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3.2.1.176: cellulose 1,4-beta-cellobiosidase (reducing end)

This is an abbreviated version!
For detailed information about cellulose 1,4-beta-cellobiosidase (reducing end), go to the full flat file.

Word Map on EC 3.2.1.176

Reaction

2 cellohexaose + 2 H2O = 2 cellotriose +

cellotetraose
+
cellobiose

Synonyms

1,4-beta-D-glucan cellobiohydrolase I, 1,4-beta-D-glucan-cellobiohydrolase I, CBH, CBH I, CBH Ib, CBH-1, CBH1, CBH2, Cbh3, Cbh7B, CbhA, CBHI, CbhI.1, Cel48A, Cel48C, Cel48S, Cel48S cellulase, Cel48Y cellulase, Cel6A, Cel7A, Cel7B, Cel7D, Cel9B, cellobiohydrolase, cellobiohydrolase 1, cellobiohydrolase A, cellobiohydrolase CelS, cellobiohydrolase class I, cellobiohydrolase I, cellobiohydrolase I-I, cellobiohydrolase I-II, cellobiohydrolase II, cellobiohydrolase II-I, Celluclast, Cellulase SS, CelO, celS, CelSS, CelY, Ct-Cel5F, DDB0202233, DICPUDRAFT_151874, EG I, EgxS, endoglucanase, endoglucanase SS, exo-1,4-beta-glucanase, exo-acting cellulase, exo-beta-1,4-glucanase, exocellulase, exocellulase E4, exocellulase E6, exoglucanase, exoglucanase S, exoglucanase-1, GH7 CBH, More, reducing end acting processive exocellulase, reducing end-acting CBH, Tfu_1959, ThCel7A, THITE_62596, Tr-Cel7A

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.176 cellulose 1,4-beta-cellobiosidase (reducing end)

Temperature Stability

Temperature Stability on EC 3.2.1.176 - cellulose 1,4-beta-cellobiosidase (reducing end)

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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
50 - 60
-
unstable at temperatures lower than 50°C, retains about 80% of its activity after a 30 min incubation at 50°C and is completely inactivated after a 30 min pre-incubation at or above 60°C
50 - 65
-
the enzyme is stable at 50°C at least for 3 h. At 60 and 65°C, the enzyme half-life times are in the range of 40-43 min and 11-13 min, respectively
50 - 70
the enzyme has a half-life of 60 min at 70°C and after incubation for 72 h retains more than 80% of enzymatic activity at 60°C and 100% at 50°C, respectively
55
pH 6.0, stable for 24 h
55 - 70
thermal unfolding behavior of Th Cel7A is found to be a two-step process at pH 5.0. The first step leads to the formation of molten globule-like states between 55°C and 65°C, followed by a second step that was due to the unfolding of the molten globule-like state at temperatures above 70°C
62
melting temperature
62 - 69
the melting temperature is 62.5°C. The wild type enzyme shows half-lives of 19.9, 9.3 and 8.6 min at 62°C, 66°C, and 69°C, respectively
64.2 - 74.7
-
melting tempeartures, recombinant expression in Saccharomyces cerevisiae and in Neurospora crassa. The Cel7A expressed in Neurospora crassa has a by 10°C higher melting temperature and higher specific activity than the Cel7A expressed in Saccharomyces cerevisiae
66.9
-
melting temperature
68
melting temperature, wild-type
70.5
melting temperature, mutant carrying a cellulose-binding module
71
melting temperature, mutant G4C/M70C
72
melting temperature, fusion protein with CBM2 from Cellulomonas fimi xylanase 10A
73
melting temperature, wild-type
74
melting temperature, fusion protein with CBM1 from Hypocrea jecorina Cel7A
75
melting temperature, fusion protein with CBM3 derived from Clostridium thermocellum cellulosomal-scaffolding protein CipA
75.5
melting temperature, fusion protein with CBM1 from Hypocrea jecorina Cel7A containing an additional S-S bridge in the catalytic module
77
melting temperature, fusion protein with CBM3 derived from Clostridium thermocellum cellulosomal-scaffolding protein CipA containing an additional S-S bridge in the catalytic module
additional information