1.1.1.56: ribitol 2-dehydrogenase

This is an abbreviated version, for detailed information about ribitol 2-dehydrogenase, go to the full flat file.

Reaction

ribitol
+
NAD+
=
D-ribulose
+
NADH
+
H+

Synonyms

adonitol dehydrogenase, NAD+-dependent ribitol dehydrogenase, RDH, ribitol dehydrogenase A, ribitol dehydrogenase B, ribitol dehydrogenase D, ribitol-specific dehydrogenase, ribitol: NAD+ 2-oxidoreductase

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.56 ribitol 2-dehydrogenase

Storage Stability

Storage Stability on EC 1.1.1.56 - ribitol 2-dehydrogenase

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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C wild type enzyme shows only slight loss of activity in phosphate buffer pH 7.0
-
2°C presence of NAD+ several days
-
mutant B enzyme inactive after freezing for 2 months, half life of 10 days at 2°C
-
stable at 3°C in phosphate buffer, pH 7.4 for 1 month, unstable below pH 5.5 and at repeated freezing and thawing
-
wild type enzyme can be stored frozen for months without greater loss of activity , half life of 70 days at 2°C
-