BRENDA - Enzyme Database show
show all sequences of 2.7.1.145

A few amino acid substitutions can convert deoxyribonucleoside kinase specificity from pyrimidines to purines

Knecht, W.; Sandrini, M.P.; Johansson, K.; Eklund, H.; Munch-Petersen, B.; Piskur, J.; Embo J. 21, 1873-1880 (2002)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression of wild-type and mutant enzymes in Escherichia coli strain BL21 as C-terminally His8-tagged proteins
Drosophila melanogaster
Engineering
Amino acid exchange
Commentary
Organism
A110D
site-directed mutagenesis, altered substrate specificity and kinetics, and highly reduced activity compared to the wild-type enzyme
Drosophila melanogaster
M88R
site-directed mutagenesis, altered substrate specificity and kinetics, and highly reduced activity compared to the wild-type enzyme
Drosophila melanogaster
M88R/A110D
site-directed mutagenesis, altered substrate specificity and kinetics, and highly reduced activity compared to the wild-type enzyme
Drosophila melanogaster
V84A
site-directed mutagenesis, altered substrate specificity and kinetics, and reduced activity compared to the wild-type enzyme
Drosophila melanogaster
V84A/M88R/A110D
site-directed mutagenesis, altered substrate specificity and kinetics, and highly reduced activity compared to the wild-type enzyme
Drosophila melanogaster
V84M
site-directed mutagenesis, altered substrate specificity and kinetics, and highly reduced activity compared to the wild-type enzyme
Drosophila melanogaster
V84S
site-directed mutagenesis, altered substrate specificity and kinetics, and reduced activity compared to the wild-type enzyme
Drosophila melanogaster
V84S/M88R/A110D
site-directed mutagenesis, altered substrate specificity and kinetics, and highly reduced activity compared to the wild-type enzyme
Drosophila melanogaster
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0012
-
thymidine
recombinant wild-type enzyme
Drosophila melanogaster
0.0023
-
2'-deoxycytidine
recombinant wild-type enzyme
Drosophila melanogaster
0.0025
-
2'-deoxycytidine
recombinant mutant V84A
Drosophila melanogaster
0.0113
-
thymidine
recombinant mutant V84A
Drosophila melanogaster
0.0129
-
2'-deoxycytidine
recombinant mutant V84S
Drosophila melanogaster
0.0247
-
thymidine
recombinant mutant V84S
Drosophila melanogaster
0.04
-
2'-deoxyguanosine
recombinant mutant V84A/M88R/A110D
Drosophila melanogaster
0.064
-
thymidine
recombinant mutant A110D
Drosophila melanogaster
0.091
-
2'-deoxycytidine
recombinant mutant A110D
Drosophila melanogaster
0.147
-
2'-deoxyguanosine
recombinant mutant V84A
Drosophila melanogaster
0.15
-
2'-deoxyadenosine
recombinant mutant V84S
Drosophila melanogaster
0.153
-
2'-deoxyadenosine
recombinant mutant V84A/M88R/A110D
Drosophila melanogaster
0.159
-
2'-deoxyguanosine
recombinant mutant V84S/M88R/A110D
Drosophila melanogaster
0.225
-
2'-deoxyadenosine
recombinant wild-type enzyme
Drosophila melanogaster
0.237
-
thymidine
recombinant mutant M88R
Drosophila melanogaster
0.239
-
2'-deoxyadenosine
recombinant mutant A110D
Drosophila melanogaster
0.257
-
2'-deoxyguanosine
recombinant mutant A110D
Drosophila melanogaster
0.292
-
Dideoxycytidine
recombinant mutant V84A
Drosophila melanogaster
0.36
-
2'-deoxyadenosine
recombinant mutant M88R/A110D
Drosophila melanogaster
0.375
-
2'-deoxyguanosine
recombinant mutant V84S
Drosophila melanogaster
0.388
-
2'-deoxyadenosine
recombinant mutant V84S/M88R/A110D
Drosophila melanogaster
0.549
-
2'-deoxyguanosine
recombinant mutant M88R/A110D
Drosophila melanogaster
0.584
-
2'-deoxycytidine
recombinant mutant M88R/A110D
Drosophila melanogaster
0.627
-
2'-deoxycytidine
recombinant mutant V84A/M88R/A110D
Drosophila melanogaster
0.645
-
2'-deoxyguanosine
recombinant mutant M88R
Drosophila melanogaster
0.665
-
2'-deoxyguanosine
recombinant wild-type enzyme
Drosophila melanogaster
0.674
-
2'-deoxycytidine
recombinant mutant V84M
Drosophila melanogaster
0.7
-
2'-deoxyadenosine
recombinant mutant V84A
Drosophila melanogaster
0.829
-
thymidine
recombinant mutant V84M
Drosophila melanogaster
0.852
-
2'-deoxyadenosine
recombinant mutant V84M
Drosophila melanogaster
1.039
-
2'-deoxycytidine
recombinant mutant M88R
Drosophila melanogaster
1.124
-
Dideoxycytidine
recombinant wild-type enzyme
Drosophila melanogaster
1.512
-
thymidine
recombinant mutant V84A/M88R/A110D
Drosophila melanogaster
1.911
-
2'-deoxyguanosine
recombinant mutant V84M
Drosophila melanogaster
2.088
-
2'-deoxyadenosine
recombinant mutant M88R
Drosophila melanogaster
2.162
-
2'-deoxycytidine
recombinant mutant V84S/M88R/A110D
Drosophila melanogaster
2.425
-
thymidine
recombinant mutant M88R/A110D
Drosophila melanogaster
6.56
-
thymidine
recombinant mutant V84S/M88R/A110D
Drosophila melanogaster
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
-
Drosophila melanogaster
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Drosophila melanogaster
enzyme evolution by gene duplication of a progenitor kinase
?
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Drosophila melanogaster
-
-
-
Purification (Commentary)
Commentary
Organism
recombinant C-terminally His8-tagged wild-type and mutant enzymes from Escherichia coli strain BL21 by nickel affinity chromatography
Drosophila melanogaster
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
catalytic efficiencies of wild-type and mutant enzymes, overview
Drosophila melanogaster
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + 2'-deoxyadenosine
-
661583
Drosophila melanogaster
ADP + 2'-deoxyadenosine 5'-phosphate
-
-
-
?
ATP + 2'-deoxycytidine
wild-type enzyme: second-best substrate
661583
Drosophila melanogaster
ADP + 2'-deoxycytidine 5'-phosphate
-
-
-
?
ATP + 2'-deoxyguanosine
-
661583
Drosophila melanogaster
ADP + 2'-deoxyguanosine 5'-phosphate
-
-
-
?
ATP + dideoxycytidine
wild-type enzyme: low activity
661583
Drosophila melanogaster
ADP + dideoxycytidine 5'-phosphate
-
-
-
?
ATP + thymidine
wild-type enzyme: preferred substrate
661583
Drosophila melanogaster
ADP + thymidine 5'-phosphate
-
-
-
?
additional information
enzyme evolution by gene duplication of a progenitor kinase
661583
Drosophila melanogaster
?
-
-
-
-
additional information
amino acid residues responsible for substrate specificity are V84, M88, and A110, wild-type enzyme shows broad substrate specificity preferring pyrimidines to purines, substrate specificities of wild-type and mutant enzymes, overview
661583
Drosophila melanogaster
?
-
-
-
-
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.034
-
thymidine
recombinant mutant M88R/A110D
Drosophila melanogaster
0.037
-
thymidine
recombinant mutant V84M
Drosophila melanogaster
0.073
-
2'-deoxyguanosine
recombinant mutant A110D
Drosophila melanogaster
0.084
-
2'-deoxyadenosine
recombinant mutant A110D
Drosophila melanogaster
0.11
-
thymidine
recombinant mutant V84A/M88R/A110D
Drosophila melanogaster
0.13
-
thymidine
recombinant mutant M88R
Drosophila melanogaster
0.22
-
2'-deoxyguanosine
recombinant mutant V84M
Drosophila melanogaster
0.23
-
thymidine
recombinant mutant V84S/M88R/A110D
Drosophila melanogaster
0.27
-
2'-deoxycytidine
recombinant mutant A110D
Drosophila melanogaster
0.35
-
thymidine
recombinant mutant A110D
Drosophila melanogaster
0.64
-
2'-deoxyguanosine
recombinant mutant V84S
Drosophila melanogaster
0.83
-
2'-deoxycytidine
recombinant mutant V84S
Drosophila melanogaster
0.83
-
thymidine
recombinant mutant V84S
Drosophila melanogaster
0.93
-
Dideoxycytidine
recombinant mutant V84A
Drosophila melanogaster
1.1
-
2'-deoxyadenosine
recombinant mutant V84S
Drosophila melanogaster
1.36
-
2'-deoxyadenosine
recombinant mutant V84M
Drosophila melanogaster
1.62
-
2'-deoxycytidine
recombinant mutant V84S/M88R/A110D
Drosophila melanogaster
1.73
-
2'-deoxyguanosine
recombinant mutant V84A/M88R/A110D
Drosophila melanogaster
1.8
-
2'-deoxycytidine
recombinant mutant V84M
Drosophila melanogaster
1.88
-
2'-deoxyadenosine
recombinant mutant M88R
Drosophila melanogaster
1.94
-
2'-deoxycytidine
recombinant mutant V84A/M88R/A110D
Drosophila melanogaster
2.7
-
2'-deoxyguanosine
recombinant mutant M88R
Drosophila melanogaster
2.8
-
2'-deoxyguanosine
recombinant mutant V84A
Drosophila melanogaster
2.97
-
thymidine
recombinant mutant V84A
Drosophila melanogaster
3.2
-
2'-deoxyadenosine
recombinant mutant V84S/M88R/A110D
Drosophila melanogaster
3.5
-
2'-deoxyadenosine
recombinant mutant V84A/M88R/A110D
Drosophila melanogaster
3.5
-
2'-deoxycytidine
recombinant mutant M88R/A110D
Drosophila melanogaster
3.7
-
2'-deoxyadenosine
recombinant mutant V84A
Drosophila melanogaster
3.7
-
2'-deoxyguanosine
recombinant mutant M88R/A110D
Drosophila melanogaster
4.2
-
Dideoxycytidine
recombinant wild-type enzyme
Drosophila melanogaster
4.4
-
2'-deoxycytidine
recombinant mutant V84A
Drosophila melanogaster
4.6
-
2'-deoxyguanosine
recombinant mutant V84S/M88R/A110D
Drosophila melanogaster
6.9
-
2'-deoxyadenosine
recombinant mutant M88R/A110D
Drosophila melanogaster
7.5
-
2'-deoxycytidine
recombinant mutant M88R
Drosophila melanogaster
14.2
-
thymidine
recombinant wild-type enzyme
Drosophila melanogaster
15.1
-
2'-deoxyguanosine
recombinant wild-type enzyme
Drosophila melanogaster
16.5
-
2'-deoxycytidine
recombinant wild-type enzyme
Drosophila melanogaster
20.6
-
2'-deoxyadenosine
recombinant wild-type enzyme
Drosophila melanogaster
Cofactor
Cofactor
Commentary
Organism
Structure
ATP
-
Drosophila melanogaster
Cloned(Commentary) (protein specific)
Commentary
Organism
expression of wild-type and mutant enzymes in Escherichia coli strain BL21 as C-terminally His8-tagged proteins
Drosophila melanogaster
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
ATP
-
Drosophila melanogaster
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
A110D
site-directed mutagenesis, altered substrate specificity and kinetics, and highly reduced activity compared to the wild-type enzyme
Drosophila melanogaster
M88R
site-directed mutagenesis, altered substrate specificity and kinetics, and highly reduced activity compared to the wild-type enzyme
Drosophila melanogaster
M88R/A110D
site-directed mutagenesis, altered substrate specificity and kinetics, and highly reduced activity compared to the wild-type enzyme
Drosophila melanogaster
V84A
site-directed mutagenesis, altered substrate specificity and kinetics, and reduced activity compared to the wild-type enzyme
Drosophila melanogaster
V84A/M88R/A110D
site-directed mutagenesis, altered substrate specificity and kinetics, and highly reduced activity compared to the wild-type enzyme
Drosophila melanogaster
V84M
site-directed mutagenesis, altered substrate specificity and kinetics, and highly reduced activity compared to the wild-type enzyme
Drosophila melanogaster
V84S
site-directed mutagenesis, altered substrate specificity and kinetics, and reduced activity compared to the wild-type enzyme
Drosophila melanogaster
V84S/M88R/A110D
site-directed mutagenesis, altered substrate specificity and kinetics, and highly reduced activity compared to the wild-type enzyme
Drosophila melanogaster
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0012
-
thymidine
recombinant wild-type enzyme
Drosophila melanogaster
0.0023
-
2'-deoxycytidine
recombinant wild-type enzyme
Drosophila melanogaster
0.0025
-
2'-deoxycytidine
recombinant mutant V84A
Drosophila melanogaster
0.0113
-
thymidine
recombinant mutant V84A
Drosophila melanogaster
0.0129
-
2'-deoxycytidine
recombinant mutant V84S
Drosophila melanogaster
0.0247
-
thymidine
recombinant mutant V84S
Drosophila melanogaster
0.04
-
2'-deoxyguanosine
recombinant mutant V84A/M88R/A110D
Drosophila melanogaster
0.064
-
thymidine
recombinant mutant A110D
Drosophila melanogaster
0.091
-
2'-deoxycytidine
recombinant mutant A110D
Drosophila melanogaster
0.147
-
2'-deoxyguanosine
recombinant mutant V84A
Drosophila melanogaster
0.15
-
2'-deoxyadenosine
recombinant mutant V84S
Drosophila melanogaster
0.153
-
2'-deoxyadenosine
recombinant mutant V84A/M88R/A110D
Drosophila melanogaster
0.159
-
2'-deoxyguanosine
recombinant mutant V84S/M88R/A110D
Drosophila melanogaster
0.225
-
2'-deoxyadenosine
recombinant wild-type enzyme
Drosophila melanogaster
0.237
-
thymidine
recombinant mutant M88R
Drosophila melanogaster
0.239
-
2'-deoxyadenosine
recombinant mutant A110D
Drosophila melanogaster
0.257
-
2'-deoxyguanosine
recombinant mutant A110D
Drosophila melanogaster
0.292
-
Dideoxycytidine
recombinant mutant V84A
Drosophila melanogaster
0.36
-
2'-deoxyadenosine
recombinant mutant M88R/A110D
Drosophila melanogaster
0.375
-
2'-deoxyguanosine
recombinant mutant V84S
Drosophila melanogaster
0.388
-
2'-deoxyadenosine
recombinant mutant V84S/M88R/A110D
Drosophila melanogaster
0.549
-
2'-deoxyguanosine
recombinant mutant M88R/A110D
Drosophila melanogaster
0.584
-
2'-deoxycytidine
recombinant mutant M88R/A110D
Drosophila melanogaster
0.627
-
2'-deoxycytidine
recombinant mutant V84A/M88R/A110D
Drosophila melanogaster
0.645
-
2'-deoxyguanosine
recombinant mutant M88R
Drosophila melanogaster
0.665
-
2'-deoxyguanosine
recombinant wild-type enzyme
Drosophila melanogaster
0.674
-
2'-deoxycytidine
recombinant mutant V84M
Drosophila melanogaster
0.7
-
2'-deoxyadenosine
recombinant mutant V84A
Drosophila melanogaster
0.829
-
thymidine
recombinant mutant V84M
Drosophila melanogaster
0.852
-
2'-deoxyadenosine
recombinant mutant V84M
Drosophila melanogaster
1.039
-
2'-deoxycytidine
recombinant mutant M88R
Drosophila melanogaster
1.124
-
Dideoxycytidine
recombinant wild-type enzyme
Drosophila melanogaster
1.512
-
thymidine
recombinant mutant V84A/M88R/A110D
Drosophila melanogaster
1.911
-
2'-deoxyguanosine
recombinant mutant V84M
Drosophila melanogaster
2.088
-
2'-deoxyadenosine
recombinant mutant M88R
Drosophila melanogaster
2.162
-
2'-deoxycytidine
recombinant mutant V84S/M88R/A110D
Drosophila melanogaster
2.425
-
thymidine
recombinant mutant M88R/A110D
Drosophila melanogaster
6.56
-
thymidine
recombinant mutant V84S/M88R/A110D
Drosophila melanogaster
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
-
Drosophila melanogaster
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Drosophila melanogaster
enzyme evolution by gene duplication of a progenitor kinase
?
-
-
-
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant C-terminally His8-tagged wild-type and mutant enzymes from Escherichia coli strain BL21 by nickel affinity chromatography
Drosophila melanogaster
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
catalytic efficiencies of wild-type and mutant enzymes, overview
Drosophila melanogaster
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + 2'-deoxyadenosine
-
661583
Drosophila melanogaster
ADP + 2'-deoxyadenosine 5'-phosphate
-
-
-
?
ATP + 2'-deoxycytidine
wild-type enzyme: second-best substrate
661583
Drosophila melanogaster
ADP + 2'-deoxycytidine 5'-phosphate
-
-
-
?
ATP + 2'-deoxyguanosine
-
661583
Drosophila melanogaster
ADP + 2'-deoxyguanosine 5'-phosphate
-
-
-
?
ATP + dideoxycytidine
wild-type enzyme: low activity
661583
Drosophila melanogaster
ADP + dideoxycytidine 5'-phosphate
-
-
-
?
ATP + thymidine
wild-type enzyme: preferred substrate
661583
Drosophila melanogaster
ADP + thymidine 5'-phosphate
-
-
-
?
additional information
enzyme evolution by gene duplication of a progenitor kinase
661583
Drosophila melanogaster
?
-
-
-
-
additional information
amino acid residues responsible for substrate specificity are V84, M88, and A110, wild-type enzyme shows broad substrate specificity preferring pyrimidines to purines, substrate specificities of wild-type and mutant enzymes, overview
661583
Drosophila melanogaster
?
-
-
-
-
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.034
-
thymidine
recombinant mutant M88R/A110D
Drosophila melanogaster
0.037
-
thymidine
recombinant mutant V84M
Drosophila melanogaster
0.073
-
2'-deoxyguanosine
recombinant mutant A110D
Drosophila melanogaster
0.084
-
2'-deoxyadenosine
recombinant mutant A110D
Drosophila melanogaster
0.11
-
thymidine
recombinant mutant V84A/M88R/A110D
Drosophila melanogaster
0.13
-
thymidine
recombinant mutant M88R
Drosophila melanogaster
0.22
-
2'-deoxyguanosine
recombinant mutant V84M
Drosophila melanogaster
0.23
-
thymidine
recombinant mutant V84S/M88R/A110D
Drosophila melanogaster
0.27
-
2'-deoxycytidine
recombinant mutant A110D
Drosophila melanogaster
0.35
-
thymidine
recombinant mutant A110D
Drosophila melanogaster
0.64
-
2'-deoxyguanosine
recombinant mutant V84S
Drosophila melanogaster
0.83
-
2'-deoxycytidine
recombinant mutant V84S
Drosophila melanogaster
0.83
-
thymidine
recombinant mutant V84S
Drosophila melanogaster
0.93
-
Dideoxycytidine
recombinant mutant V84A
Drosophila melanogaster
1.1
-
2'-deoxyadenosine
recombinant mutant V84S
Drosophila melanogaster
1.36
-
2'-deoxyadenosine
recombinant mutant V84M
Drosophila melanogaster
1.62
-
2'-deoxycytidine
recombinant mutant V84S/M88R/A110D
Drosophila melanogaster
1.73
-
2'-deoxyguanosine
recombinant mutant V84A/M88R/A110D
Drosophila melanogaster
1.8
-
2'-deoxycytidine
recombinant mutant V84M
Drosophila melanogaster
1.88
-
2'-deoxyadenosine
recombinant mutant M88R
Drosophila melanogaster
1.94
-
2'-deoxycytidine
recombinant mutant V84A/M88R/A110D
Drosophila melanogaster
2.7
-
2'-deoxyguanosine
recombinant mutant M88R
Drosophila melanogaster
2.8
-
2'-deoxyguanosine
recombinant mutant V84A
Drosophila melanogaster
2.97
-
thymidine
recombinant mutant V84A
Drosophila melanogaster
3.2
-
2'-deoxyadenosine
recombinant mutant V84S/M88R/A110D
Drosophila melanogaster
3.5
-
2'-deoxyadenosine
recombinant mutant V84A/M88R/A110D
Drosophila melanogaster
3.5
-
2'-deoxycytidine
recombinant mutant M88R/A110D
Drosophila melanogaster
3.7
-
2'-deoxyadenosine
recombinant mutant V84A
Drosophila melanogaster
3.7
-
2'-deoxyguanosine
recombinant mutant M88R/A110D
Drosophila melanogaster
4.2
-
Dideoxycytidine
recombinant wild-type enzyme
Drosophila melanogaster
4.4
-
2'-deoxycytidine
recombinant mutant V84A
Drosophila melanogaster
4.6
-
2'-deoxyguanosine
recombinant mutant V84S/M88R/A110D
Drosophila melanogaster
6.9
-
2'-deoxyadenosine
recombinant mutant M88R/A110D
Drosophila melanogaster
7.5
-
2'-deoxycytidine
recombinant mutant M88R
Drosophila melanogaster
14.2
-
thymidine
recombinant wild-type enzyme
Drosophila melanogaster
15.1
-
2'-deoxyguanosine
recombinant wild-type enzyme
Drosophila melanogaster
16.5
-
2'-deoxycytidine
recombinant wild-type enzyme
Drosophila melanogaster
20.6
-
2'-deoxyadenosine
recombinant wild-type enzyme
Drosophila melanogaster
Other publictions for EC 2.7.1.145
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
738739
Kamiya
In vivo selection of active de ...
Drosophila melanogaster
J. Biotechnol.
228
52-57
2016
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738781
Slot Christiansen
Non-viral deoxyribonucleoside ...
Anopheles gambiae, Bombyx mori, Drosophila melanogaster
J. Genet. Genomics
42
235-248
2015
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7
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739211
Jiang
Tanshinone IIA enhances bystan ...
Drosophila melanogaster
Oncol. Rep.
34
1487-1493
2015
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737404
Serra
-
Immobilized Drosophila melanog ...
Drosophila melanogaster
Adv. Synth. Catal.
356
563-570
2014
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703396
Betham
Use of Drosophila deoxynucleos ...
Drosophila melanogaster
DNA Repair
9
153-160
2010
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721775
Ito
Cytotoxic effect of Drosophila ...
Drosophila melanogaster
Biol. Pharm. Bull.
33
1223-1227
2010
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702047
Knecht
Drosophila melanogaster deoxyr ...
Drosophila melanogaster
Biochem. Biophys. Res. Commun.
382
430-433
2009
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692289
Mikkelsen
Structural studies of nucleosi ...
Drosophila melanogaster
FEBS J.
275
2151-2160
2008
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694476
Clausen
A multisubstrate deoxyribonucl ...
Arabidopsis thaliana
Nucleic Acids Symp. Ser.
52
489-490
2008
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673584
Egeblad-Welin
Functional studies of active-s ...
Drosophila melanogaster
FEBS J.
274
1542-1551
2007
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673848
Knecht
Drosophila deoxyribonucleoside ...
Drosophila melanogaster
Gene Ther.
14
1278-1286
2007
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675450
Gerth
Non-homologous recombination o ...
Drosophila melanogaster
J. Mol. Biol.
370
742-751
2007
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6
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676100
Solaroli
Mitochondrial expression of th ...
Drosophila melanogaster
Mol. Pharmacol.
72
1593-1598
2007
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672880
Jordheim
Gemcitabine resistance due to ...
Drosophila melanogaster
Cancer Chemother. Pharmacol.
58
547-554
2006
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672971
Legent
In vivo analysis of Drosophila ...
Drosophila melanogaster
Cell Cycle
5
740-749
2006
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676247
Kamiya
Transient expression of Drosop ...
Drosophila melanogaster
Nucleosides Nucleotides Nucleic Acids
25
553-560
2006
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661702
Welin
Structural basis for the chang ...
Drosophila melanogaster
FEBS J.
272
3733-3742
2005
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10
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9
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10
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7
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9
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661731
Piskur
Animal deoxyribonucleoside kin ...
Drosophila melanogaster
FEBS Lett.
560
3-6
2004
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662990
Solaroli
Investigation of the substrate ...
Drosophila melanogaster
Nucleosides Nucleotides Nucleic Acids
23
1527-1529
2004
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10
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1
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660855
Krawiec
Inorganic tripolyphosphate (PP ...
Drosophila melanogaster
Biochem. Biophys. Res. Commun.
301
192-197
2003
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661072
Mikkelsen
Structural basis for feedback ...
Drosophila melanogaster
Biochemistry
42
5706-5712
2003
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661629
Solaroli
Active site mutants of Drosoph ...
Drosophila melanogaster
Eur. J. Biochem.
270
2879-2884
2003
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1
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9
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9
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3
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9
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9
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662967
Knecht
Mosquito has a single multisub ...
Anopheles gambiae
Nucleic Acids Res.
31
1665-1672
2003
-
2
1
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4
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1
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3
1
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14
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1
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1
1
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4
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3
1
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14
-
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4
1
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662985
Krawiec
Ability of adenosine-2'(3')-de ...
Drosophila melanogaster
Nucleosides Nucleotides Nucleic Acids
22
1525-1529
2003
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661453
Eriksson
Structure and function of cell ...
Bombyx mori, Drosophila melanogaster
Cell. Mol. Life Sci.
59
1327-1346
2002
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4
1
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1
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2
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2
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2
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2
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11
4
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1
1
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11
4
-
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661583
Knecht
A few amino acid substitutions ...
Drosophila melanogaster
Embo J.
21
1873-1880
2002
-
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1
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8
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38
-
1
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6
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1
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1
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7
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38
-
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1
1
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8
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38
-
1
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1
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1
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7
-
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38
-
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662607
Knecht
Deoxyribonucleoside kinases be ...
Bombyx mori, Drosophila melanogaster, Xenopus laevis
J. Mol. Biol.
315
529-540
2002
-
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3
-
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6
12
2
2
12
2
-
8
-
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3
-
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1
2
-
39
5
-
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2
3
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3
2
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6
3
12
2
2
12
2
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3
-
1
2
-
39
5
-
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660852
Zheng
Nucleoside analog cytotoxicity ...
Drosophila melanogaster
Biochem. Biophys. Res. Commun.
289
229-233
2001
-
1
1
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2
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1
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3
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1
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662932
Johansson
Structural basis for substrate ...
Drosophila melanogaster
Nat. Struct. Biol.
8
616-620
2001
-
1
-
1
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1
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1
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3
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1
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2
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636842
Munch-Petersen
Functional expression of a mul ...
Drosophila melanogaster
J. Biol. Chem.
275
6673-6679
2000
-
-
1
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1
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4
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1
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5
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1
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4
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18
1
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4
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1
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1
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4
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1
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1
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4
-
18
1
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4
-
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636843
Balzarini
The multifunctional deoxynucle ...
Drosophila melanogaster
Mol. Pharmacol.
57
811-819
2000
-
1
1
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5
1
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1
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4
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3
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1
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3
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1
1
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3
5
1
1
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1
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3
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17
-
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12
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11
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1
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8
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17
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4
1
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15
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1
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15
17
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4
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1
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4
1
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1
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1
1
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15
1
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1
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3
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4
3
4
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2
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1
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1
1
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15
1
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1
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21
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1
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4
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8
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4
8
5
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2
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1
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1
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21
1
1
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4
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