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Literature summary for 4.3.1.7 extracted from

  • Zhu, C.; Warncke, K.
    Reaction of the Co(II)-substrate radical pair catalytic intermediate in coenzyme B12-dependent ethanolamine ammonia-lyase in frozen aqueous solution from 190 to 217 K (2008), Biophys. J., 95, 5890-5900.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
cloning in Escherichia coli overexpression strain incorporating the cloned Salmonella typhimurium EAL coding sequence Salmonella enterica subsp. enterica serovar Typhimurium

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ included in the cofactor, essential for enzyme binding Salmonella enterica subsp. enterica serovar Typhimurium

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
500000
-
calculated for the holoenzyme Salmonella enterica subsp. enterica serovar Typhimurium

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ethanolamine Salmonella enterica subsp. enterica serovar Typhimurium
-
acetaldehyde + NH3
-
?

Organism

Organism UniProt Comment Textmining
Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Salmonella enterica subsp. enterica serovar Typhimurium

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
enzyme activity is determined by using the coupled assay with alcohol dehydrogenase/NADH, the specific activity of the purified enzyme with aminoethanol as substrate was 20-30 micromol/min/mg Salmonella enterica subsp. enterica serovar Typhimurium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ethanolamine
-
Salmonella enterica subsp. enterica serovar Typhimurium acetaldehyde + NH3
-
?

Synonyms

Synonyms Comment Organism
EAL
-
Salmonella enterica subsp. enterica serovar Typhimurium
ethanolamine ammonia-lyase
-
Salmonella enterica subsp. enterica serovar Typhimurium

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
additional information the steady-state accumulation, and subsequent cryotrapping, of the Co2-substrate radical pair as the only detectable paramagnetic intermediate, suggest that the step after Co2-substrate radical pair formation is at least partially rate limiting for steady-state turnover Salmonella enterica subsp. enterica serovar Typhimurium

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Salmonella enterica subsp. enterica serovar Typhimurium

Cofactor

Cofactor Comment Organism Structure
coenzyme B12 adenosylcobalamin Salmonella enterica subsp. enterica serovar Typhimurium