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Literature summary for 4.3.1.7 extracted from

  • Blackwell, C.M.; Turner, J.M.
    Microbial metabolism of amino alcohols. Purification and properties of coenzyme B12-dependent ethanolamine ammonia-lyase of Escherichia coli (1978), Biochem. J., 175, 555-563.
    View publication on PubMedView publication on EuropePMC

General Stability

General Stability Organism
ethanolamine, dithiothreitol, glycerol and KCl protect the apoenzyme from inactivation Escherichia coli
K+ stabilizes the enzyme during dialysis or storage Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
cyanocobalamin irreversible Escherichia coli
DL-1,3-diaminopropan-2-ol competitive Escherichia coli
DL-1-aminopropan-2-ol competitive Escherichia coli
hydroxycobalamin irreversible; noncompetitive Escherichia coli
iodoacetamide in absence of ethanolamine Escherichia coli
L-2-Aminopropan-1-ol competitive Escherichia coli
methylcobalamin irreversible Escherichia coli
p-hydroxymercuribenzoate 0.0001 mM, 50% inhibition; even in the presence of ethanolamine Escherichia coli
Urea above 1 mM Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.03
-
ethanolamine pH 7 Escherichia coli
0.3
-
ethanolamine pH 5.8 Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
K+ not required Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
560400
-
gel filtration Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
13.9
-
-
Escherichia coli

Storage Stability

Storage Stability Organism
-20°C, Tris/HCl buffer supplemented with glycerol, dithiothreitol, KCl and ethanolamine, stable for several months Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ethanolamine
-
Escherichia coli acetaldehyde + NH3
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 8
-
Escherichia coli

Cofactor

Cofactor Comment Organism Structure
vitamin B12 Km: 0.00042 mM Escherichia coli

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0001
-
p-hydroxymercuribenzoate
-
Escherichia coli