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Literature summary for 3.2.1.176 extracted from

  • Momeni, M.H.; Goedegebuur, F.; Hansson, H.; Karkehabadi, S.; Askarieh, G.; Mitchinson, C.; Larenas, E.A.; Stahlberg, J.; Sandgren, M.
    Expression, crystal structure and cellulase activity of the thermostable cellobiohydrolase Cel7A from the fungus Humicola grisea var. thermoidea (2014), Acta Crystallogr. Sect. D, 70, 2356-2366.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.176

Application

Application Comment Organism
degradation comparison of the activity w ith Humicola jecorina Cel7A reveals a much higher hydrolytic rate for Humicola grisea Cel7A at both 65°C (4.8fold higher initial rate) and 38°C (3.3fold higher) [Humicola] grisea var. thermoidea

Crystallization (Commentary)

Crystallization (Comment) Organism
protein lacking the C-terminal linker-CBM1 part, yielding crystals of space group P212121 with two protein molecules, chains A and B, in the asymmetric unit, to 1.8 A resolution. The catalytic triad consisting of Glu213 (nucleophile), Asp215 and Glu218 (acid/base), and the tryptophan platforms at subsites -7, -4, -2 and +1 are highly conserved [Humicola] grisea var. thermoidea
structure of catalytic module, 1.8 A resolution. The crystal structure of the enzyme reveals considerable flexibility of the active-site-defining loop regions [Humicola] grisea var. thermoidea

Organism

Organism UniProt Comment Textmining
[Humicola] grisea var. thermoidea P15828
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylumbelliferyl-beta-D-lactoside + H2O
-
 [Humicola] grisea var. thermoidea 4-methylumbelliferol + D-lactose
-
 ?
cellulose + H2O substrate acid-swollen cellulose [Humicola] grisea var. thermoidea cellobiose + ?
-
 ?
corn stover + H2O
-
 [Humicola] grisea var. thermoidea cellobiose + ?
-
 ?
pretreated corn stover + H2O
-
 [Humicola] grisea var. thermoidea ? 14% conversion, 24 h, 65°C, best performance of the tested enzymes ?

Synonyms

Synonyms Comment Organism
CBH-1
-
 [Humicola] grisea var. thermoidea
Cel7A
-
 [Humicola] grisea var. thermoidea
exoglucanase-1
-
 [Humicola] grisea var. thermoidea

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
72.5
-
 melting temperature [Humicola] grisea var. thermoidea