BRENDA - Enzyme Database show
show all sequences of 2.7.1.145

Immobilized Drosophila melanogaster deoxyribonucleoside kinase (DmdNK) as a high performing biocatalyst for the synthesis of purine arabinonucleotides

Serra, I.; Conti, S.; Piškur, J.; Clausen, A.; Munch-Petersen, B.; Terreni, M.; Ubiali, D.; Adv. Synth. Catal. 356, 563-570 (2014)
No PubMed abstract available

Data extracted from this reference:

Application
Application
Commentary
Organism
synthesis
the cross-linked DmdNK preparation is used for the preparative synthesis of arabinosyladenine monophosphate (ara-AMP) and fludarabine monophosphate (FaraAMP)
Drosophila melanogaster
Engineering
Amino acid exchange
Commentary
Organism
additional information
enzyme DmdNK is adsorbed on a solid ion exchange support (bearing primary amino groups) achieving an expressed activity of over 98%. Upon cross-linking with aldehyde dextran, the expressed activity is 30-40%, both biocatalysts (adsorbed or cross-linked) are stable at pH 10.0 and room temperature for 24 h with about 70% of retained activity. Optimization of the reaction conditions to 50 mM ammonium acetate, a substrate/ATP ratio of 1:1.25, 2 mM MgCl2, 37°C, pH 8.0, results in conversions of up to 90%
Drosophila melanogaster
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
required
Drosophila melanogaster
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ATP + 2'-deoxyribonucleoside
Drosophila melanogaster
-
ADP + 2'-deoxyribonucleoside 5'-phosphate
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Drosophila melanogaster
Q9XZT6
gene dnk
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + 2'-deoxycytidine
-
737404
Drosophila melanogaster
ADP + 2'-deoxycytidine 5'-phosphate
-
-
-
?
ATP + 2'-deoxyguanosine
-
737404
Drosophila melanogaster
ADP + 2'-deoxyguanosine 5'-phosphate
-
-
-
?
ATP + 2'-deoxyinosine
-
737404
Drosophila melanogaster
ADP + 2'-deoxyinosine 5'-phosphate
-
-
-
?
ATP + 2'-deoxyribonucleoside
-
737404
Drosophila melanogaster
ADP + 2'-deoxyribonucleoside 5'-phosphate
-
-
-
?
ATP + adenosine
low activity
737404
Drosophila melanogaster
ADP + adenosine 5'-phosphate
-
-
-
?
ATP + arabinosyl-2-fluoroadenine
-
737404
Drosophila melanogaster
ADP + arabinosyl-2-fluoroadenine 5'-monophosphate
-
-
-
?
ATP + arabinosyladenine
-
737404
Drosophila melanogaster
ADP + arabinosyladenine 5'-monophosphate
-
-
-
?
ATP + arabinosyluridine
-
737404
Drosophila melanogaster
ADP + arabinosyluridine 5'-monophosphate
-
-
-
?
ATP + cytidine
-
737404
Drosophila melanogaster
ADP + cytidine 5'-phosphate
-
-
-
?
ATP + guanosine
low activity
737404
Drosophila melanogaster
ADP + guanosine 5'-phosphate
-
-
-
?
ATP + inosine
very low activity
737404
Drosophila melanogaster
ADP + inosine 5'-phosphate
-
-
-
?
ATP + thymidine
-
737404
Drosophila melanogaster
ADP + thymidine 5'-phosphate
-
-
-
?
ATP + uridine
very low activity
737404
Drosophila melanogaster
ADP + uridine 5'-phosphate
-
-
-
?
additional information
enzyme DmdNK is able to phosphorylate all four natural 2'-deoxyribonucleosides with high efficiency, it shows broad specificity and high turnover towards the naturally occurring 2'-deoxyribonucleosides and a similar broad specificity towards nucleoside analogues used in chemotherapy, substrate specificity towards natural and non-natural nucleosides, overview
737404
Drosophila melanogaster
?
-
-
-
-
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
immobilized enzyme
Drosophila melanogaster
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
immobilized enzyme
Drosophila melanogaster
Cofactor
Cofactor
Commentary
Organism
Structure
ATP
-
Drosophila melanogaster
Application (protein specific)
Application
Commentary
Organism
synthesis
the cross-linked DmdNK preparation is used for the preparative synthesis of arabinosyladenine monophosphate (ara-AMP) and fludarabine monophosphate (FaraAMP)
Drosophila melanogaster
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
ATP
-
Drosophila melanogaster
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
additional information
enzyme DmdNK is adsorbed on a solid ion exchange support (bearing primary amino groups) achieving an expressed activity of over 98%. Upon cross-linking with aldehyde dextran, the expressed activity is 30-40%, both biocatalysts (adsorbed or cross-linked) are stable at pH 10.0 and room temperature for 24 h with about 70% of retained activity. Optimization of the reaction conditions to 50 mM ammonium acetate, a substrate/ATP ratio of 1:1.25, 2 mM MgCl2, 37°C, pH 8.0, results in conversions of up to 90%
Drosophila melanogaster
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
required
Drosophila melanogaster
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ATP + 2'-deoxyribonucleoside
Drosophila melanogaster
-
ADP + 2'-deoxyribonucleoside 5'-phosphate
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + 2'-deoxycytidine
-
737404
Drosophila melanogaster
ADP + 2'-deoxycytidine 5'-phosphate
-
-
-
?
ATP + 2'-deoxyguanosine
-
737404
Drosophila melanogaster
ADP + 2'-deoxyguanosine 5'-phosphate
-
-
-
?
ATP + 2'-deoxyinosine
-
737404
Drosophila melanogaster
ADP + 2'-deoxyinosine 5'-phosphate
-
-
-
?
ATP + 2'-deoxyribonucleoside
-
737404
Drosophila melanogaster
ADP + 2'-deoxyribonucleoside 5'-phosphate
-
-
-
?
ATP + adenosine
low activity
737404
Drosophila melanogaster
ADP + adenosine 5'-phosphate
-
-
-
?
ATP + arabinosyl-2-fluoroadenine
-
737404
Drosophila melanogaster
ADP + arabinosyl-2-fluoroadenine 5'-monophosphate
-
-
-
?
ATP + arabinosyladenine
-
737404
Drosophila melanogaster
ADP + arabinosyladenine 5'-monophosphate
-
-
-
?
ATP + arabinosyluridine
-
737404
Drosophila melanogaster
ADP + arabinosyluridine 5'-monophosphate
-
-
-
?
ATP + cytidine
-
737404
Drosophila melanogaster
ADP + cytidine 5'-phosphate
-
-
-
?
ATP + guanosine
low activity
737404
Drosophila melanogaster
ADP + guanosine 5'-phosphate
-
-
-
?
ATP + inosine
very low activity
737404
Drosophila melanogaster
ADP + inosine 5'-phosphate
-
-
-
?
ATP + thymidine
-
737404
Drosophila melanogaster
ADP + thymidine 5'-phosphate
-
-
-
?
ATP + uridine
very low activity
737404
Drosophila melanogaster
ADP + uridine 5'-phosphate
-
-
-
?
additional information
enzyme DmdNK is able to phosphorylate all four natural 2'-deoxyribonucleosides with high efficiency, it shows broad specificity and high turnover towards the naturally occurring 2'-deoxyribonucleosides and a similar broad specificity towards nucleoside analogues used in chemotherapy, substrate specificity towards natural and non-natural nucleosides, overview
737404
Drosophila melanogaster
?
-
-
-
-
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
immobilized enzyme
Drosophila melanogaster
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
immobilized enzyme
Drosophila melanogaster
Other publictions for EC 2.7.1.145
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
738739
Kamiya
In vivo selection of active de ...
Drosophila melanogaster
J. Biotechnol.
228
52-57
2016
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1
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1
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1
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1
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1
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3
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1
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1
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1
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1
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1
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3
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1
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1
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1
1
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-
738781
Slot Christiansen
Non-viral deoxyribonucleoside ...
Anopheles gambiae, Bombyx mori, Drosophila melanogaster
J. Genet. Genomics
42
235-248
2015
-
2
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1
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1
3
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18
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3
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33
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3
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2
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3
1
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1
3
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18
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33
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7
7
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739211
Jiang
Tanshinone IIA enhances bystan ...
Drosophila melanogaster
Oncol. Rep.
34
1487-1493
2015
-
1
1
-
-
-
-
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4
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1
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3
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1
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3
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1
1
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737404
Serra
-
Immobilized Drosophila melanog ...
Drosophila melanogaster
Adv. Synth. Catal.
356
563-570
2014
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1
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1
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1
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1
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1
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14
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14
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1
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1
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703396
Betham
Use of Drosophila deoxynucleos ...
Drosophila melanogaster
DNA Repair
9
153-160
2010
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1
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4
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1
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1
1
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721775
Ito
Cytotoxic effect of Drosophila ...
Drosophila melanogaster
Biol. Pharm. Bull.
33
1223-1227
2010
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1
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5
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702047
Knecht
Drosophila melanogaster deoxyr ...
Drosophila melanogaster
Biochem. Biophys. Res. Commun.
382
430-433
2009
-
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1
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1
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3
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1
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1
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1
1
692289
Mikkelsen
Structural studies of nucleosi ...
Drosophila melanogaster
FEBS J.
275
2151-2160
2008
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1
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2
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694476
Clausen
A multisubstrate deoxyribonucl ...
Arabidopsis thaliana
Nucleic Acids Symp. Ser.
52
489-490
2008
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1
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2
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7
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7
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673584
Egeblad-Welin
Functional studies of active-s ...
Drosophila melanogaster
FEBS J.
274
1542-1551
2007
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1
7
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18
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18
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18
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5
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18
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673848
Knecht
Drosophila deoxyribonucleoside ...
Drosophila melanogaster
Gene Ther.
14
1278-1286
2007
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1
1
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2
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4
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675450
Gerth
Non-homologous recombination o ...
Drosophila melanogaster
J. Mol. Biol.
370
742-751
2007
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6
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1
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676100
Solaroli
Mitochondrial expression of th ...
Drosophila melanogaster
Mol. Pharmacol.
72
1593-1598
2007
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1
1
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1
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2
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672880
Jordheim
Gemcitabine resistance due to ...
Drosophila melanogaster
Cancer Chemother. Pharmacol.
58
547-554
2006
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672971
Legent
In vivo analysis of Drosophila ...
Drosophila melanogaster
Cell Cycle
5
740-749
2006
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676247
Kamiya
Transient expression of Drosop ...
Drosophila melanogaster
Nucleosides Nucleotides Nucleic Acids
25
553-560
2006
-
1
1
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661702
Welin
Structural basis for the chang ...
Drosophila melanogaster
FEBS J.
272
3733-3742
2005
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1
1
4
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1
10
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1
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4
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2
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1
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7
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9
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4
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1
1
4
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1
4
10
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1
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1
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7
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9
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661731
Piskur
Animal deoxyribonucleoside kin ...
Drosophila melanogaster
FEBS Lett.
560
3-6
2004
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662990
Solaroli
Investigation of the substrate ...
Drosophila melanogaster
Nucleosides Nucleotides Nucleic Acids
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660855
Krawiec
Inorganic tripolyphosphate (PP ...
Drosophila melanogaster
Biochem. Biophys. Res. Commun.
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2003
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Mikkelsen
Structural basis for feedback ...
Drosophila melanogaster
Biochemistry
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2003
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661629
Solaroli
Active site mutants of Drosoph ...
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2003
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9
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662967
Knecht
Mosquito has a single multisub ...
Anopheles gambiae
Nucleic Acids Res.
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2003
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2
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14
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1
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14
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1
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662985
Krawiec
Ability of adenosine-2'(3')-de ...
Drosophila melanogaster
Nucleosides Nucleotides Nucleic Acids
22
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2003
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661453
Eriksson
Structure and function of cell ...
Bombyx mori, Drosophila melanogaster
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2002
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4
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661583
Knecht
A few amino acid substitutions ...
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38
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8
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38
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7
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38
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Knecht
Deoxyribonucleoside kinases be ...
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2002
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3
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3
2
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6
3
12
2
2
12
2
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1
2
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39
5
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660852
Zheng
Nucleoside analog cytotoxicity ...
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2001
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662932
Johansson
Structural basis for substrate ...
Drosophila melanogaster
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636842
Munch-Petersen
Functional expression of a mul ...
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1
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1
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636843
Balzarini
The multifunctional deoxynucle ...
Drosophila melanogaster
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Knecht
Identification of residues inv ...
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12
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17
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636841
Johansson
Cloning and characterization o ...
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17
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636839
Munch-Petersen
The single deoxynucleoside kin ...
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636840
Munch-Petersen
Four deoxynucleoside kinase ac ...
Drosophila melanogaster, Drosophila melanogaster S-2
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8
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21
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