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3.2.2.23: DNA-formamidopyrimidine glycosylase

This is an abbreviated version!
For detailed information about DNA-formamidopyrimidine glycosylase, go to the full flat file.

Word Map on EC 3.2.2.23

Reaction

DNA containing ring-opened N7-methylguanine
+
H2O
=
deguanylated DNA
 +
2,6-diamino-4-hydroxy-5-(N-methyl)formamidopyrimidine

Synonyms

2,6-diamino-4-hydroxy-5(N-methyl)formamidopyrimidine-DNA glycosylase, 2,6-diamino-4-hydroxy-5N-formamidopyrimidine-DNA glycosylase, 2,6-diamino-4-hydroxy-5N-methyl-formamidopyrimidine-DNA glycosylase, 8-hydroxyguanine endonuclease, 8-oxoguanine DNA glycosylase, 8-oxoguanine-DNA glycosylase, deoxyribonucleate glycosidase, DNA glycohydrolase (releasing 2,6-diamino-4-hydroxy-5-(N-methyl)-formamidopyrimidine), DNA glycosylase, endonuclease VIII-like DNA glycosylase, Fapy DNA glycosylase, Fapy-DNA glycosylase, formamidopyrimidineDNA glycosylase, formamidopyrimidine DNA glycosylase, formamidopyrimidine glycosylase, formamidopyrimidine-DNA glycosyl hydrolase, formamidopyrimidine-DNA glycosylase, formamidopyrimidine-N-glycosylase, FPG, Fpg protein, FPG-1, FPG-2, Fpg-L, glycosidase, deoxyribonucleate formamidopyrimidine, More, Mtb-Fpg1, MutM, MutM1, NEH1, NEH2, Nei, NEIL1, NEIL3, OGG1

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.2 Hydrolysing N-glycosyl compounds
                3.2.2.23 DNA-formamidopyrimidine glycosylase

Cloned

Cloned on EC 3.2.2.23 - DNA-formamidopyrimidine glycosylase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21(DE3) cells
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expressed in Escherichia coli Rosetta (DE3) pLysS cells
expressed in Escherichia coli strain BH540
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expressed in HEK-293 cells
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expressed NCI-H727 non small cell lung carcinoma cells
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expression in Escherichia coli
expression in human bladder cell
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expression of C-terminally His-tagged wild-type NEIL3 in Escherichia coli, and expression of the C-terminally His-tagged glycosylase domain of Neil3, MmuNeil3DELTA324, in an Escherichia coli triple mutant lacking Fpg, Nei, and MutY glycosylase activities, the recombinant MmuNeil3 greatly reduced both the spontaneous mutation frequency and the level of FapyG in the DNA
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expression of EGFP-tagged enzyme in HEK-293T cells. Transformation of primary human fibroblasts of a Cockayne syndrome patient using SV40, expression of FPG in 5-hydroxy-2'-deoxycytidine repair defective human cells complements the disorder and leads to stable correction of the delayed removal of both oxidized purines and oxidized pyrimidines in the cells, detailed overview
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expression of K155A mutant and wild-type Fpg in Escherichia coli BL21(DE3) and BLR(DE3)
Fpg gene is cloned, encodes a 273 amino acids protein
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fpg structural gene is cloned and sequenced, overexpression in Escherichia coli HB101
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fpg-L gene is cloned, sequenced and overexpressed in Escherichia coli BH410, expression in Escherichia coli fpg and mutY mutants suppresses their spontaneous GC-TA mutator phenotype
gene fpg, DNA and amino acid sequence determination and analysis, sequence comparison and phylogenetic analysis
gene fpg, expression in Escherichia coli, expression increases the frequency of spontaneous reversions in the presence of plant FPG variant, quantitative overview
gene Rv2924c encoding Mtb-Fpg1, DNA and amino acid sequence determination and analysis, relationship between tandem repeat length and Mtb-fpg1 expression levels, quantitative expression analysis, overview
genotyping, overview. Expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
isolation of 2 cDNA clones: alternative splicing products AtFPG-1 and -2 of a single gene with different C-terminal sequences, amino acid sequences, expression of both proteins in Escherichia coli BL21(DE3)
NEH1 is cloned, sequenced and expressed in Escherichia coli BL21(DE3), chromosomal location: 15q25
NEH2, chromosomal location: 4q35
P2E, P2T and P2G mutant Fpg are cloned and expressed in Escherichia coli BH20(fpg-)
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