNA
DNA containing spiroiminodihydantoin + H2O
spiroiminodihydantoin + DNA
-
for DNA duplex length of 30 bp, the excision efficiency in pairs with C, G, or T is similar to 8-oxoguanine. Opposite A, the base removal activity is more efficient than removal of 8-oxoguanine
-
-
?
DNA containing tetrahydrofuran residues + H2O
tetrahydrofuran + DNA
ds oligodeoxynucleotides containing N5-alkyl formamidopyrimidine + H2O
2,6-diamino-4-hydroxy-5-formamidopyrimidine + ds oligonucleotide
-
-
-
?
oligonucleotide containing 8-oxo-2'-deoxyguanosine residue + H2O
oligonucleotide + 8-oxo-2'-deoxyguanosine
-
-
-
-
?
oligonucleotide containing abasic site residue + H2O
oligonucleotide + abasic site
-
-
-
-
?
oligonucleotide containing tetrahydrofuran residue + H2O
oligonucleotide + tetrahydrofuran
-
-
-
-
?
ss oligodeoxynucleotides containing N5-alkyl formamidopyrimidine + H2O
2,6-diamino-4-hydroxy-5-formamidopyrimidine + ds oligonucleotide
DNA base alkylation damage induced by nitrogen mustard
-
-
?
additional information
?
-
13mer oligonucleotide duplex containing 8-oxoguanine + H2O
8-oxoguanine + oligonucleotide
-
-
-
-
?
13mer oligonucleotide duplex containing 8-oxoguanine + H2O
8-oxoguanine + oligonucleotide
-
-
-
?
2,6-diamino-4-hydroxy-5-formamidopyrimidine-DNA + H2O
2,6-diamino-4-hydroxy-5-formamidopyrimidine + DNA
-
-
-
-
?
2,6-diamino-4-hydroxy-5-formamidopyrimidine-DNA + H2O
2,6-diamino-4-hydroxy-5-formamidopyrimidine + DNA
-
-
-
-
?
2,6-diamino-4-hydroxy-5-formamidopyrimidine:Cyt oligodeoxynucleotide + H2O
2,6-diamino-4-hydroxy-5-formamidopyrimidine + Cyt oligodeoxynucleotide
-
-
-
-
?
2,6-diamino-4-hydroxy-5-formamidopyrimidine:Cyt oligodeoxynucleotide + H2O
2,6-diamino-4-hydroxy-5-formamidopyrimidine + Cyt oligodeoxynucleotide
-
-
-
?
5,6-dihydrouracil-DNA + H2O
5,6-dihydrouracil + DNA
-
DHU is excised from DNA by a number of DNA glycosylases including Fpg and Nei
-
-
?
5,6-dihydrouracil-DNA + H2O
5,6-dihydrouracil + DNA
-
DHU is formed in DNA from cytosine under the action of OH radicals under ionizing radiation
-
-
?
60mer containing 8-oxoguanine + H2O
60mer with an abasic site + 8-oxoguanine
-
-
-
?
60mer containing 8-oxoguanine + H2O
60mer with an abasic site + 8-oxoguanine
-
-
-
?
8-oxo-7,8-dihydroguanine:Cyt oligodeoxynucleotide + H2O
8-oxo-7,8-dihydroguanine + Cyt oligodeoxynucleotide
-
-
-
-
?
8-oxo-7,8-dihydroguanine:Cyt oligodeoxynucleotide + H2O
8-oxo-7,8-dihydroguanine + Cyt oligodeoxynucleotide
-
-
-
?
DNA + H2O
?
both AtFPG-1 and -2 cleave DNA containing apurinic sites and UV-irradiated and oxidized DNA
-
-
?
DNA + H2O
?
catalyzes the initial steps in the repair of DNA containing oxidized purines
-
-
?
DNA + H2O
?
-
substrate specificity
-
-
?
DNA + H2O
?
-
enzyme has N-glycosylase and apurinic/apyrimidinic lyase activity
-
-
?
DNA + H2O
?
-
DNA base excision repair enzyme
-
-
?
DNA + H2O
?
-
substrate specificity
-
-
?
DNA + H2O
?
-
with apurinic/apyrimidinic lyase activity, catalyzes beta and delta elimination reactions
-
-
?
DNA + H2O
?
with apurinic/apyrimidinic lyase activity, catalyzes beta and delta elimination reactions
-
-
?
DNA + H2O
?
-
role of Lys-155 for substrate binding and product release, AP lyase mechanism
-
-
?
DNA + H2O
?
-
FpG-DNA interactions establish contacts with DNA ligands, which span no more than 9 base-pairs, structural studies of Fpg-DNA complexes
-
-
?
DNA + H2O
?
-
excises oxidized purines from damaged DNA, Schiff base intermediate, enzyme structure, bilobal protein with a wide, positive charged DNA-binding groove and a helix-2-turn-helix motif that participates in DNA binding, damage recognition, catalytic mechanism
-
-
?
DNA + H2O
?
-
removes oxidized purines from oxidatively damaged DNA
-
-
?
DNA + H2O
?
removes oxidized purines from oxidatively damaged DNA
-
-
?
DNA + H2O
?
-
catalytic mechanism involves formation of Schiff base intermediate between DNA containing an oxidized residue and the N-terminal Pro-2 of Fpg, mendatory role of P-2 in 7,8-dihydro-8-oxoguanine-DNA glycosylase and AP lyase activity, but less in 2,6-diamino-4-hydroxy-5-N-methyl-formamidopyrimidine-DNA glycosylase activity
-
-
?
DNA + H2O
?
-
active site is located within the first 73 amino acids of the N-terminus
-
-
?
DNA + H2O
?
-
excises purine bases with ring-opened imidazoles, associated activity that nicks DNA at apurinic/apyrimidinic sites, mechanism of cleavage involves beta elimination
-
-
?
DNA + H2O
?
-
enzyme has N-glycosylase and apurinic/apyrimidinic lyase activity
646932, 646933, 646935, 646936, 646943, 646944, 646946, 646948, 646950, 646951, 646952, 646954 -
-
?
DNA + H2O
?
enzyme has N-glycosylase and apurinic/apyrimidinic lyase activity
-
-
?
DNA + H2O
?
-
enzyme catalyzes the nicking of both the phosphodiester bonds 3' and 5' of apurinic or apyrimidinic sites in DNA so that the base-free deoxyribose is replaced by a gap limited by 3'-phosphate and 5'-phosphate ends, the 2 nickings are not the result of hydrolytic processes, the enzyme rather catalyzes a beta-elimination reaction immediately followed by a delta-elimination
-
-
?
DNA + H2O
?
-
DNA repair enzyme specific for the removal of purine-derived lesions from DNA damaged by free radicals and other oxidative processes
-
-
?
DNA + H2O
?
-
plays an important role in base excision repair of oxidatively damaged DNA
-
-
?
DNA + H2O
?
-
involved in the repair of oxidized purines generated in the genome by endogenous or exogenous oxidative stress
-
-
?
DNA + H2O
?
-
biological substrates are purine oxidation products
-
-
?
DNA + H2O
?
-
DNA base excision repair enzyme
-
-
?
DNA + H2O
?
DNA base excision repair enzyme
-
-
?
DNA + H2O
?
-
bifunctional base excision repair enzyme: DNA glycosylase/AP lyase
-
-
?
DNA + H2O
?
-
with apurinic/apyrimidinic lyase activity, catalyzes beta and delta elimination reactions
-
-
?
DNA + H2O
?
-
FpG-DNA interactions establish contacts with DNA ligands, which span no more than 9 base-pairs, structural studies of Fpg-DNA complexes
-
-
?
DNA + H2O
?
-
enzyme has N-glycosylase and apurinic/apyrimidinic lyase activity
-
-
?
DNA + H2O
?
-
bifunctional base excision repair enzyme: DNA glycosylase/AP lyase
-
-
?
DNA + H2O
?
-
removes oxidized purines from oxidatively damaged DNA
-
-
?
DNA + H2O
?
-
enzyme has N-glycosylase and apurinic/apyrimidinic lyase activity
-
-
?
DNA + H2O
?
-
involved in the repair of oxidized purines generated in the genome by endogenous or exogenous oxidative stress
-
-
?
DNA + H2O
?
-
removes oxidized purines from oxidatively damaged DNA
-
-
?
DNA + H2O
?
-
enzyme has N-glycosylase and apurinic/apyrimidinic lyase activity
-
-
?
DNA + H2O
?
-
enzyme has N-glycosylase and apurinic/apyrimidinic lyase activity
-
-
?
DNA + H2O
?
-
DNA base excision repair enzyme
-
-
?
DNA + H2O
?
substrate specificity
-
-
?
DNA + H2O
?
with apurinic/apyrimidinic lyase activity, catalyzes beta and delta elimination reactions
-
-
?
DNA + H2O
?
excises formamidopyrimidines from damaged DNA and oxidized pyrimidines and 8-oxoguanine from oligodeoxynucleotides, role of the N-terminal Pro as its active site
-
-
?
DNA + H2O
?
enzyme has N-glycosylase and apurinic/apyrimidinic lyase activity
-
-
?
DNA + H2O
?
involved in replication-associated repair of oxidized bases
-
-
?
DNA + H2O
?
DNA base excision repair enzyme
-
-
?
DNA + H2O
?
-
removes a wide range of oxidized purines, such as 8-oxoguanine and imidazole ring-opened purines, from oxidatively damaged DNA, DNA glycosylase/AP lyase activity, substrate recognition
-
-
?
DNA + H2O
?
-
removes oxidized purines from oxidatively damaged DNA
-
-
?
DNA + H2O
?
-
enzyme has N-glycosylase and apurinic/apyrimidinic lyase activity
-
-
?
DNA + H2O
?
enzyme has N-glycosylase and apurinic/apyrimidinic lyase activity
-
-
?
DNA + H2O
?
important role in protecting DNA against oxidative free radicals and reactive oxygen-derived species
-
-
?
DNA + H2O
?
-
bifunctional base excision repair enzyme: DNA glycosylase/AP lyase
-
-
?
DNA containing 2,6-diamino-4-hydroxy-5-formamidopyrimidine residues + H2O
2,6-diamino-4-hydroxy-5-formamidopyrimidine + DNA
-
similar specificity as FapyAde-DNA, better substrate than 8-hydroxyguanine-DNA
-
-
?
DNA containing 2,6-diamino-4-hydroxy-5-formamidopyrimidine residues + H2O
2,6-diamino-4-hydroxy-5-formamidopyrimidine + DNA
-
FapyGua
-
-
?
DNA containing 2,6-diamino-4-hydroxy-5-formamidopyrimidine residues + H2O
2,6-diamino-4-hydroxy-5-formamidopyrimidine + DNA
-
-
-
-
?
DNA containing 2,6-diamino-4-hydroxy-5-formamidopyrimidine residues + H2O
2,6-diamino-4-hydroxy-5-formamidopyrimidine + DNA
-
removal with similar specificity as 4,6-diamino-5-formamidopyrimidine and 8-hydroxyguanine
-
?
DNA containing 2,6-diamino-4-hydroxy-5-formamidopyrimidine residues + H2O
2,6-diamino-4-hydroxy-5-formamidopyrimidine + DNA
-
FapyGua
-
?
DNA containing 2,6-diamino-4-hydroxy-5-formamidopyrimidine residues + H2O
2,6-diamino-4-hydroxy-5-formamidopyrimidine + DNA
-
FapyGua
-
-
?
DNA containing 2,6-diamino-4-hydroxy-5-formamidopyrimidine residues + H2O
2,6-diamino-4-hydroxy-5-formamidopyrimidine + DNA
FapyGua
-
-
?
DNA containing 2,6-diamino-4-hydroxy-5-formamidopyrimidine residues + H2O
2,6-diamino-4-hydroxy-5-formamidopyrimidine + DNA
FapyGua
-
-
?
DNA containing 2,6-diamino-4-hydroxy-5-formamidopyrimidine residues + H2O
2,6-diamino-4-hydroxy-5-formamidopyrimidine + DNA
-
-
-
-
?
DNA containing 2,6-diamino-4-hydroxy-5-formamidopyrimidine residues + H2O
DNA + 2,6-diamino-4-hydroxy-5-formamidopyrimidine
-
-
-
-
?
DNA containing 2,6-diamino-4-hydroxy-5-formamidopyrimidine residues + H2O
DNA + 2,6-diamino-4-hydroxy-5-formamidopyrimidine
-
-
-
?
DNA containing 2,6-diamino-4-hydroxy-5-formamidopyrimidine residues + H2O
DNA + 2,6-diamino-4-hydroxy-5-formamidopyrimidine
-
-
-
-
?
DNA containing 4,6-diamino-5-formamidopyrimidine residues + H2O
4,6-diamino-5-formamidopyrimidine + DNA
-
similar specificity as FapyGua-DNA, better substrate than 8-hydroxyguanine-DNA
-
-
?
DNA containing 4,6-diamino-5-formamidopyrimidine residues + H2O
4,6-diamino-5-formamidopyrimidine + DNA
-
FapyAde
-
-
?
DNA containing 4,6-diamino-5-formamidopyrimidine residues + H2O
4,6-diamino-5-formamidopyrimidine + DNA
-
-
-
-
?
DNA containing 4,6-diamino-5-formamidopyrimidine residues + H2O
4,6-diamino-5-formamidopyrimidine + DNA
-
removal with similar specificity as 2,6-diamino-4-hydroxy-5-formamidopyrimidine and 8-hydroxyguanine
-
?
DNA containing 4,6-diamino-5-formamidopyrimidine residues + H2O
4,6-diamino-5-formamidopyrimidine + DNA
-
FapyAde
-
?
DNA containing 4,6-diamino-5-formamidopyrimidine residues + H2O
4,6-diamino-5-formamidopyrimidine + DNA
-
FapyAde
-
-
?
DNA containing 4,6-diamino-5-formamidopyrimidine residues + H2O
4,6-diamino-5-formamidopyrimidine + DNA
FapyAde
-
-
?
DNA containing 4,6-diamino-5-formamidopyrimidine residues + H2O
4,6-diamino-5-formamidopyrimidine + DNA
FapyAde
-
-
?
DNA containing 4,6-diamino-5-formamidopyrimidine residues + H2O
4,6-diamino-5-formamidopyrimidine + DNA
-
-
-
-
?
DNA containing 4,6-diamino-5-formamidopyrimidine residues + H2O
DNA + 4,6-diamino-5-formamidopyrimidine
-
-
-
-
?
DNA containing 4,6-diamino-5-formamidopyrimidine residues + H2O
DNA + 4,6-diamino-5-formamidopyrimidine
-
-
-
?
DNA containing 4,6-diamino-5-formamidopyrimidine residues + H2O
DNA + 4,6-diamino-5-formamidopyrimidine
-
-
-
-
?
DNA containing 5-hydroxy-2'-deoxyuridine + H2O
?
-
double-stranded oligonucleotides, N-glycosylase/beta,delta-elimination reaction
-
-
?
DNA containing 5-hydroxy-2'-deoxyuridine + H2O
?
dublex oligodeoxynucleotide containing 5-hydroxyuracil when paired with G
-
-
?
DNA containing 5-hydroxycytosine residues + H2O
5-hydroxycytosine + DNA
-
-
-
-
?
DNA containing 5-hydroxycytosine residues + H2O
5-hydroxycytosine + DNA
-
-
-
-
?
DNA containing 5-hydroxycytosine residues + H2O
?
-
double-stranded oligonucleotides containing 5-hydroxy-2'-deoxycytidine, N-glycosylase/beta,delta-elimination reaction
-
-
?
DNA containing 5-hydroxycytosine residues + H2O
?
-
dublex 33mer oligonucleotide, excision mechanism
-
-
?
DNA containing 5-hydroxyuracil residues + H2O
5-hydroxyuracil + DNA
-
-
-
-
?
DNA containing 5-hydroxyuracil residues + H2O
5-hydroxyuracil + DNA
-
-
-
-
?
DNA containing 7-deaza-2'-deoxyguanosine + H2O
7-deaza-2'-deoxyguanosine + DNA
-
-
-
-
?
DNA containing 7-deaza-2'-deoxyguanosine + H2O
7-deaza-2'-deoxyguanosine + DNA
-
-
-
-
?
DNA containing 7-hydro-8-oxoguanine + H2O
7-hydro-8-oxoguanine + DNA
-
repairs oxidative DNA damage by efficiently removing formamidopyrimidine lesions and 8-oxoguanine residues from DNA
-
-
?
DNA containing 7-hydro-8-oxoguanine + H2O
7-hydro-8-oxoguanine + DNA
-
dublex, primary physiological substrate, DNA repair
-
-
?
DNA containing 7-hydro-8-oxoguanine residues + H2O
DNA + 7-hydro-8-oxoguanine
-
-
-
-
?
DNA containing 7-hydro-8-oxoguanine residues + H2O
DNA + 7-hydro-8-oxoguanine
24-oligomer, Lys-155 directly interacts with the C8 oxygen of 8-oxopurines involving proton transfer or transient formation of an ion pair between enzyme and substrate, mechanism
-
?
DNA containing 7-hydro-8-oxoguanine residues + H2O
DNA + 7-hydro-8-oxoguanine
DNA glycosylase/AP lyase activity
-
?
DNA containing 7-hydro-8-oxoguanine residues + H2O
DNA + 7-hydro-8-oxoguanine
-
the C-8 keto group of 8-oxodeoxyguanine and the carbonyl moiety of formamidopyrimidine enable the enzyme to recognize and bind duplex DNA containing modified bases
-
-
?
DNA containing 7-hydro-8-oxoguanine residues + H2O
DNA + 7-hydro-8-oxoguanine
-
mechanism involving protonation at O-6 of 8-oxodeoxyguanine
-
-
?
DNA containing 7-hydro-8-oxoguanine residues + H2O
DNA + 7-hydro-8-oxoguanine
-
dublex 20-oligomer, catalytic mechanism with enzyme-substrate Schiff base intermediate, amino terminal localization of the catalytic site, C-8 keto group of 8-oxodeoxyguanine plays a critical role in binding enzyme
-
-
?
DNA containing 7-hydro-8-oxoguanine residues + H2O
DNA + 7-hydro-8-oxoguanine
-
kinetic mechanism, 3 activities: DNA-glycosylase, beta-elimination/AP-lyase and delta elimination, 12-nucleotide dublex containing 8-oxo-G in the sixth position of one strand, conformational transitions of Fpg protein during the catalytic process
-
-
?
DNA containing 7-hydro-8-oxoguanine residues + H2O
DNA + 7-hydro-8-oxoguanine
-
mechanism involving protonation at O-6 of 8-oxodeoxyguanine
-
-
?
DNA containing 7-hydro-8-oxoguanine residues + H2O
DNA + 7-hydro-8-oxoguanine
-
dublex 20-oligomer, catalytic mechanism with enzyme-substrate Schiff base intermediate, amino terminal localization of the catalytic site, C-8 keto group of 8-oxodeoxyguanine plays a critical role in binding enzyme
-
-
?
DNA containing 7-hydro-8-oxoguanine residues + H2O
DNA + 7-hydro-8-oxoguanine
excises 8-oxoguanine from 31mer oligodeoxynucleotide, most active with G, followed by T, opposite the lesion, weak activity with C or A opposite
-
-
?
DNA containing 7-hydro-8-oxoguanine residues + H2O
DNA + 7-hydro-8-oxoguanine
-
removes 8-oxoguanine from oxidatively damaged DNA
-
-
?
DNA containing 7-hydro-8-oxoguanine residues + H2O
DNA + 7-hydro-8-oxoguanine
-
DNA glycosylase/AP lyase activity
-
-
?
DNA containing 7-methyl-8-oxo-2'-deoxyguanosine + H2O
7-methyl-8-oxo-2'-deoxyguanosine + DNA
-
-
-
-
?
DNA containing 7-methyl-8-oxo-2'-deoxyguanosine + H2O
7-methyl-8-oxo-2'-deoxyguanosine + DNA
-
-
-
-
?
DNA containing 8-hydroxyguanine residues + H2O
8-hydroxyguanine + DNA
AtFPG-1, but not AtFPG-2, cleaves double-stranded oligonucleotides containing 8-oxoguanine
-
-
?
DNA containing 8-hydroxyguanine residues + H2O
8-hydroxyguanine + DNA
-
formamidopyrimidines are preferred over 8-hydroxyguanine
-
-
?
DNA containing 8-hydroxyguanine residues + H2O
8-hydroxyguanine + DNA
-
-
-
-
?
DNA containing 8-hydroxyguanine residues + H2O
8-hydroxyguanine + DNA
-
DNA glycosylase/AP lyase activity
-
-
?
DNA containing 8-hydroxyguanine residues + H2O
8-hydroxyguanine + DNA
-
34mer oligonucleotide containing a single 7,8-dihydro-8-oxoguanine residue
-
-
?
DNA containing 8-hydroxyguanine residues + H2O
8-hydroxyguanine + DNA
-
dublex oligodeoxynucleotides containing 8-oxo-7,8-dihydro-2-deoxyguanosine positioned opposite dC, dG or dT are cleaved, but not opposite dA or single-stranded DNA, cleaves 3 and 5 to the modified base
-
-
?
DNA containing 8-hydroxyguanine residues + H2O
8-hydroxyguanine + DNA
-
comparison of repair activities of human OGG1 and Escherichia coli Fpg, enzymes show distinct preferences for the base opposite 8-oxoguanine, mechanism via Schiff base intermediate
treatment of 7,8-dihydro-8-oxoguanine with Fpg results in delta-elimination products
?
DNA containing 8-hydroxyguanine residues + H2O
8-hydroxyguanine + DNA
-
mendatory role of P-2 in 7,8-dihydro-8-oxoguanine-DNA glycosylase activity
-
-
?
DNA containing 8-hydroxyguanine residues + H2O
8-hydroxyguanine + DNA
-
7,8-dihydro-8-oxo-2-deoxyguanosine, natural substrate, substrate recognition, mechanism
-
-
?
DNA containing 8-hydroxyguanine residues + H2O
8-hydroxyguanine + DNA
-
readily incises dublexes with cytosine, thymine or guanine opposite, but much slower with adenine opposite 7,8-dihydro-8-oxoguanine, 2 activities: DNA-glycosylase and DNA nicking at abasic sites
treatment of 7,8-dihydro-8-oxoguanine with Fpg results in delta-elimination products
?
DNA containing 8-hydroxyguanine residues + H2O
8-hydroxyguanine + DNA
-
7,8-dihydro-8-oxoguanine opposite C dublex DNA, formation of a Schiff base intermediate, important role for Lys-57 in the 7,8-dihydro-8-oxoG-DNA glycolase activity in vitro and in vivo
-
-
?
DNA containing 8-hydroxyguanine residues + H2O
8-hydroxyguanine + DNA
-
7,8-dihydro-8-oxoguanine-DNA
-
-
?
DNA containing 8-hydroxyguanine residues + H2O
8-hydroxyguanine + DNA
-
7,8-dihydro-8-oxoguanine-DNA
treatment of 7,8-dihydro-8-oxoguanine with Fpg results in delta-elimination products
?
DNA containing 8-hydroxyguanine residues + H2O
8-hydroxyguanine + DNA
-
removal with similar specificity as 4,6-diamino-5-formamidopyrimidine and 2,6-diamino-4-hydroxy-5-formamidopyrimidine, important role of Lys-57 in Fpg activity for 8-hydroxyguanine, but lesser significant role for formamidopyrimidines, Pro-2 is critical for substrate recognition and in catalysis of its excision
-
-
?
DNA containing 8-hydroxyguanine residues + H2O
8-hydroxyguanine + DNA
-
54 nt DNA oligomer, CoFpg and ZnFpg are equally active at cleaving the DNA at the site of the oxidized guanine
-
-
?
DNA containing 8-hydroxyguanine residues + H2O
8-hydroxyguanine + DNA
-
natural substrate: 7,8-dihydro-8-oxo-dG, DNA base excision repair
-
-
?
DNA containing 8-hydroxyguanine residues + H2O
8-hydroxyguanine + DNA
-
dublex, primary physiological substrate is 7,8-dihydro-8-oxoguanine-DNA, DNA repair
-
-
?
DNA containing 8-hydroxyguanine residues + H2O
8-hydroxyguanine + DNA
-
repair of the major DNA lesions 7,8-dihydro-8-oxoguanine and 2,6-diamino-4-hydroxyformamidopyrimidine formed by reactive oxidative species
-
-
?
DNA containing 8-hydroxyguanine residues + H2O
8-hydroxyguanine + DNA
-
-
-
-
?
DNA containing 8-hydroxyguanine residues + H2O
8-hydroxyguanine + DNA
-
DNA glycosylase/AP lyase activity
-
-
?
DNA containing 8-hydroxyguanine residues + H2O
8-hydroxyguanine + DNA
-
34mer oligonucleotide containing a single 7,8-dihydro-8-oxoguanine residue
-
-
?
DNA containing 8-hydroxyguanine residues + H2O
8-hydroxyguanine + DNA
34mer oligonucleotide containing a single 7,8-dihydro-8-oxoguanine residue
-
-
?
DNA containing 8-hydroxyguanine residues + H2O
8-hydroxyguanine + DNA
readily incises dublexes with cytosine, thymine or guanine opposite, but much slower with adenine opposite 7,8-dihydro-8-oxoguanine, 2 activities: DNA-glycosylase and DNA nicking at abasic sites
-
-
?
DNA containing 8-oxo-2'-deoxyguanosine + H2O
8-oxo-2'-deoxyguanosine + DNA
-
-
-
-
?
DNA containing 8-oxo-2'-deoxyguanosine + H2O
8-oxo-2'-deoxyguanosine + DNA
-
-
-
-
?
DNA containing 8-oxoguanine residues + H2O
8-oxoguanine + DNA
-
-
-
-
?
DNA containing 8-oxoguanine residues + H2O
8-oxoguanine + DNA
-
8-oxoguanine residues opposite cytosine
-
-
?
DNA containing 8-oxoguanine residues + H2O
8-oxoguanine + DNA
-
-
-
-
?
DNA containing 8-oxoguanine residues + H2O
DNA + 8-oxoguanine
-
-
-
-
?
DNA containing 8-oxoguanine residues + H2O
DNA + 8-oxoguanine
best substrate
-
-
?
DNA containing 8-oxoguanine residues + H2O
DNA + 8-oxoguanine
best substrate
-
-
?
DNA containing 8-oxoguanine residues + H2O
DNA + 8-oxoguanine
best substrate
-
-
?
DNA containing 8-oxoguanine residues + H2O
DNA + 8-oxoguanine
best substrate
-
-
?
DNA containing 8-oxoguanine residues + H2O
DNA + 8-oxoguanine
best substrate
-
-
?
DNA containing 8-thio-2'-deoxyguanosine + H2O
8-thio-2'-deoxyguanosine + DNA
-
-
-
-
?
DNA containing 8-thio-2'-deoxyguanosine + H2O
8-thio-2'-deoxyguanosine + DNA
-
-
-
-
?
DNA containing formamidopyrimidine-guanine residues + H2O
4,6-diamino-5-formamidopyrimidine + DNA
-
-
-
?
DNA containing formamidopyrimidine-guanine residues + H2O
4,6-diamino-5-formamidopyrimidine + DNA
-
-
-
?
DNA containing formamidopyrimidine-guanine residues + H2O
4,6-diamino-5-formamidopyrimidine + DNA
-
-
-
?
DNA containing formamidopyrimidine-guanine residues + H2O
4,6-diamino-5-formamidopyrimidine + DNA
-
-
-
?
DNA containing formamidopyrimidine-guanine residues + H2O
4,6-diamino-5-formamidopyrimidine + DNA
-
-
-
?
DNA containing methylated formamidopyrimidine-guanine residues + H2O
2,6-diamino-4-hydroxy-5-(N-methyl)formamidopyrimidine + DNA
-
-
-
?
DNA containing methylated formamidopyrimidine-guanine residues + H2O
2,6-diamino-4-hydroxy-5-(N-methyl)formamidopyrimidine + DNA
-
-
-
?
DNA containing methylated formamidopyrimidine-guanine residues + H2O
2,6-diamino-4-hydroxy-5-(N-methyl)formamidopyrimidine + DNA
-
-
-
?
DNA containing methylated formamidopyrimidine-guanine residues + H2O
2,6-diamino-4-hydroxy-5-(N-methyl)formamidopyrimidine + DNA
-
-
-
?
DNA containing methylated formamidopyrimidine-guanine residues + H2O
2,6-diamino-4-hydroxy-5-(N-methyl)formamidopyrimidine + DNA
-
-
-
?
DNA containing ring-opened N7-methylguanine residues + H2O
2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine + DNA
-
-
-
-
?
DNA containing ring-opened N7-methylguanine residues + H2O
2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine + DNA
-
-
-
?
DNA containing ring-opened N7-methylguanine residues + H2O
2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine + DNA
-
-
-
?
DNA containing ring-opened N7-methylguanine residues + H2O
2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine + DNA
-
-
-
?
DNA containing ring-opened N7-methylguanine residues + H2O
2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine + DNA
-
-
-
-
?
DNA containing ring-opened N7-methylguanine residues + H2O
2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine + DNA
-
-
-
?
DNA containing ring-opened N7-methylguanine residues + H2O
2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine + DNA
-
-
-
-
?
DNA containing ring-opened N7-methylguanine residues + H2O
2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine + DNA
-
enzyme excises the secondary alkylation product of 7-methylguanine Fapy
-
?
DNA containing ring-opened N7-methylguanine residues + H2O
2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine + DNA
-
double-stranded DNA is preferred to single-stranded DNA
-
?
DNA containing ring-opened N7-methylguanine residues + H2O
2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine + DNA
-
dublex oligodeoxynucleotides containing Me-Fapy
-
?
DNA containing ring-opened N7-methylguanine residues + H2O
2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine + DNA
-
comparison of repair activities of human OGG1 and Escherichia coli Fpg, almost no paired base-dependent repair, effect of sequence context on repair efficiency, mechanism via Schiff base intermediate
treatment of me-Fapy with Fpg results in delta-elimination products
?
DNA containing ring-opened N7-methylguanine residues + H2O
2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine + DNA
23-oligomer
-
?
DNA containing ring-opened N7-methylguanine residues + H2O
2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine + DNA
-
less mendatory role of P-2 in 2,6-diamino-4-hydroxy-5-N-methyl-formamidopyrimidine-DNA glycosylase activity
-
-
?
DNA containing ring-opened N7-methylguanine residues + H2O
2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine + DNA
-
the C-8 keto group of 8-oxodeoxyguanine and the carbonyl moiety of formamidopyrimidine enable the enzyme to recognize and bind duplex DNA containing modified bases, mechanism
-
?
DNA containing ring-opened N7-methylguanine residues + H2O
2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine + DNA
-
amino terminal localization of the catalytic site
-
?
DNA containing ring-opened N7-methylguanine residues + H2O
2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine + DNA
-
-
-
-
?
DNA containing ring-opened N7-methylguanine residues + H2O
2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine + DNA
-
enzyme excises the secondary alkylation product of 7-methylguanine Fapy
-
?
DNA containing ring-opened N7-methylguanine residues + H2O
2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine + DNA
-
amino terminal localization of the catalytic site
-
?
DNA containing ring-opened N7-methylguanine residues + H2O
2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine + DNA
-
-
?
DNA containing tetrahydrofuran residues + H2O
tetrahydrofuran + DNA
-
-
-
?
DNA containing tetrahydrofuran residues + H2O
tetrahydrofuran + DNA
-
-
-
-
?
additional information
?
-
Fpg prefers thymine glycol, 5-hydroxycytosine, 5-hydroxyuracil, 5,6-dihydrothymine and 5,6-dihydrouracil, over 7,8-dihydro-8-oxoguanine, the oxidation products of 8-oxoG namely, Gh:C, Sp1:C and Sp2:C are the best substrates for the enzyme, single-stranded DNA is also a substrate, no activity with 8-oxoguanine, Fpg substrate specificity, overview
-
-
?
additional information
?
-
-
Fpg prefers thymine glycol, 5-hydroxycytosine, 5-hydroxyuracil, 5,6-dihydrothymine and 5,6-dihydrouracil, over 7,8-dihydro-8-oxoguanine, the oxidation products of 8-oxoG namely, Gh:C, Sp1:C and Sp2:C are the best substrates for the enzyme, single-stranded DNA is also a substrate, no activity with 8-oxoguanine, Fpg substrate specificity, overview
-
-
?
additional information
?
-
the enzyme does not excise 8-oxoguanine from DNA
-
-
?
additional information
?
-
-
the enzyme does not excise 8-oxoguanine from DNA
-
-
?
additional information
?
-
-
Fpg prefers thymine glycol, 5-hydroxycytosine, 5-hydroxyuracil, 5,6-dihydrothymine and 5,6-dihydrouracil, over 7,8-dihydro-8-oxoguanine, the oxidation products of 8-oxoG namely, Gh:C, Sp1:C and Sp2:C are the best substrates for the enzyme, single-stranded DNA is also a substrate, no activity with 8-oxoguanine, Fpg substrate specificity, overview
-
-
?
additional information
?
-
-
not: carbocyclic substrate analog of 8-oxo-7,8-dihydro-2-deoxyguanosine
-
-
?
additional information
?
-
-
no cleavage of 3-methyladenine, uracil, intact 7-methylguanine from DNA
-
-
?
additional information
?
-
-
not: dublex DNA containing a single tetrahydrofuran residue
-
-
?
additional information
?
-
-
human OGG1 and Escherichia coli Fpg are structurally unrelated enzymes with different catalytic residues, OGG1: Lys-249 and Asp-268 in the hairpin-helix-hairpin-GDP motif are involved in the glycosylase/AP lyase activity, Fpg: uses a proline residue in the N-terminal region for catalysis
-
-
?
additional information
?
-
-
not: DNA containing 8-oxo-7,8-dihydro-2-deoxyadenosine, single-stranded DNA, dublex DNA containing synthetic abasic sites, mismatches containing dG, unmodified DNA
-
-
?
additional information
?
-
-
base excision repair initiated by the enzyme is less effective in the first two days of growth and more effective later in stationary phase
-
-
?
additional information
?
-
-
enzyme mediates repair of lesions containing hydantoins in vivo
-
-
?
additional information
?
-
-
cross-linking of active center with a series of reactive oligonucleotide duplexes containing both a single 8-oxoguanine residue and an O-ethyl-substituted diphosphate internucleotide group results in identification of seven phosphate groups on both strands of the DNA duplex specifically interacting with nucleophilic amino acids of the enzyme. L56 of enzyme cross-links to the phosphate located 3' to the 8-oxoguanine residue
-
-
?
additional information
?
-
-
defective repair of 5-hydroxy-2-deoxycytidine in Cockayne syndrome cells is complementated by Escherichia coli formamidopyrimidine DNA glycosylase and endonuclease III
-
-
?
additional information
?
-
-
formamidopyrimidine-DNA N-glycosylase operates in the base excision repair pathway in bacteria by removing oxidized guanine bases from DNA and can also cleave the nascent or preformed abasic DNA by beta,delta-elimination. The cleaved product formation is initially reversible
-
-
?
additional information
?
-
-
FPG excises oxidatively damaged purines in the base excision repair pathway, overview
-
-
?
additional information
?
-
-
formamidopyrimidine-DNA N-glycosylase removes oxidized guanine bases from DNA. The cleaved product formation is initially reversible, it is followed by conformational changes in the enzyme and DNA molecules that represent the postchemical irreversible rate-limiting steps. The overall rate-limiting step of the enzymatic reaction seems to be the release of Fpg from its adduct with the 4-oxo-2-pentenal remnant of the deoxyribose moiety formed as a result of DNA strand cleavage by beta,delta-elmination, the initial chemical steps are fast and reversible. Catalytic mechanism, overview
-
-
?
additional information
?
-
-
FPG excises oxidatively damaged purines in the base excision repair pathway, it acts on DNA containing 5,6-dihydrouracil, 8-oxo-7,8-dihydroguanine, or on apurinic/apyrimidinic DNA base pairs, analysis of conformational dynamics of Fpg protein and DNA substrates, rate constants of conformational transitions, and intrinsic mechanism of recognition and excision of damaged bases in DNA, overview
-
-
?
additional information
?
-
-
formamidopyrimidine DNA glycosylase is specific for oxidized purines
-
-
?
additional information
?
-
-
the enzyme is more active towards oxidized purines than oxidized pyrimidines and has little to no activity toward DNA containing thymine glycols and osmium-tetroxide-treated DNA
-
-
?
additional information
?
-
-
analysis of acidity and proton affinity for a range of substrates such as 8-oxoguanine, 8-oxoadenine, 8-oxoinosine, 8-oxonebularine, formamidopyrimidine-guanine, 5-hydroxyuracil, and 5,6-dihydrothymine. The most favorable mechanism involves preprotonation of the O4', which results in the opening of the ribose ring
-
-
?
additional information
?
-
-
not: carbocyclic substrate analog of 8-oxo-7,8-dihydro-2-deoxyguanosine
-
-
?
additional information
?
-
-
the enzyme is more active towards oxidized purines than oxidized pyrimidines and has little to no activity toward DNA containing thymine glycols and osmium-tetroxide-treated DNA
-
-
?
additional information
?
-
not: DNA containing 2-hydroxyadenine, 1-N6 ethenoadenine, 3-N4 ethenocytosine, hypoxanthine, xanthine
-
-
?
additional information
?
-
not: DNA containing 2-hydroxyadenine, 1-N6 ethenoadenine, 3-N4 ethenocytosine, hypoxanthine, xanthine
-
-
?
additional information
?
-
-
cross-linking of active center with a series of reactive oligonucleotide duplexes containing both a single 8-oxoguanine residue and an O-ethyl-substituted diphosphate internucleotide group results in identification of eight phosphate groups on both strands of the DNA duplex specifically interacting with nucleophilic amino acids of the enzyme. L249 of enzyme cross-links to the phosphate located 3' to the 8-oxoguanine residue
-
-
?
additional information
?
-
-
treatment of cells with 4-(acetoxymethylnitrosamino)-1-(3-pyridyl)-1-butanone generates formamidopyrimidine glycosylase sensitive DNA sites with cell-type dependent differences in adduct frequency and time
-
-
?
additional information
?
-
8-oxo-7,8-dihydroguanine, i.e. 8-oxoGua, and 2,6-diamino-4-hydroxy-5-formamidopyrimidine, i.e. FapyGua, are premutagenic DNA lesions that appear in DNA damaged by reactive oxygen species of endogenous and environmental origin, and are excised from DNA by the enzyme. The fidelity of the 8-oxoGua repair system depends on discrimination between 8-oxoGua:Cyt and 8-oxoGua:Ade pairs by OGG1
-
-
?
additional information
?
-
-
8-oxo-7,8-dihydroguanine, i.e. 8-oxoGua, and 2,6-diamino-4-hydroxy-5-formamidopyrimidine, i.e. FapyGua, are premutagenic DNA lesions that appear in DNA damaged by reactive oxygen species of endogenous and environmental origin, and are excised from DNA by the enzyme. The fidelity of the 8-oxoGua repair system depends on discrimination between 8-oxoGua:Cyt and 8-oxoGua:Ade pairs by OGG1
-
-
?
additional information
?
-
-
NEIL1 is active on DNA lesions in ssDNA, particularly in the context of a single-stranded bubble in a duplex sequence
-
-
?
additional information
?
-
8-oxoguanine-DNA glycosylase, OGG1, efficiently removes mutagenic 8-oxo-7,8-dihydroguanine and 2,6-diamino-4-hydroxy-5-formamidopyrimidine when paired with cytosine in oxidatively damaged DNA. Excision of 8-oxoGua mispaired with adenine may lead to G to T transversions. Substrate specificity of wild-type and mutant enzymes, overview
-
-
?
additional information
?
-
-
8-oxoguanine-DNA glycosylase, OGG1, efficiently removes mutagenic 8-oxo-7,8-dihydroguanine and 2,6-diamino-4-hydroxy-5-formamidopyrimidine when paired with cytosine in oxidatively damaged DNA. Excision of 8-oxoGua mispaired with adenine may lead to G to T transversions. Substrate specificity of wild-type and mutant enzymes, overview
-
-
?
additional information
?
-
-
the enzyme is a DNA glycosylase/AP lyase specific for oxidized purines such as 8-oxo-7,8-dihydropurines and imidazole-ring opened purines (2,6-diamino-4-hydroxy-5-formamidopyrimidine and formamidopyrimidine derived from adenine)
-
-
?
additional information
?
-
-
MmuNeil3 is a bifunctional DNA glycosylase that recognizes spiroiminodihydantoin and guanidinohydantoin, as well as 2,6-diamino-4-hydroxy-5-formamidopyrimidine, and 4,6-diamino-5-formamidopyrimidine, in double-stranded substrates. Neil3 greatly reduces both the spontaneous mutation frequency and the level of 2,6-diamino-4-hydroxy-5-formamidopyrimidine in the DNA. Substrate specificity of MmuNeil3 in vivo, overview
-
-
?
additional information
?
-
-
NEIL1 is active on DNA lesions in ssDNA, particularly in the context of a single-stranded bubble in a duplex sequence
-
-
?
additional information
?
-
-
in duplex DNA, mouse Neil3 recognizes the oxidized purines, spiroiminodihydantoin, guanidinohydantoin, 2,6-diamino-4-hydroxy-5-formamidopyrimidine, and 4,6-diamino-5-formamidopyrimidine, but not 8-oxo-7,8-dihydroguanine. Neil3 prefers lesions in single-stranded DNA and in bubble structures. Mouse Neil3 forms a Schiff base intermediate via its N-terminal valine, in contrast to other enzyme of the family that use proline as nucleophile
-
-
?
additional information
?
-
-
the enzyme has no specificity for DNA containing 8-hydroxyguanine residues
-
-
?
additional information
?
-
the mouse enzyme cannot initiate repair of aflatoxin B1-induced DNA base alkylation damage in either ds or ss DNA
-
-
?
additional information
?
-
-
the mouse enzyme cannot initiate repair of aflatoxin B1-induced DNA base alkylation damage in either ds or ss DNA
-
-
?
additional information
?
-
Mtb-Fpg1 removes formamidopyrimidine and 5-hydroxycytosine lesions, as well as 8-oxo-7,8-dihydroguanine opposite to C, T and G. Substrates are duplex DNA substrates containing a single 8oxoG opposite of C, A, G, T, 5-hydroxycytosine (5OHC):G, 5-hydroxyuracil (5OHU):G, DHU:G, U:A or U:G base pair
-
-
?
additional information
?
-
-
Mtb-Fpg1 removes formamidopyrimidine and 5-hydroxycytosine lesions, as well as 8-oxo-7,8-dihydroguanine opposite to C, T and G. Substrates are duplex DNA substrates containing a single 8oxoG opposite of C, A, G, T, 5-hydroxycytosine (5OHC):G, 5-hydroxyuracil (5OHU):G, DHU:G, U:A or U:G base pair
-
-
?
additional information
?
-
Mtb-Fpg1 removes formamidopyrimidine and 5-hydroxycytosine lesions, as well as 8-oxo-7,8-dihydroguanine opposite to C, T and G. Substrates are duplex DNA substrates containing a single 8oxoG opposite of C, A, G, T, 5-hydroxycytosine (5OHC):G, 5-hydroxyuracil (5OHU):G, DHU:G, U:A or U:G base pair
-
-
?
additional information
?
-
-
Mtb-Fpg1 removes formamidopyrimidine and 5-hydroxycytosine lesions, as well as 8-oxo-7,8-dihydroguanine opposite to C, T and G. Substrates are duplex DNA substrates containing a single 8oxoG opposite of C, A, G, T, 5-hydroxycytosine (5OHC):G, 5-hydroxyuracil (5OHU):G, DHU:G, U:A or U:G base pair
-
-
?
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0.0000039 - 0.0000466
13mer oligonucleotide duplex containing 8-oxoguanine
-
0.0000063 - 0.000018
23mer oligonucleotide duplex containing 8-oxoguanine
-
0.00015
5,6-dihydrouracil-DNA
-
NEIL1
-
0.0000034 - 0.00001
8-oxo-7,8-dihydroguanine:Cyt oligodeoxynucleotide
-
0.00178 - 0.00487
DNA containing 2,6-diamino-4-hydroxy-5-formamidopyrimidine residues
-
0.00078 - 0.00129
DNA containing 4,6-diamino-5-formamidopyrimidine residues
-
0.00045
DNA containing 5,6-dihydrothymine residues
-
pH 7.5, 37°C
-
0.00061 - 0.00957
DNA containing 5,6-dihydrouracil
0.0047
DNA containing 5-hydroxycytosine residues
-
pH 7.5, 37°C
-
0.000083 - 0.000126
DNA containing 7-deaza-2'-deoxyguanosine
0.0000069 - 0.002
DNA containing 7-hydro-8-oxoguanine residues
-
0.000053 - 0.000137
DNA containing 7-methyl-8-oxo-2'-deoxyguanosine
0.000004 - 0.00311
DNA containing 8-hydroxyguanine residues
-
0.000012
DNA containing 8-oxo-2'-deoxyguanosine
0.001 - 0.44
DNA containing 8-oxo-guanine residues
-
0.022 - 0.023
DNA containing 8-oxo-guanine residues mispaired to adenine
-
0.0001 - 0.0051
DNA containing 8-oxo-guanine residues mispaired to guanine
-
0.00019 - 0.0042
DNA containing 8-oxo-guanine residues mispaired to thymine
-
0.000151 - 0.000225
DNA containing 8-thio-2'-deoxyguanosine
0.000009 - 0.000041
DNA containing ring-opened N7-methylguanine residues
-
additional information
additional information
-
0.0000039
13mer oligonucleotide duplex containing 8-oxoguanine
mutant R244E, pH 7.5, 37°C
-
0.0000047
13mer oligonucleotide duplex containing 8-oxoguanine
mutant S208A, pH 7.5, 37°C
-
0.000006
13mer oligonucleotide duplex containing 8-oxoguanine
mutant Q234R, pH 7.5, 37°C
-
0.0000081
13mer oligonucleotide duplex containing 8-oxoguanine
wild-type, pH 7.5, 37°C
-
0.0000088
13mer oligonucleotide duplex containing 8-oxoguanine
mutant Q234R/R244E, pH 7.5, 37°C
-
0.0000181
13mer oligonucleotide duplex containing 8-oxoguanine
-
15°C
-
0.0000222
13mer oligonucleotide duplex containing 8-oxoguanine
-
17.5°C
-
0.0000296
13mer oligonucleotide duplex containing 8-oxoguanine
-
20°C
-
0.0000369
13mer oligonucleotide duplex containing 8-oxoguanine
-
22.5°C
-
0.0000466
13mer oligonucleotide duplex containing 8-oxoguanine
-
25°C
-
0.0000063
23mer oligonucleotide duplex containing 8-oxoguanine
mutant R258Q, pH 7.5, 30°C
-
0.0000081
23mer oligonucleotide duplex containing 8-oxoguanine
wild-type, pH 7.5, 30°C
-
0.0000081
23mer oligonucleotide duplex containing 8-oxoguanine
mutant N168Q, pH 7.5, 30°C
-
0.000018
23mer oligonucleotide duplex containing 8-oxoguanine
mutant R108K, pH 7.5, 30°C
-
0.0000034
8-oxo-7,8-dihydroguanine:Cyt oligodeoxynucleotide
pH 7.4-7.5, mutant S326C
-
0.0000034
8-oxo-7,8-dihydroguanine:Cyt oligodeoxynucleotide
pH 7.4-7.5, wild-type enzyme
-
0.0000057
8-oxo-7,8-dihydroguanine:Cyt oligodeoxynucleotide
pH 7.4-7.5, mutant S231E
-
0.0000061
8-oxo-7,8-dihydroguanine:Cyt oligodeoxynucleotide
pH 7.4-7.5, mutant D322N
-
0.0000074
8-oxo-7,8-dihydroguanine:Cyt oligodeoxynucleotide
pH 7.4-7.5, mutant S280E
-
0.0000075
8-oxo-7,8-dihydroguanine:Cyt oligodeoxynucleotide
pH 7.4-7.5, mutant S326E
-
0.0000086
8-oxo-7,8-dihydroguanine:Cyt oligodeoxynucleotide
pH 7.4-7.5, mutant A288V
-
0.0000092
8-oxo-7,8-dihydroguanine:Cyt oligodeoxynucleotide
pH 7.4-7.5, mutant S232E
-
0.00001
8-oxo-7,8-dihydroguanine:Cyt oligodeoxynucleotide
pH 7.4-7.5, mutant S231E/S232E
-
0.0000009
DNA
pH 7.5, 20°C, 24-oligomer DNA containing apurinic/apyrimidinic sites, lyase activity, K155A mutant Fpg
0.000019
DNA
pH 7.5, 20°C, 24-oligomer DNA containing AP sites, lyase activity, wild-type Fpg
0.0046
DNA
-
pH 7.5, 25°C, DNA containing an apurinic/apyrimidinic site
0.00178
DNA containing 2,6-diamino-4-hydroxy-5-formamidopyrimidine residues
-
pH 7.4, 37°C, K57R mutant Fpg, from DNA gamma-irradiated under NO2
-
0.00187
DNA containing 2,6-diamino-4-hydroxy-5-formamidopyrimidine residues
-
pH 7.4, 37°C, K57G mutant Fpg, from DNA gamma-irradiated under NO2
-
0.00343 - 0.00376
DNA containing 2,6-diamino-4-hydroxy-5-formamidopyrimidine residues
-
pH 7.4, 37°C, wild-type Fpg
-
0.00422
DNA containing 2,6-diamino-4-hydroxy-5-formamidopyrimidine residues
-
pH 7.4, 37°C, K57G mutant Fpg, from DNA treated with H2O2/Fe(III)-EDTA/asc
-
0.00487
DNA containing 2,6-diamino-4-hydroxy-5-formamidopyrimidine residues
-
pH 7.4, 37°C, K57R mutant Fpg, from DNA treated with H2O2/Fe(III)-EDTA/asc
-
0.00078 - 0.00084
DNA containing 4,6-diamino-5-formamidopyrimidine residues
-
pH 7.4, 37°C, K57G mutant Fpg
-
0.00079 - 0.0009
DNA containing 4,6-diamino-5-formamidopyrimidine residues
-
pH 7.4, 37°C, K57R mutant Fpg
-
0.00107 - 0.00129
DNA containing 4,6-diamino-5-formamidopyrimidine residues
-
pH 7.4, 37°C, wild-type Fpg
-
0.00061
DNA containing 5,6-dihydrouracil
-
mutant K217A
0.00068
DNA containing 5,6-dihydrouracil
-
wild-type
0.0007
DNA containing 5,6-dihydrouracil
-
mutant H89A
0.0007
DNA containing 5,6-dihydrouracil
-
mutant R108A
0.00465
DNA containing 5,6-dihydrouracil
-
mutant R109A
0.00957
DNA containing 5,6-dihydrouracil
-
mutant H89A/R109A
0.000083
DNA containing 7-deaza-2'-deoxyguanosine
-
-
0.000126
DNA containing 7-deaza-2'-deoxyguanosine
-
-
0.0000069
DNA containing 7-hydro-8-oxoguanine residues
pH 7.5, 37°C, 24-oligomer, wild-type Fpg
-
0.0000075
DNA containing 7-hydro-8-oxoguanine residues
pH 7.5, 37°C, 24-oligomer, K155A mutant Fpg
-
0.002
DNA containing 7-hydro-8-oxoguanine residues
-
pH 7.5, 25°C
-
0.000053
DNA containing 7-methyl-8-oxo-2'-deoxyguanosine
-
-
0.000137
DNA containing 7-methyl-8-oxo-2'-deoxyguanosine
-
-
0.000004
DNA containing 8-hydroxyguanine residues
-
pH 7.8, 37°C, 34-oligomer, wild-type and K57R mutant Fpg
-
0.000008
DNA containing 8-hydroxyguanine residues
-
pH 7.8, 37°C, 34-oligomer, K57G mutant Fpg
-
0.000008
DNA containing 8-hydroxyguanine residues
-
pH 7.5, 20°C, dublex DNA containing a single 8-oxoguanine residue positioned opposite dC
-
0.000013
DNA containing 8-hydroxyguanine residues
-
pH 7.5, 37°C
-
0.00093 - 0.00155
DNA containing 8-hydroxyguanine residues
-
pH 7.4, 37°C, K57R mutant Fpg
-
0.00109
DNA containing 8-hydroxyguanine residues
-
pH 7.4, 37°C, wild-type Fpg, from DNA treated with H2O2/Fe(III)-EDTA/asc
-
0.00126
DNA containing 8-hydroxyguanine residues
-
pH 7.4, 37°C, K57G mutant Fpg
-
0.00311
DNA containing 8-hydroxyguanine residues
-
pH 7.4, 37°C, wild-type Fpg, from DNA gamma-irradiated under NO2
-
0.000012
DNA containing 8-oxo-2'-deoxyguanosine
-
-
0.000012
DNA containing 8-oxo-2'-deoxyguanosine
-
-
0.001
DNA containing 8-oxo-guanine residues
-
wild-type
-
0.0015
DNA containing 8-oxo-guanine residues
-
mutant R108A
-
0.0041
DNA containing 8-oxo-guanine residues
-
mutant H89A
-
0.0041
DNA containing 8-oxo-guanine residues
-
mutant K217T
-
0.227
DNA containing 8-oxo-guanine residues
-
mutant R109A
-
0.44
DNA containing 8-oxo-guanine residues
-
mutant H89A/R109A
-
0.022
DNA containing 8-oxo-guanine residues mispaired to adenine
-
wild-type
-
0.023
DNA containing 8-oxo-guanine residues mispaired to adenine
-
mutant R108A
-
0.0001
DNA containing 8-oxo-guanine residues mispaired to guanine
-
wild-type
-
0.0051
DNA containing 8-oxo-guanine residues mispaired to guanine
-
mutant R108A
-
0.00019
DNA containing 8-oxo-guanine residues mispaired to thymine
-
wild-type
-
0.0042
DNA containing 8-oxo-guanine residues mispaired to thymine
-
mutant R108A
-
0.000151
DNA containing 8-thio-2'-deoxyguanosine
-
-
0.000225
DNA containing 8-thio-2'-deoxyguanosine
-
-
0.000009 - 0.000011
DNA containing ring-opened N7-methylguanine residues
-
pH 7.8, 37°C, wild-type, K57G and K57R mutant Fpg
-
0.000023
DNA containing ring-opened N7-methylguanine residues
pH 7.5, 37°C, 23-oligomer, wild-type Fpg
-
0.000029
DNA containing ring-opened N7-methylguanine residues
pH 7.5, 37°C, 23-oligomer, K155A mutant Fpg
-
0.000038
DNA containing ring-opened N7-methylguanine residues
-
pH 7.5, 37°C
-
0.000041
DNA containing ring-opened N7-methylguanine residues
-
pH 7.5, 20°C, dublex DNA containing a single Me-Fapy residue positioned opposite dC
-
additional information
additional information
-
Km values for excision of purine lesions from DNA treated with various free radical-generating systems
-
additional information
additional information
-
thermodynamic characterization of Fpg binding to lesion-containing 13mer DNA dublexes, binding affinity
-
additional information
additional information
-
Km for DNA substrate is affected by the base opposite the lesion
-
additional information
additional information
-
kinetic parameters of duplex oligonucleotides and mismatched duplexes
-
additional information
additional information
-
steady state kinetics
-
additional information
additional information
-
steady-state kinetics, overview
-
additional information
additional information
-
Fpg single-turnover kinetics, overview
-
additional information
additional information
Fpg single-turnover kinetics, overview
-
additional information
additional information
-
Fpg single-turnover kinetics, overview
-
additional information
additional information
-
kinetics, detailed overview
-
additional information
additional information
-
transient kinetics of Fpg
-
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Boiteux, S.; O'Connor, T.R.; Laval, J.
Formamidopyrimidine-DNA glycosylase of Escherichia coli: cloning and sequencing of the fpg structural gene and overproduction of the protein
EMBO J.
6
3177-3183
1987
Escherichia coli, Escherichia coli HB1100
brenda
Tchou, J.; Grollman, A.P.
The catalytic mechanism of Fpg protein. Evidence for a Schiff base intermediate and amino terminus localization of the catalytic site
J. Biol. Chem.
270
11671-11677
1995
Escherichia coli, Escherichia coli JM109
brenda
Tchou, J.; Bodepudi, V.; Shibutani, S.; Antoshechkin, I.; Miller, J.; Grollman, A.P.; Johnson, F.
Substrate specificity of Fpg protein. Recognition and cleavage of oxidatively damaged DNA
J. Biol. Chem.
269
15318-15324
1994
Escherichia coli
brenda
Tchou, J.; Kasai, H.; Chung, M.H.; Laval, J.; Grollman, A.P.; Nishimura, S.
8-Oxoguanine (8-hydroxyguanine) DNA glycosylase and its substrate specificity
Proc. Natl. Acad. Sci. USA
88
4690-4694
1991
Escherichia coli
brenda
O'Connor, T.R.; Laval, J.
Physical association of the 2,6-diamino-4-hydroxy-5N-formamidopyrimidine-DNA glycosylase of Escherichia coli and an activity nicking DNA at apurinic/apyrimidinic sites
Proc. Natl. Acad. Sci. USA
86
5222-5226
1989
Escherichia coli
brenda
Bailly, V.; Verly, W.G.; O'Connor, T.; Laval, J.
Mechanism of DNA strand nicking at apurinic/apyrimidinic sites by Escherichia coli [formamidopyrimidine]DNA glycosylase
Biochem. J.
262
581-589
1989
Escherichia coli
brenda
Chetsanga, C.J.; Lozon, M.; Makaroff, C.; Savage, L.
Purification and characterization of Escherichia coli formamidopyrimidine-DNA glycosylase that excises damaged 7-methylguanine from deoxyribonucleic acid
Biochemistry
20
5201-5207
1981
Escherichia coli
brenda
Hatahet, Z.; Kow, Y.W.; Purmal, A.A.; Cunningham, R.P.; Wallace, S.S.
New substrates for old enzymes. 5-Hydroxy-2'-deoxycytidine and 5-hydroxy-2'-deoxyuridine are substrates for Escherichia coli endonuclease III and formamidopyrimidine DNA N-glycosylase, while 5-hydroxy-2'-deoxyuridine is a substrate for uracil DNA N-glycosylase
J. Biol. Chem.
269
18814-18820
1994
Escherichia coli
brenda
Serre, L.; Pereira de Jesus, K.; Boiteux, S.; Zelwer, C.; Castaing, B.
Crystal structure of the Lactococcus lactis formamidopyrimidine-DNA glycosylase bound to an abasic site analogue-containing DNA
EMBO J.
21
2854-2865
2002
Lactococcus lactis
brenda
Gilboa, R.; Zharkov, D.O.; Golan, G.; Fernandes, A.S.; Gerchman, S.E.; Matz, E.; Kycia, J.H.; Grollman, A.P.; Shoham, G.
Structure of formamidopyrimidine-DNA glycosylase covalently complexed to DNA
J. Biol. Chem.
277
19811-19816
2002
Escherichia coli
brenda
Buchko, G.W.; Hess, N.J.; Bandaru, V.; Wallace, S.S.; Kennedy, M.A.
Spectroscopic studies of zinc(II)- and cobalt(II)-associated Escherichia coli formamidopyrimidine-DNA glycosylase: extended X-ray absorption fine structure evidence for a metal-binding domain
Biochemistry
39
12441-12449
2000
Escherichia coli
brenda
Duwat, P.; de Oliveira, R.; Ehrlich, S.D.; Boiteux, S.
Repair of oxidative DNA damage in gram-positive bacteria: the Lactococcus lactis Fpg protein
Microbiology
141
411-417
1995
Lactococcus lactis (P42371), Lactococcus lactis
brenda
Kuznetsov, S.V.; Sidorkina, O.M.; Jurado, J.; Bazin, M.; Tauc, P.; Brochon, J.C.; Laval, J.; Santus, R.
Effect of single mutations on the structural dynamics of a DNA repair enzyme, the Escherichia coli formamidopyrimidine-DNA glycosylase. A fluorescence study using tryptophan residues as reporter groups
Eur. J. Biochem.
253
413-420
1998
Escherichia coli, Escherichia coli BH20
brenda
Sidorkina, O.; Dizdaroglu, M.; Laval, J.
Effect of single mutations on the specificity of Escherichia coli FPG protein for excision of purine lesions from DNA damaged by free radicals
Free Radic. Biol. Med.
31
816-823
2001
Escherichia coli
brenda
Rabow, L.E.; Kow, Y.W.
Mechanism of action of base release by Escherichia coli Fpg protein: Role of lysine 155 in catalysis
Biochemistry
36
5084-5096
1997
Escherichia coli (P50465), Escherichia coli
brenda
Rabow, L.; Venkataraman, R.; Kow, Y.W.
Mechanism of action of Escherichia coli formamidopyrimidine N-glycosylase: Role of K155 in substrate binding and product release
Prog. Nucleic Acid Res. Mol. Biol.
68
223-234
2001
Escherichia coli
brenda
Gao, M.J.; Murphy, T.M.
Alternative forms of formamidopyrimidine-DNA glycosylase from Arabidopsis thaliana
Photochem. Photobiol.
73
128-134
2001
Arabidopsis thaliana (O80358), Arabidopsis thaliana
brenda
D'Ham, C.; Romieu, A.; Jaquinod, M.; Gasparutto, D.; Cadet, J.
Excision of 5,6-dihydroxy-5,6-dihydrothymine, 5,6-dihydrothymine, and 5-hydroxycytosine from defined sequence oligonucleotides by Escherichia coli endonuclease III and Fpg proteins: Kinetic and mechanistic aspects
Biochemistry
38
3335-3344
1999
Escherichia coli
brenda
Sentuerker, S.; Bauche, C.; Laval, J.; Dizdaroglu, M.
Substrate specificity of Deinococcus radiodurans Fpg protein
Biochemistry
38
9435-9439
1999
Deinococcus radiodurans
brenda
Sidorkina, O.M.; Laval, J.
Role of lysine-57 in the catalytic activities of Escherichia coli formamidopyrimidine-DNA glycosylase (Fpg protein)
Nucleic Acids Res.
26
5351-5357
1998
Escherichia coli, Escherichia coli BH20
brenda
Minetti, C.A.S.A.; Remeta, D.P.; Zharkov, D.O.; Plum, G.E.; Johnson, F.; Grollman, A.P.; Breslauer, K.J.
Energetics of lesion recognition by a DNA repair protein: Thermodynamic characterization of formamidopyrimidine-glycosylase (Fpg) interactions with damaged DNA duplexes
J. Mol. Biol.
328
1047-1060
2003
Escherichia coli, Escherichia coli B834 (DE3)
brenda
Fedorova, O.S.; Nevinsky, G.A.; Koval, V.V.; Ishchenko, A.A.; Vasilenko, N.L.; Douglas, K.T.
Stopped-flow kinetic studies of the interaction between Escherichia coli Fpg protein and DNA substrates
Biochemistry
41
1520-1528
2002
Escherichia coli
brenda
Asagoshi, K.; Yamada, T.; Terato, H.; Ohyama, Y.; Monden, Y.; Arai, T.; Nishimura, S.; Aburatani, H.; Lindahl, T.; Ide, H.
Distinct repair activities of human 7,8-dihydro-8-oxoguanine DNA glycosylase and formamidopyrimidine DNA glycosylase for formamidopyrimidine and 7,8-dihydro-8-oxoguanine
J. Biol. Chem.
275
4956-4964
2000
Escherichia coli
brenda
Sidorkina, O.M.; Laval, J.
Role of the N-terminal proline residue in the catalytic activities of the Escherichia coli Fpg protein
J. Biol. Chem.
275
9924-9929
2000
Escherichia coli
brenda
Hazra, T.K.; Izumi, T.; Boldogh, I.; Imhoff, B.; Kow, Y.W.; Jaruga, P.; Dizdaroglu, M.; Mitra, S.
Identification and characterization of a human DNA glycosylase for repair of modified bases in oxidatively damaged DNA
Proc. Natl. Acad. Sci. USA
99
3523-3528
2002
Homo sapiens (Q969S2), Homo sapiens (Q96FI4)
brenda
Murphy, T.M.; George, A.
A comparison of two DNA base excision repair glycosylases from Arabidopsis thaliana
Biochem. Biophys. Res. Commun.
329
869-872
2005
Arabidopsis thaliana
brenda
Perlow-Poehnelt, R.A.; Zharkov, D.O.; Grollman, A.P.; Broyde, S.
Substrate discrimination by formamidopyrimidine-DNA glycosylase: distinguishing interactions within the active site
Biochemistry
43
16092-16105
2004
Escherichia coli (P05523), Escherichia coli
brenda
Wozniak, K.; Blasiak, J.
Nickel impairs the repair of UV- and MNNG-damaged DNA
Cell. Mol. Biol. Lett.
9
83-94
2004
Homo sapiens
brenda
Buchko, G.W.; McAteer, K.; Wallace, S.S.; Kennedy, M.A.
Solution-state NMR investigation of DNA binding interactions in Escherichia coli formamidopyrimidine-DNA glycosylase (Fpg): a dynamic description of the DNA/protein interface
DNA Repair
4
327-339
2005
Escherichia coli
brenda
Zaika, E.I.; Perlow, R.A.; Matz, E.; Broyde, S.; Gilboa, R.; Grollman, A.P.; Zharkov, D.O.
Substrate discrimination by formamidopyrimidine-DNA glycosylase: a mutational analysis
J. Biol. Chem.
279
4849-4861
2004
Escherichia coli
brenda
Koval, V.V.; Kuznetsov, N.A.; Zharkov, D.O.; Ishchenko, A.A.; Douglas, K.T.; Nevinsky, G.A.; Fedorova, O.S.
Pre-steady-state kinetics shows differences in processing of various DNA lesions by Escherichia coli formamidopyrimidine-DNA glycosylase
Nucleic Acids Res.
32
926-935
2004
Escherichia coli
brenda
Reddy, P.; Jaruga, P.; O'Connor, T.; Rodriguez, H.; Dizdaroglu, M.
Overexpression and rapid purification of Escherichia coli formamidopyrimidine-DNA glycosylase
Protein Expr. Purif.
34
126-133
2004
Escherichia coli
brenda
Amara, P.; Serre, L.; Castaing, B.; Thomas, A.
Insights into the DNA repair process by the formamidopyrimidine-DNA glycosylase investigated by molecular dynamics
Protein Sci.
13
2009-2021
2004
Geobacillus stearothermophilus, Lactococcus lactis
brenda
Song, K.; Hornak, V.; de Los Santos, C.; Grollman, A.P.; Simmerling, C.
Computational analysis of the mode of binding of 8-oxoguanine to formamidopyrimidine-DNA glycosylase
Biochemistry
45
10886-10894
2006
Geobacillus stearothermophilus (P84131), Geobacillus stearothermophilus
brenda
Harbut, M.B.; Meador, M.; Dodson, M.L.; Lloyd, R.S.
Modulation of the turnover of formamidopyrimidine DNA glycosylase
Biochemistry
45
7341-7346
2006
Escherichia coli (P05523), Escherichia coli
brenda
Kuznetsov, N.A.; Koval, V.V.; Zharkov, D.O.; Vorobjev, Y.N.; Nevinsky, G.A.; Douglas, K.T.; Fedorova, O.S.
Pre-steady-state kinetic study of substrate specificity of Escherichia coli formamidopyrimidine--DNA glycosylase
Biochemistry
46
424-435
2007
Escherichia coli (P05523), Escherichia coli
brenda
Krishnamurthy, N.; Muller, J.G.; Burrows, C.J.; David, S.S.
Unusual structural features of hydantoin lesions translate into efficient recognition by Escherichia coli Fpg
Biochemistry
46
9355-9365
2007
Escherichia coli
brenda
Jain, R.; Kumar, P.; Varshney, U.
A distinct role of formamidopyrimidine DNA glycosylase (MutM) in down-regulation of accumulation of G, C mutations and protection against oxidative stress in mycobacteria
DNA Repair
6
1774-1785
2007
Mycolicibacterium smegmatis
brenda
Paul, S.; Gros, L.; Laval, J.; Sutherland, B.M.
Expression of the E. coli fpg protein in CHO cells lowers endogenous oxypurine clustered damage levels and decreases accumulation of endogenous Hprt mutations
Environ. Mol. Mutagen.
47
311-319
2006
Escherichia coli
brenda
Ropolo, M.; Degan, P.; Foresta, M.; DErrico, M.; Lasiglie, D.; Dogliotti, E.; Casartelli, G.; Zupo, S.; Poggi, A.; Frosina, G.
Complementation of the oxidatively damaged DNA repair defect in Cockayne syndrome A and B cells by Escherichia coli formamidopyrimidine DNA glycosylase
Free Radic. Biol. Med.
42
1807-1817
2007
Escherichia coli
brenda
Ropolo, M.; Geroldi, A.; Degan, P.; Andreotti, V.; Zupo, S.; Poggi, A.; Reed, A.; Kelley, M.R.; Frosina, G.
Accelerated repair and reduced mutagenicity of oxidative DNA damage in human bladder cells expressing the E. coli FPG protein
Int. J. Cancer
118
1628-1634
2006
Escherichia coli
brenda
Song, K.; Kelso, C.; de los Santos, C.; Grollman, A.P.; Simmerling, C.
Molecular simulations reveal a common binding mode for glycosylase binding of oxidatively damaged DNA lesions
J. Am. Chem. Soc.
129
14536-14537
2007
Geobacillus stearothermophilus
brenda
Hamm, M.L.; Gill, T.J.; Nicolson, S.C.; Summers, M.R.
Substrate specificity of Fpg (MutM) and hOGG1, two repair glycosylases
J. Am. Chem. Soc.
129
7724-7725
2007
Escherichia coli, Homo sapiens
brenda
Rogacheva, M.; Ishchenko, A.; Saparbaev, M.; Kuznetsova, S.; Ogryzko, V.
High resolution characterization of formamidopyrimidine-DNA glycosylase interaction with its substrate by chemical cross-linking and mass spectrometry using substrate analogs
J. Biol. Chem.
281
32353-32365
2006
Escherichia coli, Homo sapiens
brenda
Smith, C.C.; ODonovan, M.R.; Martin, E.A.
hOGG1 recognizes oxidative damage using the comet assay with greater specificity than FPG or ENDOIII
Mutagenesis
21
185-190
2006
Escherichia coli, Homo sapiens
brenda
Dusinska, M.; Dzupinkova, Z.; Wsolova, L.; Harrington, V.; Collins, A.R.
Possible involvement of XPA in repair of oxidative DNA damage deduced from analysis of damage, repair and genotype in a human population study
Mutagenesis
21
205-211
2006
Homo sapiens
brenda
Shu, J.; Schellhorn, H.E.; Murphy, T.M.
Stationary phase-induction of G->T mutations in Escherichia coli
Mutat. Res.
596
106-112
2006
Escherichia coli
brenda
Poplawski, T.; Arabski, M.; Kozirowska, D.; Blasinska-Morawiec, M.; Morawiec, Z.; Morawiec-Bajda, A.; Klupinska, G.; Jeziorski, A.; Chojnacki, J.; Blasiak, J.
DNA damage and repair in gastric cancer - A correlation with the hOGG1 and RAD51 genes polymorphisms
Mutat. Res.
601
83-91
2006
Homo sapiens
brenda
Lacoste, S.; Castonguay, A.; Drouin, R.
Repair kinetics of specific types of nitroso-induced DNA damage using the comet assay in human cells
Mutat. Res.
624
18-30
2007
Homo sapiens
brenda
Sliwinski, T.; Rozej, W.; Morawiec-Bajda, A.; Morawiec, Z.; Reiter, R.; Blasiak, J.
Protective action of melatonin against oxidative DNA damage-Chemical inactivation versus base-excision repair
Mutat. Res.
634
220-227
2007
Homo sapiens
brenda
Morawiec, Z.; Janik, K.; Kowalski, M.; Stetkiewicz, T.; Szaflik, J.; Morawiec-Bajda, A.; Sobczuk, A.; Blasiak, J.
DNA damage and repair in children with Downs syndrome
Mutat. Res.
637
118-123
2008
Homo sapiens
brenda
Minetti, C.A.; Remeta, D.P.; Breslauer, K.J.
A continuous hyperchromicity assay to characterize the kinetics and thermodynamics of DNA lesion recognition and base excision
Proc. Natl. Acad. Sci. USA
105
70-75
2008
Escherichia coli
brenda
Martinez-Alfaro, M.; Palma-Tirado, L.; Sandoval-Zapata, F.; Carabez-Trejo, A.
Correlation between formamidopyrimidine DNA glycosylase (Fpg)-sensitive sites determined by a comet assay, increased MDA, and decreased glutathione during long exposure to thinner inhalation
Toxicol. Lett.
163
198-205
2006
Rattus norvegicus
brenda
Fracasso, M.E.; Doria, D.; Franceschetti, P.; Perbellini, L.; Romeo, L.
DNA damage and repair capacity by comet assay in lymphocytes of white-collar active smokers and passive smokers (non- and ex-smokers) at workplace
Toxicol. Lett.
167
131-141
2006
Homo sapiens
brenda
Murphy, T.M.; Guo, Y.Y.
Antimutagenic specificities of two plant glycosylases, oxoguanine glycosylase and formamidopyrimidine glycosylase, assayed in vivo
Biochem. Biophys. Res. Commun.
392
335-339
2010
Arabidopsis thaliana (O80358), Arabidopsis thaliana
brenda
Grin, I.R.; Konorovsky, P.G.; Nevinsky, G.A.; Zharkov, D.O.
Heavy metal ions affect the activity of DNA glycosylases of the fpg family
Biochemistry (Moscow)
74
1253-1259
2009
Homo sapiens
brenda
Kuznetsov, N.A.; Zharkov, D.O.; Koval, V.V.; Buckle, M.; Fedorova, O.S.
Reversible chemical step and rate-limiting enzyme regeneration in the reaction catalyzed by formamidopyrimidine-DNA glycosylase
Biochemistry
48
11335-11343
2009
Escherichia coli
brenda
Kathe, S.D.; Barrantes-Reynolds, R.; Jaruga, P.; Newton, M.R.; Burrows, C.J.; Bandaru, V.; Dizdaroglu, M.; Bond, J.P.; Wallace, S.S.
Plant and fungal Fpg homologs are formamidopyrimidine DNA glycosylases but not 8-oxoguanine DNA glycosylases
DNA Repair
8
643-653
2009
Candida albicans, Arabidopsis thaliana (Q88AH6), Arabidopsis thaliana
brenda
Chan, M.K.; Ocampo-Hafalla, M.T.; Vartanian, V.; Jaruga, P.; Kirkali, G.; Koenig, K.L.; Brown, S.; Lloyd, R.S.; Dizdaroglu, M.; Teebor, G.W.
Targeted deletion of the genes encoding NTH1 and NEIL1 DNA N-glycosylases reveals the existence of novel carcinogenic oxidative damage to DNA
DNA Repair
8
786-794
2009
Mus musculus, Mus musculus C57BL/6
brenda
Sidorenko, V.S.; Grollman, A.P.; Jaruga, P.; Dizdaroglu, M.; Zharkov, D.O.
Substrate specificity and excision kinetics of natural polymorphic variants and phosphomimetic mutants of human 8-oxoguanine-DNA glycosylase
FEBS J.
276
5149-5162
2009
Homo sapiens (O15527), Homo sapiens
brenda
Olsen, I.; Balasingham, S.V.; Davidsen, T.; Debebe, E.; Rodland, E.A.; van Soolingen, D.; Kremer, K.; Alseth, I.; Tonjum, T.
Characterization of the major formamidopyrimidine-DNA glycosylase homolog in Mycobacterium tuberculosis and its linkage to variable tandem repeats
FEMS Immunol. Med. Microbiol.
56
151-161
2009
Mycobacterium tuberculosis (P9WNC3), Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv (P9WNC3), Mycobacterium tuberculosis H37Rv
brenda
Foresta, M.; Ropolo, M.; Degan, P.; Pettinati, I.; Kow, Y.W.; Damonte, G.; Poggi, A.; Frosina, G.
Defective repair of 5-hydroxy-2-deoxycytidine in Cockayne syndrome cells and its complementation by Escherichia coli formamidopyrimidine DNA glycosylase and endonuclease III
Free Radic. Biol. Med.
48
681-690
2010
Escherichia coli
brenda
Muftuoglu, M.; de Souza-Pinto, N.C.; Dogan, A.; Aamann, M.; Stevnsner, T.; Rybanska, I.; Kirkali, G.; Dizdaroglu, M.; Bohr, V.A.
Cockayne syndrome group B protein stimulates repair of formamidopyrimidines by NEIL1 DNA glycosylase
J. Biol. Chem.
284
9270-9279
2009
Homo sapiens, Mus musculus
brenda
Koval, V.V.; Kuznetsov, N.A.; Ishchenko, A.A.; Saparbaev, M.K.; Fedorova, O.S.
Real-time studies of conformational dynamics of the repair enzyme E. coli formamidopyrimidine-DNA glycosylase and its DNA complexes during catalytic cycle
Mutat. Res.
685
3-10
2010
Escherichia coli
brenda
Liu, M.; Bandaru, V.; Bond, J.P.; Jaruga, P.; Zhao, X.; Christov, P.P.; Burrows, C.J.; Rizzo, C.J.; Dizdaroglu, M.; Wallace, S.S.
The mouse ortholog of NEIL3 is a functional DNA glycosylase in vitro and in vivo
Proc. Natl. Acad. Sci. USA
107
4925-4930
2010
Mus musculus
brenda
Jaruga, P.; Xiao, Y.; Vartanian, V.; Lloyd, R.S.; Dizdaroglu, M.
Evidence for the involvement of DNA repair enzyme NEIL1 in nucleotide excision repair of (5'R)- and (5'S)-8,5'-cyclo-2'-deoxyadenosines
Biochemistry
49
1053-1055
2010
Mus musculus
brenda
Schalow, B.J.; Courcelle, C.T.; Courcelle, J.
Escherichia coli Fpg glycosylase is nonrendundant and required for the rapid global repair of oxidized purine and pyrimidine damage in vivo
J. Mol. Biol.
410
183-193
2011
Escherichia coli, Escherichia coli SR108
brenda
Le Bihan, Y.V.; Angeles Izquierdo, M.; Coste, F.; Aller, P.; Culard, F.; Gehrke, T.H.; Essalhi, K.; Carell, T.; Castaing, B.
5-Hydroxy-5-methylhydantoin DNA lesion, a molecular trap for DNA glycosylases
Nucleic Acids Res.
39
6277-6290
2011
Lactococcus lactis
brenda
Dunn, A.R.; Kad, N.M.; Nelson, S.R.; Warshaw, D.M.; Wallace, S.S.
Single Qdot-labeled glycosylase molecules use a wedge amino acid to probe for lesions while scanning along DNA
Nucleic Acids Res.
39
7487-7498
2011
Escherichia coli
brenda
Reddy, P.T.; Jaruga, P.; Nelson, B.C.; Lowenthal, M.; Dizdaroglu, M.
Stable isotope-labeling of DNA repair proteins, and their purification and characterization
Protein Expr. Purif.
78
94-101
2011
Escherichia coli
brenda
Rana, J.; Huang, H.
Actions of the Klenow fragment of DNA polymerase I and some DNA glycosylases on chemically stable analogues of N7-methyl-2-deoxyguanosine
Bioorg. Med. Chem.
21
6886-6892
2013
Escherichia coli
brenda
Duclos, S.; Aller, P.; Jaruga, P.; Dizdaroglu, M.; Wallace, S.S.; Doublie, S.
Structural and biochemical studies of a plant formamidopyrimidine-DNA glycosylase reveal why eukaryotic Fpg glycosylases do not excise 8-oxoguanine
DNA Repair
11
714-725
2012
Arabidopsis thaliana (O80358), Arabidopsis thaliana
brenda
Kain, J.; Karlsson, H.L.; Moeller, L.
DNA damage induced by micro- and nanoparticles - interaction with FPG influences the detection of DNA oxidation in the comet assay
Mutagenesis
27
491-500
2012
Escherichia coli
brenda
Kuznetsov, N.A.; Vorobjev, Y.N.; Krasnoperov, L.N.; Fedorova, O.S.
Thermodynamics of the multi-stage DNA lesion recognition and repair by formamidopyrimidine-DNA glycosylase using pyrrolocytosine fluorescence--stopped-flow pre-steady-state kinetics
Nucleic Acids Res.
40
7384-7392
2012
Escherichia coli
brenda
Foresta, M.; Izzotti, A.; La Maestra, S.; Micale, R.; Poggi, A.; Vecchio, D.; Frosina, G.
Accelerated repair and reduced mutagenicity of DNA damage induced by cigarette smoke in human bronchial cells transfected with E.coli formamidopyrimidine DNA glycosylase
PLoS ONE
9
e87984
2014
Escherichia coli
brenda
Prakash, A.; Doublie, S.; Wallace, S.S.
The Fpg/Nei family of DNA glycosylases: substrates, structures, and search for damage
Prog. Mol. Biol. Transl. Sci.
110
71-91
2012
Thermus thermophilus (O50606), Escherichia coli (P05523), Lactococcus lactis (P42371), Geobacillus stearothermophilus (P84131), Thermus thermophilus DSM 579 (O50606)
brenda
Ondovcik, S.L.; Preston, T.J.; McCallum, G.P.; Wells, P.G.
Expression of human oxoguanine glycosylase 1 or formamidopyrimidine glycosylase in human embryonic kidney 293 cells exacerbates methylmercury toxicity in vitro
Toxicol. Appl. Pharmacol.
271
41-48
2013
Homo sapiens
brenda
Song, J.; Yin, F.; Li, X.; Dong, N.; Zhu, Y.; Shao, Y.; Chen, B.; Jiang, W.; Li, C.Z.
Sensitive detection of formamidopyrimidine-DNA glycosylase activity based on target-induced self-primed rolling circle amplification and magnetic nanoprobes
Analyst
143
1593-1598
2018
Homo sapiens
brenda
Le Meur, R.; Culard, F.; Nadan, V.; Goffinont, S.; Coste, F.; Guerin, M.; Loth, K.; Landon, C.; Castaing, B.
The nucleoid-associated protein HU enhances 8-oxoguanine base excision by the formamidopyrimidine-DNA glycosylase
Biochem. J.
471
13-23
2015
Escherichia coli (P05523)
brenda
Sowlati-Hashjin, S.; Wetmore, S.D.
Structural insight into the discrimination between 8-oxoguanine glycosidic conformers by DNA repair rnzymes A molecular dynamics study of human oxoguanine glycosylase 1 and formamidopyrimidine-DNA glycosylase
Biochemistry
57
1144-1154
2018
Geobacillus stearothermophilus (P84131)
brenda
Popov, A.V.; Endutkin, A.V.; Vorobjev, Y.N.; Zharkov, D.O.
Molecular dynamics simulation of the opposite-base preference and interactions in the active site of formamidopyrimidine-DNA glycosylase
BMC Struct. Biol.
17
005
2017
Lactococcus lactis (P42371), Lactococcus lactis
brenda
Kiruba, G.S.; Xu, J.; Zelikson, V.; Lee, J.K.
Gas-phase studies of formamidopyrimidine glycosylase (Fpg) substrates
Chemistry
22
3881-3890
2016
Escherichia coli
brenda
Endutkin, A.V.; Koptelov, S.S.; Popov, A.V.; Torgasheva, N.A.; Lomzov, A.A.; Tsygankova, A.R.; Skiba, T.V.; Afonnikov, D.A.; Zharkov, D.O.
Residue coevolution reveals functionally important intramolecular interactions in formamidopyrimidine-DNA glycosylase
DNA Repair
69
24-33
2018
Escherichia coli (P05523), Escherichia coli
brenda
Minko, I.G.; Christov, P.P.; Li, L.; Stone, M.P.; McCullough, A.K.; Lloyd, R.S.
Processing of N5-substituted formamidopyrimidine DNA adducts by DNA glycosylases NEIL1 and NEIL3
DNA Repair
73
49-54
2019
Homo sapiens (Q96FI4), Homo sapiens, Mus musculus (Q8K203), Mus musculus
brenda
Arantes, L.S.; Nova, L.G.; Resende, B.C.; Bitar, M.; Coelho, I.E.; Miyoshi, A.; Azevedo, V.A.; Lara Dos Santos, L.; Machado, C.R.; de Oliveira Lopes, D.
The Corynebacterium pseudotuberculosis genome contains two formamidopyrimidine-DNA glycosylase enzymes, only one of which recognizes and excises 8-oxoguanine lesion
Gene
575
233-243
2016
Corynebacterium pseudotuberculosis (D9QB78), Corynebacterium pseudotuberculosis, Corynebacterium pseudotuberculosis 231 (D9QB78)
brenda
Li, H.; Endutkin, A.V.; Bergonzo, C.; Campbell, A.J.; de los Santos, C.; Grollman, A.; Zharkov, D.O.; Simmerling, C.
A dynamic checkpoint in oxidative lesion discrimination by formamidopyrimidine-DNA glycosylase
Nucleic Acids Res.
44
683-694
2016
Escherichia coli (P05523), Geobacillus stearothermophilus (P84131)
brenda