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3.2.1.140: lacto-N-biosidase

This is an abbreviated version!
For detailed information about lacto-N-biosidase, go to the full flat file.

Word Map on EC 3.2.1.140

Reaction

beta-D-Gal-(1->3)-beta-D-GlcNAc-(1->3)-beta-D-Gal-(1->4)-D-Glc
+
H2O
=
beta-D-Gal-(1->3)-D-GlcNAc
 +
beta-D-Gal-(1->4)-D-Glc

Synonyms

BbLNBase, BLLJ_1505, BLLJ_1506, GH20 lacto-N-biosidase, lacto-N-biosidase, lacto-N-biosidase (Streptomyces strain 142), LNBase, LnbB, LnbX

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.140 lacto-N-biosidase

Purification

Purification on EC 3.2.1.140 - lacto-N-biosidase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
Ni2+-charged HiTrap chelating column chromatography, followed by superdex 200 10/300 GL column chromatography, purity determined by SDS-PAGE with Coomassie brilliant blue R250 staining
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3)
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) Star by nickel affinity chromatography, gel filtration, and ultrafiltration
recombinant His-tagged wild-type and mutant as well as His-tagged selenomethionine-labeled enzymes from Escherichia coli by nickel affinity and anion exchange chromatography and gel filtration to homogeneity
recombinant non-tagged enzyme 3.6fold from Escherichia coli by two different steps of anion exchange chromataography and hydrophobic interaction chromatography, followed by gel filtration. Recombinant His-tagged enzyme from Escherichia coli by nickel affinity and anion exchange chromatography, and gel filtration
recombinant protein, as the C-terminally His6-tagged protein
-