EC Number |
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Reference |
---|
4.3.1.7 | -999 |
- |
activity of the purified enzyme with aminoethanol as substrate is 20-30 micromol/min/mg. |
692739 |
4.3.1.7 | -999 |
- |
enzyme activity is determined by using the coupled assay with alcohol dehydrogenase/NADH, the specific activity of the purified enzyme with aminoethanol as substrate was 20-30 micromol/min/mg |
691337 |
4.3.1.7 | -999 |
- |
Externally added guanidine hydrochloride leads to significant R160A EAL enzyme activity under Vmax conditions, the activity of R160A EAL increases with guanidine hydrochloride concentration and reaches a maximum at 10 mM, which represents 2.3% of the activity of the wild-type control. The resurrection of enzyme activity in R160A EAL by externally added guanidine hydrochloride provides strong support that a positively charged arginine side chain in the active site is catalytically essential. |
690960 |
4.3.1.7 | -999 |
- |
specific activity is 30-45 micromol ethanolamine/min/mg of EAL at 25°C, determined by the EAL-alcohol dehydrogenase coupled assay |
690905 |
4.3.1.7 | -999 |
- |
specific activity of the purified enzyme with aminoethanol as the substrate is 35-45 micromol/min/mg, determined by using the coupled assay with alcohol dehydrogenase/NADH |
691009 |
4.3.1.7 | -999 |
- |
truncated enzymes, after purificaton, value between 12.45 and 63.1 |
704362 |
4.3.1.7 | 13.9 |
- |
- |
34382 |
4.3.1.7 | 14.1 |
- |
- |
34370, 34375 |
4.3.1.7 | 25.3 |
- |
- |
34384 |
4.3.1.7 | 35 |
- |
- |
34380 |