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3.2.1.140: lacto-N-biosidase

This is an abbreviated version!
For detailed information about lacto-N-biosidase, go to the full flat file.

Word Map on EC 3.2.1.140

Reaction

beta-D-Gal-(1->3)-beta-D-GlcNAc-(1->3)-beta-D-Gal-(1->4)-D-Glc
+
H2O
=
beta-D-Gal-(1->3)-D-GlcNAc
+
beta-D-Gal-(1->4)-D-Glc

Synonyms

BbLNBase, BLLJ_1505, BLLJ_1506, GH20 lacto-N-biosidase, lacto-N-biosidase, lacto-N-biosidase (Streptomyces strain 142), LNBase, LnbB, LnbX

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.140 lacto-N-biosidase

KCat KM Value

KCat KM Value on EC 3.2.1.140 - lacto-N-biosidase

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kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
733.3
4-nitrophenyl-beta-lacto-N-bioside
pH 6.0, 30°C
300
4-nitrophenyl-Gal-beta-(1->3)-GalNAc
pH 6.0, 30°C
211
4-nitrophenyl-GalNAc-beta-(1->3)-GlcNAc
recombinant enzyme, pH 5.4, 25°C
806
4-nitrophenyl-lacto-N-bioside
recombinant enzyme, pH 5.4, 25°C
0.93
Fuc-alpha-(1->2)-Gal-beta-(1->3)-GlcNAc-beta-(1->3)-Gal-beta-(1->4)-Glc
pH 6.0, 25°C
0.336 - 39.96
Gal-beta-(1->3)-GalNAc-beta-(1->3)-Gal-alpha-(1->4)-Gal-beta-(1->4)-Glc
0.63 - 275
Gal-beta-(1->3)-GlcNAc-beta-(1->3)-Gal-beta-(1->4)-Glc
0.932
lacto-N-fucopentaose I
recombinant enzyme, pH 5.4, 25°C
282
lacto-N-tetraose
recombinant enzyme, pH 5.4, 25°C