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3.2.1.176: cellulose 1,4-beta-cellobiosidase (reducing end)

This is an abbreviated version!
For detailed information about cellulose 1,4-beta-cellobiosidase (reducing end), go to the full flat file.

Word Map on EC 3.2.1.176

Reaction

2 cellohexaose + 2 H2O = 2 cellotriose +

cellotetraose
+
cellobiose

Synonyms

1,4-beta-D-glucan cellobiohydrolase I, 1,4-beta-D-glucan-cellobiohydrolase I, CBH, CBH I, CBH Ib, CBH-1, CBH1, CBH2, Cbh3, Cbh7B, CbhA, CBHI, CbhI.1, Cel48A, Cel48C, Cel48S, Cel48S cellulase, Cel48Y cellulase, Cel6A, Cel7A, Cel7B, Cel7D, Cel9B, cellobiohydrolase, cellobiohydrolase 1, cellobiohydrolase A, cellobiohydrolase CelS, cellobiohydrolase class I, cellobiohydrolase I, cellobiohydrolase I-I, cellobiohydrolase I-II, cellobiohydrolase II, cellobiohydrolase II-I, Celluclast, Cellulase SS, CelO, celS, CelSS, CelY, Ct-Cel5F, DDB0202233, DICPUDRAFT_151874, EG I, EgxS, endoglucanase, endoglucanase SS, exo-1,4-beta-glucanase, exo-acting cellulase, exo-beta-1,4-glucanase, exocellulase, exocellulase E4, exocellulase E6, exoglucanase, exoglucanase S, exoglucanase-1, GH7 CBH, More, reducing end acting processive exocellulase, reducing end-acting CBH, Tfu_1959, ThCel7A, THITE_62596, Tr-Cel7A

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.176 cellulose 1,4-beta-cellobiosidase (reducing end)

Purification

Purification on EC 3.2.1.176 - cellulose 1,4-beta-cellobiosidase (reducing end)

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate precipitation and MonoQ column chromatography
-
ammonium sulfate precipitation and phenyl Sepharose resin column chromatography
ammonium sulfate precipitation and Source 15Phe column chromatography
ammonium sulfate precipitation, phenyl Sepharose 6 column chromatography Source 15Q resin column chromatography, and Superdex 75 gel filtration
-
ammonium sulfate precipitation, phenyl Sepharose column chromatography, Source 15Q column chromatography, and Superdex 75 gel filtration
ammonium sulfate precipitation, Source 15Q column chromatography, and Source 15 isopropyl column chromatography
-
anion exchange column chromatography and gel filtration
Bio-Gel P-6 gel filtration, DEAE-Sepharose column chromatography and phenyl Sepharose column chromatography
CBH I and CBH II
-
DEAE Sepharose FF column chromatography
-
HiTrap phenyl column chromatography and Superdex 200 gel filtration
homogeneity
Ni-NTA column chromatography
-
Ni2+ affinity column chromatography
Ni2+ Sepharose column chromatography and Superdex S200 gel filtration
-
on affinity column and by gel filtration
phenyl Sepharose column chromatography, Source 15Q column chromatography, and Sephadex G-25 gel filtration
-
Q Sepharose column chromatography and DEAE-Sephadex gel filtration
Q-Sepharose Fast-Flow column chromatography and Mono Q column chromatography
-
Q-Trap column chromatography
recombinant -type and mutant W40A enzymes from Trichoderma reesei strain ALKO 3413 culture filtrate by gel filtration, anion exchange, hydrophobic interaction, and affinity chromatography
recombinant C-terminally His6-tagged enzyme from Saccharomyces cerevisiae by anion exchange chromaatography and dialysis, recombinant enzyme from Neurospora crassa by ammonium sulfate fractionation, gel filtration, anion exchange chromatography and dialysis
-
recombinant enzyme and truncated versions, homogeneity
recombinant His6-tagged enzyme from Escherichia coli (BL21) by cation exchange and hydrophobic interaction chromatography, followed by another step of cation exchange and anion exchange chromatography and gel filtration
Sephacryl S-200 gel filtration
-
Superdex 75 gel filtration and Mono-Q column chromatography
-
the enzyme and catalytic domain alone are each expressed in and purified from Streptomyces lividans
wild-type and mutants
-
wild-type and mutants purified with one-step purification using gel filtration
-