EC Number |
Protein Variants |
Reference |
---|
6.2.1.3 | N431A |
18 site-directed mutantions within the fatty acyl-CoA synthetase structural gene fadD corresponding to the FACS signature. Only one substitution results in wild-type fatty acyl-CoA synthetase activity profiles, 10 substitutions with abolished or greatly diminished enzyme activity, and seven substitutions with altered fatty acid chain length specificity. Of these seven, five also have reduced activity compared with the wild-type enzyme |
739 |
6.2.1.3 | Q419R |
naturally occuring mutant of dAcs1 |
714799 |
6.2.1.3 | Q525K |
reduced activity |
672417 |
6.2.1.3 | S291Y |
mutant prefers 20:5 and 22:6 substrates and has an increased Km for ATP |
672417 |
6.2.1.3 | T214A |
the ratio of turnover number to Km-value for the reaction with oleate is 10.3% of the wild-type ratio |
652285 |
6.2.1.3 | T217A |
the ratio of turnover number to Km-value for the reaction with oleate is 6.7% of the wild-type ratio |
652285 |
6.2.1.3 | W685stop |
naturally occuring mutant of dAcs1, W685stop removes the last 33 amino acids |
714799 |
6.2.1.3 | Y213A |
inactive mutant enzyme |
652285 |
6.2.1.3 | Y319A |
site-directed mutagenesis |
714605 |
6.2.1.3 | Y319F |
site-directed mutagenesis |
714605 |