3.2.1.176: cellulose 1,4-beta-cellobiosidase (reducing end)
This is an abbreviated version!
For detailed information about cellulose 1,4-beta-cellobiosidase (reducing end), go to the full flat file.
Word Map on EC 3.2.1.176
-
3.2.1.176
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crew
-
subsystem
-
mission
-
hydroponic
-
cellulosome
-
thermocellum
-
bioregenerative
-
life-support
-
cellulases
-
lunar
-
long-duration
-
inedible
-
degradation
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breadboard
-
avicel
-
dockerin
-
moon
-
synthesis
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biotechnology
-
biofuel production
-
analysis
-
paper production
- 3.2.1.176
-
crew
-
subsystem
-
mission
-
hydroponic
- cellulosome
- thermocellum
-
bioregenerative
-
life-support
- cellulases
-
lunar
-
long-duration
-
inedible
- degradation
-
breadboard
- avicel
-
dockerin
-
moon
- synthesis
- biotechnology
- biofuel production
- analysis
- paper production
Reaction
2 cellohexaose
+
2 H2O
=
2 cellotriose
+
Synonyms
1,4-beta-D-glucan cellobiohydrolase I, 1,4-beta-D-glucan-cellobiohydrolase I, CBH, CBH I, CBH Ib, CBH-1, CBH1, CBH2, Cbh3, Cbh7B, CbhA, CBHI, CbhI.1, Cel48A, Cel48C, Cel48S, Cel48S cellulase, Cel48Y cellulase, Cel6A, Cel7A, Cel7B, Cel7D, Cel9B, cellobiohydrolase, cellobiohydrolase 1, cellobiohydrolase A, cellobiohydrolase CelS, cellobiohydrolase class I, cellobiohydrolase I, cellobiohydrolase I-I, cellobiohydrolase I-II, cellobiohydrolase II, cellobiohydrolase II-I, Celluclast, Cellulase SS, CelO, celS, CelSS, CelY, Ct-Cel5F, DDB0202233, DICPUDRAFT_151874, EG I, EgxS, endoglucanase, endoglucanase SS, exo-1,4-beta-glucanase, exo-acting cellulase, exo-beta-1,4-glucanase, exocellulase, exocellulase E4, exocellulase E6, exoglucanase, exoglucanase S, exoglucanase-1, GH7 CBH, More, reducing end acting processive exocellulase, reducing end-acting CBH, Tfu_1959, ThCel7A, THITE_62596, Tr-Cel7A
ECTree
3.2.1.176 cellulose 1,4-beta-cellobiosidase (reducing end)Advanced search results
results (
results (Specific Activity
Specific Activity on EC 3.2.1.176 - cellulose 1,4-beta-cellobiosidase (reducing end)
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SPECIFIC ACTIVITY [µmol/min/mg] 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
0.028
0.036
0.038
0.039
0.041
0.044
0.047
0.117
0.17
0.19
0.2
0.22
0.51
pH 5.0, 22°C, fusion protein with CBM1 from Hypocrea jecorina Cel7A
0.52
pH 5.0, 22°C, fusion protein with CBM3 derived from Clostridium thermocellum cellulosomal-scaffolding protein CipA
0.53
pH 5.0, 22°C, fusion protein with CBM1 from Hypocrea jecorina Cel7A containing an additional S-S bridge in the catalytic module
0.56
pH 5.0, 22°C, fusion protein with CBM2 from Cellulomonas fimi xylanase 10A
0.57
pH 5.0, 22°C, fusion protein with CBM3 derived from Clostridium thermocellum cellulosomal-scaffolding protein CipA containing an additional S-S bridge in the catalytic module
275000
substrate phosphoric acid swollen cellulose, pH 5.0, 37°C
additional information
0.028
-
substrate 4-nitrophenyl beta-D-lactoside, wild-type, pH 5, 50°C
0.028
-
wild type enzyme, with 4-nitrophenyl beta-D-lactoside as substrate, at pH 4.0 and 40°C
0.036
-
substrate 4-nitrophenyl beta-D-lactoside, mutant N194A, pH 5, 50°C
0.036
-
mutant enzyme N194A, with 4-nitrophenyl beta-D-lactoside as substrate, at pH 4.0 and 40°C
0.038
-
substrate 4-nitrophenyl beta-D-lactoside, mutant N45A, pH 5, 50°C
0.038
-
mutant enzyme N45A, with 4-nitrophenyl beta-D-lactoside as substrate, at pH 4.0 and 40°C
0.039
-
substrate 4-nitrophenyl beta-D-lactoside, mutant N388A, pH 5, 50°C
0.039
-
mutant enzyme N388A, with 4-nitrophenyl beta-D-lactoside as substrate, at pH 4.0 and 40°C
0.041
-
mutant enzyme N388A, with beta-glucan as substrate, at pH 4.0 and 40°C
0.044
-
mutant enzyme N194A, with beta-glucan as substrate, at pH 4.0 and 40°C
0.047
-
wild type enzyme, with beta-glucan as substrate, at pH 4.0 and 40°C
0.117
-
mutant enzyme N45A, with beta-glucan as substrate, at pH 4.0 and 40°C
0.17
-
mutant enzyme N194A, with Avicel as substrate, at pH 4.0 and 40°C
0.19
-
mutant enzyme N388A, with Avicel as substrate, at pH 4.0 and 40°C
0.2
-
wild type enzyme, with Avicel as substrate, at pH 4.0 and 40°C
0.22
-
mutant enzyme N45A, with Avicel as substrate, at pH 4.0 and 40°C
additional information
-
melting temperatures, recombinant expression in Saccharomyces cerevisiae and in Neurospora crassa. The Cel7A expressed in Neurospora crassa has a by 10°C higher melting temperature and higher specific activity than the Cel7A expressed in Saccharomyces cerevisiae
additional information
-
the processive action of reducing-end-specific Trichoderma reesei cellobiohydrolase Cel7A on derivatized cellulose model substrates allows to perform an active-site titration, which in turn can be used to quantify the processivity of the enzyme
