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Literature summary for 3.2.1.140 extracted from

  • Sano, M.; Hayakawa, K.; Kato, I.
    Purification and characterization of an enzyme releasing lacto-N-biose from oligosaccharides with type 1 chain (1993), J. Biol. Chem., 268, 18560-18566.
    View publication on PubMed

General Stability

General Stability Organism
dilution without Brij58 causes 30-50% inactivation Streptomyces sp.
stabilized by 0.05% Brij58, 0.05% Nonidet P-40 or 0.01% bovine serum albumin Streptomyces sp.

Inhibitors

Inhibitors Comment Organism Structure
Fe3+
-
Streptomyces sp.
guanidine hydrochloride
-
Streptomyces sp.
Hg2+
-
Streptomyces sp.
lacto-N-biose
-
Streptomyces sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information
-
Streptomyces sp.
0.0068
-
Galbeta1-3GlcNAcbeta1-3Galbeta1-4Glc-pyridylamine
-
Streptomyces sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
46000
-
gel filtration Streptomyces sp.
60000
-
1 * 60000, SDS-PAGE Streptomyces sp.

Organism

Organism UniProt Comment Textmining
Streptomyces sp.
-
-
-
Streptomyces sp. 142
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Streptomyces sp.

Source Tissue

Source Tissue Comment Organism Textmining
culture medium
-
Streptomyces sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
18.97
-
-
Streptomyces sp.

Storage Stability

Storage Stability Organism
4°C or -20°C, protein concentration 0.01 mg/ml or 0.00005 mg/ml, 0.05% Brij 58, stable for at least 6 months Streptomyces sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Galbeta1-3GlcNAcbeta1-3Galbeta1-4Glc-pyridylamine + H2O
-
Streptomyces sp. ?
-
?
Galbeta1-3GlcNAcbeta1-3Galbeta1-4Glc-pyridylamine + H2O
-
Streptomyces sp. 142 ?
-
?
p-nitrophenyl-lacto-N-bioside + H2O
-
Streptomyces sp. p-nitrophenol + lacto-N-biose
-
?
p-nitrophenyl-lacto-N-bioside + H2O
-
Streptomyces sp. 142 p-nitrophenol + lacto-N-biose
-
?
pyridylamine-oligosaccharides which have type 1 chains at the nonreducing terminus + H2O no reaction with pyridylamine-oligosaccharides which contain only type 2 chains. No reaction with oligosaccharides in which the non-reducing terminal Gal or the penultimate GlcNAc are replaced by fucose or sialic acid are Streptomyces sp. lacto-N-biose + ?
-
?
pyridylamine-oligosaccharides which have type 1 chains at the nonreducing terminus + H2O no reaction with pyridylamine-oligosaccharides which contain only type 2 chains. No reaction with oligosaccharides in which the non-reducing terminal Gal or the penultimate GlcNAc are replaced by fucose or sialic acid are Streptomyces sp. 142 lacto-N-biose + ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 60000, SDS-PAGE Streptomyces sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
p-nitrophenyl-beta-lacto-N-bioside Streptomyces sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
30 min, stable Streptomyces sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
p-nitrophenyl-beta-lacto-N-bioside Streptomyces sp.
5.5
-
Galbeta1-3GlcNAcbeta1-3Galbeta1-4Glc-pyridylamine Streptomyces sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 10 4°C, 16 h, stable Streptomyces sp.