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3.2.1.140: lacto-N-biosidase

This is an abbreviated version!
For detailed information about lacto-N-biosidase, go to the full flat file.

Word Map on EC 3.2.1.140

Reaction

beta-D-Gal-(1->3)-beta-D-GlcNAc-(1->3)-beta-D-Gal-(1->4)-D-Glc
+
H2O
=
beta-D-Gal-(1->3)-D-GlcNAc
 +
beta-D-Gal-(1->4)-D-Glc

Synonyms

BbLNBase, BLLJ_1505, BLLJ_1506, GH20 lacto-N-biosidase, lacto-N-biosidase, lacto-N-biosidase (Streptomyces strain 142), LNBase, LnbB, LnbX

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.140 lacto-N-biosidase

Crystallization

Crystallization on EC 3.2.1.140 - lacto-N-biosidase

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
purified enzyme in complex with inhibitors 2-acetamido-2-deoxy-3-O-(beta-D-galactopyranosyl)-D-gluconohydroxyimino-1,5-lactone, 2-acetamido-3-O-(beta-D-galactopyranosyl)-1,5-imino-1,2,5-trideoxy-D-glucitol, 6-acetamido-6-deoxy-7-O-(beta-D-galactopyranosyl)castanospermine, and 8-acetamido-8-deoxy-2-O-(beta-D-galactopyranosyl)australine, sitting drop vapor diffusion method, mixing of 0.001 ml of 10 mg/ml protein solution containing 0.1 mM ligand, with an equal volume of a reservoir solution containing 0.2 M potassium sodium tartrate tetrahydrate, 0.1 M sodium citrate, pH 5.6, and 2.0 M ammonium sulfate, 20°C, X-ray diffraction structure determination and analysis at 1.6-2.2 A resolution, modeling
purified wild-type and selenomethionine-labeled enzymes in complex with Gal-beta-(1->3)-GlcNAc and Gal-beta-(1->3)-GlcNAc-thiazoline, sitting drop vapor diffusion method, mixing of 0.0005 ml of 7 mg/ml protein and 10 mM Gal-beta-(1->3)-GlcNAc with an equal volume of reservoir solution containing 0.2 M potassium sodium tartrate tetrahydrate, 0.1 M sodium citrate, pH 5.6, and 2.0 M ammonium sulfate, X-ray diffraction structure determination and analysis at 1.8 A resolution