Cloned (Comment) | Organism |
---|---|
cloning in Escherichia coli overexpression strain incorporating the cloned Salmonella typhimurium EAL coding sequence | Salmonella enterica subsp. enterica serovar Typhimurium |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | included in the cofactor, essential for enzyme binding | Salmonella enterica subsp. enterica serovar Typhimurium |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
500000 | - |
calculated for the holoenzyme | Salmonella enterica subsp. enterica serovar Typhimurium |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ethanolamine | Salmonella enterica subsp. enterica serovar Typhimurium | - |
acetaldehyde + NH3 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Salmonella enterica subsp. enterica serovar Typhimurium | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Salmonella enterica subsp. enterica serovar Typhimurium |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
enzyme activity is determined by using the coupled assay with alcohol dehydrogenase/NADH, the specific activity of the purified enzyme with aminoethanol as substrate was 20-30 micromol/min/mg | Salmonella enterica subsp. enterica serovar Typhimurium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ethanolamine | - |
Salmonella enterica subsp. enterica serovar Typhimurium | acetaldehyde + NH3 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
EAL | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
ethanolamine ammonia-lyase | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | the steady-state accumulation, and subsequent cryotrapping, of the Co2-substrate radical pair as the only detectable paramagnetic intermediate, suggest that the step after Co2-substrate radical pair formation is at least partially rate limiting for steady-state turnover | Salmonella enterica subsp. enterica serovar Typhimurium |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Salmonella enterica subsp. enterica serovar Typhimurium |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
coenzyme B12 | adenosylcobalamin | Salmonella enterica subsp. enterica serovar Typhimurium |