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3.2.1.140: lacto-N-biosidase

This is an abbreviated version!
For detailed information about lacto-N-biosidase, go to the full flat file.

Word Map on EC 3.2.1.140

Reaction

beta-D-Gal-(1->3)-beta-D-GlcNAc-(1->3)-beta-D-Gal-(1->4)-D-Glc
+
H2O
=
beta-D-Gal-(1->3)-D-GlcNAc
 +
beta-D-Gal-(1->4)-D-Glc

Synonyms

BbLNBase, BLLJ_1505, BLLJ_1506, GH20 lacto-N-biosidase, lacto-N-biosidase, lacto-N-biosidase (Streptomyces strain 142), LNBase, LnbB, LnbX

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.140 lacto-N-biosidase

KM Value

KM Value on EC 3.2.1.140 - lacto-N-biosidase

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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.12
4-nitrophenyl-beta-lacto-N-bioside
pH 6.0, 30°C
0.005
4-nitrophenyl-Gal-beta-(1->3)-GalNAc
pH 6.0, 30°C
0.186
4-nitrophenyl-GalNAc-beta-(1->3)-GlcNAc
recombinant enzyme, pH 5.4, 25°C
0.119
4-nitrophenyl-lacto-N-bioside
recombinant enzyme, pH 5.4, 25°C
15
Fuc-alpha-(1->2)-Gal-beta-(1->3)-GlcNAc-beta-(1->3)-Gal-beta-(1->4)-Glc
pH 6.0, 25°C
0.0088 - 2.5
Gal-beta-(1->3)-GalNAc-beta-(1->3)-Gal-alpha-(1->4)-Gal-beta-(1->4)-Glc
0.4 - 67
Gal-beta-(1->3)-GlcNAc-beta-(1->3)-Gal-beta-(1->4)-Glc
0.0068
Galbeta1-3GlcNAcbeta1-3Galbeta1-4Glc-pyridylamine
-
-
14.6
lacto-N-fucopentaose I
recombinant enzyme, pH 5.4, 25°C
0.401
lacto-N-tetraose
recombinant enzyme, pH 5.4, 25°C
0.0068
p-nitrophenyl-Gal-(1,3)-beta-GlcNAc
pH 4.5, 25°C
0.068
p-Nitrophenyl-lacto-N-bioside
-
pH 4.5, 25°C
additional information
additional information
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