3.2.1.176: cellulose 1,4-beta-cellobiosidase (reducing end) This is an abbreviated version! For detailed information about cellulose 1,4-beta-cellobiosidase (reducing end), go to the full flat file .
Reaction
2 cellohexaose
+
2 H2O
=
2 cellotriose
+
cellotetraose +
cellobiose
Synonyms 1,4-beta-D-glucan cellobiohydrolase I, 1,4-beta-D-glucan-cellobiohydrolase I, CBH, CBH I, CBH Ib, CBH-1, CBH1, CBH2, Cbh3, Cbh7B, CbhA, CBHI, CbhI.1, Cel48A, Cel48C, Cel48S, Cel48S cellulase, Cel48Y cellulase, Cel6A, Cel7A, Cel7B, Cel7D, Cel9B, cellobiohydrolase, cellobiohydrolase 1, cellobiohydrolase A, cellobiohydrolase CelS, cellobiohydrolase class I, cellobiohydrolase I, cellobiohydrolase I-I, cellobiohydrolase I-II, cellobiohydrolase II, cellobiohydrolase II-I, Celluclast, Cellulase SS, CelO, celS, CelSS, CelY, Ct-Cel5F, DDB0202233, DICPUDRAFT_151874, EG I, EgxS, endoglucanase, endoglucanase SS, exo-1,4-beta-glucanase, exo-acting cellulase, exo-beta-1,4-glucanase, exocellulase, exocellulase E4, exocellulase E6, exoglucanase, exoglucanase S, exoglucanase-1, GH7 CBH, More, reducing end acting processive exocellulase, reducing end-acting CBH, Tfu_1959, ThCel7A, THITE_62596, Tr-Cel7A
ECTree
Purification
Purification on EC 3.2.1.176 - cellulose 1,4-beta-cellobiosidase (reducing end)
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ammonium sulfate precipitation and MonoQ column chromatography
-
ammonium sulfate precipitation and phenyl Sepharose resin column chromatography
ammonium sulfate precipitation and Source 15Phe column chromatography
ammonium sulfate precipitation, phenyl Sepharose 6 column chromatography Source 15Q resin column chromatography, and Superdex 75 gel filtration
-
ammonium sulfate precipitation, phenyl Sepharose column chromatography, Source 15Q column chromatography, and Superdex 75 gel filtration
ammonium sulfate precipitation, Source 15Q column chromatography, and Source 15 isopropyl column chromatography
-
anion exchange column chromatography and gel filtration
Bio-Gel P-6 gel filtration, DEAE-Sepharose column chromatography and phenyl Sepharose column chromatography
DEAE Sepharose FF column chromatography
-
HiTrap phenyl column chromatography and Superdex 200 gel filtration
Ni-NTA column chromatography
-
Ni2+ affinity column chromatography
Ni2+ Sepharose column chromatography and Superdex S200 gel filtration
-
on affinity column and by gel filtration
phenyl Sepharose column chromatography, Source 15Q column chromatography, and Sephadex G-25 gel filtration
-
Q Sepharose column chromatography and DEAE-Sephadex gel filtration
Q-Sepharose Fast-Flow column chromatography and Mono Q column chromatography
-
Q-Trap column chromatography
recombinant -type and mutant W40A enzymes from Trichoderma reesei strain ALKO 3413 culture filtrate by gel filtration, anion exchange, hydrophobic interaction, and affinity chromatography
recombinant C-terminally His6-tagged enzyme from Saccharomyces cerevisiae by anion exchange chromaatography and dialysis, recombinant enzyme from Neurospora crassa by ammonium sulfate fractionation, gel filtration, anion exchange chromatography and dialysis
-
recombinant enzyme and truncated versions, homogeneity
recombinant His6-tagged enzyme from Escherichia coli (BL21) by cation exchange and hydrophobic interaction chromatography, followed by another step of cation exchange and anion exchange chromatography and gel filtration
Sephacryl S-200 gel filtration
-
Superdex 75 gel filtration and Mono-Q column chromatography
-
the enzyme and catalytic domain alone are each expressed in and purified from Streptomyces lividans
wild-type and mutants purified with one-step purification using gel filtration
-
-
-
ammonium sulfate precipitation and Source 15Phe column chromatography
-
ammonium sulfate precipitation and Source 15Phe column chromatography
ammonium sulfate precipitation, phenyl Sepharose column chromatography, Source 15Q column chromatography, and Superdex 75 gel filtration
ammonium sulfate precipitation, phenyl Sepharose column chromatography, Source 15Q column chromatography, and Superdex 75 gel filtration
HiTrap phenyl column chromatography and Superdex 200 gel filtration
-
HiTrap phenyl column chromatography and Superdex 200 gel filtration
-
homogeneity
-
Ni2+ affinity column chromatography
-
Ni2+ affinity column chromatography
-
Ni2+ affinity column chromatography
Thermochaetoides thermophila
-
Ni2+ affinity column chromatography
-
Ni2+ affinity column chromatography
-
on affinity column and by gel filtration
-
on affinity column and by gel filtration
on affinity column and by gel filtration
on affinity column and by gel filtration
Thermochaetoides thermophila