Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli | Salmonella enterica subsp. enterica serovar Typhimurium |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
hydroxyethylhydrazine | the latter suicide inhibitor effects a stoichiometric conversion of enzyme-bound adenosylcobalamin into its cleaved form cob(II)alamin | Salmonella enterica subsp. enterica serovar Typhimurium |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | included in the cofactor B12 | Salmonella enterica subsp. enterica serovar Typhimurium |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
31000 | - |
beta-subunit | Salmonella enterica subsp. enterica serovar Typhimurium |
50000 | - |
alpha-subunit | Salmonella enterica subsp. enterica serovar Typhimurium |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ethanolamine | Salmonella enterica subsp. enterica serovar Typhimurium | elimination of ammonia from the vicinal position of short chain amino alcohol to give the corresponding oxo product | acetaldehyde + NH3 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Salmonella enterica subsp. enterica serovar Typhimurium | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Salmonella enterica subsp. enterica serovar Typhimurium |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
specific activity is 30-45 micromol ethanolamine/min/mg of EAL at 25°C, determined by the EAL-alcohol dehydrogenase coupled assay | Salmonella enterica subsp. enterica serovar Typhimurium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ethanolamine | elimination of ammonia from the vicinal position of short chain amino alcohol to give the corresponding oxo product | Salmonella enterica subsp. enterica serovar Typhimurium | acetaldehyde + NH3 | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | functional protein is a hexamer of alpha-beta-dimers (alpha-subunit of 50000 Da and beta-subunit of 31000 Da) | Salmonella enterica subsp. enterica serovar Typhimurium |
Synonyms | Comment | Organism |
---|---|---|
EAL | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
ethanolamine ammonia-lyase | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Salmonella enterica subsp. enterica serovar Typhimurium |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
coenzyme B12 | adenosylcobalamin, 70% of the enzyme-bound adenosylcobalamin is cleaved to cob(2)-alamin in the steady state of the reaction | Salmonella enterica subsp. enterica serovar Typhimurium |