BRENDA - Enzyme Database show
show all sequences of 1.1.1.56

Ribitol dehydrogenase from Klebsiella aerogenes. Purification and subunit structure

Taylor, S.S.; Rigby, P.W.J.; Hartley, B.S.; Biochem. J. 141, 693-700 (1974)

Data extracted from this reference:

General Stability
General Stability
Organism
NAD+ improves stability and reverses inhibition
Enterobacter aerogenes
unstable in dilute solutions
Enterobacter aerogenes
Inhibitors
Inhibitors
Commentary
Organism
Structure
2-mercaptoethanol
competitive to ribitol
Enterobacter aerogenes
diethyldithiocarbamate
50% inhibition at a concentration of 50 mM
Enterobacter aerogenes
dithiothreitol
-
Enterobacter aerogenes
Hg2+
protection by NAD+
Enterobacter aerogenes
o-phenanthroline
50% inhibition at a concentration of 50 mM
Enterobacter aerogenes
p-chloromercuribenzoate
-
Enterobacter aerogenes
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.1
-
NAD+
pH 11
Enterobacter aerogenes
5
-
ribitol
pH 11
Enterobacter aerogenes
1000
-
xylitol
pH 11
Enterobacter aerogenes
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
additional information
no requirement for metal ions
Enterobacter aerogenes
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
27000
-
4 * 27000, wild type enzyme, SDS-PAGE
Enterobacter aerogenes
100000
110000
wild type, gel filtration
Enterobacter aerogenes
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Enterobacter aerogenes
-
-
-
Purification (Commentary)
Commentary
Organism
wild type
Enterobacter aerogenes
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
83
-
wild type enzyme
Enterobacter aerogenes
Storage Stability
Storage Stability
Organism
-20C wild type enzyme shows only slight loss of activity in phosphate buffer pH 7.0
Enterobacter aerogenes
2C presence of NAD+ several days
Enterobacter aerogenes
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ribitol + NAD+
-
287121
Enterobacter aerogenes
D-ribulose + NADH
-
-
-
-
xylitol + NAD+
-
287121
Enterobacter aerogenes
D-xylulose + NADH + H+
-
-
-
?
Subunits
Subunits
Commentary
Organism
tetramer
4 * 27000, wild type enzyme, SDS-PAGE
Enterobacter aerogenes
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
28
-
-
Enterobacter aerogenes
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
11
-
alcohol oxidation
Enterobacter aerogenes
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
10
11
unstable at high pH values
Enterobacter aerogenes
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
-
Enterobacter aerogenes
NADH
-
Enterobacter aerogenes
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
-
Enterobacter aerogenes
NADH
-
Enterobacter aerogenes
General Stability (protein specific)
General Stability
Organism
NAD+ improves stability and reverses inhibition
Enterobacter aerogenes
unstable in dilute solutions
Enterobacter aerogenes
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
2-mercaptoethanol
competitive to ribitol
Enterobacter aerogenes
diethyldithiocarbamate
50% inhibition at a concentration of 50 mM
Enterobacter aerogenes
dithiothreitol
-
Enterobacter aerogenes
Hg2+
protection by NAD+
Enterobacter aerogenes
o-phenanthroline
50% inhibition at a concentration of 50 mM
Enterobacter aerogenes
p-chloromercuribenzoate
-
Enterobacter aerogenes
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.1
-
NAD+
pH 11
Enterobacter aerogenes
5
-
ribitol
pH 11
Enterobacter aerogenes
1000
-
xylitol
pH 11
Enterobacter aerogenes
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
additional information
no requirement for metal ions
Enterobacter aerogenes
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
27000
-
4 * 27000, wild type enzyme, SDS-PAGE
Enterobacter aerogenes
100000
110000
wild type, gel filtration
Enterobacter aerogenes
Purification (Commentary) (protein specific)
Commentary
Organism
wild type
Enterobacter aerogenes
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
83
-
wild type enzyme
Enterobacter aerogenes
Storage Stability (protein specific)
Storage Stability
Organism
-20C wild type enzyme shows only slight loss of activity in phosphate buffer pH 7.0
Enterobacter aerogenes
2C presence of NAD+ several days
Enterobacter aerogenes
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ribitol + NAD+
-
287121
Enterobacter aerogenes
D-ribulose + NADH
-
-
-
-
xylitol + NAD+
-
287121
Enterobacter aerogenes
D-xylulose + NADH + H+
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
tetramer
4 * 27000, wild type enzyme, SDS-PAGE
Enterobacter aerogenes
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
28
-
-
Enterobacter aerogenes
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
11
-
alcohol oxidation
Enterobacter aerogenes
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
10
11
unstable at high pH values
Enterobacter aerogenes
Other publictions for EC 1.1.1.56
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
737628
Singh
-
D-Ribulose production by a rib ...
Enterobacter aerogenes
Biochem. Eng. J.
109
189-196
2016
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1
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1
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1
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2
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738926
Hassanin
Cloning and characterization o ...
Providencia alcalifaciens, Providencia alcalifaciens RIMD 1656011
J. Sci. Food Agric.
96
2917-2924
2016
-
-
1
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-
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2
2
-
6
1
2
-
2
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-
1
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-
-
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-
6
1
1
1
1
1
1
1
-
1
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-
1
1
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-
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2
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2
-
6
1
2
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1
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-
6
1
1
1
1
1
1
1
-
-
-
-
-
-
1
1
738134
Singh
An efficient ribitol-specific ...
Enterobacter aerogenes, Enterobacter aerogenes KCTC 2190
Enzyme Microb. Technol.
72
56-64
2015
-
-
1
-
-
-
6
2
-
4
1
2
-
5
-
-
1
-
-
-
-
-
4
1
1
1
1
-
1
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-
1
-
-
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-
1
1
-
-
-
-
6
-
2
-
4
1
2
-
-
-
1
-
-
-
-
4
1
1
1
1
-
1
-
-
-
-
-
-
-
1
1
287127
Adachi
Membrane-bound sugar alcohol d ...
Gluconobacter oxydans
Biosci. Biotechnol. Biochem.
65
115-125
2001
-
-
-
1
-
-
-
6
1
-
2
-
-
3
-
-
1
-
-
-
-
-
4
1
-
1
-
-
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2
-
2
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-
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2
1
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6
1
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2
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-
-
1
-
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-
4
1
-
1
-
-
-
2
-
-
-
-
-
-
-
-
287126
Muniruzzaman
-
Purification and characterizat ...
Pantoea agglomerans
J. Ferment. Bioeng.
79
496-498
1995
-
-
-
-
-
-
5
7
-
2
2
-
-
1
-
-
1
-
-
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1
-
7
1
-
-
-
-
1
-
1
1
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-
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1
-
-
-
-
5
-
7
-
2
2
-
-
-
-
1
-
-
1
-
7
1
-
-
-
-
1
-
1
-
-
-
-
-
-
-
287125
Kahle
Pentitol metabolism of Rodobac ...
Rhodobacter sphaeroides
J. Gen. Microbiol.
138
1277-1281
1992
-
-
-
-
-
-
-
5
-
1
2
-
-
4
-
-
1
-
-
-
1
-
5
1
1
-
1
-
2
2
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
5
-
1
2
-
-
-
-
1
-
-
1
-
5
1
1
-
1
-
2
2
-
-
-
-
-
-
-
-
287115
Dothie
Ribitol dehydrogenase of Klebs ...
Enterobacter aerogenes
Biochem. J.
230
569-578
1985
-
-
-
-
1
3
-
-
-
-
-
-
-
1
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1
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2
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1
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1
3
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1
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2
-
-
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1
-
-
-
-
-
-
-
-
-
-
-
287116
Loviny
Ribitol dehydrogenase of Klebs ...
Enterobacter aerogenes
Biochem. J.
230
579-585
1985
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-
1
-
-
-
-
-
-
-
-
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1
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1
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-
287117
Szumiko
The possible occurrence of zin ...
Mycobacterium sp.
Acta Biochim. Pol.
31
401-408
1984
-
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-
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1
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1
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1
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-
287118
Fromm
-
D-Ribulose ...
Enterobacter aerogenes
Methods Enzym. Anal. , 3rd Ed. (Bergmeyer, H. U. , ed. )
6
432-437
1984
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-
-
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1
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1
1
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1
1
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2
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-
287119
Jrnvall
Extended superfamily of short ...
Enterobacter aerogenes
FEBS Lett.
165
190-196
1984
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1
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1
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287120
Burleigh
A comparison of wild-type and ...
Enterobacter aerogenes
Biochem. J.
143
341-352
1974
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2
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3
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1
1
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2
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1
2
3
2
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3
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287121
Taylor
Ribitol dehydrogenase from Kle ...
Enterobacter aerogenes
Biochem. J.
141
693-700
1974
-
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2
6
3
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1
2
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1
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1
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1
2
2
1
1
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1
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1
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2
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2
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6
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3
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1
2
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1
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1
2
2
1
1
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1
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1
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287122
Charnetzky
Ribitol catabolic pathway in K ...
Enterobacter aerogenes
J. Bacteriol.
119
162-169
1974
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287123
Fossitt
-
Pentitol dehydrogenases of Aer ...
Enterobacter aerogenes
Methods Enzymol.
9
180-184
1966
1
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1
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4
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2
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287124
Nordlie
Ribitol dehydrogenase. II. Stu ...
Enterobacter aerogenes
J. Biol. Chem.
234
2523-2531
1959
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