Protein Variants | Comment | Organism |
---|---|---|
DELTA N59 | trucation of N-terminal 59 amino acids does not affect the activity of the enzyme | Staphylococcus aureus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | class A sortases adopt a type II membrane topology (N terminus inside and C terminus outside the cytoplasm), class B enzymes represent type I membrane proteins (N terminus outside, C terminus inside) | Staphylococcus aureus | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 2 mM, 8fold stimulation of activity, binding near the active site stimulates catalysis possibly by altering the conformation of a surface loop that recognizes newly translocated polypeptides | Staphylococcus aureus | |
Mg2+ | can substitute in part for Ca2+ | Staphylococcus aureus | |
Mn2+ | can substitute in part for Ca2+ | Staphylococcus aureus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
signal peptide, membrane anchor and a shorter linker domain of sortase enzymes display no amino acid conservation. The core residue, SrtA residues 60-206, is present in all sortase homologs examined, suggesting that this domain may comprise the catalytically active domain | Staphylococcus aureus |
16595 | - |
x * 16595, SrtDELTAN59, electrospray ionization mass spectrometry | Staphylococcus aureus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Staphylococcus aureus | the enzyme cleaves surface proteins at the LPXTG motif and catalyzes the formation of an amide bond between the carboxyl group of Thr and the amino group of cell-wall crossbridges | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Staphylococcus aureus | - |
SrtADELTAN, a soluble enzyme lacking the N-terminal signal peptide, residues 2-29 of SrtA and SrtDELTAN59, which contains only the presumed catalytic core | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-[[4'-(dimethylamino)phenyl]azo]-benzoyl-Leu-Pro-Glu-Thr-Gly-5-[(2'-aminoethyl)-amino]naphthalenesulfonic acid + H2O | - |
Staphylococcus aureus | 4-[[4'-(dimethylamino)phenyl]azo]-benzoyl-Leu-Pro-Glu-Thr + Gly-5-[(2'-aminoethyl)-amino]naphthalenesulfonic acid | - |
? | |
additional information | the enzyme cleaves surface proteins at the LPXTG motif and catalyzes the formation of an amide bond between the carboxyl group of Thr and the amino group of cell-wall crossbridges | Staphylococcus aureus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 16595, SrtDELTAN59, electrospray ionization mass spectrometry | Staphylococcus aureus |