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2.9 - 104
2,4-dinitrophenyl beta-lactoside
0.11
2-chloro-4-nitrophenyl beta-D-cellobioside
catalyticdomain, pH 5.0, 37°C
0.017 - 0.74
2-chloro-4-nitrophenyl beta-D-lactoside
0.0063 - 12.8
2-chloro-4-nitrophenyl beta-lactoside
0.0153 - 0.3167
2-chloro-4-nitrophenyl-beta-D-lactoside
0.74
2-chloro-4-nitrophenyl-beta-lactoside
pH 5.0, 50°C
1.7
4-bromophenyl beta-lactoside
-
pH 5.7, 37°C, wild-type enzyme
0.35
4-methylumbelliferyl beta-D-cellobioside
pH 5.0, 55°C
0.08 - 0.49
4-methylumbelliferyl beta-D-lactopyranoside
0.055 - 1.35
4-methylumbelliferyl beta-D-lactoside
0.0073 - 47
4-methylumbelliferyl beta-lactoside
0.083 - 1.15
4-methylumbelliferyl-beta-D-lactoside
0.1 - 0.39
4-nitrophenyl beta-D-cellobioside
0.238 - 0.6
4-nitrophenyl beta-D-lactopyranoside
0.19 - 0.51
4-nitrophenyl beta-D-lactoside
0.00066 - 3.5
4-nitrophenyl beta-lactoside
0.39
4-nitrophenyl D-cellobioside
pH 5.0, 50°C
13.4
4-nitrophenyl-beta-D-lactoside
pH 5.0, 50°C
1.8 - 5.1
amorphous cellulose
-
2.6 - 2.8
bacterial cellulose
-
2.4
bacterial microcrystalline cellulose
-
in 50 mM sodium acetate with 2 mM CaCl2 and pH 5.0 at 25°C
-
2.9
2,4-dinitrophenyl beta-lactoside
-
pH 5.7, 37°C, mutant D214N
12.2
2,4-dinitrophenyl beta-lactoside
-
pH 5.7, 37°C, mutant E217Q
104
2,4-dinitrophenyl beta-lactoside
-
pH 5.7, 37°C, wild-type enzyme
0.017
2-chloro-4-nitrophenyl beta-D-lactoside
wild-type, pH 6.0, 22°C
0.02
2-chloro-4-nitrophenyl beta-D-lactoside
mutant S290T, pH 6.0, 22°C
0.165
2-chloro-4-nitrophenyl beta-D-lactoside
mutant G4C/M70C/S290T, pH 6.0, 22°C
0.182
2-chloro-4-nitrophenyl beta-D-lactoside
mutant G4C/M70C, pH 6.0, 22°C
0.74
2-chloro-4-nitrophenyl beta-D-lactoside
50 mM sodium acetate buffer, pH 5.0, at 50°C
0.0063
2-chloro-4-nitrophenyl beta-lactoside
-
pH 5.7, 37°C, mutant E212Q
0.035
2-chloro-4-nitrophenyl beta-lactoside
-
pH 5.7, 37°C, mutant E217Q
0.15
2-chloro-4-nitrophenyl beta-lactoside
-
pH 5.7, 37°C, mutant D214N
12.8
2-chloro-4-nitrophenyl beta-lactoside
-
pH 5.7, 37°C, wild-type enzyme
0.0153
2-chloro-4-nitrophenyl-beta-D-lactoside
-
Cel7B wild-type expressed in Saccharomyces cerevisiae, at 22°C, pH 6.0
0.0165
2-chloro-4-nitrophenyl-beta-D-lactoside
-
Cel7B mutant S290T/G4C/M70C expressed in Saccharomyces cerevisiae, at 22°C, pH 6.0
0.0167
2-chloro-4-nitrophenyl-beta-D-lactoside
-
Cel7B wild-type expressed in Trichoderma reesei, at 22°C, pH 6.0
0.01817
2-chloro-4-nitrophenyl-beta-D-lactoside
-
Cel7B mutant G4C/M70C expressed in Saccharomyces cerevisiae, at 22°C, pH 6.0
0.0205
2-chloro-4-nitrophenyl-beta-D-lactoside
-
Cel7B mutant S290T expressed in Saccharomyces cerevisiae, at 22°C, pH 6.0
0.0283
2-chloro-4-nitrophenyl-beta-D-lactoside
in 50 mM sodium phosphate buffer, pH 5.7, at 22°C
0.043
2-chloro-4-nitrophenyl-beta-D-lactoside
-
in 50 mM sodium phosphate buffer, pH 5.7, at 22°C
0.0467
2-chloro-4-nitrophenyl-beta-D-lactoside
in 50 mM sodium phosphate buffer, pH 5.7, at 22°C
0.3167
2-chloro-4-nitrophenyl-beta-D-lactoside
Thermochaetoides thermophila
in 50 mM sodium phosphate buffer, pH 5.7, at 22°C
0.08
4-methylumbelliferyl beta-D-lactopyranoside
mutant G4C/M70C, pH 6.0, 22°C
0.11
4-methylumbelliferyl beta-D-lactopyranoside
mutant S290T, pH 6.0, 22°C
0.12
4-methylumbelliferyl beta-D-lactopyranoside
mutant G4C/M70C/S290T, pH 6.0, 22°C
0.14
4-methylumbelliferyl beta-D-lactopyranoside
wild-type, pH 6.0, 22°C
0.33
4-methylumbelliferyl beta-D-lactopyranoside
mutant enzyme W40A, at pH 5.0 and 27°C
0.49
4-methylumbelliferyl beta-D-lactopyranoside
wild type enzyme, at pH 5.0 and 27°C
0.055
4-methylumbelliferyl beta-D-lactoside
pH 5.0, 22°C, wild-type
0.31
4-methylumbelliferyl beta-D-lactoside
pH 5.0, 45°C, wild-type
0.33
4-methylumbelliferyl beta-D-lactoside
pH 5.0, 27°C, recombinant mutant W40A enzyme
0.361
4-methylumbelliferyl beta-D-lactoside
pH 5.0, 27°C, recombinant mutant W40A enzyme catalytic domain
0.415
4-methylumbelliferyl beta-D-lactoside
pH 5.0, 27°C, recombinant wild-type enzyme catalytic domain
0.435
4-methylumbelliferyl beta-D-lactoside
pH 5.0, 45°C, fusion protein with CBM1 from Hypocrea jecorina Cel7A containing an additional S-S bridge in the catalytic module
0.492
4-methylumbelliferyl beta-D-lactoside
pH 5.0, 27°C, recombinant wild-type enzyme
0.65
4-methylumbelliferyl beta-D-lactoside
pH 5.0, 22°C, fusion protein with CBM1 from Hypocrea jecorina Cel7A containing an additional S-S bridge in the catalytic module
1.35
4-methylumbelliferyl beta-D-lactoside
pH 5.0, 60°C, wild-type
0.0073
4-methylumbelliferyl beta-lactoside
-
pH 5.7, 37°C, mutant E217Q
0.063
4-methylumbelliferyl beta-lactoside
-
pH 5.7, 37°C, mutant E212Q
2.3
4-methylumbelliferyl beta-lactoside
-
pH 5.7, 37°C, mutant D214N
47
4-methylumbelliferyl beta-lactoside
-
pH 5.7, 37°C, wild-type enzyme
0.083
4-methylumbelliferyl-beta-D-lactoside
-
Cel7B mutant G4C/M70C expressed in Saccharomyces cerevisiae, at 22°C, pH 6.0
0.1067
4-methylumbelliferyl-beta-D-lactoside
-
Cel7B wild-type expressed in Saccharomyces cerevisiae, at 22°C, pH 6.0
0.1083
4-methylumbelliferyl-beta-D-lactoside
-
Cel7B mutant S290T expressed in Saccharomyces cerevisiae, at 22°C, pH 6.0
0.12
4-methylumbelliferyl-beta-D-lactoside
-
Cel7B mutant S290T/G4C/M70C expressed in Saccharomyces cerevisiae, at 22°C, pH 6.0
0.143
4-methylumbelliferyl-beta-D-lactoside
-
Cel7B wild-type expressed in Trichoderma reesei, at 22°C, pH 6.0
0.1883
4-methylumbelliferyl-beta-D-lactoside
in 50 mM sodium acetate buffer, pH 5.0, at 22°C
0.325
4-methylumbelliferyl-beta-D-lactoside
in 50 mM sodium acetate buffer, pH 5.0, at 22°C
0.4783
4-methylumbelliferyl-beta-D-lactoside
-
in 50 mM sodium acetate buffer, pH 5.0, at 22°C
1.15
4-methylumbelliferyl-beta-D-lactoside
Thermochaetoides thermophila
in 50 mM sodium acetate buffer, pH 5.0, at 22°C
0.1
4-nitrophenyl beta-D-cellobioside
catalyticdomain, pH 5.0, 37°C
0.112
4-nitrophenyl beta-D-cellobioside
at 50°C in 50 mM sodium acetate (pH 5.0)
0.39
4-nitrophenyl beta-D-cellobioside
50 mM sodium acetate buffer, pH 5.0, at 50°C
0.238
4-nitrophenyl beta-D-lactopyranoside
-
at pH 4.0 and 55°C
0.27
4-nitrophenyl beta-D-lactopyranoside
at pH 5.0 and 40°C
0.273
4-nitrophenyl beta-D-lactopyranoside
at pH 4.0 and 60°C
0.6
4-nitrophenyl beta-D-lactopyranoside
at pH 5.0 and 40°C
0.19
4-nitrophenyl beta-D-lactoside
catalyticdomain, pH 5.0, 37°C
0.27
4-nitrophenyl beta-D-lactoside
pH 5.0, 45°C, recombinant protein carrying Hypocrea jecorina carbohydrate-binding module and linker
0.51
4-nitrophenyl beta-D-lactoside
pH 5.0, 45°C, recombinant protein carrying Hypocrea jecorina carbohydrate-binding module and linker
0.00066
4-nitrophenyl beta-lactoside
-
pH 5.7, 37°C, mutant E217Q
0.0041
4-nitrophenyl beta-lactoside
-
pH 5.7, 37°C, mutant E212Q
0.042
4-nitrophenyl beta-lactoside
-
pH 5.7, 37°C, mutant D214N
0.78
4-nitrophenyl beta-lactoside
-
at pH 5.0 and 25°C
3.5
4-nitrophenyl beta-lactoside
-
pH 5.7, 37°C, wild-type enzyme
1.8
amorphous cellulose
-
in 50 mM sodium acetate, pH 5.0, at 30°C
-
2
amorphous cellulose
-
in 50 mM sodium acetate, pH 5.0, at 30°C
-
5.1
amorphous cellulose
-
in 50 mM sodium acetate with 2 mM CaCl2 and pH 5.0 at 25°C
-
0.87
Avicel
at pH 5.0 and 25°C
-
4.75
Avicel
-
in 50 mM sodium acetate with 2 mM CaCl2 and pH 5.0 at 25°C
-
2.6
bacterial cellulose
-
in 50 mM sodium acetate, pH 5.0, at 30°C
-
2.8
bacterial cellulose
-
in 50 mM sodium acetate, pH 5.0, at 30°C
-