Any feedback?
Literature summary for 1.1.1.56 extracted from
- A comparison of wild-type and mutant ribitol dehydrogenases from Klebsiella aerogenes (1974), Biochem. J., 143, 341-352.
General Stability
| General Stability | Organism |
|---|---|
| freezing and thawing causes 30-40% decrease of mutant B enzyme activity | Klebsiella aerogenes |
| freezing and thawing causes 5-8% decrease of wild type enzyme activity | Klebsiella aerogenes |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 8 | - |
ribitol | pH 7 | Klebsiella aerogenes |  |
| 147 | - |
L-arabitol | pH 7 | Klebsiella aerogenes | |
| 579 | - |
xylitol | pH 7 | Klebsiella aerogenes | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 25000 | - |
4 * 25000, wild type enzyme, SDS-PAGE | Klebsiella aerogenes |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ribitol + NAD+ | Klebsiella aerogenes | - |
D-ribulose + NADH | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Klebsiella aerogenes | - |
inducible enzyme | - |
| Klebsiella aerogenes | - |
mutants with constitutive enzyme | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ribitol dehydrogenase B | Klebsiella aerogenes |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 43.6 | - |
ribitol dehydrogenase B | Klebsiella aerogenes |
Storage Stability
| Storage Stability | Organism |
|---|---|
| mutant B enzyme inactive after freezing for 2 months, half life of 10 days at 2°C | Klebsiella aerogenes |
| wild type enzyme can be stored frozen for months without greater loss of activity , half life of 70 days at 2°C | Klebsiella aerogenes |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-arabitol + NAD+ | - |
Klebsiella aerogenes | L-xylulose + NADH + H+ | - |
? | |
| ribitol + NAD+ | - |
Klebsiella aerogenes | D-ribulose + NADH | - |
? | |
| xylitol + NAD+ | mutants have increased xylitol activity | Klebsiella aerogenes | D-xylulose + NADH + H+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | monomers of 27000, mutant B, SDS-PAGE | Klebsiella aerogenes |
| tetramer | 4 * 25000, wild type enzyme, SDS-PAGE | Klebsiella aerogenes |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ribitol dehydrogenase B | mutant enzyme with different properties | Klebsiella aerogenes |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 28 | - |
L-arabitol | - |
Klebsiella aerogenes | |
| 36 | - |
xylitol | - |
Klebsiella aerogenes | |
| 299 | - |
ribitol | - |
Klebsiella aerogenes | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Klebsiella aerogenes | |
| NADH | - |
Klebsiella aerogenes | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
