3.2.1.140: lacto-N-biosidase
This is an abbreviated version!
For detailed information about lacto-N-biosidase, go to the full flat file.
Word Map on EC 3.2.1.140
-
3.2.1.140
-
oligosaccharides
-
bifidum
-
lacto-n-tetraose
-
milk
-
infant
-
glycoside
-
gal
-
glcnac
-
lactose
-
lacto-n-fucopentaose
-
triantennary
-
lacto-n-triose
-
gut-associated
-
lacto-n-neotetraose
-
bifidobacterial
-
tetrasaccharide
-
transglycosylation
-
glycosidases
-
n-acetyllactosamine
-
breast-fed
-
galactosyl
-
2-aminopyridine
-
commensal
-
sialyl
-
microbiota
-
subsite
-
analysis
-
synthesis
- 3.2.1.140
- oligosaccharides
- bifidum
- lacto-n-tetraose
- milk
- infant
- glycoside
-
gal
- glcnac
- lactose
- lacto-n-fucopentaose
-
triantennary
- lacto-n-triose
-
gut-associated
- lacto-n-neotetraose
-
bifidobacterial
- tetrasaccharide
-
transglycosylation
- glycosidases
- n-acetyllactosamine
-
breast-fed
-
galactosyl
- 2-aminopyridine
-
commensal
-
sialyl
- microbiota
-
subsite
- analysis
- synthesis
Reaction
Synonyms
BbLNBase, BLLJ_1505, BLLJ_1506, GH20 lacto-N-biosidase, lacto-N-biosidase, lacto-N-biosidase (Streptomyces strain 142), LNBase, LnbB, LnbX
ECTree
3.2.1.140 lacto-N-biosidaseAdvanced search results
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results (Substrates Products
Substrates Products on EC 3.2.1.140 - lacto-N-biosidase
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SUBSTRATE 
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
2'-fucosyllactose + H2O
lactose + fucose
-
Fuc-alpha-(1->2)-Gal-beta-(1->4)-Glc
-
-
?
4-methylumbelliferyl 2-acetamido-2-deoxy-3-O-(beta-D-galactopyranosyl)-D-glucopyranoside + H2O
4-methylumbelliferol + 2-acetamido-2-deoxy-3-O-(beta-D-galactopyranosyl)-D-glucopyranose
-
-
-
?
4-nitrophenyl-GalNAc-beta-(1->3)-GlcNAc + H2O
4-nitrophenol + GalNAc-beta-(1->3)-GlcNAc
-
-
-
?
4-nitrophenyl-lacto-N-bioside + H2O
4-nitrophenol + lacto-N-biose
-
-
-
?
D-Gal-(1,3)-beta-GlcNAc-(1,3)-beta-D-Gal-(1,4)-beta-D-Glc-pyridylamine + H2O
?
lacto-N-tetraose-pyridylamine, no activity on alpha-linked disaccharides, beta-linked p-nitrophenyl monosaccharides such as 4-nitrophenyl beta-D-N-acetylglucosamine and 4-nitrophenyl beta-D-N-acetyl-D-galactosamine, no activity on ganglioside GA1 structure with beta-linked galacto-N-biose, no activity on fucosylated forms of lacto-N-tetraose or lacto-N-neotetraose
-
-
?
D-Gal-(1,3)-beta-GlcNAc-(1,3)-beta-D-Gal-(1,4)-D-Glc + H2O
lactose + D-galactosyl-beta-1,3-N-acetyl-D-glucosamine
Fuc-alpha-(1->2)-Gal-beta-(1->3)-GalNAc-beta-(1->3)-Gal-alpha-(1->4)-Gal-beta-(1->4)-Glc + H2O
Fuc-alpha-(1->2)-Gal-beta-(1->3)-GalNAc + Gal-alpha-(1->4)-Gal-beta-(1->4)-Glc
i.e. Globo H
-
-
?
Fuc-alpha-(1->2)-Gal-beta-(1->3)-GlcNAc-beta-(1->3)-Gal-beta-(1->4)-Glc + H2O
Fuc-alpha-(1->2)-Gal-beta-(1->3)-GlcNAc + Gal-beta-(1->4)-Glc
i.e. LNFP I
-
-
?
Gal-beta-(1->3)-GalNAc-beta-(1->3)-Gal-alpha-(1->4)-Gal-beta-(1->4)-Glc + H2O
Gal-beta-(1->3)-GalNAc + Gal-alpha-(1->4)-Gal-beta-(1->4)-Glc
Gal-beta-(1->3)-GalNAc-beta-(1->4)-Gal-beta-(1->4)-Glc + H2O
Gal-beta-(1->3)-GlcNAc + Gal-beta-(1->4)-Glc
i.e. GA1
-
-
?
Gal-beta-(1->3)-GlcNAc-beta-(1->3)-Gal-beta-(1->4)-Glc + H2O
Gal-beta-(1->3)-GlcNAc + Gal-beta-(1->4)-Glc
lacto-N-difucohexaose + H2O
lactose + Fuc-alpha-(1->2)-Gal-beta-(1->3)[Fuc-alpha-(1->4)]GlcNAc
-
Fuc-alpha-(1->2)Gal-beta-(1->3)[Fuc-alpha-(1->4)]GlcNAc-beta-(1->3)-Gal-beta-(1->4)-Glc
-
-
?
lacto-N-hexaose + H2O
lactose + lacto-N-tetraose
Gal-beta-(1->3)-GlcNAc-beta-(1->3)-[Gal-beta-(1->4)-GlcNAc-beta-(1->6)]-beta-(1->4)-Glc
-
-
?
lacto-N-tetraose + H2O
lacto-N-biose I + lactose
-
-
-
?
p-nitrophenyl-Gal-(1,3)-beta-GalNAc + H2O
?
30% enzyme activity compared to p-nitrophenyl-Gal-(1,3)-beta-GlcNAc
-
-
?
pyridylamine-oligosaccharides which have type 1 chains at the nonreducing terminus + H2O
lacto-N-biose + ?
sialyllacto-N-tetraose a + H2O
lactose + Fuc-alpha-(1->2)-Gal-beta-(1->3)[Fuc-alpha-(1->4)]GlcNAc
4-nitrophenyl-beta-lacto-N-bioside + H2O
4-nitrophenol + lacto-N-biose
-
-
-
?
4-nitrophenyl-beta-lacto-N-bioside + H2O
4-nitrophenol + lacto-N-biose
-
-
-
?
4-nitrophenyl-Gal-beta-(1->3)-GalNAc + H2O
4-nitrophenol + Gal-beta-(1->3)-GalNAc
-
-
-
?
4-nitrophenyl-Gal-beta-(1->3)-GalNAc + H2O
4-nitrophenol + Gal-beta-(1->3)-GalNAc
-
-
-
?
D-Gal-(1,3)-beta-GlcNAc-(1,3)-beta-D-Gal-(1,4)-D-Glc + H2O
lactose + D-galactosyl-beta-1,3-N-acetyl-D-glucosamine
lacto-N-tetraose, major component of human milk oligosaccharides
lacto-N-biose I
-
?
D-Gal-(1,3)-beta-GlcNAc-(1,3)-beta-D-Gal-(1,4)-D-Glc + H2O
lactose + D-galactosyl-beta-1,3-N-acetyl-D-glucosamine
-
lacto-N-tetraose, major component of human milk oligosaccharides
lacto-N-biose I
-
?
Gal-beta-(1->3)-GalNAc-beta-(1->3)-Gal-alpha-(1->4)-Gal-beta-(1->4)-Glc + H2O
Gal-beta-(1->3)-GalNAc + Gal-alpha-(1->4)-Gal-beta-(1->4)-Glc
i.e. Gb5
-
-
?
Gal-beta-(1->3)-GalNAc-beta-(1->3)-Gal-alpha-(1->4)-Gal-beta-(1->4)-Glc + H2O
Gal-beta-(1->3)-GalNAc + Gal-alpha-(1->4)-Gal-beta-(1->4)-Glc
i.e. Gb5
-
-
?
Gal-beta-(1->3)-GlcNAc-beta-(1->3)-Gal-beta-(1->4)-Glc + H2O
Gal-beta-(1->3)-GlcNAc + Gal-beta-(1->4)-Glc
i.e. LNT
-
-
?
Gal-beta-(1->3)-GlcNAc-beta-(1->3)-Gal-beta-(1->4)-Glc + H2O
Gal-beta-(1->3)-GlcNAc + Gal-beta-(1->4)-Glc
i.e. LNT
-
-
?
Galbeta1-3GlcNAcbeta-p-nitrophenol + H2O
Galbeta1-3GlcNAc + p-nitrophenol
-
-
-
-
?
Galbeta1-3GlcNAcbeta-p-nitrophenol + H2O
Galbeta1-3GlcNAc + p-nitrophenol
-
-
-
-
?
Galbeta1-3GlcNAcbeta-p-nitrophenol + lactose
Galbeta1-3GlcNAcbeta1-3Galbeta1-4Glc + ?
-
-
Galbeta1-3GlcNAcbeta1-3Galbeta1-4Glc, i.e. lacto-neotetraose
-
?
Galbeta1-3GlcNAcbeta-p-nitrophenol + lactose
Galbeta1-3GlcNAcbeta1-3Galbeta1-4Glc + ?
-
-
Galbeta1-3GlcNAcbeta1-3Galbeta1-4Glc, i.e. lacto-neotetraose
-
?
lacto-N-fucopentaose I + H2O
lactose + Fuc-alpha-(1->2)-Gal-beta-(1->3)-GlcNAc
-
Fuc-alpha-(1->2)-Gal-beta-(1->3)-GlcNAc-beta-(1->3)-Gal-beta-(1->4)-Glc
-
-
?
lacto-N-fucopentaose I + H2O
lactose + Fuc-alpha-(1->2)-Gal-beta-(1->3)-GlcNAc
Fuc-alpha-(1->2)-Gal-beta-(1->3)-GlcNAc-beta-(1->3)-Gal-beta-(1->4)-Glc
-
-
?
lacto-N-fucopentaose I + H2O
lactose + Fuc-alpha-(1->2)-Gal-beta-(1->3)-GlcNAc
Fuc-alpha-(1->2)-Gal-beta-(1->3)-GlcNAc-beta-(1->3)-Gal-beta-(1->4)-Glc
-
-
?
lacto-N-tetraose + H2O
lacto-N-biose + lactose
-
-
-
?
lacto-N-tetraose + H2O
lactose + lacto-N-biose
-
Gal-beta-(1->3)-GlcNAc-beta-(1->3)-Gal-beta-(1->4)-Glc
-
-
?
lacto-N-tetraose + H2O
lactose + lacto-N-biose
-
lacto-N-tetraose is a major component of human milk oligosaccharides, substrate used to examine the LnbB activities in various bacterial strains
-
-
?
lacto-N-tetraose + H2O
lactose + lacto-N-biose
-
lacto-N-tetraose is a major component of human milk oligosaccharides, substrate used to examine the LnbB activities in various bacterial strains
-
-
?
lacto-N-tetraose + H2O
lactose + lacto-N-biose
Gal-beta-(1->3)-GlcNAc-beta-(1->3)-Gal-beta-(1->4)-Glc
-
-
?
lacto-N-tetraose + H2O
lactose + lacto-N-biose
Gal-beta-(1->3)-GlcNAc-beta-(1->3)-Gal-beta-(1->4)-Glc
-
-
?
p-nitrophenyl-Gal-(1,3)-beta-GlcNAc + H2O
?
lacto-N-biosyl-beta-p-nitrophenyl, more genereal lacto-N-tetraose with 1-alcanols (methanol, ethanol, 1-propanol, 1-butanol) or p-nitrophenyl-beta-lacto-N-biose + lactose with enzyme
-
-
?
p-nitrophenyl-Gal-(1,3)-beta-GlcNAc + H2O
?
-
30% relative activity compared to p-nitrophenyl-lacto-N-bioside
-
-
?
p-nitrophenyl-lacto-N-bioside + H2O
p-nitrophenol + lacto-N-biose
-
most active substrate
-
-
?
p-nitrophenyl-lacto-N-bioside + H2O
p-nitrophenol + lacto-N-biose
-
-
-
?
p-nitrophenyl-lacto-N-bioside + H2O
p-nitrophenol + lacto-N-biose
-
-
-
?
p-nitrophenyl-lacto-N-bioside + H2O
p-nitrophenol + lacto-N-biose
-
-
-
-
?
pyridylamine-oligosaccharides which have type 1 chains at the nonreducing terminus + H2O
lacto-N-biose + ?
-
-
from N-acetyllactosamine type of triantennary sugar chain with type 1 structure and N-avcetyllactosamine type of biantennary sugar chain is produced
?
pyridylamine-oligosaccharides which have type 1 chains at the nonreducing terminus + H2O
lacto-N-biose + ?
-
no reaction with pyridylamine-oligosaccharides which contain only type 2 chains. No reaction with oligosaccharides in which the non-reducing terminal Gal or the penultimate GlcNAc are replaced by fucose or sialic acid are
-
-
?
pyridylamine-oligosaccharides which have type 1 chains at the nonreducing terminus + H2O
lacto-N-biose + ?
-
no reaction with pyridylamine-oligosaccharides which contain only type 2 chains. No reaction with oligosaccharides in which the non-reducing terminal Gal or the penultimate GlcNAc are replaced by fucose or sialic acid are
-
-
?
pyridylamine-oligosaccharides which have type 1 chains at the nonreducing terminus + H2O
lacto-N-biose + ?
-
-
from N-acetyllactosamine type of triantennary sugar chain with type 1 structure and N-avcetyllactosamine type of biantennary sugar chain is produced
?
sialyllacto-N-tetraose a + H2O
lactose + Fuc-alpha-(1->2)-Gal-beta-(1->3)[Fuc-alpha-(1->4)]GlcNAc
Neu5Ac-alpha-(2->3)-Gal-beta-(1->3)-GlcNAc-beta-(1->3)-Gal-beta-(1->4)-Gal
-
-
?
sialyllacto-N-tetraose a + H2O
lactose + Fuc-alpha-(1->2)-Gal-beta-(1->3)[Fuc-alpha-(1->4)]GlcNAc
Neu5Ac-alpha-(2->3)-Gal-beta-(1->3)-GlcNAc-beta-(1->3)-Gal-beta-(1->4)-Gal
-
-
?
additional information
?
-
-
human milk oligosaccharides are substrates and sole carbon source for Bifidobacterium bifidum colonizing the human intestinal tract. More than 130 types of human milk oligosaccharides have been isolated with the most abundant being lacto-N-tetraose, lacto-N-fucopentaose I, lacto-N-difucohexaose, and 2'-fucosyllactose
-
-
?
additional information
?
-
human milk oligosaccharides are substrates and sole carbon source for Bifidobacterium bifidum colonizing the human intestinal tract. More than 130 types of human milk oligosaccharides have been isolated with the most abundant being lacto-N-tetraose, lacto-N-fucopentaose I, lacto-N-difucohexaose, and 2'-fucosyllactose
-
-
?
additional information
?
-
-
human milk oligosaccharides are substrates and sole carbon source for Bifidobacterium bifidum colonizing the human intestinal tract. More than 130 types of human milk oligosaccharides have been isolated with the most abundant being lacto-N-tetraose, lacto-N-fucopentaose I, lacto-N-difucohexaose, and 2'-fucosyllactose
-
-
?
additional information
?
-
the enzyme releases a disaccharide
-
-
?
additional information
?
-
-
the enzyme releases a disaccharide
-
-
?
additional information
?
-
LnbB, a member of the glycoside hydrolase family 20 isolated from Bifidobacterium bifidum, releases Galbeta1-3GalNAc (GNB) from Gb5 and GA1 oligosaccharides. Substrate specificity, overview. Glycan-scavenging activity of LnbB, i.e. the activity of the enzyme towards the oligosaccharides of globo- and ganglio-series sphingolipids. Activity of GH20 LnbB towards Gb5 oligosaccharide is significantly low. No activity with Fuc-alpha-(1->2)-Gal-beta-(1->3)-GalNAc-beta-(1->3)-Gal-alpha-(1->4)-Gal-beta-(1->4)-Glc (Globo H) and Fuc-alpha-(1->2)Gal-beta-(1->3)-GlcNAc-beta-(1->3)-Gal-beta-(1->4)-Glc (LNFP I)
-
-
?
additional information
?
-
-
no activity on beta-linked p-nitrophenyl-monosaccharides, ganglioside GA1 structure with a beta-linked galacto-N-biose, fucosylated forms of lacto-N-tetraose or lacto-N-neoteraose
-
-
?
additional information
?
-
-
the recombinant enzyme shows substrate preference for the unmodified beta-linked lacto-biose I structure
-
-
?
additional information
?
-
the native substrate are human milk oligosaccharides. Among the different structures, four molecular species, namely 2'-fucosyllactose, lacto-N-tetraose, lacto-N-fucopentaose I, and lacto-N-difucohexaose I, are most abundantly present and comprise more than 50% of the total oligosaccharides, unless the milk is derived from non-secretor or Lewis-negative subjects
-
-
?
additional information
?
-
-
the native substrate are human milk oligosaccharides. Among the different structures, four molecular species, namely 2'-fucosyllactose, lacto-N-tetraose, lacto-N-fucopentaose I, and lacto-N-difucohexaose I, are most abundantly present and comprise more than 50% of the total oligosaccharides, unless the milk is derived from non-secretor or Lewis-negative subjects
-
-
?
additional information
?
-
the purified enzyme, which consists of LnbX only, hydrolyzes via a retaining mechanism the GlcNAcbeta-(1->3)-Gal linkage in lacto-N-tetraose, lacto-N-fucopentaose I, and sialyllacto-N-tetraose a. Inactive against lacto-N-neotetraose, lacto-N-triose II, lacto-N-fucopentaose II, and sialyllacto-N-tetraose b, substrate specificity, overview
-
-
?
additional information
?
-
-
the purified enzyme, which consists of LnbX only, hydrolyzes via a retaining mechanism the GlcNAcbeta-(1->3)-Gal linkage in lacto-N-tetraose, lacto-N-fucopentaose I, and sialyllacto-N-tetraose a. Inactive against lacto-N-neotetraose, lacto-N-triose II, lacto-N-fucopentaose II, and sialyllacto-N-tetraose b, substrate specificity, overview
-
-
?
additional information
?
-
the native substrate are human milk oligosaccharides. Among the different structures, four molecular species, namely 2'-fucosyllactose, lacto-N-tetraose, lacto-N-fucopentaose I, and lacto-N-difucohexaose I, are most abundantly present and comprise more than 50% of the total oligosaccharides, unless the milk is derived from non-secretor or Lewis-negative subjects
-
-
?
additional information
?
-
the purified enzyme, which consists of LnbX only, hydrolyzes via a retaining mechanism the GlcNAcbeta-(1->3)-Gal linkage in lacto-N-tetraose, lacto-N-fucopentaose I, and sialyllacto-N-tetraose a. Inactive against lacto-N-neotetraose, lacto-N-triose II, lacto-N-fucopentaose II, and sialyllacto-N-tetraose b, substrate specificity, overview
-
-
?
additional information
?
-
LnbX, a non-classified member of the glycoside hydrolase family, isolated from Bifidobacterium longum subsp. longum, liberates galacto-N-biose (GNB: Gal-beta-(1->3)-GalNAc) and 2'-fucosyl GNB (a type-4 trisaccharide) from Gb5 pentasaccharide and globo H hexasaccharide, respectively. Substrate specificity, overview. Glycan-scavenging activity of LnbX, i.e. the activity of the enzyme towards the oligosaccharides of globo- and ganglio-series sphingolipids. No activity with Gal-beta-(1->3)-GalNAc-beta-(1->4)-Gal-beta-(1->4)-Glc
-
-
?
additional information
?
-
-
no reaction with lacto-N-neotetraose, lacto-N-triose, sialyl lacto-N-tetraose, lacto-N-fucopentaose I, II, or III, asialo-GM1 tetrasaccharide, poly-N-acetyllactosamine
-
-
?
additional information
?
-
-
no reaction with lacto-N-neotetraose, lacto-N-triose, sialyl lacto-N-tetraose, lacto-N-fucopentaose I, II, or III, asialo-GM1 tetrasaccharide, poly-N-acetyllactosamine
-
-
?
