General Stability | Organism |
---|---|
dilution without Brij58 causes 30-50% inactivation | Streptomyces sp. |
stabilized by 0.05% Brij58, 0.05% Nonidet P-40 or 0.01% bovine serum albumin | Streptomyces sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Fe3+ | - |
Streptomyces sp. | |
guanidine hydrochloride | - |
Streptomyces sp. | |
Hg2+ | - |
Streptomyces sp. | |
lacto-N-biose | - |
Streptomyces sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Streptomyces sp. | |
0.0068 | - |
Galbeta1-3GlcNAcbeta1-3Galbeta1-4Glc-pyridylamine | - |
Streptomyces sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
46000 | - |
gel filtration | Streptomyces sp. |
60000 | - |
1 * 60000, SDS-PAGE | Streptomyces sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces sp. | - |
- |
- |
Streptomyces sp. 142 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Streptomyces sp. |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture medium | - |
Streptomyces sp. | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
18.97 | - |
- |
Streptomyces sp. |
Storage Stability | Organism |
---|---|
4°C or -20°C, protein concentration 0.01 mg/ml or 0.00005 mg/ml, 0.05% Brij 58, stable for at least 6 months | Streptomyces sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Galbeta1-3GlcNAcbeta1-3Galbeta1-4Glc-pyridylamine + H2O | - |
Streptomyces sp. | ? | - |
? | |
Galbeta1-3GlcNAcbeta1-3Galbeta1-4Glc-pyridylamine + H2O | - |
Streptomyces sp. 142 | ? | - |
? | |
p-nitrophenyl-lacto-N-bioside + H2O | - |
Streptomyces sp. | p-nitrophenol + lacto-N-biose | - |
? | |
p-nitrophenyl-lacto-N-bioside + H2O | - |
Streptomyces sp. 142 | p-nitrophenol + lacto-N-biose | - |
? | |
pyridylamine-oligosaccharides which have type 1 chains at the nonreducing terminus + H2O | no reaction with pyridylamine-oligosaccharides which contain only type 2 chains. No reaction with oligosaccharides in which the non-reducing terminal Gal or the penultimate GlcNAc are replaced by fucose or sialic acid are | Streptomyces sp. | lacto-N-biose + ? | - |
? | |
pyridylamine-oligosaccharides which have type 1 chains at the nonreducing terminus + H2O | no reaction with pyridylamine-oligosaccharides which contain only type 2 chains. No reaction with oligosaccharides in which the non-reducing terminal Gal or the penultimate GlcNAc are replaced by fucose or sialic acid are | Streptomyces sp. 142 | lacto-N-biose + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 60000, SDS-PAGE | Streptomyces sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
p-nitrophenyl-beta-lacto-N-bioside | Streptomyces sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
30 min, stable | Streptomyces sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
p-nitrophenyl-beta-lacto-N-bioside | Streptomyces sp. |
5.5 | - |
Galbeta1-3GlcNAcbeta1-3Galbeta1-4Glc-pyridylamine | Streptomyces sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | 10 | 4°C, 16 h, stable | Streptomyces sp. |