General Stability | Organism |
---|---|
ethanolamine, dithiothreitol, glycerol and KCl protect the apoenzyme from inactivation | Escherichia coli |
K+ stabilizes the enzyme during dialysis or storage | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
cyanocobalamin | irreversible | Escherichia coli | |
DL-1,3-diaminopropan-2-ol | competitive | Escherichia coli | |
DL-1-aminopropan-2-ol | competitive | Escherichia coli | |
hydroxycobalamin | irreversible; noncompetitive | Escherichia coli | |
iodoacetamide | in absence of ethanolamine | Escherichia coli | |
L-2-Aminopropan-1-ol | competitive | Escherichia coli | |
methylcobalamin | irreversible | Escherichia coli | |
p-hydroxymercuribenzoate | 0.0001 mM, 50% inhibition; even in the presence of ethanolamine | Escherichia coli | |
Urea | above 1 mM | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | - |
ethanolamine | pH 7 | Escherichia coli | |
0.3 | - |
ethanolamine | pH 5.8 | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | not required | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
560400 | - |
gel filtration | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
13.9 | - |
- |
Escherichia coli |
Storage Stability | Organism |
---|---|
-20°C, Tris/HCl buffer supplemented with glycerol, dithiothreitol, KCl and ethanolamine, stable for several months | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ethanolamine | - |
Escherichia coli | acetaldehyde + NH3 | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 8 | - |
Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
vitamin B12 | Km: 0.00042 mM | Escherichia coli |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0001 | - |
p-hydroxymercuribenzoate | - |
Escherichia coli |