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3.2.1.176: cellulose 1,4-beta-cellobiosidase (reducing end)

This is an abbreviated version!
For detailed information about cellulose 1,4-beta-cellobiosidase (reducing end), go to the full flat file.

Word Map on EC 3.2.1.176

Reaction

2 cellohexaose + 2 H2O = 2 cellotriose +

cellotetraose
+
cellobiose

Synonyms

1,4-beta-D-glucan cellobiohydrolase I, 1,4-beta-D-glucan-cellobiohydrolase I, CBH, CBH I, CBH Ib, CBH-1, CBH1, CBH2, Cbh3, Cbh7B, CbhA, CBHI, CbhI.1, Cel48A, Cel48C, Cel48S, Cel48S cellulase, Cel48Y cellulase, Cel6A, Cel7A, Cel7B, Cel7D, Cel9B, cellobiohydrolase, cellobiohydrolase 1, cellobiohydrolase A, cellobiohydrolase CelS, cellobiohydrolase class I, cellobiohydrolase I, cellobiohydrolase I-I, cellobiohydrolase I-II, cellobiohydrolase II, cellobiohydrolase II-I, Celluclast, Cellulase SS, CelO, celS, CelSS, CelY, Ct-Cel5F, DDB0202233, DICPUDRAFT_151874, EG I, EgxS, endoglucanase, endoglucanase SS, exo-1,4-beta-glucanase, exo-acting cellulase, exo-beta-1,4-glucanase, exocellulase, exocellulase E4, exocellulase E6, exoglucanase, exoglucanase S, exoglucanase-1, GH7 CBH, More, reducing end acting processive exocellulase, reducing end-acting CBH, Tfu_1959, ThCel7A, THITE_62596, Tr-Cel7A

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.176 cellulose 1,4-beta-cellobiosidase (reducing end)

Cloned

Cloned on EC 3.2.1.176 - cellulose 1,4-beta-cellobiosidase (reducing end)

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cloned in Escherichia coli
cloned into Escherichia coli and Streptomyces lividans
expressed as an endoglucanase-cellobiohydrolase fusion protein in Saccharomyces cerevisiae, Yarrowia lipolytica, and Lipomyces starkeyi
-
expressed in Aspergillus niger
-
expressed in Aspergillus niger variant awamori AP4
expressed in Aspergillus oryzae
expressed in Aspergillus oryzae and Aspergillus niger
expressed in Escherichia coli BL21(DE3) cells
-
expressed in Escherichia coli strain DH5alpha
expressed in Escherichia coli strains BL21, Origami, and Origami B
-
expressed in Escherichia coli XL-1 Blue cells
expressed in Fusarium venenatum
-
expressed in Penicillium canescens strain PCA10
-
expressed in Pichia pastoris Pichia Pink strain2
-
expressed in Saccharomyces cerevisiae
expressed in Saccharomyces cerevisiae strain CEN.PK102-3A
expressed in Saccharomyces cerevisiae strain NY179
-
expressed in Saccharomyces cerevisiae strain YDR483W
expressed in Trichoderma reesei
expressed in yeast strain YDR483W BY4742
-
expression in Aspergillus oryzae and Aspergillus niger
expression in Escherichia coli
expression in Penicillium canescens PCA10
-
expression in Pichia pastoris
expression in Saccharomyces cerevisiae
expression in Zea mays
folding and/or post-translation modification of heterologous enzyme in Yarrowia lipolytica cannot completely mimic that in the original source strain. Trichoderma reesei/Talatomyces emersonii chimeric cellobiohydrolase I containing the catalytic domain from Talaromyces emersonii and the linker and carbohydrate-binding module from Trichoderma reesei
functional expression of Cel9B and Cel48C in Escherichia coli
-
gene cbh1, DNA and amino acid sequence determination
-
gene cel7A, heterologous expression of wild-type and mutant enzymes in Aspergillus oryzae
gene cel7A, recombinant expression of wild-type and mutant W40A enzymes under the control of its own promoter in Trichoderma reesei strain ALKO 3413 lacking the genes encoding for endogenous Cel7A and Cel7B
gene cel7AF, recombinant expression of extracellular C-terminally FLAG-tagged enzyme with its native signal peptide in Saccharomyces cerevisiae strain CEN.PK102-3, the enzyme is secreted to the culture medium
native enzyme and truncated versions
recombinant expression of C-terminally His6-tagged enzyme in Saccharomyces cerevisiae, and expression in Neurospora crassa using an Neurospora crassa codon bias and Neurospora crassa CBHI signal peptide and cloned in pCSR1:GPD vector, which directs gene integration to the csr-1 locus. Gene expression is promoted by the constitutive Myceliophthora thermophila gpdA promoter. The Cel7A expressed in Neurospora crassa has a higher melting temperature and higher specific activity than the Cel7A expressed in Saccharomyces cerevisiae. The underlying cause of this disparity is the lack of N-terminal glutamine cyclization in the Cel7A expressed in Saccharomyces cerevisiae. Treating the enzyme in vitro with glutaminyl cyclase improves the properties of Cel7A expressed in Saccharomyces cerevisiae to match those of Cel7A expressed in Neurospora crassa
-
recombinant expression of His6-tagged enzyme in Escherichia coli (BL21)
wild-type and mutants expressed in Saccharomyces cerevisiae strain INVSc1. Cel7B wild-type and a two-module version containing as a C-terminal fusion the linker and cellulose-binding module (CBM) of Trichoderma reesei Cel7A expressed in Trichoderma reesei strain A36
-
wild-type Cel7A expressed in Trichoderma reesei
wild-type Cel7A, Cel7A genetically linked to the linker and carbohydrate-binding module of Trichoderma reesei Cel7A or to the linker and carbohydrate-binding module of Chaetomium thermophilum Cel7A expressed in Trichoderma reesei
wild-type Cel7B expressed in Trichoderma reesei