EC Number   |
Protein Variants |
Reference |
|---|
   3.4.22.70 | DELTA N59 |
enhanced solubility of the mutant helps the crystallization |
647385 |
| 3.4.22.70 | DELTA N59 |
trucation of N-terminal 59 amino acids does not affect the activity of the enzyme |
647380, 647385 |
| 3.4.22.70 | E108A |
turnover-number for o-aminobenzoyl-LPETG-2,4-dinitrophenyl is 43.8fold lower than wild-type value |
665718 |
| 3.4.22.70 | E171A |
Tm is 1.8°C lower than the Tm-value of wild-type enzyme. kcat/Km is 5.4fold lower than wild-type value |
680902 |
| 3.4.22.70 | E171A |
turnover-number for o-aminobenzoyl-LPETG-2,4-dinitrophenyl is 4.7fold lower than wild-type value |
665718 |
| 3.4.22.70 | G167A |
Tm is 0.5°C lower than the Tm-value of wild-type enzyme. No change in kcat/KM compared to wild-type enzyme |
680902 |
| 3.4.22.70 | H120A |
96000fold decrease in kcat/Km compared to wild-type value |
678296 |
| 3.4.22.70 | H120Q |
36000fold decrease in kcat/Km compared to wild-type value |
678296 |
| 3.4.22.70 | H126A |
inactive |
709123 |
| 3.4.22.70 | I123G |
mutant is generated by using wild type truncated protein SrtADELTAN59 as a template. Mutation reduces dimerization |
678330 |
