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Literature summary for 3.2.1.140 extracted from

  • Gotoh, A.; Katoh, T.; Sugiyama, Y.; Kurihara, S.; Honda, Y.; Sakurama, H.; Kambe, T.; Ashida, H.; Kitaoka, M.; Yamamoto, K.; Katayama, T.
    Novel substrate specificities of two lacto-N-biosidases towards beta-linked galacto-N-biose-containing oligosaccharides of globo H, Gb5, and GA1 (2015), Carbohydr. Res., 408, 18-24 .
    View publication on PubMed
Search for more literature for 3.2.1.140

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Bifidobacterium bifidum
recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Bifidobacterium longum subsp. Longum

Inhibitors

Inhibitors Comment Organism Structure
additional information no inhibition by Gal nor GlcNAc Bifidobacterium bifidum
additional information no inhibition by Gal nor GlcNAc Bifidobacterium longum subsp. Longum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.005
-
 4-nitrophenyl-Gal-beta-(1->3)-GalNAc pH 6.0, 30°C Bifidobacterium longum subsp. Longum
0.0088
-
 Gal-beta-(1->3)-GalNAc-beta-(1->3)-Gal-alpha-(1->4)-Gal-beta-(1->4)-Glc pH 6.0, 30°C Bifidobacterium bifidum
0.12
-
 4-nitrophenyl-beta-lacto-N-bioside pH 6.0, 30°C Bifidobacterium longum subsp. Longum
0.4
-
 Gal-beta-(1->3)-GlcNAc-beta-(1->3)-Gal-beta-(1->4)-Glc pH 6.0, 25°C Bifidobacterium longum subsp. Longum
2.5
-
 Gal-beta-(1->3)-GalNAc-beta-(1->3)-Gal-alpha-(1->4)-Gal-beta-(1->4)-Glc pH 6.0, 30°C Bifidobacterium longum subsp. Longum
15
-
 Fuc-alpha-(1->2)-Gal-beta-(1->3)-GlcNAc-beta-(1->3)-Gal-beta-(1->4)-Glc pH 6.0, 25°C Bifidobacterium longum subsp. Longum
67
-
 Gal-beta-(1->3)-GlcNAc-beta-(1->3)-Gal-beta-(1->4)-Glc pH 6.0, 25°C Bifidobacterium bifidum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
lacto-N-tetraose + H2O Bifidobacterium bifidum
-
 lacto-N-biose + lactose
-
 ?
lacto-N-tetraose + H2O Bifidobacterium longum subsp. Longum
-
 lacto-N-biose I + lactose
-
 ?

Organism

Organism UniProt Comment Textmining
Bifidobacterium bifidum B3TLD6
-
-
Bifidobacterium longum subsp. Longum A0A024QYS6
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) Bifidobacterium bifidum
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) Bifidobacterium longum subsp. Longum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl-beta-lacto-N-bioside + H2O
-
 Bifidobacterium bifidum 4-nitrophenol + lacto-N-biose
-
 ?
4-nitrophenyl-beta-lacto-N-bioside + H2O
-
 Bifidobacterium longum subsp. Longum 4-nitrophenol + lacto-N-biose
-
 ?
4-nitrophenyl-Gal-beta-(1->3)-GalNAc + H2O
-
 Bifidobacterium bifidum 4-nitrophenol + Gal-beta-(1->3)-GalNAc
-
 ?
4-nitrophenyl-Gal-beta-(1->3)-GalNAc + H2O
-
 Bifidobacterium longum subsp. Longum 4-nitrophenol + Gal-beta-(1->3)-GalNAc
-
 ?
Fuc-alpha-(1->2)-Gal-beta-(1->3)-GalNAc-beta-(1->3)-Gal-alpha-(1->4)-Gal-beta-(1->4)-Glc + H2O i.e. Globo H Bifidobacterium longum subsp. Longum Fuc-alpha-(1->2)-Gal-beta-(1->3)-GalNAc + Gal-alpha-(1->4)-Gal-beta-(1->4)-Glc
-
 ?
Fuc-alpha-(1->2)-Gal-beta-(1->3)-GlcNAc-beta-(1->3)-Gal-beta-(1->4)-Glc + H2O i.e. LNFP I Bifidobacterium longum subsp. Longum Fuc-alpha-(1->2)-Gal-beta-(1->3)-GlcNAc + Gal-beta-(1->4)-Glc
-
 ?
Gal-beta-(1->3)-GalNAc-beta-(1->3)-Gal-alpha-(1->4)-Gal-beta-(1->4)-Glc + H2O i.e. Gb5 Bifidobacterium bifidum Gal-beta-(1->3)-GalNAc + Gal-alpha-(1->4)-Gal-beta-(1->4)-Glc
-
 ?
Gal-beta-(1->3)-GalNAc-beta-(1->3)-Gal-alpha-(1->4)-Gal-beta-(1->4)-Glc + H2O i.e. Gb5 Bifidobacterium longum subsp. Longum Gal-beta-(1->3)-GalNAc + Gal-alpha-(1->4)-Gal-beta-(1->4)-Glc
-
 ?
Gal-beta-(1->3)-GalNAc-beta-(1->4)-Gal-beta-(1->4)-Glc + H2O i.e. GA1 Bifidobacterium bifidum Gal-beta-(1->3)-GlcNAc + Gal-beta-(1->4)-Glc
-
 ?
Gal-beta-(1->3)-GlcNAc-beta-(1->3)-Gal-beta-(1->4)-Glc + H2O i.e. LNT Bifidobacterium bifidum Gal-beta-(1->3)-GlcNAc + Gal-beta-(1->4)-Glc
-
 ?
Gal-beta-(1->3)-GlcNAc-beta-(1->3)-Gal-beta-(1->4)-Glc + H2O i.e. LNT Bifidobacterium longum subsp. Longum Gal-beta-(1->3)-GlcNAc + Gal-beta-(1->4)-Glc
-
 ?
lacto-N-tetraose + H2O
-
 Bifidobacterium bifidum lacto-N-biose + lactose
-
 ?
lacto-N-tetraose + H2O
-
 Bifidobacterium longum subsp. Longum lacto-N-biose I + lactose
-
 ?
additional information LnbB, a member of the glycoside hydrolase family 20 isolated from Bifidobacterium bifidum, releases Galbeta1-3GalNAc (GNB) from Gb5 and GA1 oligosaccharides. Substrate specificity, overview. Glycan-scavenging activity of LnbB, i.e. the activity of the enzyme towards the oligosaccharides of globo- and ganglio-series sphingolipids. Activity of GH20 LnbB towards Gb5 oligosaccharide is significantly low. No activity with Fuc-alpha-(1->2)-Gal-beta-(1->3)-GalNAc-beta-(1->3)-Gal-alpha-(1->4)-Gal-beta-(1->4)-Glc (Globo H) and Fuc-alpha-(1->2)Gal-beta-(1->3)-GlcNAc-beta-(1->3)-Gal-beta-(1->4)-Glc (LNFP I) Bifidobacterium bifidum ?
-
 ?
additional information LnbX, a non-classified member of the glycoside hydrolase family, isolated from Bifidobacterium longum subsp. longum, liberates galacto-N-biose (GNB: Gal-beta-(1->3)-GalNAc) and 2'-fucosyl GNB (a type-4 trisaccharide) from Gb5 pentasaccharide and globo H hexasaccharide, respectively. Substrate specificity, overview. Glycan-scavenging activity of LnbX, i.e. the activity of the enzyme towards the oligosaccharides of globo- and ganglio-series sphingolipids. No activity with Gal-beta-(1->3)-GalNAc-beta-(1->4)-Gal-beta-(1->4)-Glc Bifidobacterium longum subsp. Longum ?
-
 ?

Synonyms

Synonyms Comment Organism
LnbB
-
 Bifidobacterium bifidum
LnbX
-
 Bifidobacterium longum subsp. Longum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Bifidobacterium bifidum
30
-
 assay at Bifidobacterium longum subsp. Longum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.29
-
 Gal-beta-(1->3)-GalNAc-beta-(1->3)-Gal-alpha-(1->4)-Gal-beta-(1->4)-Glc pH 6.0, 30°C Bifidobacterium bifidum
0.84
-
 Gal-beta-(1->3)-GalNAc-beta-(1->3)-Gal-alpha-(1->4)-Gal-beta-(1->4)-Glc pH 6.0, 30°C Bifidobacterium longum subsp. Longum
1.5
-
 4-nitrophenyl-Gal-beta-(1->3)-GalNAc pH 6.0, 30°C Bifidobacterium longum subsp. Longum
4.9
-
 4-nitrophenyl-Gal-beta-(1->3)-GalNAc pH 6.0, 30°C Bifidobacterium bifidum
14
-
 Fuc-alpha-(1->2)-Gal-beta-(1->3)-GlcNAc-beta-(1->3)-Gal-beta-(1->4)-Glc pH 6.0, 25°C Bifidobacterium longum subsp. Longum
16
-
 4-nitrophenyl-beta-lacto-N-bioside pH 6.0, 30°C Bifidobacterium bifidum
42
-
 Gal-beta-(1->3)-GlcNAc-beta-(1->3)-Gal-beta-(1->4)-Glc pH 6.0, 25°C Bifidobacterium bifidum
88
-
 4-nitrophenyl-beta-lacto-N-bioside pH 6.0, 30°C Bifidobacterium longum subsp. Longum
110
-
 Gal-beta-(1->3)-GlcNAc-beta-(1->3)-Gal-beta-(1->4)-Glc pH 6.0, 25°C Bifidobacterium longum subsp. Longum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 assay at Bifidobacterium bifidum
6
-
 assay at Bifidobacterium longum subsp. Longum

General Information

General Information Comment Organism
evolution LnbB is a member of the glycoside hydrolase family 20, GH20 Bifidobacterium bifidum
evolution LnbX is a non-classified member of the glycoside hydrolase family Bifidobacterium longum subsp. Longum
physiological function lacto-N-biosidase is a key enzyme that degrades lacto-N-tetraose, a main component of human milk oligosaccharides (HMOs, the third-most abundant solid component in human milk), into lacto-N-biose I and lactose. . Enzymatic release of beta-linked GNB from natural substrates, these unique activities may play a role in modulating the microbial composition in the gut ecosystem Bifidobacterium bifidum
physiological function lacto-N-biosidase is a key enzyme that degrades lacto-N-tetraose, a main component of human milk oligosaccharides (HMOs, the third-most abundant solid component in human milk), into lacto-N-biose I and lactose. Enzymatic release of beta-linked Galbeta1-3GalNAc from natural substrates, these unique activities may play a role in modulating the microbial composition in the gut ecosystem Bifidobacterium longum subsp. Longum

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.336
-
 Gal-beta-(1->3)-GalNAc-beta-(1->3)-Gal-alpha-(1->4)-Gal-beta-(1->4)-Glc pH 6.0, 30°C Bifidobacterium longum subsp. Longum
0.63
-
 Gal-beta-(1->3)-GlcNAc-beta-(1->3)-Gal-beta-(1->4)-Glc pH 6.0, 25°C Bifidobacterium bifidum
0.93
-
 Fuc-alpha-(1->2)-Gal-beta-(1->3)-GlcNAc-beta-(1->3)-Gal-beta-(1->4)-Glc pH 6.0, 25°C Bifidobacterium longum subsp. Longum
39.96
-
 Gal-beta-(1->3)-GalNAc-beta-(1->3)-Gal-alpha-(1->4)-Gal-beta-(1->4)-Glc pH 6.0, 30°C Bifidobacterium bifidum
275
-
 Gal-beta-(1->3)-GlcNAc-beta-(1->3)-Gal-beta-(1->4)-Glc pH 6.0, 25°C Bifidobacterium longum subsp. Longum
300
-
 4-nitrophenyl-Gal-beta-(1->3)-GalNAc pH 6.0, 30°C Bifidobacterium longum subsp. Longum
733.3
-
 4-nitrophenyl-beta-lacto-N-bioside pH 6.0, 30°C Bifidobacterium longum subsp. Longum