Literature summary for 3.4.22.70 extracted from

Naik, M.T.; Suree, N.; Ilangovan, U.; Liew, C.K.; Thieu, W.; Campbell, D.O.; Clemens, J.J.; Jung, M.E.; Clubb, R.T.
Staphylococcus aureus sortase A transpeptidase. Calcium promotes sorting signal binding by altering the mobility and structure of an active site loop (2006), J. Biol. Chem., 281, 1817-1826.

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Protein Variants

Protein Variants	Comment	Organism
D170A	turnover-number for o-aminobenzoyl-LPETG-2,4-dinitrophenyl is nearly identical to wild-type value	Staphylococcus aureus
E108A	turnover-number for o-aminobenzoyl-LPETG-2,4-dinitrophenyl is 43.8fold lower than wild-type value	Staphylococcus aureus
E171A	turnover-number for o-aminobenzoyl-LPETG-2,4-dinitrophenyl is 4.7fold lower than wild-type value	Staphylococcus aureus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.00013	-	o-aminobenzoyl-LPETG-2,4-dinitrophenyl ester	mutant enzyme E108A	Staphylococcus aureus
0.0018	-	o-aminobenzoyl-LPETG-2,4-dinitrophenyl ester	mutant enzyme E171A	Staphylococcus aureus
0.0085	-	o-aminobenzoyl-LPETG-2,4-dinitrophenyl ester	wild-type enzyme	Staphylococcus aureus
0.0087	-	o-aminobenzoyl-LPETG-2,4-dinitrophenyl ester	mutant enzyme D170A	Staphylococcus aureus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Ca2+	a single Ca2+ bound to an ordered pocket on SrtA allosterically activates catalysis by modulating both the structure and dynamics of a large active site loop	Staphylococcus aureus

Organism

Organism	UniProt	Comment	Textmining
Staphylococcus aureus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Staphylococcus aureus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
o-aminobenzoyl-LPETG-2,4-dinitrophenyl ester + H2O	-	Staphylococcus aureus	?	-	?

Synonyms

Synonyms	Comment	Organism
sortase A transpeptidase	-	Staphylococcus aureus

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Release 2023.1 (January 2023)