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0.00012 - 0.00077
5'-phosphoguanylyl(3'->5')guanosine
0.000011 - 6.7
cyclic di-3',5'-guanylate
0.000011 - 0.67
cyclic-di-GMP
0.00012
5'-phosphoguanylyl(3'->5')guanosine
pH 8.0, 30°C, recombinant enzyme
0.0002
5'-phosphoguanylyl(3'->5')guanosine
pH 8.0, 30°C, recombinant wild-type enzyme
0.00077
5'-phosphoguanylyl(3'->5')guanosine
pH 8.0, 30°C, recombinant wild-type enzyme
0.000011
cyclic di-3',5'-guanylate
pH 8.0, 23°C, recombinant mutant E352Q
0.000022
cyclic di-3',5'-guanylate
pH 8.0, 23°C, recombinant mutant E352D
0.00015
cyclic di-3',5'-guanylate
pH 8.0, 30°C, recombinant enzyme
0.0002
cyclic di-3',5'-guanylate
pH 8.0, 30°C, recombinant wild-type enzyme
0.0006
cyclic di-3',5'-guanylate
pH 8.0, 30°C, recombinant mutant enzyme E314A
0.0015
cyclic di-3',5'-guanylate
pH 8.0, 23°C, recombinant mutant E268Q
0.0015
cyclic di-3',5'-guanylate
-
mutant RocR enzyme E268Q, in 100 mM Tris buffer (pH 8.0) (23°C), 20 mM KCl, and 25 mM MgCl2
0.012
cyclic di-3',5'-guanylate
-
mutant RocR enzyme D56N/D296A, in 100 mM Tris buffer (pH 8.0) (23°C), 20 mM KCl, and 25 mM MgCl2
0.02
cyclic di-3',5'-guanylate
-
mutant RocR enzyme F297A, in 100 mM Tris buffer (pH 8.0) (23°C), 20 mM KCl, and 25 mM MgCl2
0.021
cyclic di-3',5'-guanylate
pH 8.0, 23°C, recombinant mutant D296A
0.021
cyclic di-3',5'-guanylate
-
mutant RocR enzyme D296A, in 100 mM Tris buffer (pH 8.0) (23°C), 20 mM KCl, and 25 mM MgCl2
0.023
cyclic di-3',5'-guanylate
pH 8.0, 23°C, recombinant mutant R179A
0.049
cyclic di-3',5'-guanylate
pH 8.0, 23°C, recombinant mutant T267A
0.08
cyclic di-3',5'-guanylate
pH 8.0, 23°C, recombinant mutants Q372A and D318A
0.13
cyclic di-3',5'-guanylate
pH 8.0, 23°C, recombinant mutant Q161A
0.13
cyclic di-3',5'-guanylate
-
mutant RocR enzyme D56N, in 100 mM Tris buffer (pH 8.0) (23°C), 20 mM KCl, and 25 mM MgCl2
0.13
cyclic di-3',5'-guanylate
-
wild type enzyme, at pH 7.5, 20°C, under dark conditions
0.14
cyclic di-3',5'-guanylate
-
mutant RocR enzyme S302A, in 100 mM Tris buffer (pH 8.0) (23°C), 20 mM KCl, and 25 mM MgCl2
0.27
cyclic di-3',5'-guanylate
-
mutant enzyme R93S, at pH 7.5, 20°C, under dark conditions
0.39
cyclic di-3',5'-guanylate
-
wild type PA2567 enzyme, in 100 mM Tris buffer (pH 8.0) (23°C), 50 mM KCl, and 10 mM MgCl2
0.51
cyclic di-3',5'-guanylate
pH 8.0, 23°C, recombinant mutant E355A
0.54
cyclic di-3',5'-guanylate
-
wild type enzyme, at pH 7.5, 20°C, under light conditions
0.64
cyclic di-3',5'-guanylate
-
wild type enzyme, in the presence of 10 mM Mn2+, at pH 7.5, 20°C, under dark conditions
0.67
cyclic di-3',5'-guanylate
pH 8.0, 23°C, recombinant wild-type RocR
0.67
cyclic di-3',5'-guanylate
-
wild type RocR enzyme, in 100 mM Tris buffer (pH 8.0) (23°C), 20 mM KCl, and 25 mM MgCl2
1.1
cyclic di-3',5'-guanylate
-
mutant PA2567 enzyme E464A, in 100 mM Tris buffer (pH 8.0) (23°C), 50 mM KCl, and 10 mM MgCl2
1.17
cyclic di-3',5'-guanylate
-
wild type enzyme, in the presence of 10 mM Mn2+, at pH 7.5, 20°C, under light conditions
1.42
cyclic di-3',5'-guanylate
-
mutant enzyme R93S, at pH 7.5, 20°C, under light conditions
1.84
cyclic di-3',5'-guanylate
-
wild type enzyme, at pH 8.0, 20°C, under dark conditions
2.06
cyclic di-3',5'-guanylate
-
mutant enzyme L128C/G353C, at pH 7.5, 20°C, under dark conditions
2.54
cyclic di-3',5'-guanylate
-
wild type enzyme, at pH 8.0, 20°C, under light conditions
3.48
cyclic di-3',5'-guanylate
-
mutant enzyme L128C/G353C, at pH 7.5, 20°C, under light conditions
6.17
cyclic di-3',5'-guanylate
-
wild type enzyme, at pH 9.3, 20°C, under light conditions
6.7
cyclic di-3',5'-guanylate
-
wild type enzyme, at pH 9.3, 20°C, under dark conditions
0.000011
cyclic-di-GMP
mutant enzyme E352Q, in 100 mM Tris buffer (pH 8.0) with 20 mM KCl and 25 mM MgCl2
0.000022
cyclic-di-GMP
mutant enzyme E352D, in 100 mM Tris buffer (pH 8.0) with 20 mM KCl and 25 mM MgCl2
0.0015
cyclic-di-GMP
mutant enzyme E268Q, in 100 mM Tris buffer (pH 8.0) with 20 mM KCl and 25 mM MgCl2
0.021
cyclic-di-GMP
mutant enzyme D296A, in 100 mM Tris buffer (pH 8.0) with 20 mM KCl and 25 mM MgCl2
0.023
cyclic-di-GMP
mutant enzyme R179A, in 100 mM Tris buffer (pH 8.0) with 20 mM KCl and 25 mM MgCl2
0.049
cyclic-di-GMP
mutant enzyme T267A, in 100 mM Tris buffer (pH 8.0) with 20 mM KCl and 25 mM MgCl2
0.08
cyclic-di-GMP
mutant enzyme D318A, in 100 mM Tris buffer (pH 8.0) with 20 mM KCl and 25 mM MgCl2
0.08
cyclic-di-GMP
mutant enzyme Q372A, in 100 mM Tris buffer (pH 8.0) with 20 mM KCl and 25 mM MgCl2
0.13
cyclic-di-GMP
mutant enzyme Q161A, in 100 mM Tris buffer (pH 8.0) with 20 mM KCl and 25 mM MgCl2
0.51
cyclic-di-GMP
mutant enzyme E355A, in 100 mM Tris buffer (pH 8.0) with 20 mM KCl and 25 mM MgCl2
0.67
cyclic-di-GMP
wild type enzyme, in 100 mM Tris buffer (pH 8.0) with 20 mM KCl and 25 mM MgCl2