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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
crystals are grown by hanging-drop method at 18°C using 0.001 ml of protein solution mixed with an equal volume of reservoir solution, varying conditions using 0.1 M bis-Tris, pH 5.5, 0.2-0.4 M ammonium acetate, 25% w/v PEG 3350, with or without 20% v/v DMSO or 2.1 M DL-malic acid, pH 7.0, X-ray diffraction structure determination and analysis at 2.5 A resolution
purified recombinant detagged enzyme in complex with Fe2+, substrate cyclic di-3',5'-guanylate or final product GMP, hanging drop vapour diffusion method, mixing of equal volumes of 10 mg/ml protein with a precipitant solution made up of 0.1 M MES pH 6.5, 0.9 M succinic acid, and 2% PEG 2000, anomalous X-ray diffraction structure determination and analysis at 2.03-2.8 A resolution