Literature summary for 3.2.1.176 extracted from

Brunecky, R.; Alahuhta, M.; Bomble, Y.J.; Xu, Q.; Baker, J.O.; Ding, S.Y.; Himmel, M.E.; Lunin, V.V.
Structure and function of the Clostridium thermocellum cellobiohydrolase A X1-module repeat: enhancement through stabilization of the CbhA complex (2012), Acta Crystallogr. Sect. D, 68, 292-299.

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Cloned(Commentary)

Cloned (Comment)	Organism
recombinant expression of His6-tagged enzyme in Escherichia coli (BL21)	Acetivibrio thermocellus

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified recombinant enzyme, sitting drop vapor diffusion method, mixing of 0.001 ml each of 80 mg/ml protein, in 20 mM Tris, pH 7.0, with 100 mM NaCl, and 1.9 M sodium malonate, pH 6.0, or with 1.9 M sodium malonate, pH 6.5, 20% v/v ethylene glycol, 50 mM KI. In a second method, 15 mg/ml protein in 20 mM acetic acid buffer, pH 5.0, with 100 mM NaCl is mixed with 0.2 M ammonium iodide, 20% w/v PEG 3350, pH 6.2, X-ray diffraction structure determination and analysis at 1.7 A resolution, single isomorphous replacement with anomalous signal from iodine and molecular-replacement	Acetivibrio thermocellus

Crystallization (Comment)

Organism

purified recombinant enzyme, sitting drop vapor diffusion method, mixing of 0.001 ml each of 80 mg/ml protein, in 20 mM Tris, pH 7.0, with 100 mM NaCl, and 1.9 M sodium malonate, pH 6.0, or with 1.9 M sodium malonate, pH 6.5, 20% v/v ethylene glycol, 50 mM KI. In a second method, 15 mg/ml protein in 20 mM acetic acid buffer, pH 5.0, with 100 mM NaCl is mixed with 0.2 M ammonium iodide, 20% w/v PEG 3350, pH 6.2, X-ray diffraction structure determination and analysis at 1.7 A resolution, single isomorphous replacement with anomalous signal from iodine and molecular-replacement

Acetivibrio thermocellus

Organism

Organism	UniProt	Comment	Textmining
Acetivibrio thermocellus	A3DCH2	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant His6-tagged enzyme from Escherichia coli (BL21) by cation exchange and hydrophobic interaction chromatography, followed by another step of cation exchange and anion exchange chromatography and gel filtration	Acetivibrio thermocellus

Synonyms

Synonyms	Comment	Organism
CbhA	-	Acetivibrio thermocellus
cellobiohydrolase A	-	Acetivibrio thermocellus

General Information

General Information	Comment	Organism
metabolism	cellulosomal enzyme systems utilize self-assembled scaffolded multimodule enzymes to deconstruct biomass, structure-function relationships governing the action of the large cellulosomal enzyme complex	Acetivibrio thermocellus
additional information	crystal structures of the two X1 modules from the enzyme CbhA	Acetivibrio thermocellus