3.2.2.23: DNA-formamidopyrimidine glycosylase
This is an abbreviated version!
For detailed information about DNA-formamidopyrimidine glycosylase, go to the full flat file.
Word Map on EC 3.2.2.23
-
3.2.2.23
-
strand
-
comet
-
glycosylases
-
genotoxic
-
purine
-
single-stranded
-
abasic
-
duplex
-
fpg-sensitive
-
thymine
-
excises
-
7,8-dihydro-8-oxoguanine
-
ape1
-
ring-opened
-
8-oxo-7,8-dihydroguanine
-
8-oxodg
-
endonucleases
-
8-oxo-7,8-dihydro-2'-deoxyguanosine
-
5-hydroxyuracil
-
micronucleus
-
transversions
-
neils
-
alkali-labile
-
biomonitoring
-
3-methyladenine-dna
-
mispairs
-
interstrand
-
lesion-specific
-
spiroiminodihydantoin
-
bromate
-
medicine
-
2'-deoxyguanosine
-
klenow
-
ap-endonuclease
-
uvrabc
-
miscoding
-
base-excision
-
apurinic
-
8-oxo-2'-deoxyguanosine
-
monoadducts
-
dihydrouracil
-
enzyme-modified
-
unhook
-
mutyh
-
endoiii
-
analysis
- 3.2.2.23
- strand
- comet
- glycosylases
-
genotoxic
- purine
-
single-stranded
-
abasic
- duplex
-
fpg-sensitive
- thymine
-
excises
- 7,8-dihydro-8-oxoguanine
- ape1
-
ring-opened
- 8-oxo-7,8-dihydroguanine
-
8-oxodg
- endonucleases
-
8-oxo-7,8-dihydro-2'-deoxyguanosine
- 5-hydroxyuracil
-
micronucleus
-
transversions
-
neils
-
alkali-labile
-
biomonitoring
-
3-methyladenine-dna
- mispairs
-
interstrand
-
lesion-specific
- spiroiminodihydantoin
- bromate
- medicine
- 2'-deoxyguanosine
-
klenow
- ap-endonuclease
-
uvrabc
-
miscoding
-
base-excision
-
apurinic
- 8-oxo-2'-deoxyguanosine
-
monoadducts
- dihydrouracil
-
enzyme-modified
-
unhook
-
mutyh
-
endoiii
- analysis
Reaction
Synonyms
2,6-diamino-4-hydroxy-5(N-methyl)formamidopyrimidine-DNA glycosylase, 2,6-diamino-4-hydroxy-5N-formamidopyrimidine-DNA glycosylase, 2,6-diamino-4-hydroxy-5N-methyl-formamidopyrimidine-DNA glycosylase, 8-hydroxyguanine endonuclease, 8-oxoguanine DNA glycosylase, 8-oxoguanine-DNA glycosylase, deoxyribonucleate glycosidase, DNA glycohydrolase (releasing 2,6-diamino-4-hydroxy-5-(N-methyl)-formamidopyrimidine), DNA glycosylase, endonuclease VIII-like DNA glycosylase, Fapy DNA glycosylase, Fapy-DNA glycosylase, formamidopyrimidineDNA glycosylase, formamidopyrimidine DNA glycosylase, formamidopyrimidine glycosylase, formamidopyrimidine-DNA glycosyl hydrolase, formamidopyrimidine-DNA glycosylase, formamidopyrimidine-N-glycosylase, FPG, Fpg protein, FPG-1, FPG-2, Fpg-L, glycosidase, deoxyribonucleate formamidopyrimidine, More, Mtb-Fpg1, MutM, MutM1, NEH1, NEH2, Nei, NEIL1, NEIL3, OGG1
ECTree
3.2.2.23 DNA-formamidopyrimidine glycosylaseAdvanced search results
results (
results (Substrates Products
Substrates Products on EC 3.2.2.23 - DNA-formamidopyrimidine glycosylase
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SUBSTRATE 
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
2,6-diamino-4-hydroxy-5-formamidopyrimidine-DNA + H2O
2,6-diamino-4-hydroxy-5-formamidopyrimidine + DNA
2,6-diamino-4-hydroxy-5-formamidopyrimidine:Cyt oligodeoxynucleotide + H2O
2,6-diamino-4-hydroxy-5-formamidopyrimidine + Cyt oligodeoxynucleotide
2,6-diamino-4-hydroxy-5-formamidopyrimidine:Cyt-DNA + H2O
2,6-diamino-4-hydroxy-5-formamidopyrimidine + Cyt-DNA
-
-
-
?
23mer oligonucleotide duplex containing 8-oxoguanine + H2O
8-oxoguanine + oligonucleotide
-
-
-
?
53mer containing 8-oxoguanine + H2O
53mer with an abasic site + 8-oxoguanine
-
-
-
?
8-oxo-7,8-dihydroguanine:Cyt oligodeoxynucleotide + H2O
8-oxo-7,8-dihydroguanine + Cyt oligodeoxynucleotide
8-oxo-7,8-dihydroguanine:Cyt-DNA + H2O
8-oxo-7,8-dihydroguanine + Cyt-DNA
-
-
-
?
depurinated supercoiled plasmid DNA + H2O
?
-
the degree of supercoiling of assay plasmid DNA does not affect enzyme activity
-
-
?
DNA containing 2'-deoxy-8-oxonebularine residues + H2O
?
23mer oligonucleotide containing a single site, very poor substrate
-
-
?
DNA containing 2,6-diamino-4-hydroxy-5-formamidopyrimidine residues + H2O
2,6-diamino-4-hydroxy-5-formamidopyrimidine + DNA
DNA containing 2,6-diamino-4-hydroxy-5-formamidopyrimidine residues + H2O
DNA + 2,6-diamino-4-hydroxy-5-formamidopyrimidine
DNA containing 2,6-diamino-4-hydroxyformamidopyrimidine residues + H2O
2,6-diamino-4-hydroxyformamidopyrimidine + DNA
-
repair of the major DNA lesions 7,8-dihydro-8-oxoguanine and 2,6-diamino-4-hydroxyformamidopyrimidine formed by reactive oxidative species
-
-
?
DNA containing 4,6-diamino-5-formamidopyrimidine residues + H2O
4,6-diamino-5-formamidopyrimidine + DNA
DNA containing 4,6-diamino-5-formamidopyrimidine residues + H2O
DNA + 4,6-diamino-5-formamidopyrimidine
DNA containing 5,6-dihydrothymine residues + H2O
?
-
dublex 33mer oligonucleotide, poor substrate
-
-
?
DNA containing 5-hydroxy-5-methylhydantoin residues opposite cytosine + H2O
5-hydroxy-5-methylhydantoin + DNA
-
excellent substrate when the lesion is opposite a cytosine, poor substrate when the lesion is opposite a adenine
-
-
?
DNA containing 7,8-dihydro-8-oxoguanine residues + H2O
DNA + 7,8-dihydro-8-oxoguanine
-
-
-
-
?
DNA containing 7-methyl-7-deazaguanine residues + H2O
7-methyl-7-deazaguanine + DNA
-
-
-
-
?
DNA containing 7-methyl-8-oxo-2'-deoxyguanosine + H2O
7-methyl-8-oxo-2'-deoxyguanosine + DNA
DNA containing 8-hydroxyadenine residues + H2O
8-hydroxyadenine + DNA
-
poor substrate
-
-
?
DNA containing 8-oxo-2'-deoxyguanosine residues + H2O
DNA + 8-oxo-2'-deoxyguanosine
-
-
-
-
?
DNA containing 8-oxo-7,8-dihydroguanine residues + H2O
DNA + 8-hydroxyguanine
-
-
-
-
?
DNA containing 8-oxo-7,8-dihydroguanine residues + H2O
DNA + 8-oxo-7,8-dihydroguanine
-
-
-
?
DNA containing 8-oxo-7,8-dihydropurine + H2O
8-oxo-7,8-dihydropurine + DNA
-
poor substrate when the lesion is opposite a cytosine
-
-
?
DNA containing 8-oxo-guanine residues mispaired to adenine + H2O
DNA + 8-oxoguanine
-
-
-
-
?
DNA containing 8-oxo-guanine residues mispaired to guanine + H2O
DNA + 8-oxoguanine
-
-
-
-
?
DNA containing 8-oxo-guanine residues mispaired to thymine + H2O
DNA + 8-oxoguanine
-
-
-
-
?
DNA containing 8-oxoguanine opposite A + H2O
8-oxoguanine + DNA
poor substrate, analysis of rate constants of conformational transitions
-
-
?
DNA containing 8-oxoguanine opposite C + H2O
8-oxoguanine + DNA
good substrate, analysis of rate constants of conformational transitions
-
-
?
DNA containing 8-oxoguanine opposite G + H2O
8-oxoguanine + DNA
analysis of rate constants of conformational transitions
-
-
?
DNA containing 8-oxoguanine opposite T + H2O
8-oxoguanine + DNA
analysis of rate constants of conformational transitions
-
-
?
DNA containing dihydrouracil residues + H2O
?
active as a DNA glycosylase/AP lyase with dihydrouracil 31mer dublex oligo substrate, similar activity with A, C or G opposite the lesion, reduced with T opposite
-
-
?
DNA containing formamidopyrimidine-guanine residues + H2O
4,6-diamino-5-formamidopyrimidine + DNA
DNA containing guanidinohydantoin + H2O
guanidinohydantoin + DNA
-
for DNA duplex length of 30 bp, the excision efficiency in pairs with C, G, or T is similar to 8-oxoguanine. Opposite A, the base removal activity is more efficient than removal of 8-oxoguanine
-
-
?
DNA containing methylated formamidopyrimidine-guanine residues + H2O
2,6-diamino-4-hydroxy-5-(N-methyl)formamidopyrimidine + DNA
DNA containing oxoG opposite C or A + H2O
2,6-diamino-4-hydroxy-5-formamidopyrimidine + DNA
-
-
-
?
DNA containing ring-opened N7-methylguanine + H2O
2,6-diamino-4-hydroxy-5-(N-methyl)formamidopyrimidine + DNA
-
repairs oxidative DNA damage by efficiently removing formamidopyrimidine lesions and 8-oxoguanine residues from DNA
-
-
?
DNA containing ring-opened N7-methylguanine residues + H2O
2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine + DNA
DNA containing spiroiminodihydantoin + H2O
spiroiminodihydantoin + DNA
-
for DNA duplex length of 30 bp, the excision efficiency in pairs with C, G, or T is similar to 8-oxoguanine. Opposite A, the base removal activity is more efficient than removal of 8-oxoguanine
-
-
?
ds oligodeoxynucleotides containing N5-alkyl formamidopyrimidine + H2O
2,6-diamino-4-hydroxy-5-formamidopyrimidine + ds oligonucleotide
-
-
-
?
oligonucleotide containing 8-oxo-2'-deoxyguanosine residue + H2O
oligonucleotide + 8-oxo-2'-deoxyguanosine
-
-
-
-
?
oligonucleotide containing abasic site residue + H2O
oligonucleotide + abasic site
-
-
-
-
?
oligonucleotide containing tetrahydrofuran residue + H2O
oligonucleotide + tetrahydrofuran
-
-
-
-
?
ss oligodeoxynucleotides containing N5-alkyl formamidopyrimidine + H2O
2,6-diamino-4-hydroxy-5-formamidopyrimidine + ds oligonucleotide
DNA base alkylation damage induced by nitrogen mustard
-
-
?
13mer oligonucleotide duplex containing 8-oxoguanine + H2O
8-oxoguanine + oligonucleotide
-
-
-
-
?
13mer oligonucleotide duplex containing 8-oxoguanine + H2O
8-oxoguanine + oligonucleotide
-
-
-
?
2,6-diamino-4-hydroxy-5-formamidopyrimidine-DNA + H2O
2,6-diamino-4-hydroxy-5-formamidopyrimidine + DNA
-
-
-
-
?
2,6-diamino-4-hydroxy-5-formamidopyrimidine-DNA + H2O
2,6-diamino-4-hydroxy-5-formamidopyrimidine + DNA
-
-
-
-
?
2,6-diamino-4-hydroxy-5-formamidopyrimidine:Cyt oligodeoxynucleotide + H2O
2,6-diamino-4-hydroxy-5-formamidopyrimidine + Cyt oligodeoxynucleotide
-
-
-
-
?
2,6-diamino-4-hydroxy-5-formamidopyrimidine:Cyt oligodeoxynucleotide + H2O
2,6-diamino-4-hydroxy-5-formamidopyrimidine + Cyt oligodeoxynucleotide
-
-
-
?
5,6-dihydrouracil-DNA + H2O
5,6-dihydrouracil + DNA
-
DHU is excised from DNA by a number of DNA glycosylases including Fpg and Nei
-
-
?
5,6-dihydrouracil-DNA + H2O
5,6-dihydrouracil + DNA
-
DHU is formed in DNA from cytosine under the action of OH radicals under ionizing radiation
-
-
?
60mer containing 8-oxoguanine + H2O
60mer with an abasic site + 8-oxoguanine
-
-
-
?
60mer containing 8-oxoguanine + H2O
60mer with an abasic site + 8-oxoguanine
-
-
-
?
8-oxo-7,8-dihydroguanine:Cyt oligodeoxynucleotide + H2O
8-oxo-7,8-dihydroguanine + Cyt oligodeoxynucleotide
-
-
-
-
?
8-oxo-7,8-dihydroguanine:Cyt oligodeoxynucleotide + H2O
8-oxo-7,8-dihydroguanine + Cyt oligodeoxynucleotide
-
-
-
?
DNA + H2O
?
both AtFPG-1 and -2 cleave DNA containing apurinic sites and UV-irradiated and oxidized DNA
-
-
?
DNA + H2O
?
catalyzes the initial steps in the repair of DNA containing oxidized purines
-
-
?
DNA + H2O
?
-
enzyme has N-glycosylase and apurinic/apyrimidinic lyase activity
-
-
?
DNA + H2O
?
-
with apurinic/apyrimidinic lyase activity, catalyzes beta and delta elimination reactions
-
-
?
DNA + H2O
?
with apurinic/apyrimidinic lyase activity, catalyzes beta and delta elimination reactions
-
-
?
DNA + H2O
?
-
role of Lys-155 for substrate binding and product release, AP lyase mechanism
-
-
?
DNA + H2O
?
-
FpG-DNA interactions establish contacts with DNA ligands, which span no more than 9 base-pairs, structural studies of Fpg-DNA complexes
-
-
?
DNA + H2O
?
-
excises oxidized purines from damaged DNA, Schiff base intermediate, enzyme structure, bilobal protein with a wide, positive charged DNA-binding groove and a helix-2-turn-helix motif that participates in DNA binding, damage recognition, catalytic mechanism
-
-
?
DNA + H2O
?
-
removes oxidized purines from oxidatively damaged DNA
-
-
?
DNA + H2O
?
removes oxidized purines from oxidatively damaged DNA
-
-
?
DNA + H2O
?
-
catalytic mechanism involves formation of Schiff base intermediate between DNA containing an oxidized residue and the N-terminal Pro-2 of Fpg, mendatory role of P-2 in 7,8-dihydro-8-oxoguanine-DNA glycosylase and AP lyase activity, but less in 2,6-diamino-4-hydroxy-5-N-methyl-formamidopyrimidine-DNA glycosylase activity
-
-
?
DNA + H2O
?
-
active site is located within the first 73 amino acids of the N-terminus
-
-
?
DNA + H2O
?
-
excises purine bases with ring-opened imidazoles, associated activity that nicks DNA at apurinic/apyrimidinic sites, mechanism of cleavage involves beta elimination
-
-
?
DNA + H2O
?
-
enzyme has N-glycosylase and apurinic/apyrimidinic lyase activity
-
-
?
DNA + H2O
?
enzyme has N-glycosylase and apurinic/apyrimidinic lyase activity
-
-
?
DNA + H2O
?
-
enzyme catalyzes the nicking of both the phosphodiester bonds 3' and 5' of apurinic or apyrimidinic sites in DNA so that the base-free deoxyribose is replaced by a gap limited by 3'-phosphate and 5'-phosphate ends, the 2 nickings are not the result of hydrolytic processes, the enzyme rather catalyzes a beta-elimination reaction immediately followed by a delta-elimination
-
-
?
DNA + H2O
?
-
DNA repair enzyme specific for the removal of purine-derived lesions from DNA damaged by free radicals and other oxidative processes
-
-
?
DNA + H2O
?
-
plays an important role in base excision repair of oxidatively damaged DNA
-
-
?
DNA + H2O
?
-
involved in the repair of oxidized purines generated in the genome by endogenous or exogenous oxidative stress
-
-
?
DNA + H2O
?
-
DNA base excision repair enzyme
-
-
?
DNA + H2O
?
-
bifunctional base excision repair enzyme: DNA glycosylase/AP lyase
-
-
?
DNA + H2O
?
-
with apurinic/apyrimidinic lyase activity, catalyzes beta and delta elimination reactions
-
-
?
DNA + H2O
?
-
FpG-DNA interactions establish contacts with DNA ligands, which span no more than 9 base-pairs, structural studies of Fpg-DNA complexes
-
-
?
DNA + H2O
?
-
enzyme has N-glycosylase and apurinic/apyrimidinic lyase activity
-
-
?
DNA + H2O
?
-
bifunctional base excision repair enzyme: DNA glycosylase/AP lyase
-
-
?
DNA + H2O
?
-
removes oxidized purines from oxidatively damaged DNA
-
-
?
DNA + H2O
?
-
enzyme has N-glycosylase and apurinic/apyrimidinic lyase activity
-
-
?
DNA + H2O
?
-
involved in the repair of oxidized purines generated in the genome by endogenous or exogenous oxidative stress
-
-
?
DNA + H2O
?
-
removes oxidized purines from oxidatively damaged DNA
-
-
?
DNA + H2O
?
-
enzyme has N-glycosylase and apurinic/apyrimidinic lyase activity
-
-
?
DNA + H2O
?
-
enzyme has N-glycosylase and apurinic/apyrimidinic lyase activity
-
-
?
DNA + H2O
?
with apurinic/apyrimidinic lyase activity, catalyzes beta and delta elimination reactions
-
-
?
DNA + H2O
?
excises formamidopyrimidines from damaged DNA and oxidized pyrimidines and 8-oxoguanine from oligodeoxynucleotides, role of the N-terminal Pro as its active site
-
-
?
DNA + H2O
?
enzyme has N-glycosylase and apurinic/apyrimidinic lyase activity
-
-
?
DNA + H2O
?
involved in replication-associated repair of oxidized bases
-
-
?
DNA + H2O
?
-
removes a wide range of oxidized purines, such as 8-oxoguanine and imidazole ring-opened purines, from oxidatively damaged DNA, DNA glycosylase/AP lyase activity, substrate recognition
-
-
?
DNA + H2O
?
-
enzyme has N-glycosylase and apurinic/apyrimidinic lyase activity
-
-
?
DNA + H2O
?
enzyme has N-glycosylase and apurinic/apyrimidinic lyase activity
-
-
?
DNA + H2O
?
important role in protecting DNA against oxidative free radicals and reactive oxygen-derived species
-
-
?
DNA + H2O
?
-
bifunctional base excision repair enzyme: DNA glycosylase/AP lyase
-
-
?
DNA containing 2,6-diamino-4-hydroxy-5-formamidopyrimidine residues + H2O
2,6-diamino-4-hydroxy-5-formamidopyrimidine + DNA
-
similar specificity as FapyAde-DNA, better substrate than 8-hydroxyguanine-DNA
-
-
?
DNA containing 2,6-diamino-4-hydroxy-5-formamidopyrimidine residues + H2O
2,6-diamino-4-hydroxy-5-formamidopyrimidine + DNA
-
FapyGua
-
-
?
DNA containing 2,6-diamino-4-hydroxy-5-formamidopyrimidine residues + H2O
2,6-diamino-4-hydroxy-5-formamidopyrimidine + DNA
-
-
-
-
?
DNA containing 2,6-diamino-4-hydroxy-5-formamidopyrimidine residues + H2O
2,6-diamino-4-hydroxy-5-formamidopyrimidine + DNA
-
removal with similar specificity as 4,6-diamino-5-formamidopyrimidine and 8-hydroxyguanine
-
?
DNA containing 2,6-diamino-4-hydroxy-5-formamidopyrimidine residues + H2O
2,6-diamino-4-hydroxy-5-formamidopyrimidine + DNA
-
FapyGua
-
?
DNA containing 2,6-diamino-4-hydroxy-5-formamidopyrimidine residues + H2O
2,6-diamino-4-hydroxy-5-formamidopyrimidine + DNA
-
FapyGua
-
-
?
DNA containing 2,6-diamino-4-hydroxy-5-formamidopyrimidine residues + H2O
2,6-diamino-4-hydroxy-5-formamidopyrimidine + DNA
FapyGua
-
-
?
DNA containing 2,6-diamino-4-hydroxy-5-formamidopyrimidine residues + H2O
2,6-diamino-4-hydroxy-5-formamidopyrimidine + DNA
FapyGua
-
-
?
DNA containing 2,6-diamino-4-hydroxy-5-formamidopyrimidine residues + H2O
2,6-diamino-4-hydroxy-5-formamidopyrimidine + DNA
-
-
-
-
?
DNA containing 2,6-diamino-4-hydroxy-5-formamidopyrimidine residues + H2O
DNA + 2,6-diamino-4-hydroxy-5-formamidopyrimidine
-
-
-
-
?
DNA containing 2,6-diamino-4-hydroxy-5-formamidopyrimidine residues + H2O
DNA + 2,6-diamino-4-hydroxy-5-formamidopyrimidine
-
-
-
?
DNA containing 2,6-diamino-4-hydroxy-5-formamidopyrimidine residues + H2O
DNA + 2,6-diamino-4-hydroxy-5-formamidopyrimidine
-
-
-
-
?
DNA containing 4,6-diamino-5-formamidopyrimidine residues + H2O
4,6-diamino-5-formamidopyrimidine + DNA
-
similar specificity as FapyGua-DNA, better substrate than 8-hydroxyguanine-DNA
-
-
?
DNA containing 4,6-diamino-5-formamidopyrimidine residues + H2O
4,6-diamino-5-formamidopyrimidine + DNA
-
FapyAde
-
-
?
DNA containing 4,6-diamino-5-formamidopyrimidine residues + H2O
4,6-diamino-5-formamidopyrimidine + DNA
-
-
-
-
?
DNA containing 4,6-diamino-5-formamidopyrimidine residues + H2O
4,6-diamino-5-formamidopyrimidine + DNA
-
removal with similar specificity as 2,6-diamino-4-hydroxy-5-formamidopyrimidine and 8-hydroxyguanine
-
?
DNA containing 4,6-diamino-5-formamidopyrimidine residues + H2O
4,6-diamino-5-formamidopyrimidine + DNA
-
FapyAde
-
?
DNA containing 4,6-diamino-5-formamidopyrimidine residues + H2O
4,6-diamino-5-formamidopyrimidine + DNA
-
FapyAde
-
-
?
DNA containing 4,6-diamino-5-formamidopyrimidine residues + H2O
4,6-diamino-5-formamidopyrimidine + DNA
FapyAde
-
-
?
DNA containing 4,6-diamino-5-formamidopyrimidine residues + H2O
4,6-diamino-5-formamidopyrimidine + DNA
FapyAde
-
-
?
DNA containing 4,6-diamino-5-formamidopyrimidine residues + H2O
4,6-diamino-5-formamidopyrimidine + DNA
-
-
-
-
?
DNA containing 4,6-diamino-5-formamidopyrimidine residues + H2O
DNA + 4,6-diamino-5-formamidopyrimidine
-
-
-
-
?
DNA containing 4,6-diamino-5-formamidopyrimidine residues + H2O
DNA + 4,6-diamino-5-formamidopyrimidine
-
-
-
?
DNA containing 4,6-diamino-5-formamidopyrimidine residues + H2O
DNA + 4,6-diamino-5-formamidopyrimidine
-
-
-
-
?
DNA containing 5-hydroxy-2'-deoxyuridine + H2O
?
-
double-stranded oligonucleotides, N-glycosylase/beta,delta-elimination reaction
-
-
?
DNA containing 5-hydroxy-2'-deoxyuridine + H2O
?
dublex oligodeoxynucleotide containing 5-hydroxyuracil when paired with G
-
-
?
DNA containing 5-hydroxycytosine residues + H2O
5-hydroxycytosine + DNA
-
-
-
-
?
DNA containing 5-hydroxycytosine residues + H2O
5-hydroxycytosine + DNA
-
-
-
-
?
DNA containing 5-hydroxycytosine residues + H2O
?
-
double-stranded oligonucleotides containing 5-hydroxy-2'-deoxycytidine, N-glycosylase/beta,delta-elimination reaction
-
-
?
DNA containing 5-hydroxycytosine residues + H2O
?
-
dublex 33mer oligonucleotide, excision mechanism
-
-
?
DNA containing 5-hydroxyuracil residues + H2O
5-hydroxyuracil + DNA
-
-
-
-
?
DNA containing 5-hydroxyuracil residues + H2O
5-hydroxyuracil + DNA
-
-
-
-
?
DNA containing 7-deaza-2'-deoxyguanosine + H2O
7-deaza-2'-deoxyguanosine + DNA
-
-
-
-
?
DNA containing 7-deaza-2'-deoxyguanosine + H2O
7-deaza-2'-deoxyguanosine + DNA
-
-
-
-
?
DNA containing 7-hydro-8-oxoguanine + H2O
7-hydro-8-oxoguanine + DNA
-
repairs oxidative DNA damage by efficiently removing formamidopyrimidine lesions and 8-oxoguanine residues from DNA
-
-
?
DNA containing 7-hydro-8-oxoguanine + H2O
7-hydro-8-oxoguanine + DNA
-
dublex, primary physiological substrate, DNA repair
-
-
?
DNA containing 7-hydro-8-oxoguanine residues + H2O
DNA + 7-hydro-8-oxoguanine
-
-
-
-
?
DNA containing 7-hydro-8-oxoguanine residues + H2O
DNA + 7-hydro-8-oxoguanine
24-oligomer, Lys-155 directly interacts with the C8 oxygen of 8-oxopurines involving proton transfer or transient formation of an ion pair between enzyme and substrate, mechanism
-
?
DNA containing 7-hydro-8-oxoguanine residues + H2O
DNA + 7-hydro-8-oxoguanine
DNA glycosylase/AP lyase activity
-
?
DNA containing 7-hydro-8-oxoguanine residues + H2O
DNA + 7-hydro-8-oxoguanine
-
the C-8 keto group of 8-oxodeoxyguanine and the carbonyl moiety of formamidopyrimidine enable the enzyme to recognize and bind duplex DNA containing modified bases
-
-
?
DNA containing 7-hydro-8-oxoguanine residues + H2O
DNA + 7-hydro-8-oxoguanine
-
mechanism involving protonation at O-6 of 8-oxodeoxyguanine
-
-
?
DNA containing 7-hydro-8-oxoguanine residues + H2O
DNA + 7-hydro-8-oxoguanine
-
dublex 20-oligomer, catalytic mechanism with enzyme-substrate Schiff base intermediate, amino terminal localization of the catalytic site, C-8 keto group of 8-oxodeoxyguanine plays a critical role in binding enzyme
-
-
?
DNA containing 7-hydro-8-oxoguanine residues + H2O
DNA + 7-hydro-8-oxoguanine
-
kinetic mechanism, 3 activities: DNA-glycosylase, beta-elimination/AP-lyase and delta elimination, 12-nucleotide dublex containing 8-oxo-G in the sixth position of one strand, conformational transitions of Fpg protein during the catalytic process
-
-
?
DNA containing 7-hydro-8-oxoguanine residues + H2O
DNA + 7-hydro-8-oxoguanine
-
mechanism involving protonation at O-6 of 8-oxodeoxyguanine
-
-
?
DNA containing 7-hydro-8-oxoguanine residues + H2O
DNA + 7-hydro-8-oxoguanine
-
dublex 20-oligomer, catalytic mechanism with enzyme-substrate Schiff base intermediate, amino terminal localization of the catalytic site, C-8 keto group of 8-oxodeoxyguanine plays a critical role in binding enzyme
-
-
?
DNA containing 7-hydro-8-oxoguanine residues + H2O
DNA + 7-hydro-8-oxoguanine
excises 8-oxoguanine from 31mer oligodeoxynucleotide, most active with G, followed by T, opposite the lesion, weak activity with C or A opposite
-
-
?
DNA containing 7-hydro-8-oxoguanine residues + H2O
DNA + 7-hydro-8-oxoguanine
-
removes 8-oxoguanine from oxidatively damaged DNA
-
-
?
DNA containing 7-hydro-8-oxoguanine residues + H2O
DNA + 7-hydro-8-oxoguanine
-
DNA glycosylase/AP lyase activity
-
-
?
DNA containing 7-methyl-8-oxo-2'-deoxyguanosine + H2O
7-methyl-8-oxo-2'-deoxyguanosine + DNA
-
-
-
-
?
DNA containing 7-methyl-8-oxo-2'-deoxyguanosine + H2O
7-methyl-8-oxo-2'-deoxyguanosine + DNA
-
-
-
-
?
DNA containing 8-hydroxyguanine residues + H2O
8-hydroxyguanine + DNA
AtFPG-1, but not AtFPG-2, cleaves double-stranded oligonucleotides containing 8-oxoguanine
-
-
?
DNA containing 8-hydroxyguanine residues + H2O
8-hydroxyguanine + DNA
-
formamidopyrimidines are preferred over 8-hydroxyguanine
-
-
?
DNA containing 8-hydroxyguanine residues + H2O
8-hydroxyguanine + DNA
-
-
-
-
?
DNA containing 8-hydroxyguanine residues + H2O
8-hydroxyguanine + DNA
-
DNA glycosylase/AP lyase activity
-
-
?
DNA containing 8-hydroxyguanine residues + H2O
8-hydroxyguanine + DNA
-
34mer oligonucleotide containing a single 7,8-dihydro-8-oxoguanine residue
-
-
?
DNA containing 8-hydroxyguanine residues + H2O
8-hydroxyguanine + DNA
-
dublex oligodeoxynucleotides containing 8-oxo-7,8-dihydro-2-deoxyguanosine positioned opposite dC, dG or dT are cleaved, but not opposite dA or single-stranded DNA, cleaves 3 and 5 to the modified base
-
-
?
DNA containing 8-hydroxyguanine residues + H2O
8-hydroxyguanine + DNA
-
comparison of repair activities of human OGG1 and Escherichia coli Fpg, enzymes show distinct preferences for the base opposite 8-oxoguanine, mechanism via Schiff base intermediate
treatment of 7,8-dihydro-8-oxoguanine with Fpg results in delta-elimination products
?
DNA containing 8-hydroxyguanine residues + H2O
8-hydroxyguanine + DNA
-
mendatory role of P-2 in 7,8-dihydro-8-oxoguanine-DNA glycosylase activity
-
-
?
DNA containing 8-hydroxyguanine residues + H2O
8-hydroxyguanine + DNA
-
7,8-dihydro-8-oxo-2-deoxyguanosine, natural substrate, substrate recognition, mechanism
-
-
?
DNA containing 8-hydroxyguanine residues + H2O
8-hydroxyguanine + DNA
-
readily incises dublexes with cytosine, thymine or guanine opposite, but much slower with adenine opposite 7,8-dihydro-8-oxoguanine, 2 activities: DNA-glycosylase and DNA nicking at abasic sites
treatment of 7,8-dihydro-8-oxoguanine with Fpg results in delta-elimination products
?
DNA containing 8-hydroxyguanine residues + H2O
8-hydroxyguanine + DNA
-
7,8-dihydro-8-oxoguanine opposite C dublex DNA, formation of a Schiff base intermediate, important role for Lys-57 in the 7,8-dihydro-8-oxoG-DNA glycolase activity in vitro and in vivo
-
-
?
DNA containing 8-hydroxyguanine residues + H2O
8-hydroxyguanine + DNA
-
7,8-dihydro-8-oxoguanine-DNA
-
-
?
DNA containing 8-hydroxyguanine residues + H2O
8-hydroxyguanine + DNA
-
7,8-dihydro-8-oxoguanine-DNA
treatment of 7,8-dihydro-8-oxoguanine with Fpg results in delta-elimination products
?
DNA containing 8-hydroxyguanine residues + H2O
8-hydroxyguanine + DNA
-
removal with similar specificity as 4,6-diamino-5-formamidopyrimidine and 2,6-diamino-4-hydroxy-5-formamidopyrimidine, important role of Lys-57 in Fpg activity for 8-hydroxyguanine, but lesser significant role for formamidopyrimidines, Pro-2 is critical for substrate recognition and in catalysis of its excision
-
-
?
DNA containing 8-hydroxyguanine residues + H2O
8-hydroxyguanine + DNA
-
54 nt DNA oligomer, CoFpg and ZnFpg are equally active at cleaving the DNA at the site of the oxidized guanine
-
-
?
DNA containing 8-hydroxyguanine residues + H2O
8-hydroxyguanine + DNA
-
natural substrate: 7,8-dihydro-8-oxo-dG, DNA base excision repair
-
-
?
DNA containing 8-hydroxyguanine residues + H2O
8-hydroxyguanine + DNA
-
dublex, primary physiological substrate is 7,8-dihydro-8-oxoguanine-DNA, DNA repair
-
-
?
DNA containing 8-hydroxyguanine residues + H2O
8-hydroxyguanine + DNA
-
repair of the major DNA lesions 7,8-dihydro-8-oxoguanine and 2,6-diamino-4-hydroxyformamidopyrimidine formed by reactive oxidative species
-
-
?
DNA containing 8-hydroxyguanine residues + H2O
8-hydroxyguanine + DNA
-
-
-
-
?
DNA containing 8-hydroxyguanine residues + H2O
8-hydroxyguanine + DNA
-
DNA glycosylase/AP lyase activity
-
-
?
DNA containing 8-hydroxyguanine residues + H2O
8-hydroxyguanine + DNA
-
34mer oligonucleotide containing a single 7,8-dihydro-8-oxoguanine residue
-
-
?
DNA containing 8-hydroxyguanine residues + H2O
8-hydroxyguanine + DNA
34mer oligonucleotide containing a single 7,8-dihydro-8-oxoguanine residue
-
-
?
DNA containing 8-hydroxyguanine residues + H2O
8-hydroxyguanine + DNA
readily incises dublexes with cytosine, thymine or guanine opposite, but much slower with adenine opposite 7,8-dihydro-8-oxoguanine, 2 activities: DNA-glycosylase and DNA nicking at abasic sites
-
-
?
DNA containing 8-oxo-2'-deoxyguanosine + H2O
8-oxo-2'-deoxyguanosine + DNA
-
-
-
-
?
DNA containing 8-oxo-2'-deoxyguanosine + H2O
8-oxo-2'-deoxyguanosine + DNA
-
-
-
-
?
DNA containing 8-oxoguanine residues + H2O
8-oxoguanine + DNA
-
8-oxoguanine residues opposite cytosine
-
-
?
DNA containing 8-oxoguanine residues + H2O
8-oxoguanine + DNA
-
-
-
-
?
DNA containing 8-oxoguanine residues + H2O
DNA + 8-oxoguanine
best substrate
-
-
?
DNA containing 8-oxoguanine residues + H2O
DNA + 8-oxoguanine
best substrate
-
-
?
DNA containing 8-oxoguanine residues + H2O
DNA + 8-oxoguanine
best substrate
-
-
?
DNA containing 8-oxoguanine residues + H2O
DNA + 8-oxoguanine
best substrate
-
-
?
DNA containing 8-oxoguanine residues + H2O
DNA + 8-oxoguanine
best substrate
-
-
?
DNA containing 8-thio-2'-deoxyguanosine + H2O
8-thio-2'-deoxyguanosine + DNA
-
-
-
-
?
DNA containing 8-thio-2'-deoxyguanosine + H2O
8-thio-2'-deoxyguanosine + DNA
-
-
-
-
?
DNA containing formamidopyrimidine-guanine residues + H2O
4,6-diamino-5-formamidopyrimidine + DNA
-
-
-
?
DNA containing formamidopyrimidine-guanine residues + H2O
4,6-diamino-5-formamidopyrimidine + DNA
-
-
-
?
DNA containing formamidopyrimidine-guanine residues + H2O
4,6-diamino-5-formamidopyrimidine + DNA
-
-
-
?
DNA containing formamidopyrimidine-guanine residues + H2O
4,6-diamino-5-formamidopyrimidine + DNA
-
-
-
?
DNA containing formamidopyrimidine-guanine residues + H2O
4,6-diamino-5-formamidopyrimidine + DNA
-
-
-
?
DNA containing methylated formamidopyrimidine-guanine residues + H2O
2,6-diamino-4-hydroxy-5-(N-methyl)formamidopyrimidine + DNA
-
-
-
?
DNA containing methylated formamidopyrimidine-guanine residues + H2O
2,6-diamino-4-hydroxy-5-(N-methyl)formamidopyrimidine + DNA
-
-
-
?
DNA containing methylated formamidopyrimidine-guanine residues + H2O
2,6-diamino-4-hydroxy-5-(N-methyl)formamidopyrimidine + DNA
-
-
-
?
DNA containing methylated formamidopyrimidine-guanine residues + H2O
2,6-diamino-4-hydroxy-5-(N-methyl)formamidopyrimidine + DNA
-
-
-
?
DNA containing methylated formamidopyrimidine-guanine residues + H2O
2,6-diamino-4-hydroxy-5-(N-methyl)formamidopyrimidine + DNA
-
-
-
?
DNA containing ring-opened N7-methylguanine residues + H2O
2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine + DNA
-
-
-
-
?
DNA containing ring-opened N7-methylguanine residues + H2O
2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine + DNA
-
-
-
?
DNA containing ring-opened N7-methylguanine residues + H2O
2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine + DNA
-
-
-
?
DNA containing ring-opened N7-methylguanine residues + H2O
2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine + DNA
-
-
-
?
DNA containing ring-opened N7-methylguanine residues + H2O
2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine + DNA
-
-
-
-
?
DNA containing ring-opened N7-methylguanine residues + H2O
2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine + DNA
-
-
-
?
DNA containing ring-opened N7-methylguanine residues + H2O
2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine + DNA
-
-
-
-
?
DNA containing ring-opened N7-methylguanine residues + H2O
2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine + DNA
-
enzyme excises the secondary alkylation product of 7-methylguanine Fapy
-
?
DNA containing ring-opened N7-methylguanine residues + H2O
2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine + DNA
-
double-stranded DNA is preferred to single-stranded DNA
-
?
DNA containing ring-opened N7-methylguanine residues + H2O
2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine + DNA
-
dublex oligodeoxynucleotides containing Me-Fapy
-
?
DNA containing ring-opened N7-methylguanine residues + H2O
2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine + DNA
-
comparison of repair activities of human OGG1 and Escherichia coli Fpg, almost no paired base-dependent repair, effect of sequence context on repair efficiency, mechanism via Schiff base intermediate
treatment of me-Fapy with Fpg results in delta-elimination products
?
DNA containing ring-opened N7-methylguanine residues + H2O
2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine + DNA
23-oligomer
-
?
DNA containing ring-opened N7-methylguanine residues + H2O
2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine + DNA
-
less mendatory role of P-2 in 2,6-diamino-4-hydroxy-5-N-methyl-formamidopyrimidine-DNA glycosylase activity
-
-
?
DNA containing ring-opened N7-methylguanine residues + H2O
2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine + DNA
-
the C-8 keto group of 8-oxodeoxyguanine and the carbonyl moiety of formamidopyrimidine enable the enzyme to recognize and bind duplex DNA containing modified bases, mechanism
-
?
DNA containing ring-opened N7-methylguanine residues + H2O
2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine + DNA
-
amino terminal localization of the catalytic site
-
?
DNA containing ring-opened N7-methylguanine residues + H2O
2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine + DNA
-
-
-
-
?
DNA containing ring-opened N7-methylguanine residues + H2O
2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine + DNA
-
enzyme excises the secondary alkylation product of 7-methylguanine Fapy
-
?
DNA containing ring-opened N7-methylguanine residues + H2O
2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine + DNA
-
amino terminal localization of the catalytic site
-
?
DNA containing ring-opened N7-methylguanine residues + H2O
2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine + DNA
-
-
?
DNA containing tetrahydrofuran residues + H2O
tetrahydrofuran + DNA
-
-
-
?
DNA containing tetrahydrofuran residues + H2O
tetrahydrofuran + DNA
-
-
-
-
?
additional information
?
-
Fpg prefers thymine glycol, 5-hydroxycytosine, 5-hydroxyuracil, 5,6-dihydrothymine and 5,6-dihydrouracil, over 7,8-dihydro-8-oxoguanine, the oxidation products of 8-oxoG namely, Gh:C, Sp1:C and Sp2:C are the best substrates for the enzyme, single-stranded DNA is also a substrate, no activity with 8-oxoguanine, Fpg substrate specificity, overview
-
-
?
additional information
?
-
-
Fpg prefers thymine glycol, 5-hydroxycytosine, 5-hydroxyuracil, 5,6-dihydrothymine and 5,6-dihydrouracil, over 7,8-dihydro-8-oxoguanine, the oxidation products of 8-oxoG namely, Gh:C, Sp1:C and Sp2:C are the best substrates for the enzyme, single-stranded DNA is also a substrate, no activity with 8-oxoguanine, Fpg substrate specificity, overview
-
-
?
additional information
?
-
the enzyme does not excise 8-oxoguanine from DNA
-
-
?
additional information
?
-
-
the enzyme does not excise 8-oxoguanine from DNA
-
-
?
additional information
?
-
-
Fpg prefers thymine glycol, 5-hydroxycytosine, 5-hydroxyuracil, 5,6-dihydrothymine and 5,6-dihydrouracil, over 7,8-dihydro-8-oxoguanine, the oxidation products of 8-oxoG namely, Gh:C, Sp1:C and Sp2:C are the best substrates for the enzyme, single-stranded DNA is also a substrate, no activity with 8-oxoguanine, Fpg substrate specificity, overview
-
-
?
additional information
?
-
-
not: carbocyclic substrate analog of 8-oxo-7,8-dihydro-2-deoxyguanosine
-
-
?
additional information
?
-
-
no cleavage of 3-methyladenine, uracil, intact 7-methylguanine from DNA
-
-
?
additional information
?
-
-
not: dublex DNA containing a single tetrahydrofuran residue
-
-
?
additional information
?
-
-
human OGG1 and Escherichia coli Fpg are structurally unrelated enzymes with different catalytic residues, OGG1: Lys-249 and Asp-268 in the hairpin-helix-hairpin-GDP motif are involved in the glycosylase/AP lyase activity, Fpg: uses a proline residue in the N-terminal region for catalysis
-
-
?
additional information
?
-
-
not: DNA containing 8-oxo-7,8-dihydro-2-deoxyadenosine, single-stranded DNA, dublex DNA containing synthetic abasic sites, mismatches containing dG, unmodified DNA
-
-
?
additional information
?
-
-
base excision repair initiated by the enzyme is less effective in the first two days of growth and more effective later in stationary phase
-
-
?
additional information
?
-
-
enzyme mediates repair of lesions containing hydantoins in vivo
-
-
?
additional information
?
-
-
cross-linking of active center with a series of reactive oligonucleotide duplexes containing both a single 8-oxoguanine residue and an O-ethyl-substituted diphosphate internucleotide group results in identification of seven phosphate groups on both strands of the DNA duplex specifically interacting with nucleophilic amino acids of the enzyme. L56 of enzyme cross-links to the phosphate located 3' to the 8-oxoguanine residue
-
-
?
additional information
?
-
-
defective repair of 5-hydroxy-2-deoxycytidine in Cockayne syndrome cells is complementated by Escherichia coli formamidopyrimidine DNA glycosylase and endonuclease III
-
-
?
additional information
?
-
-
formamidopyrimidine-DNA N-glycosylase operates in the base excision repair pathway in bacteria by removing oxidized guanine bases from DNA and can also cleave the nascent or preformed abasic DNA by beta,delta-elimination. The cleaved product formation is initially reversible
-
-
?
additional information
?
-
-
FPG excises oxidatively damaged purines in the base excision repair pathway, overview
-
-
?
additional information
?
-
-
formamidopyrimidine-DNA N-glycosylase removes oxidized guanine bases from DNA. The cleaved product formation is initially reversible, it is followed by conformational changes in the enzyme and DNA molecules that represent the postchemical irreversible rate-limiting steps. The overall rate-limiting step of the enzymatic reaction seems to be the release of Fpg from its adduct with the 4-oxo-2-pentenal remnant of the deoxyribose moiety formed as a result of DNA strand cleavage by beta,delta-elmination, the initial chemical steps are fast and reversible. Catalytic mechanism, overview
-
-
?
additional information
?
-
-
FPG excises oxidatively damaged purines in the base excision repair pathway, it acts on DNA containing 5,6-dihydrouracil, 8-oxo-7,8-dihydroguanine, or on apurinic/apyrimidinic DNA base pairs, analysis of conformational dynamics of Fpg protein and DNA substrates, rate constants of conformational transitions, and intrinsic mechanism of recognition and excision of damaged bases in DNA, overview
-
-
?
additional information
?
-
-
formamidopyrimidine DNA glycosylase is specific for oxidized purines
-
-
?
additional information
?
-
-
the enzyme is more active towards oxidized purines than oxidized pyrimidines and has little to no activity toward DNA containing thymine glycols and osmium-tetroxide-treated DNA
-
-
?
additional information
?
-
-
analysis of acidity and proton affinity for a range of substrates such as 8-oxoguanine, 8-oxoadenine, 8-oxoinosine, 8-oxonebularine, formamidopyrimidine-guanine, 5-hydroxyuracil, and 5,6-dihydrothymine. The most favorable mechanism involves preprotonation of the O4', which results in the opening of the ribose ring
-
-
?
additional information
?
-
-
not: carbocyclic substrate analog of 8-oxo-7,8-dihydro-2-deoxyguanosine
-
-
?
additional information
?
-
-
the enzyme is more active towards oxidized purines than oxidized pyrimidines and has little to no activity toward DNA containing thymine glycols and osmium-tetroxide-treated DNA
-
-
?
additional information
?
-
not: DNA containing 2-hydroxyadenine, 1-N6 ethenoadenine, 3-N4 ethenocytosine, hypoxanthine, xanthine
-
-
?
additional information
?
-
not: DNA containing 2-hydroxyadenine, 1-N6 ethenoadenine, 3-N4 ethenocytosine, hypoxanthine, xanthine
-
-
?
additional information
?
-
-
cross-linking of active center with a series of reactive oligonucleotide duplexes containing both a single 8-oxoguanine residue and an O-ethyl-substituted diphosphate internucleotide group results in identification of eight phosphate groups on both strands of the DNA duplex specifically interacting with nucleophilic amino acids of the enzyme. L249 of enzyme cross-links to the phosphate located 3' to the 8-oxoguanine residue
-
-
?
additional information
?
-
-
treatment of cells with 4-(acetoxymethylnitrosamino)-1-(3-pyridyl)-1-butanone generates formamidopyrimidine glycosylase sensitive DNA sites with cell-type dependent differences in adduct frequency and time
-
-
?
additional information
?
-
8-oxo-7,8-dihydroguanine, i.e. 8-oxoGua, and 2,6-diamino-4-hydroxy-5-formamidopyrimidine, i.e. FapyGua, are premutagenic DNA lesions that appear in DNA damaged by reactive oxygen species of endogenous and environmental origin, and are excised from DNA by the enzyme. The fidelity of the 8-oxoGua repair system depends on discrimination between 8-oxoGua:Cyt and 8-oxoGua:Ade pairs by OGG1
-
-
?
additional information
?
-
-
8-oxo-7,8-dihydroguanine, i.e. 8-oxoGua, and 2,6-diamino-4-hydroxy-5-formamidopyrimidine, i.e. FapyGua, are premutagenic DNA lesions that appear in DNA damaged by reactive oxygen species of endogenous and environmental origin, and are excised from DNA by the enzyme. The fidelity of the 8-oxoGua repair system depends on discrimination between 8-oxoGua:Cyt and 8-oxoGua:Ade pairs by OGG1
-
-
?
additional information
?
-
-
NEIL1 is active on DNA lesions in ssDNA, particularly in the context of a single-stranded bubble in a duplex sequence
-
-
?
additional information
?
-
8-oxoguanine-DNA glycosylase, OGG1, efficiently removes mutagenic 8-oxo-7,8-dihydroguanine and 2,6-diamino-4-hydroxy-5-formamidopyrimidine when paired with cytosine in oxidatively damaged DNA. Excision of 8-oxoGua mispaired with adenine may lead to G to T transversions. Substrate specificity of wild-type and mutant enzymes, overview
-
-
?
additional information
?
-
-
8-oxoguanine-DNA glycosylase, OGG1, efficiently removes mutagenic 8-oxo-7,8-dihydroguanine and 2,6-diamino-4-hydroxy-5-formamidopyrimidine when paired with cytosine in oxidatively damaged DNA. Excision of 8-oxoGua mispaired with adenine may lead to G to T transversions. Substrate specificity of wild-type and mutant enzymes, overview
-
-
?
additional information
?
-
-
the enzyme is a DNA glycosylase/AP lyase specific for oxidized purines such as 8-oxo-7,8-dihydropurines and imidazole-ring opened purines (2,6-diamino-4-hydroxy-5-formamidopyrimidine and formamidopyrimidine derived from adenine)
-
-
?
additional information
?
-
-
MmuNeil3 is a bifunctional DNA glycosylase that recognizes spiroiminodihydantoin and guanidinohydantoin, as well as 2,6-diamino-4-hydroxy-5-formamidopyrimidine, and 4,6-diamino-5-formamidopyrimidine, in double-stranded substrates. Neil3 greatly reduces both the spontaneous mutation frequency and the level of 2,6-diamino-4-hydroxy-5-formamidopyrimidine in the DNA. Substrate specificity of MmuNeil3 in vivo, overview
-
-
?
additional information
?
-
-
NEIL1 is active on DNA lesions in ssDNA, particularly in the context of a single-stranded bubble in a duplex sequence
-
-
?
additional information
?
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in duplex DNA, mouse Neil3 recognizes the oxidized purines, spiroiminodihydantoin, guanidinohydantoin, 2,6-diamino-4-hydroxy-5-formamidopyrimidine, and 4,6-diamino-5-formamidopyrimidine, but not 8-oxo-7,8-dihydroguanine. Neil3 prefers lesions in single-stranded DNA and in bubble structures. Mouse Neil3 forms a Schiff base intermediate via its N-terminal valine, in contrast to other enzyme of the family that use proline as nucleophile
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the enzyme has no specificity for DNA containing 8-hydroxyguanine residues
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the mouse enzyme cannot initiate repair of aflatoxin B1-induced DNA base alkylation damage in either ds or ss DNA
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additional information
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the mouse enzyme cannot initiate repair of aflatoxin B1-induced DNA base alkylation damage in either ds or ss DNA
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additional information
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Mtb-Fpg1 removes formamidopyrimidine and 5-hydroxycytosine lesions, as well as 8-oxo-7,8-dihydroguanine opposite to C, T and G. Substrates are duplex DNA substrates containing a single 8oxoG opposite of C, A, G, T, 5-hydroxycytosine (5OHC):G, 5-hydroxyuracil (5OHU):G, DHU:G, U:A or U:G base pair
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additional information
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Mtb-Fpg1 removes formamidopyrimidine and 5-hydroxycytosine lesions, as well as 8-oxo-7,8-dihydroguanine opposite to C, T and G. Substrates are duplex DNA substrates containing a single 8oxoG opposite of C, A, G, T, 5-hydroxycytosine (5OHC):G, 5-hydroxyuracil (5OHU):G, DHU:G, U:A or U:G base pair
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additional information
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Mtb-Fpg1 removes formamidopyrimidine and 5-hydroxycytosine lesions, as well as 8-oxo-7,8-dihydroguanine opposite to C, T and G. Substrates are duplex DNA substrates containing a single 8oxoG opposite of C, A, G, T, 5-hydroxycytosine (5OHC):G, 5-hydroxyuracil (5OHU):G, DHU:G, U:A or U:G base pair
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additional information
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Mtb-Fpg1 removes formamidopyrimidine and 5-hydroxycytosine lesions, as well as 8-oxo-7,8-dihydroguanine opposite to C, T and G. Substrates are duplex DNA substrates containing a single 8oxoG opposite of C, A, G, T, 5-hydroxycytosine (5OHC):G, 5-hydroxyuracil (5OHU):G, DHU:G, U:A or U:G base pair
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