EC Number |
Protein Variants |
Reference |
---|
6.2.1.3 | G344T |
no luminescence enhancement |
697221 |
6.2.1.3 | G344T/L345S |
increase of luminescence activity to 27% |
697221 |
6.2.1.3 | G401L |
inactive enzyme |
672417 |
6.2.1.3 | H316L |
site-directed mutagenesis |
714605 |
6.2.1.3 | K222A |
the ratio of turnover number to Km-value for the reaction with oleate is 8% of the wild-type ratio |
652285 |
6.2.1.3 | K294E |
naturally occuring mutant of dAcs1, K294E is located in the AMP-binding domain |
714799 |
6.2.1.3 | L216R |
no luminescence enhancement |
697221 |
6.2.1.3 | L316H |
site-directed mutagenesis |
714605 |
6.2.1.3 | L345S |
enhancement of luminescence |
697221 |
6.2.1.3 | L399M |
no significant alteration of activity compared to the wild type enzyme |
672417 |