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Literature summary for 6.2.1.3 extracted from

  • Stinnett, L.; Lewin, T.M.; Coleman, R.A.
    Mutagenesis of rat acyl-CoA synthetase 4 indicates amino acids that contribute to fatty acid binding (2007), Biochim. Biophys. Acta, 1771, 119-125.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.2.1.3

Cloned(Commentary)

Cloned (Comment) Organism
-
 Rattus norvegicus

Protein Variants

Protein Variants Comment Organism
G401L inactive enzyme Rattus norvegicus
L399M no significant alteration of activity compared to the wild type enzyme Rattus norvegicus
Q525K reduced activity Rattus norvegicus
S291Y mutant prefers 20:5 and 22:6 substrates and has an increased Km for ATP Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0064
-
 CoA mutant S291Y, at 37°C, in 175 mM Tris-HCl, at pH 7.4 Rattus norvegicus
0.0067
-
 CoA wild type enzyme, at 37°C, in 175 mM Tris-HCl, at pH 7.4 Rattus norvegicus
0.0068
-
 CoA mutant Q525K, at 37°C, in 175 mM Tris-HCl at pH 7.4 Rattus norvegicus
0.0088
-
 CoA mutant L399M, at 37°C, in 175 mM Tris-HCl at pH 7.4 Rattus norvegicus
0.02
-
 palmitate mutant Q525K, at 37°C, in 175 mM Tris-HCl at pH 7.4 Rattus norvegicus
0.0211
-
 palmitate mutant S291Y, at 37°C, in 175 mM Tris-HCl at pH 7.4 Rattus norvegicus
0.0238
-
 palmitate mutant L399M, at 37°C, in 175 mM Tris-HCl at pH 7.4 Rattus norvegicus
0.0281
-
 palmitate wild type enzyme, at 37°C, in 175 mM Tris-HCl at pH 7.4 Rattus norvegicus
0.087
-
 ATP wild type enzyme, at 37°C, in 175 mM Tris-HCl, at pH 7.4 Rattus norvegicus
0.125
-
 ATP mutant L399M, at 37°C, in 175 mM Tris-HCl at pH 7.4 Rattus norvegicus
0.242
-
 ATP mutant Q525K, at 37°C, in 175 mM Tris-HCl, at pH 7.4 Rattus norvegicus
0.502
-
 ATP mutant S291Y, at 37°C, in 175 mM Tris-HCl, at pH 7.4 Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus O35547
-
-

Purification (Commentary)

Purification (Comment) Organism
M2-anti-FLAG agarose column chromatography Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + arachidonate + CoA the activity with arachidonate is twice as high as with palmitate Rattus norvegicus AMP + diphosphate + arachidonoyl-CoA
-
 ?
ATP + palmitate + CoA
-
 Rattus norvegicus AMP + diphosphate + palmitoyl-CoA
-
 ?

Synonyms

Synonyms Comment Organism
ACSL4 prefers longer chain polyunsaturated fatty acids as substrates Rattus norvegicus
long chain acyl-CoA synthetase 4
-
 Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Rattus norvegicus