EC Number   |
Protein Variants |
Reference |
|---|
    2.7.1.145 | L349V |
the mutation significantly reduces the enzyme activity |
762135 |
| 2.7.1.145 | M1T/T85A/N121S |
clones harbouring the mutant enzyme are more sensitive to 1-beta-D-arabinofuranosylcytosine and 2',3'-dideoxycytidine |
636844 |
| 2.7.1.145 | M88R |
site-directed mutagenesis of the full length enzyme and of the DELTA20 N-terminally truncated enzyme, highly increased Km for acceptor substrates, altered substrate specificity compared to the wild-type enzyme |
662990 |
| 2.7.1.145 | M88R |
site-directed mutagenesis, altered substrate specificity and kinetics, and highly reduced activity compared to the wild-type enzyme |
661583 |
| 2.7.1.145 | M88R/A110D |
site-directed mutagenesis, altered substrate specificity and kinetics, and highly reduced activity compared to the wild-type enzyme |
661583 |
| 2.7.1.145 | more |
construction of libraries of hybrid enzymes by non-homologous recombination of the pyrimidinespecific human thymidine kinase 2 and the broad-specificity dNK from Drosophila melanogaster. Identification of chimeras that phosphorylate nucleoside analogs with higher activity than either parental enzyme, and that possess new activity towards the anti-HIV prodrug 2',3'-didehydro-3'-deoxythymidine |
675450 |
| 2.7.1.145 | more |
creation of mutants with increased specificity for several nucleoside analogs. The mutants have a reduced ability to phosphorylate pyrimidines, while the ability to phosphorylate purine analogs is relatively similar to the wild-type enzyme |
673848 |
| 2.7.1.145 | more |
engineering of multisubstrate nucleoside kinase targeted to the mitochondrial matrix and expression in thymidine kinase-1 deficient osteosarcoma cell line. Although the total deoxythymidine phosphorylation activity is similar in cells expressing multisubstrate nullceoside kinase in the nucleus or in the mitochondria, the cells expressing the enzyme in the mitochondria show higher sensitivity to the antiproliferative activity of pyrimidine nucleoside analogs, such as (E)-5-(2-bromovinyl)-2-deoxyuridine, 5-bromo-2-deoxyuridine, and 5-fluoro-2-deoxyuridine. Cells expressing the mitochondrial enzyme have an increased incorporation of [3H]dThd into DNA, due to a higher [3H]dTTP specific activity of the total dTTP pool in the cells in which the enzyme is targeted to the mitochondria |
676100 |
| 2.7.1.145 | more |
enzyme DmdNK is adsorbed on a solid ion exchange support (bearing primary amino groups) achieving an expressed activity of over 98%. Upon cross-linking with aldehyde dextran, the expressed activity is 30-40%, both biocatalysts (adsorbed or cross-linked) are stable at pH 10.0 and room temperature for 24 h with about 70% of retained activity. Optimization of the reaction conditions to 50 mM ammonium acetate, a substrate/ATP ratio of 1:1.25, 2 mM MgCl2, 37°C, pH 8.0, results in conversions of up to 90% |
737404 |
| 2.7.1.145 | more |
expression of the enzyme in enzyme-deficient Escherichia coli strain KY895 confers resistnce against 3'-azido-2',3'-didehydro-3'-deoxythymidine at more than 0.1 mM |
661702 |
