Cloned (Comment) | Organism |
---|---|
mutant enzymes expressed in Escherichia coli | Staphylococcus aureus |
Protein Variants | Comment | Organism |
---|---|---|
D170A | Tm is 1.7°C higher than the Tm-value of wild-type enzyme. No change in kcat/Km compared to wild-type value | Staphylococcus aureus |
E171A | Tm is 1.8°C lower than the Tm-value of wild-type enzyme. kcat/Km is 5.4fold lower than wild-type value | Staphylococcus aureus |
G167A | Tm is 0.5°C lower than the Tm-value of wild-type enzyme. No change in kcat/KM compared to wild-type enzyme | Staphylococcus aureus |
L169A | Tm is 1.8°C lower than the Tm-value of wild-type enzyme. kcat/Km is 93fold lower than wild-type value | Staphylococcus aureus |
Q172A | Tm is 1.2°C lower than the Tm-value of wild-type enzyme. kcat/Km is 1.4fold lower than wild-type value | Staphylococcus aureus |
R197A | Tm is 2.9°C lower than the Tm-value of wild-type enzyme. kcat/Km is 960fold lower than wild-type value | Staphylococcus aureus |
R197Cit | generation of a semi-synthetic SrtA in which Arg197 is replaced with citrulline, a nonionizable analog. This change results in less than a 3fold decrease in kcat/KM, indicating that Arg197 utilizes a hydrogen bond, rather than an electrostatic interaction. Tm is 0.3°C higher than the Tm-value of wild-type enzyme | Staphylococcus aureus |
R197Cit | kcat/Km is 5.9fold lower than wild-type value | Staphylococcus aureus |
R197K | Tm is 2°C lower than the Tm-value of wild-type enzyme. kcat/Km is 690fold lower than wild-type value | Staphylococcus aureus |
V168A | Tm is 1.7°C lower than the Tm-value of wild-type enzyme. kcat/Km is 5.5fold lower than wild-type value | Staphylococcus aureus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.69 | - |
aminobenzoyl-LPETGG-diaminopropionic acid(dinitrophenol)-NH2 | 37°C, pH 7.5, mutant enzyme R197A | Staphylococcus aureus | |
6.56 | - |
aminobenzoyl-LPETGG-diaminopropionic acid(dinitrophenol)-NH2 | 37°C, pH 7.5, mutant enzyme V168A | Staphylococcus aureus | |
6.74 | - |
aminobenzoyl-LPETGG-diaminopropionic acid(dinitrophenol)-NH2 | 37°C, pH 7.5, mutant enzyme E171A | Staphylococcus aureus | |
8.13 | - |
aminobenzoyl-LPETGG-diaminopropionic acid(dinitrophenol)-NH2 | 37°C, pH 7.5, mutant enzyme D170A | Staphylococcus aureus | |
8.7 | - |
aminobenzoyl-LPETGG-diaminopropionic acid(dinitrophenol)-NH2 | 37°C, pH 7.5, mutant enzyme G167A | Staphylococcus aureus | |
8.76 | - |
aminobenzoyl-LPETGG-diaminopropionic acid(dinitrophenol)-NH2 | 37°C, pH 7.5, wild-type enzyme | Staphylococcus aureus | |
9.14 | - |
aminobenzoyl-LPETGG-diaminopropionic acid(dinitrophenol)-NH2 | 37°C, pH 7.5, mutant enzyme L169A | Staphylococcus aureus | |
10.4 | - |
aminobenzoyl-LPETGG-diaminopropionic acid(dinitrophenol)-NH2 | 37°C, pH 7.5, mutant enzyme R197K | Staphylococcus aureus | |
12.7 | - |
aminobenzoyl-LPETGG-diaminopropionic acid(dinitrophenol)-NH2 | 37°C, pH 7.5, mutant enzyme Q172A | Staphylococcus aureus | |
13.7 | - |
aminobenzoyl-LPETGG-diaminopropionic acid(dinitrophenol)-NH2 | 37°C, pH 7.5, mutant enzyme R197Cit | Staphylococcus aureus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Staphylococcus aureus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Staphylococcus aureus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Abz-LPETGG-Dap(Dnp)-NH2 + GGGGG | - |
Staphylococcus aureus | Abz-LPETGGGGGG + Gly-Dap(Dnp)-NH2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
SrtA | - |
Staphylococcus aureus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000628 | - |
aminobenzoyl-LPETGG-diaminopropionic acid(dinitrophenol)-NH2 | 37°C, pH 7.5, mutant enzyme R197A | Staphylococcus aureus | |
0.0019 | - |
aminobenzoyl-LPETGG-diaminopropionic acid(dinitrophenol)-NH2 | 37°C, pH 7.5, mutant enzyme R197K | Staphylococcus aureus | |
0.0123 | - |
aminobenzoyl-LPETGG-diaminopropionic acid(dinitrophenol)-NH2 | 37°C, pH 7.5, mutant enzyme L169A | Staphylococcus aureus | |
0.15 | - |
aminobenzoyl-LPETGG-diaminopropionic acid(dinitrophenol)-NH2 | 37°C, pH 7.5, mutant enzyme V168A | Staphylococcus aureus | |
0.16 | - |
aminobenzoyl-LPETGG-diaminopropionic acid(dinitrophenol)-NH2 | 37°C, pH 7.5, mutant enzyme E171A | Staphylococcus aureus | |
0.29 | - |
aminobenzoyl-LPETGG-diaminopropionic acid(dinitrophenol)-NH2 | 37°C, pH 7.5, mutant enzyme R197Cit | Staphylococcus aureus | |
1.09 | - |
aminobenzoyl-LPETGG-diaminopropionic acid(dinitrophenol)-NH2 | 37°C, pH 7.5, mutant enzyme D170A | Staphylococcus aureus | |
1.1 | - |
aminobenzoyl-LPETGG-diaminopropionic acid(dinitrophenol)-NH2 | 37°C, pH 7.5, wild-type enzyme | Staphylococcus aureus | |
1.13 | - |
aminobenzoyl-LPETGG-diaminopropionic acid(dinitrophenol)-NH2 | 37°C, pH 7.5, mutant enzyme G167A | Staphylococcus aureus | |
1.13 | - |
aminobenzoyl-LPETGG-diaminopropionic acid(dinitrophenol)-NH2 | 37°C, pH 7.5, mutant enzyme Q172A | Staphylococcus aureus |