6.2.1.3	C343S	no luminescence enhancement	697221	
6.2.1.3	C343S/G344T	no luminescence enhancement	697221	
6.2.1.3	C343S/G344T/L345S	increase of luminescence activity to 100%	697221	
6.2.1.3	C343S/L345S	increase of luminescence activity	697221	
6.2.1.3	E361A	inactive mutant enzyme	652285	
6.2.1.3	F319A	site-directed mutagenesis	714605	
6.2.1.3	F319W	site-directed mutagenesis	714605	
6.2.1.3	F319Y	site-directed mutagenesis	714605	
6.2.1.3	G216A	the ratio of turnover number to Km-value for the reaction with oleate is 32.7% of the wild-type ratio	652285	
6.2.1.3	G219A	the ratio of turnover number to Km-value for the reaction with oleate is 1.3fold higher than the wild-type ratio	652285	
6.2.1.3	G344T	no luminescence enhancement	697221	
6.2.1.3	G344T/L345S	increase of luminescence activity to 27%	697221	
6.2.1.3	G401L	inactive enzyme	672417	
6.2.1.3	H316L	site-directed mutagenesis	714605	
6.2.1.3	K222A	the ratio of turnover number to Km-value for the reaction with oleate is 8% of the wild-type ratio	652285	
6.2.1.3	K294E	naturally occuring mutant of dAcs1, K294E is located in the AMP-binding domain	714799	
6.2.1.3	L216R	no luminescence enhancement	697221	
6.2.1.3	L316H	site-directed mutagenesis	714605	
6.2.1.3	L345S	enhancement of luminescence	697221	
6.2.1.3	L399M	no significant alteration of activity compared to the wild type enzyme	672417	
6.2.1.3	additional information	chimeric protein with firefly luciferase, EC 1.13.12.7, 4% of luminescence activity from Photinus pyralis luciferase activity	697221	
6.2.1.3	additional information	construction of fadD1 mutants, phenotype, overview	716680	
6.2.1.3	additional information	construction of opcl1 mutant, At1g20510-KO-1, plants, which show no induction of jasmonate by wounding in contrast to the wild-type plants, jasmonate profiles in leaves, overview	693420	
6.2.1.3	additional information	generation of fatA, fatB, fatC and fatD deletion strains. Deletion of faaB leads to growth defects on fatty acids but does not affect the induction of genes involved in beta-oxidation, phenotype, overview	713991	
6.2.1.3	additional information	mutants of full-length ACSL6 isoform 2 (F-Gate) and DELTAN-truncated version of isoform 1 (DELTAN-(Y-Gate)) are obtained by site-directed mutagenesis of the H/L residue pair at position 316 and of the F/Y residue pair at position 319. An ACSL6 construct with a tag at its C-terminus has a far lower activity than a construct with a tag fused to its N-terminus	714605	
6.2.1.3	additional information	overexpression of ACSL1 results in a less marked increase in arachidonoyl-CoA synthesis	715838	
6.2.1.3	additional information	overexpression of ACSL5 decreases HepG2 cell viability and increases susceptibility to TRAIL- and TNFalpha-, but not FAS- induced apoptosis, whereas knockdown of ACSL5 reduces apoptosis susceptibility	714313	
6.2.1.3	additional information	recombinant expression of human gene ACSL4 in Drosophila melanogaster dAcsl mutant and complementation of reversed distribution of Rab11	745590	
6.2.1.3	additional information	siRNA knockdown of ACSL5 in rat primary hepatocytes	715835	
6.2.1.3	additional information	specific knockout of ACSL1 in mice leads to 30% increased fat mass when fed a low-fat diet, and normal fat mass with high-fat diet. The mutants incorporate oleate into fatty acids normally, but show 50-90% reduced fatty acid oxidation rates compared to the wild-type. The mutant mice are cold-intolerant and insensitive to beta3-adrenergic agonist activation, phenotype, overview	714678	
6.2.1.3	N431A	18 site-directed mutantions within the fatty acyl-CoA synthetase structural gene fadD corresponding to the FACS signature. Only one substitution results in wild-type fatty acyl-CoA synthetase activity profiles, 10 substitutions with abolished or greatly diminished enzyme activity, and seven substitutions with altered fatty acid chain length specificity. Of these seven, five also have reduced activity compared with the wild-type enzyme	739	
6.2.1.3	Q419R	naturally occuring mutant of dAcs1	714799	
6.2.1.3	Q525K	reduced activity	672417	
6.2.1.3	S291Y	mutant prefers 20:5 and 22:6 substrates and has an increased Km for ATP	672417	
6.2.1.3	T214A	the ratio of turnover number to Km-value for the reaction with oleate is 10.3% of the wild-type ratio	652285	
6.2.1.3	T217A	the ratio of turnover number to Km-value for the reaction with oleate is 6.7% of the wild-type ratio	652285	
6.2.1.3	W685stop	naturally occuring mutant of dAcs1, W685stop removes the last 33 amino acids	714799	
6.2.1.3	Y213A	inactive mutant enzyme	652285	
6.2.1.3	Y319A	site-directed mutagenesis	714605	
6.2.1.3	Y319F	site-directed mutagenesis	714605